The effect of gram-positive (Desulfosporosinus orientis) and gram-negative (Desulfovibrio desulfuricans) sulfate-reducing bacteria on iron sulfide mineral precipitation

2018 ◽  
Vol 64 (9) ◽  
pp. 629-637 ◽  
Author(s):  
William Stanley ◽  
Gordon Southam
Keyword(s):  
Electron Microscopy ◽  
Iron Sulfide ◽  
Desulfovibrio Desulfuricans ◽  
Sulfate Reducing Bacteria ◽  
Sulfide Mineral ◽  
Gram Positive ◽  
Gram Negative ◽  
Sulfate Reducing ◽  
Cell Autolysis ◽  
Reducing Bacteria

Growth of two dissimilatory sulfate-reducing bacteria, Desulfosporosinus orientis (gram-positive) and Desulfovibrio desulfuricans (gram-negative), in a chemically defined culture medium resulted in similar growth rates (doubling times for each culture = 2.8 h) and comparable rates of H2S generation (D. orientis = 0.19 nmol/L S2–per cell per h; D. desulfuricans = 0.12 nmol/L S2–per cell per h). Transmission electron microscopy of whole mounts and thin sections revealed that the iron sulfide mineral precipitates produced by the two cultures were morphologically different. The D. orientis culture flocculated, with the minerals occurring as subhedral plate-like precipitates, which nucleated on the cell wall during exponential growth producing extensive mineral aggregates following cell autolysis and endospore release. In contrast, the D. desulfuricans culture produced fine-grained colloidal or platy iron sulfide precipitates primarily within the bulk solution. Mineral analysis by scanning electron microscopy – energy dispersive spectroscopy indicated that neither culture promoted advanced mineral development beyond a 1:1 Fe:S stoichiometry. This analysis did not detect pyrite (FeS2). The average Fe:S ratios were 1 : 1.09 ± 0.03 at 24 h and 1 : 1.08 ± 0.03 at 72 h for D. orientis and 1 : 1.05 ± 0.02 at 24 h and 1 : 1.09 ± 0.07 at 72 h for D. desulfuricans. The formation of “biogenic” iron sulfides by dissimilatory sulfate-reducing bacteria is influenced by bacterial cell surface structure, chemistry, and growth strategy, i.e., mineral aggregation occurred with cell autolysis of the gram-positive bacterium.

Response of Desulfovibrio desulfuricans colonies to oxygen stress

1990 ◽  
Vol 36 (6) ◽  
pp. 400-408 ◽  
Author(s):  
Judy D. Wall ◽  
Barbara J. Rapp-Giles ◽  
Merton F. Brown ◽  
Jerry A. White
Keyword(s):  
Electron Microscopy ◽  
Morphological Changes ◽  
Metal Sulfide ◽  
Desulfovibrio Desulfuricans ◽  
Sulfate Reducing Bacteria ◽  
Strictly Anaerobic ◽  
Air Oxidation ◽  
Oxygen Tolerance ◽  
Sulfate Reducing ◽  
Reducing Bacteria

Oxygen tolerance of the strictly anaerobic sulfate-reducing bacteria is well documented and poorly understood. This capacity for surviving brief exposures to oxygen must be a major factor in the diversity of environmental niches observed for these bacteria. We observed that viable cells of Desulfovibrio desulfuricans (ATCC 27774) could be found in colonies on the surface of solidified medium exposed to air for periods as long as 1 month. During exposure to air, the originally black colonies became greyish white, presumably as a result of the air oxidation of the metal sulfide deposits. A black, brittle deposit formed at the bottom of the colony and, simultaneously, the colony descended into a dimple that developed into a well in the agar. Eventually the colony reached the bottom of the Petri dish. These changes did not take place when the colonies were maintained in an anaerobic chamber. The morphological changes took place with all strains tested: three strains of D. desulfuricans and one strain of Desulfovibrio gigas and Desulfovibrio multispirans. Continued sulfate reduction appeared to be essential. Cyclic sulfate (thiosulfate or sulfite) reduction to sulfide and reoxidation of sulfide by the oxygen in air are proposed to maintain the viability of the bacteria by providing substrates for energy production and by reducing oxygen tension. Scanning and transmission electron microscopy of colony and cellular changes are shown. Key words: Desulfovibrio, sulfate-reducing bacteria, oxygen tolerance, sulfate cycling, scanning electron microscopy.

