Effect of transferring 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase genes into Pseudomonas fluorescens strain CHA0 and its gacA derivative CHA96 on their growth-promoting and disease-suppressive capacities

2000 ◽  
Vol 46 (10) ◽  
pp. 898-907 ◽  
Author(s):  
Chunxia Wang ◽  
Edouard Knill ◽  
Bernard R. Glick ◽  
Geneviève Défago
Keyword(s):  
Carboxylic Acid ◽  
Pseudomonas Fluorescens ◽  
Acc Deaminase ◽  
Growth Promoting

Involvement ofgacSandrpoSin enhancement of the plant growth-promoting capabilities ofEnterobacter cloacaeCAL2 and UW4

2001 ◽  
Vol 47 (8) ◽  
pp. 698-705 ◽  
Author(s):  
Saleema S Saleh ◽  
Bernard R Glick
Keyword(s):  
Plant Growth ◽  
Pseudomonas Fluorescens ◽  
Indoleacetic Acid ◽  
Acc Deaminase ◽  
Enterobacter Cloacae ◽  
Lag Phase ◽  
Plant Growth Promoting Bacteria ◽  
Multicopy Plasmid ◽  
Plant Growth Promoting ◽  
Growth Promoting

The plant growth-promoting bacteria Enterobacter cloacae CAL2 and UW4 were genetically transformed with a multicopy plasmid containing an rpoS or gacS gene from Pseudomonas fluorescens. The transformed strains were compared with the nontransformed strains for growth, indoleacetic acid (IAA) production, antibiotic production, 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase activity, siderophore production, cell morphology, and the ability to promote canola root elongation. All transformed strains had a longer lag phase, were slower in reaching stationary phase, and attained a higher cell density than the nontransformed strains. Transformation resulted in cells that were significantly shorter than the nontransformed cells. The transformed strains also produced significantly more IAA than the nontransformed strains. Introduction of rpoS or gacS from Pseudomonas fluorescens was associated with a reduction in the production of both antibiotics, 2,4-diacetylphloroglucinol and mono-acetylphloroglucinol, produced by Enterobacter cloacae CAL2. With Enterobacter cloacae CAL2, plasmid-borne rpoS, but not gacS, increased the level of ACC deaminase activity, while introduction of rpoS in Enterobacter cloacae UW4 caused a decrease in ACC deaminase activity. Neither gacS nor rpoS significantly affected the level of siderophores synthesized by either bacterial strain. Overproduction of either GacA or RpoS in Enterobacter cloacae CAL2 resulted in a significant increase in the root lengths of canola seedlings when seeds were treated with the bacteria, and overproduction of RpoS caused an increase in canola shoot as well as root lengths.Key words: plant growth-promoting bacteria, canola, ethylene, ACC deaminase, GacS, RpoS, indoleacetic acid, siderophores, antibiotics.

scholarly journals Genome Sequence of the Banana Plant Growth-Promoting Rhizobacterium Pseudomonas fluorescens PS006

2016 ◽  
Vol 4 (3) ◽  
Author(s):  
Rocío M. Gamez ◽  
Fernando Rodríguez ◽  
Sandra Ramírez ◽  
Yolanda Gómez ◽  
Richa Agarwala ◽  
...  
Keyword(s):  
Plant Growth ◽  
Pseudomonas Fluorescens ◽  
Genome Sequence ◽  
Acc Deaminase ◽  
Whole Genome Sequence ◽  
Genome Sequences ◽  
Banana Plant ◽  
Toxic Compounds ◽  
Plant Growth Promoting ◽  
Growth Promoting

Pseudomonas fluorescens is a well-known plant growth-promoting rhizobacterium (PGPR). We report here the first whole-genome sequence of PGPR P. fluorescens evaluated in Colombian banana plants. The genome sequences contains genes involved in plant growth and defense, including bacteriocins, 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase, and genes that provide resistance to toxic compounds.

Determination of 1-aminocycopropane-1-carboxylic acid (ACC) to assess the effects of ACC deaminase-containing bacteria on roots of canola seedlings

2001 ◽  
Vol 47 (1) ◽  
pp. 77-80 ◽  
Author(s):  
Donna M Penrose ◽  
Barbara A Moffatt ◽  
Bernard R Glick
Keyword(s):  
Carboxylic Acid ◽  
Plant Growth ◽  
Root Elongation ◽  
Acc Deaminase ◽  
Plant Growth Promoting Bacteria ◽  
Psychrophilic Bacterium ◽  
Ethylene Inhibitor ◽  
Seedling Roots ◽  
Plant Growth Promoting ◽  
Growth Promoting

Previously, it was proposed that plant growth-promoting bacteria that possess the enzyme, 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase, can reduce the amount of ethylene produced by a plant and thereby promote root elongation. To test this model, canola seeds were imbibed in the presence of the chemical ethylene inhibitor, 2-aminoethoxyvinyl glycine (AVG), various strains of plant growth-promoting bacteria, and a psychrophilic bacterium containing an ACC deaminase gene on a broad host range plasmid. The extent of root elongation and levels of ACC, the immediate precursor of ethylene, were measured in the canola seedling roots. A modification of the Waters AccQ*Tag Amino Acid Analysis Method(tm) was used to quantify ACC in the root extracts. It was found that, in the presence of the ethylene inhibitor, AVG, or any one of several ACC deaminase-containing strains of bacteria, the growth of canola seedling roots was enhanced and the ACC levels in these roots were lowered.

