Genetic characterization of a red colour morph of Euphorbia esula subsp. esula (Euphorbiaceae) in the floodplains of the Saône (Eastern France)

Botany ◽  
2016 ◽  
Vol 94 (10) ◽  
pp. 1001-1007
Author(s):  
Matthew Augé ◽  
Marie-Claude Bon ◽  
Laurent Hardion ◽  
Thomas Le Bourgeois ◽  
René F.H. Sforza
Keyword(s):  
North America ◽  
Chloroplast Dna ◽  
Genetic Characterization ◽  
Sequence Data ◽  
Leafy Spurge ◽  
Euphorbia Esula ◽  
Colour Morph ◽  
The Common ◽  
Region Form

A new morph of leafy spurge, Euphorbia esula L., was discovered in the floodplains of the Val de Saône (VDS), France. In this region, this emerging weed shows two different morph colours: the common “green” variant and a rarer “red” variant. We compared the sequence data for the plastid trnT–trnF region among individuals of both morphs from the VDS, as well as other populations from Europe and North America, where this plant is invasive. In addition, we analysed the ploidy found among VDS individuals for both colours. Results showed that both morphs share two of the three chloroplast DNA (cpDNA) haplotypes found in the VDS and are both hexaploids (2n = 6x = 60). These results suggest that the red morph is only an environmental variant of E. esula subsp. esula. Our results indicate that leafy spurge infestations in this region form a genetically diverse structuring composed of three cpDNA haplotypes, two of which are shared by the two morphs.

scholarly journals Subtype Characterization and Zoonotic Potential of Cryptosporidium felis in Cats in Guangdong and Shanghai, China

Pathogens ◽  
2021 ◽  
Vol 10 (2) ◽  
pp. 89
Author(s):  
Jiayu Li ◽  
Fuxian Yang ◽  
Ruobing Liang ◽  
Sheng Guo ◽  
Yaqiong Guo ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Phylogenetic Analysis ◽  
Genetic Characterization ◽  
Zoonotic Potential ◽  
Common Occurrence ◽  
High Genetic Diversity ◽  
Gp60 Gene ◽  
The Common ◽  
Subtyping Tool

Cryptosporidiumfelis is an important cause of feline and human cryptosporidiosis. However, the transmission of this pathogen between humans and cats remains controversial, partially due to a lack of genetic characterization of isolates from cats. The present study was conducted to examine the genetic diversity of C. felis in cats in China and to assess their potential zoonotic transmission. A newly developed subtyping tool based on a sequence analysis of the 60-kDa glycoprotein (gp60) gene was employed to identify the subtypes of 30 cat-derived C. felis isolates from Guangdong and Shanghai. Altogether, 20 C. felis isolates were successfully subtyped. The results of the sequence alignment showed a high genetic diversity, with 13 novel subtypes and 2 known subtypes of the XIXa subtype family being identified. The known subtypes were previously detected in humans, while some of the subtypes formed well-supported subclusters with human-derived subtypes from other countries in a phylogenetic analysis of the gp60 sequences. The results of this study confirmed the high genetic diversity of the XIXa subtype family of C. felis. The common occurrence of this subtype family in both humans and cats suggests that there could be cross-species transmission of C. felis.

scholarly journals Release of Postsenescent Dormancy in Leafy Spurge (Euphorbia esula) by Chilling

Weed Science ◽  
1988 ◽  
Vol 36 (6) ◽  
pp. 784-786 ◽  
Author(s):  
Stephen J. Harvey ◽  
Robert M. Nowierski
Keyword(s):  
Growth Rate ◽  
North America ◽  
Growth And Development ◽  
Leafy Spurge ◽  
Climatic Conditions ◽  
Euphorbia Esula ◽  
Flower Buds ◽  
Significant Growth ◽  
Number Of Stems

The growth and development of leafy spurge (Euphorbia esulaL. #3EPHES) collected during postsenescent dormancy and grown in the greenhouse was increasingly stimulated by chilling treatments longer than 14 days duration at 0 to 6 C. Production of stems with flower buds, primary flowers, and secondary flowers was greater in plants chilled for 42 days or more. The effects of chilling on total number of stems, number of strictly vegetative stems, or number of stems with vegetative branching were not significant. The height of the tallest stem per pot was influenced by chilling longer than 42 days. Growth rate also increased as a function of chilling duration. Based on our findings, we believe that there is little possibility that any significant growth can occur in the postsenescent period because of the prevailing climatic conditions found in areas of leafy spurge distribution in North America.

Genetic Characterization of Toxoplasma gondii DNA Samples Isolated From Humans Living in North America: An Unexpected High Prevalence of Atypical Genotypes

2018 ◽  
Vol 218 (11) ◽  
pp. 1783-1791 ◽  
Author(s):  
Christelle Pomares ◽  
Sébastien Devillard ◽  
Tyson H Holmes ◽  
Tudor Rares Olariu ◽  
Cynthia J Press ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
North America ◽  
Genetic Characterization ◽  
High Prevalence

Genetic characterization of Rhizobium sp. strain TAL1145 that nodulates tree legumes

1994 ◽  
Vol 40 (3) ◽  
pp. 208-215 ◽  
Author(s):  
M. L. C. George ◽  
J. P. W. Young ◽  
D. Borthakur
Keyword(s):  
Rhizobium Leguminosarum ◽  
Genetic Characterization ◽  
Rhizobium Meliloti ◽  
Wild Type ◽  
Tree Legumes ◽  
Wide Range ◽  
The Common ◽  
Rrna Sequences ◽  
Rhizobium Sp