Pyrite and the Global Environment

Pyrite ◽  
2015 ◽  
Author(s):  
David Rickard
Keyword(s):  
Iron Sulfide ◽  
Sulfide Oxidation ◽  
Sulfate Reducing Bacteria ◽  
Sulfide Mineral ◽  
Sulfur Cycle ◽  
Sulfate Reducing ◽  
Global Dynamic ◽  
Sulfur Oxidizing ◽  
Life On Earth ◽  
Reducing Bacteria

The two basic processes concerning pyrite in the environment are the formation of pyrite, which usually involves reduction of sulfate to sulfide, and the destruction of pyrite, which usually involves oxidation of sulfide to sulfate. On an ideal planet these two processes might be exactly balanced. But pyrite is buried in sediments sometimes for hundreds of millions of years, and the sulfur in this buried pyrite is removed from the system, so the balance is disturbed. The lack of balance between sulfide oxidation and sulfate reduction powers a global dynamic cycle for sulfur. This would be complex enough if this were the whole story. However, as we have seen, both the reduction and oxidation arms of the global cycle are essentially biological—specifically microbiological—processes. This means that there is an intrinsic link between the sulfur cycle and life on Earth. In this chapter, we examine the central role that pyrite plays, and has played, in determining the surface environment of the planet. In doing so we reveal how pyrite, the humble iron sulfide mineral, is a key component of maintaining and developing life on Earth. In Chapter 4 we concluded that Mother Nature must be particularly fond of pyrite framboids: a thousand billion of these microscopic raspberry-like spheres are formed in sediments every second. If we translate this into sulfur production, some 60 million tons of sulfur is buried as pyrite in sediments each year. But this is only a fraction of the total amount of sulfide produced every year by sulfate-reducing bacteria. In 1982 the Danish geomicrobiologist Bo Barker Jørgensen discovered that as much as 90% of the sulfide produced by sulfate-reducing bacteria was rapidly reoxidized by sulfur-oxidizing microorganisms. Sulfate-reducing microorganisms actually produce about 300 million tons of sulfur each year, but about 240 million tons is reoxidized. The magnitude of the sulfide production by sulfate-reducing bacte­ria can be appreciated by comparison with the sulfur produced by volcanoes. As discussed in Chapter 5, it was previously supposed that all sulfur, and thus pyrite, had a volcanic origin. In fact volcanoes produce just 10 million tons of sulfur each year.

Sulfate-reducing bacteria and silica solubility: a possible mechanism for evaporite diagenesis and silica precipitation in banded iron formations

1985 ◽  
Vol 22 (12) ◽  
pp. 1904-1909 ◽  
Author(s):  
Stuart J. Birnbaum ◽  
John W. Wireman
Keyword(s):  
Desulfovibrio Desulfuricans ◽  
Sulfate Reducing Bacteria ◽  
Sulfide Mineral ◽  
Banded Iron Formations ◽  
Dissolved Silica ◽  
Sulfate Reducing ◽  
Silica Precipitation ◽  
Reducing Bacteria ◽  
Iron Formations

Selective replacement of sulfate-evaporite minerals by silica and the precipitation of silica in association with sulfide mineral phases in banded iron formations may be mediated by the metabolic activities of sulfate-reducing bacteria. Hydrogen sulfide is known to be a product of this metabolism and is often called upon as a source of sulfur for metallic sulfides in sedimentary rocks. We report here on the influence that chemical changes induced by bacterial sulfate reduction have on silica solubility.Controlled in vitro growth experiments with Desulfovibrio desulfuricans and silica show (1) this organism can grow in silica concentrations as great as 400 ppm with no inhibition and (2) growth in the presence of silica yields a decrease in dissolved silica.Growth experiments with 80 ppm silica produced a lowering in dissolved silica from 80 ppm to 60 ppm, a 25% decrease, in just 30 h. Control experiments in the absence of cells resulted in no effective decrease in dissolved silica. The ability of sulfate-reducing bacteria to remove silica from solution may be related to local changes in pH and to hydrogen bonding of amorphous silica followed by polymerization to higher weight molecules.