Effect of transferring 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase genes intoPseudomonas fluorescensstrain CHA0 and itsgacA derivative CHA96 on their growth-promoting and disease-suppressive capacities

2000 ◽  
Vol 46 (10) ◽  
pp. 898-907 ◽  
Author(s):  
Chunxia Wang ◽  
Edouard Knill ◽  
Bernard R Glick ◽  
Geneviève Défago
Keyword(s):  
Carboxylic Acid ◽  
Root Length ◽  
Regulatory Region ◽  
Acc Deaminase ◽  
Soft Rot ◽  
Plant Diseases ◽  
Potato Tubers ◽  
Experimental Conditions ◽  
Growth Promoting

Pseudomonas fluorescens strain CHA0, a root colonizing bacterium, has a broad spectrum of biocontrol activity against plant diseases. However, strain CHA0 is unable to utilize 1-aminocyclopropane-1-carboxylic acid (ACC), the immediate precursor of plant ethylene, as a sole source of nitrogen. This suggests that CHA0 does not contain the enzyme ACC deaminase, which cleaves ACC to ammonia and α-ketobutyrate, and was previously shown to promote root elongation of plant seedlings treated with bacteria containing this enzyme. An ACC deaminase gene, together with its regulatory region, was transferred into P. fluorescens strains CHA0 and CHA96, a global regulatory gacA mutant of CHA0. ACC deaminase activity was expressed in both CHA0 and CHA96. Transformed strains with ACC deaminase activity increased root length of canola plants under gnotobiotic conditions, whereas strains without this activity had no effect. Introduction of ACC deaminase genes into strain CHA0 improved its ability to protect cucumber against Pythium damping-off, and potato tubers against Erwinia soft rot in small hermetically sealed containers. In contrast, ACC deaminase activity had no significant effect on the ability of CHA0 to protect tomato against Fusarium crown and root rot, and potato tubers against soft rot in large hermetically sealed containers. These results suggest that (i) ACC deaminase activity may have lowered the level of plant ethylene thereby increasing root length; (ii) the role of stress-generated plant ethylene in susceptibility or resistance depends on the host-pathogen system, and on the experimental conditions used; and (iii) the constructed strains could be developed as biosensors for the role of ethylene in plant diseases.Key words: biocontrol, plant growth-promoting rhizobacteria, ACC deaminase, ethylene biosensors.

Inducing salt tolerance in mung bean through coinoculation with rhizobia and plant-growth-promoting rhizobacteria containing 1-aminocyclopropane-1-carboxylate deaminase

2011 ◽  
Vol 57 (7) ◽  
pp. 578-589 ◽  
Author(s):  
Maqshoof Ahmad ◽  
Zahir A. Zahir ◽  
H. Naeem Asghar ◽  
M. Asghar
Keyword(s):  
Salt Tolerance ◽  
Plant Growth ◽  
Pseudomonas Fluorescens ◽  
Mung Bean ◽  
Acc Deaminase ◽  
Plant Growth Promoting Rhizobacteria ◽  
Saline Stress ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Axenic Conditions

Twenty-five strains of plant-growth-promoting rhizobacteria (PGPR) containing 1-aminocyclopropane-1-carboxylate (ACC) deaminase and 10 strains of rhizobia were isolated from rhizosphere soil samples and nodules of mung bean. They were screened in separate trials under salt-stressed axenic conditions. The three most effective strains of PGPR (Mk1, Pseudomonas syringae ; Mk20, Pseudomonas fluorescens ; and Mk25, Pseudomonas fluorescens biotype G) and Rhizobium phaseoli strains M1, M6, and M9 were evaluated in coinoculation for their growth-promoting activity at three salinity levels (original, 4 dS·m–1, and 6 dS·m–1) under axenic conditions. The results showed that salinity stress significantly reduced plant growth but inoculation with PGPR containing ACC deaminase and rhizobia enhanced plant growth, thus reducing the inhibitory effect of salinity. However, their combined application was more effective under saline conditions, and the combination Mk20 × M6 was the most efficient for improving seedling growth and nodulation. The effect of high ethylene concentrations on plant growth and the performance of these strains for reducing the negative impact of saline stress was also evaluated by conducting a classical triple-response bioassay. The intensity of the classical triple response decreased owing to inoculation with these strains, with the root and shoot lengths of inoculated mung bean seedlings increasing and stem diameter decreasing, which is a typical response to the dilution in a classical triple response bioassay. Thus, coinoculation with PGPR containing ACC deaminase and Rhizobium spp. could be a useful approach for inducing salt tolerance and thus improving growth and nodulation in mung bean under salt-affected conditions.