Rhizobium sp. strain TALI 145 nodulates Leucaena ieucocephaia and Phaseolus vulgaris, in addition to a wide range of tropical tree legumes. Six overlapping clones that complemented nodulation defects in leucaena and bean rhizobia were isolated and a 40-kb map of the symbiosis region was constructed. The common nod and nifA genes were situated approximately 17 kb apart, with the nodlJ genes in between. These clones enabled a derivative of TAL1145 carrying a partially deleted pSym to form ineffective nodules on both leucaena and bean, and a similar derivative of Rhizobium etli TAL182 to form ineffective nodules on bean. When two representative clones, pUHR9 and pUHR114, were each transferred to wild-type rhizobial strains, they allowed ineffective nodulation by Rhizobium meliloti on both leucaena and bean and by Rhizobium leguminosarum bv. viciae on bean. Transconjugants of R. leguminosarum bv. trifolii formed effective nodules on leucaena and ineffective nodules on bean. Tn5 mutagenesis of the symbiosis region resulted in a variety of nodulation and fixation phenotypes on leucaena and bean. On the basis of 16S rRNA sequences, TAL1145 was found to be distinct from both R. tropici and NGR234, the two groups of leucaena symbionts that were previously described.Key words: Rhizobium, Leucaena leucocephala, nodulation, nitrogen fixation.

scholarly journals Genetic Characterization of Flowering Cherries (Prunus subgenus Cerasus) Using rp116-rp114 Spacer Sequences of Chloroplast DNA

2006 ◽  
Vol 75 (1) ◽  
pp. 72-78 ◽  
Author(s):  
Satoshi Ohta ◽  
Shinsuke Osumi ◽  
Toshio Katsuki ◽  
Ikuo Nakamura ◽  
Toshiya Yamamoto ◽  
...  
Keyword(s):  
Chloroplast Dna ◽  
Genetic Characterization

Genetic characterization of pear varieties revealed by chloroplast DNA sequences

2003 ◽  
Vol 78 (2) ◽  
pp. 241-247 ◽  
Author(s):  
T. Kimura ◽  
H. Iketani ◽  
K. Kotobuki ◽  
N. Matsuta ◽  
Y. Ban ◽  
...  
Keyword(s):  
Chloroplast Dna ◽  
Dna Sequences ◽  
Genetic Characterization ◽  
Chloroplast Dna Sequences

Morphometric studies of the Euphorbia esula group (Euphorbiaceae) in North America

1990 ◽  
Vol 68 (9) ◽  
pp. 1978-1988 ◽  
Author(s):  
C. W. Crompton ◽  
A. E. Stahevitch ◽  
W. A. Wojtas
Keyword(s):  
North America ◽  
Morphological Characters ◽  
Leafy Spurge ◽  
Principal Coordinate Analysis ◽  
Euphorbia Esula ◽  
Eastern North America ◽  
Single Variable ◽  
Authentic Material ◽  
Variable Species ◽  
Euphorbia Cyparissias

Leafy spurge and cypress spurge are important weeds of pastures and unimproved rangeland in western and eastern North America, respectively. Taxonomists disagree as to whether leafy spurge is a single variable species or an aggregate of two or more species. Morphometric techniques (clustering by incremental sum of squares and principal coordinate analysis) were used to analyze relationships in leafy spurge and its allies. On the basis of studying 26 morphological characters found in 200 collections representing 32 putative taxa, we concluded that in North America only four species should be recognized, namely, Euphorbia agraria Bieb., Euphorbia cyparissias L., Euphorbia esula L., and Euphorbia ×pseudoesula Schur. A key to these taxa is provided. No authentic material of Euphorbia lucida Waldst. & Kitt. and Euphorbia salicifolia Host was found from this continent in the collections we examined.

scholarly journals Isolation and genetic characterization of a relapsing fever spirochete isolated from Ornithodoros puertoricensis collected in central Panama

2021 ◽  
Vol 15 (8) ◽  
pp. e0009642
Author(s):  
Sergio E. Bermúdez ◽  
Brittany A. Armstrong ◽  
Lillian Domínguez ◽  
Aparna Krishnavajhala ◽  
Alexander R. Kneubehl ◽  
...  
Keyword(s):  
Latin America ◽  
North America ◽  
Central America ◽  
Human Disease ◽  
Genetic Characterization ◽  
Relapsing Fever ◽  
Tick Vector ◽  
The City ◽  
Central Panama

Tick-borne relapsing fever (TBRF) spirochetes are likely an overlooked cause of disease in Latin America. In Panama, the pathogens were first reported to cause human disease in the early 1900s. Recent collections of Ornithodoros puertoricensis from human dwellings in Panama prompted our interest to determine whether spirochetes still circulate in the country. Ornithodoros puertoricensis ticks were collected at field sites around the City of Panama. In the laboratory, the ticks were determined to be infected with TBRF spirochetes by transmission to mice, and we report the laboratory isolation and genetic characterization of a species of TBRF spirochete from Panama. Since this was the first isolation of a species of TBRF spirochete from Central America, we propose to designate the bacteria as Borrelia puertoricensis sp. nov. This is consistent with TBRF spirochete species nomenclature from North America that are designated after their tick vector. These findings warrant further investigations to assess the threat B. puertoricensis sp. nov. may impose on human health.

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