Specificity of Lectins Labeled with Colloidal Gold to the Exopolymeric Matrix Carbohydrates of the Sulfate-Reducing Bacteria Biofilm Formed on Steel

2020 ◽  
Vol 82 (5) ◽  
pp. 11-20
Author(s):  
D.R. Abdulina ◽  
◽  
L.M. Purish ◽  
G.O. Iutynska ◽  
◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Colloidal Gold ◽  
Steel Surface ◽  
Lectin Binding ◽  
Sulfate Reducing Bacteria ◽  
Gold Particles ◽  
Sulfate Reducing ◽  
Transmission Electron ◽  
Reducing Bacteria

The studies of the carbohydrate composition of the sulfate-reducing bacteria (SRB) biofilms formed on the steel surface, which are a factor of microbial corrosion, are significant. Since exopolymers synthesized by bacteria could activate corrosive processes. The aim of the study was to investigate the specificity of commercial lectins, labeled with colloidal gold to carbohydrates in the biofilm exopolymeric matrix produced by the corrosive-relevant SRB strains from man-caused ecotopes. Methods. Microbiological methods (obtaining of the SRB biofilms during cultivation in liquid Postgate B media under microaerophilic conditions), biochemical methods (lectin-binding analysis of 10 commercial lectins, labeled with colloidal gold), transmission electron microscopy using JEM-1400 JEOL. Results. It was shown using transmission electron microscopy that the binding of lectins with carbohydrates in the biofilm of the studied SRB strains occurred directly in the exopolymerіс matrix, as well as on the surfaces of bacterial cells, as seen by the presence of colloidal gold particles. For detection of the neutral carbohydrates (D-glucose and D-mannose) in the biofilm of almost all studied bacterial strains PSA lectin was the most specific. This lectin binding in biofilms of Desulfotomaculum sp. К1/3 and Desulfovibrio sp. 10 strains was higher in 90.8% and 94.4%, respectively, then for ConA lectin. The presence of fucose in the SRB biofilms was detected using LABA lectin, that showed specificity to the biofilm EPS of all the studied strains. LBA lectin was the most specific to N-аcetyl-D-galactosamine for determination of amino sugars in the biofilm. The amount of this lectin binding in D. vulgaris DSM644 biofilm was 30.3, 10.1 and 9.3 times higher than SBA, SNA and PNA lectins, respectively. STA, LVA and WGA lectins were used to detect the N-acetyl-Dglucosamine and sialic acid in the biofilm. WGA lectin showed specificity to N-acetyl-D-glucosamine in the biofilm of all the studied SRB; maximum number of bounded colloidal gold particles (175 particles/μm2) was found in the Desulfotomaculum sp. TC3 biofilm. STA lectin was interacted most actively with N-acetyl-D-glucosamine in Desulfotomaculum sp. TC3 and Desulfomicrobium sp. TC4 biofilms. The number of bounded colloidal gold particles was in 9.2 and 7.4 times higher, respectively, than using LVA lectin. The lowest binding of colloidal gold particles was observed for LVA lectin. Conclusions. It was identified the individual specificity of the 10 commercial lectins to the carbohydrates of biofilm matrix on the steel surface, produced by SRB. It was estimated that lectins with identical carbohydrates specificity had variation in binding to the biofilm carbohydrates of different SRB strains. Establishing of the lectin range selected for each culture lead to the reduction of the scope of studies and labor time in the researching of the peculiarities of exopolymeric matrix composition of biofilms formed by corrosiverelevant SRB.

Experimental Investigation on the Active Range of Sulfate-Reducing Bacteria for Geological Disposal

1994 ◽  
Vol 353 ◽  
Author(s):  
S. Fukunaga ◽  
H. Yoshikawa ◽  
K. Fujiki ◽  
H. Asano
Keyword(s):  
Experimental Investigation ◽  
Environmental Conditions ◽  
Desulfovibrio Desulfuricans ◽  
Sulfate Reducing Bacteria ◽  
Geological Disposal ◽  
Sulfate Reducing ◽  
Buffer Material ◽  
Ph Range ◽  
High Level ◽  
Reducing Bacteria

AbstractThe active range ofDesulfovibrio desulfuricans. a species of sulfate-reducing bacteria, was examined in terms of pH and Eh using a fermenter at controlled pH and Eh. Such research is important because sulfate-reducing bacteria (SRB) are thought to exist underground at depths equal to those of supposed repositories for high-level radioactive wastes and to be capable of inducing corrosion of the metals used in containment vessels.SRB activity was estimated at 35°C, with lactate as an electron donor, at a pH range from 7 to 11 and Eh range from 0 to -380 mV. Activity increased as pH approached neutral and Eh declined. The upper pH limit for activity was between 9.9 and 10.3, at Eh of -360 to -384 mV. The upper Eh limit for activity was between -68 and -3 mV, at pH 7.1. These results show that SRB can be made active at higher pH by decreasing Eh, and that the higher pH levels of 8 to 10 produced by use of the buffer material bentonite does not suppress SRB completely.A chart was obtained showing the active range ofDesulfovibrio desulfuricansin terms of pH and Eh. Such charts can be used to estimate the viability of SRB and other microorganisms when the environmental conditions of a repository are specified.