1-Aminocyclopropane-1-carboxylic acid deaminase mutants of the plant growth promoting rhizobacterium Pseudomonas putida GR12-2 do not stimulate canola root elongation

1994 ◽  
Vol 40 (11) ◽  
pp. 911-915 ◽  
Author(s):  
Bernard R. Glick ◽  
Christian B. Jacobson ◽  
Melinda M. K. Schwarze ◽  
J. J. Pasternak
Keyword(s):  
Carboxylic Acid ◽  
Plant Growth ◽  
Pseudomonas Putida ◽  
Root Elongation ◽  
Acc Deaminase ◽  
Plant Growth Promoting Rhizobacteria ◽  
Wild Type ◽  
Ethylene Concentration ◽  
Plant Growth Promoting ◽  
Growth Promoting

The plant growth promoting rhizobacterium Pseudomonas putida GR12-2 was mutagenized with nitrosoguanidine and three separate mutants that were unable to utilize 1-aminocyclopropane-1-carboxylic acid (ACC) as a sole nitrogen source were selected. These mutants are devoid of the ACC deaminase activity that is present in wild-type P. putida GR12-2 cells. Only wild-type cells, but not any of the ACC deaminase mutants, promoted root elongation of developing canola seedlings under gnotobiotic conditions. These results are interpreted in terms of a model in which P. putida GR12-2 promotes root elongation by binding to germinating seeds and sequesters and hydrolyzes some of the unbound ACC, thereby lowering the level of ACC and hence the endogenous ethylene concentration, allowing the roots to grow longer.Key words: 1-aminocyclopropane-1-carboxylate, ACC, plant growth promoting rhizobacteria, PGPR, ACC deaminase, bacterial fertilizer.

scholarly journals Screening, Identification and Growth-Promotion Products of Multifunctional Bacteria in a Chinese Fir Plantation

Forests ◽  
2021 ◽  
Vol 12 (2) ◽  
pp. 120
Author(s):  
Guangyu Zhao ◽  
Yihui Wei ◽  
Jiaqi Chen ◽  
Yuhong Dong ◽  
Lingyu Hou ◽  
...  
Keyword(s):  
High Efficiency ◽  
Growth Promotion ◽  
Nitrogenase Activity ◽  
Acc Deaminase ◽  
Inorganic Phosphorus ◽  
Chinese Fir ◽  
Biochemical Tests ◽  
Growth Promoting ◽  
Chinese Fir Plantation ◽  
Physiological And Biochemical

Purpose: This research was aimed to screen and identify multifunctional phosphorus-dissolving bacteria of a Chinese fir (Cunninghamia lanceolata) plantation and study its phosphorus-dissolving characteristics in order to provide strain resources and a theoretical basis for developing the appropriate bacterial fertilizer of a Chinese fir plantation. Methods: First, phosphorus-dissolving bacteria were isolated from the woodland soil of a Chinese fir plantation by Pikovskava inorganic phosphorus medium (PVK). Then, some growth-promoting indicators of primary screening strains were determined, including the capacity of phosphorus-solubilized, nitrogenase activity, 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase activity, production of indole-3-acetic acid (IAA), secretion of iron carrier and so on. Finally, the screening multifunctional phosphorus-dissolving bacteria were identified, which were combined with colony characteristics, physiological and biochemical tests and molecular biotechnology. Results: (1) Thirteen phosphorus-dissolving bacteria were isolated and screened in total, and P5 (195.61 mg·L−1) had the strongest capacity of phosphorus-solubilized. Five phosphorus-dissolving bacteria were provided with nitrogenase activity, and the highest activity of nitrogenase was P10 and P5 (71.90 C2H4 nmol·mL−1·h−1 and 71.00 C2H4 nmol·mL−1·h−1, respectively). Four strains were provided with ACC deaminase activity, and the highest activity of ACC deaminase was P5 and P9, (0.74 μmol·mg−1·h−1 and 0.54 μmol·mg−1·h−1, respectively). Most strains could secrete IAA, and three strains of bacteria had a strong secretory ability, which could secrete IAA with a concentration greater than 15 mg·mL−1, and P5 was 18.00, P2 was 17.30, P6 was 15.59 (mg·mL−1). P5 produced carriers of iron better than others, and the ratio of the diameter of the iron production carrier ring to the diameter of the colony was 1.80, respectively, which was significantly higher than other strains. Combining all kinds of factors, P5 multifunctional phosphorus-dissolving bacteria were screened for eventual further study. (2) Strain P5 was identified as Burkholderia ubonensis, based on the colony characteristics, physiological and biochemical tests, 16SrDNA sequence analysis and phylogenetic tree construction. Conclusion: P5 has a variety of high-efficiency growth-promoting capabilities, and the ability to produce IAA, ACC deaminase activity and siderophore performance are significantly higher than other strains, which had great potential in the development of microbial fertilizer.

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