A comparison of the electrophoretic properties of the ATP-sulfurylases, APS-reductases, and sulfite reductases from cultures of dissimilatory sulfate-reducing bacteria

1973 ◽  
Vol 19 (3) ◽  
pp. 375-380 ◽  
Author(s):  
G. W. Skyring ◽  
P. A. Trudinger
Keyword(s):  
Desulfovibrio Desulfuricans ◽  
Sulfate Reducing Bacteria ◽  
Disc Electrophoresis ◽  
Sulfite Reductase ◽  
Electrophoretic Variation ◽  
Sulfate Reducing ◽  
Aps Reductase ◽  
Reducing Bacteria ◽  
Atp Sulfurylases

ATP-sulfurylases, APS-reductases, and sulfite reductases (SO3−2 → S−2) have been detected by gel disc electrophoresis in 13 cultures of dissimilatory sulfate-reducing bacteria and their electrophoretic properties have been compared. With respect to these three enzymes only, the results were indicative of some interspecies and intergenus homologies. In the Desulfovibrio strains (except Desulfovibrio desulfuricans 8301 which does not contain desulfoviridin), the major sulfite reductase was electrophoretically coincident with desulfoviridin and, in the Desulfotomaculum strains, with a brown protein. Some distinct patterns of electrophoretically distinguishable forms of APS-reductase were found. Considerable electrophoretic variation was found among the ATP-sulfurylases.

Quantitative ecology of psychrophilic bacteria in an aquatic environment and characterization of heterotrophic bacteria from permanently cold sediments

1979 ◽  
Vol 25 (12) ◽  
pp. 1433-1442 ◽  
Author(s):  
L. G. Leduc ◽  
G. D. Ferroni
Keyword(s):  
Incubation Temperature ◽  
Heterotrophic Bacteria ◽  
Sulfate Reducing Bacteria ◽  
Gram Positive ◽  
Sulfate Reducing ◽  
Quantitative Ecology ◽  
Physiological Group ◽  
Sulfur Oxidizing ◽  
Reducing Bacteria

Aerobic heterotrophic bacteria, anaerobic heterotrophic bacteria, ammonifying bacteria, sulfur-oxidizing bacteria, and sulfate-reducing bacteria were quantitated in Fairbank Lake, an oligotrophic to mesotrophic lake with a permanently cold hypolimnion, as a function of depth in three seasons. Representatives of each physiological group were recovered at an incubation temperature of 2 °C and for all the physiological groups the 2 °C counts were usually higher than the 37 °C counts, although sulfate-reducing bacteria were not recoverable at an incubation temperature of 37 °C. In addition, the numbers of each physiological type were generally higher in the sediments than in the water column, except in the case of sulfate-reducing bacteria for which the counts were low and often below the detection limit. Aerobic heterotrophic bacteria usually outnumbered the other physiological groups surveyed, and winter minima were characteristic of some of the physiological groups. A relatively stable density of anaerobic heterotrophic bacteria, as a function of sediment depth, was observed when the incubation temperature was 2 °C. At 37 °C, these anaerobes were not detected, and this was true for sulfate-reducing bacteria at both temperatures.Heterotrophic bacterial isolates from the permanently cold sediments were examined with regard to Gram reaction, the obligate or facultative nature of anaerobes, ability to use ecologically important substrates, psychrophilic type, and temperature range for growth. Isolates recovered at 2 °C were predominantly Gram-negative bacilli, whereas isolates recovered at 37 °C were predominantly Gram-positive bacilli. The anaerobic isolates were mainly Gram-positive bacilli regardless of the isolation temperature, and most of those examined were obligately anaerobic. Many of the isolates tested were positive for gelatinase, chitinase, amylase, and lipase, but none was positive for cellulase. Most of the sediment isolates were facultatively psychrophilic and a considerable fraction of the 37 °C isolates were facultative psychrophiles.

Contribution of Sulfate-Reducing Bacteria to the Electricity Generation in Microbial Fuel Cells

2014 ◽  
Vol 1008-1009 ◽  
pp. 285-289 ◽  
Author(s):  
Chong Yang Gao ◽  
Ai Jie Wang ◽  
Yang Guo Zhao
Keyword(s):  
Fuel Cells ◽  
Power Density ◽  
Microbial Fuel Cells ◽  
Electricity Generation ◽  
Desulfovibrio Desulfuricans ◽  
Sulfate Reducing Bacteria ◽  
Microbial Community Analysis ◽  
Electricity Production ◽  
Sulfate Reducing ◽  
Reducing Bacteria

Double-chambered microbial fuel cells (MFCs) were used to investigate the effect of sulfate and sulfate-reducing bacteria (SRB) on electricity generation by molybdate inhibition coupled with PCR-DGGE technique. Results showed that low influent sulfate (< 1470 mg/L) improved power density and voltage, while higher sulfate blocked the MFC efficiency. Molybdate inhibited the activity of SRB and consequently decreased MFC voltage and power density which confirmed some SRB were involved in the electricity generation. Microbial community analysis indicated thatDesulfovibrio desulfuricanscontributed to the electricity production and stability of MFC.

scholarly journals Reduction of perchlorate ions by the sulfate-reducing bacteria Desulfotomaculum sp. and Desulfovibrio desulfuricans

2020 ◽  
Vol 11 (2) ◽  
pp. 278-282
Author(s):  
N. S. Verkholiak ◽  
T. B. Peretyatko ◽  
A. A. Halushka
Keyword(s):  
Sewage Treatment ◽  
Desulfovibrio Desulfuricans ◽  
Sulfate Reducing Bacteria ◽  
Inhibitory Effect ◽  
Electron Acceptors ◽  
Wood Chips ◽  
Sulfate Ions ◽  
Sulfate Reducing ◽  
Perchlorate Ion ◽  
Reducing Bacteria

The usage of microorganisms to clean the environment from xenobiotics, in particular chlorine-containing ones, is a promising method of detoxifying the contaminated environment. Sulfate-reducing bacteria Desulfovibrio desulfuricans Ya-11, isolated from Yavoriv Lake, and Desulfotomaculum AR1, isolated from the Lviv sewage treatment system, are able to grow under conditions of environmental contamination by aromatic compounds and chlorine-containing substances. Due to their high redox potential, chlorate and perchlorate ions can be ideal electron acceptors for the metabolism of microorganisms. To test the growth of the tested microorganisms under the influence of perchlorate ions, bacteria were cultured in modified Postgate C medium with ClO4–. Biomass was determined turbidimetrically, the content of sulfate ions and hydrogen sulfide – photoelectrocolorimetrically, the content of perchlorate ions – permanganatometrically. The study of the ability of sulfate-reducing bacteria Desulfotomaculum AR1 and D. desulfuricans Ya-11 to grow in a medium with perchlorate ions as electron acceptors showed the inhibitory effect of ClO4– on sulfate ion reduction by bacteria. Bacteria Desulfotomaculum AR1 and D. desulfuricans Ya-11 are able to grow in environments with aromatic hydrocarbons, in particular toluene. The possibility of the growth of sulfate-reducing bacteria in the presence of toluene as an electron donor and perchlorate ions as an electron acceptor was investigated. The efficiency of perchlorate ion utilization by sulfate-reducing bacteria Desulfotomaculum AR1 and D. desulfuricans Ya-11 was about 90 %. The effect of molybdenum on the reduction of perchlorate ions by Desulfotomaculum AR1 is shown in the paper. Immobilization of bacteria Desulfotomaculum AR1 and D. desulfuricans Ya-11 was carried out in 3% agar and on wood chips. The ability of bacteria, immobilized on these media, to purify the aqueous medium from perchlorate ions was investigated. Reduction of perchlorate ions is more efficiently performed by cells of Desulfotomaculum AR1 and D. desulfuricans Ya-11 bacteria immobilized in agar than on wood chips. Sulfate-reducing bacteria Desulfotomaculum AR1 and D. desulfuricans Ya-11 are able to use perchlorate ions as electron acceptors, purifying the polluted aquatic environment from these pollutants.

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