scholarly journals Accelerated gastric ulcer healing in thyroxine-treated rats: roles of gastric acid, mucus, and inflammatory response

2018 ◽  
Vol 96 (6) ◽  
pp. 597-602 ◽  
Author(s):  
Olasupo S. Adeniyi ◽  
Benjamin O. Emikpe ◽  
Samuel B. Olaleye
Keyword(s):  
Gastric Ulcer ◽  
Acid Secretion ◽  
Gastric Acid ◽  
Ulcer Healing ◽  
Gastric Mucus ◽  
Gastric Ulcer Healing ◽  
Neutrophil Lymphocyte Ratio ◽  
Ulcer Area ◽  
Thyroxine Treatment ◽  
Male Wistar Rats

The roles of gastric acid, mucus, and inflammation on the pro-ulcer-healing effect of thyroid hormone were investigated. Male Wistar rats were randomly divided into four groups: control, thyroidectomised, thyroidectomised with thyroxine treatment (100 μg·kg–1·day–1), and sham-operated animals treated with thyroxine. Thirty-five days after thyroidectomy, sham surgery, or thyroxine treatment, an ulcer was experimentally induced. Healing was assessed 3, 7, and 10 days post-ulceration by measurement of the ulcer area, gastric mucus and acid secretion, and neutrophil lymphocyte ratio (NLR) as an index of inflammation. By day 10, the ulcer area had decreased in all groups. Recovery was significantly greater (P < 0.05) in thyroxine-treated rats (78.5% ± 1.6% reduction in ulcer area) than in controls (72.3% ± 1.2% reduction) or thyroidectomised rats (63.3% ± 1.9% reduction). Thyroxine-treated animals also had the highest reduction in NLR (65.0% ± 2.5%). Mucus secretion was significantly lower (P < 0.05) in thyroidectomised rats by days 3 and 7. Furthermore, by day 10, the concentration of basal acid decreased by 77.4% ± 2.6% in thyroxine-treated, 65.0% ± 0.0% in control, and 51.5% ± 3.3% in thyroidectomised rats. We conclude that thyroxine accelerates gastric ulcer healing by altering mucus and acid secretion and reducing NLR.

Role of cyclooxygenase-2 in modulating gastric acid secretion in the normal and inflamed rat stomach

2000 ◽  
Vol 279 (6) ◽  
pp. G1292-G1297 ◽  
Author(s):  
Kirsty Barnett ◽  
Cameron J. Bell ◽  
Webb McKnight ◽  
Michael Dicay ◽  
Keith A. Sharkey ◽  
...  
Keyword(s):  
Gastric Ulcer ◽  
Acid Secretion ◽  
Gastric Acid Secretion ◽  
Gastric Acid ◽  
Ulcer Healing ◽  
Cyclooxygenase 2 ◽  
Gastric Ulcer Healing ◽  
Cyclooxygenase 1 ◽  
Basal Acid ◽  
Inflammatory Drugs

Nonsteroidal anti-inflammatory drugs elevate gastric acid secretion, possibly contributing to their ability to interfere with gastric ulcer healing. Inhibitors of cyclooxygenase-2 have been shown to delay experimental gastric ulcer healing. In the present study, we tested the hypothesis that cyclooxygenase-2-derived prostaglandins modulate gastric acid secretion. Studies were performed in normal rats and in rats with iodoacetamide-induced gastritis. Inflammation in the latter group was confirmed histologically and by a threefold increase in tissue levels of the granulocyte marker myeloperoxidase and was also associated with overexpression of cyclooxygenase-2 in the stomach. Basal acid secretion in both groups of rats was not affected by pretreatment with DuP-697, a selective inhibitor of cyclooxygenase-2. A nonselective cyclooxygenase inhibitor, indomethacin, had no effect on acid secretion in normal rats but caused a doubling of acid secretion in the rats with gastritis. DuP-697 had no effect on pentagastrin-induced secretion in either group of rats. Gastritis itself was associated with significantly increased pentagastrin-induced acid secretion, and this was further increased in rats pretreated with indomethacin. These results suggest that in a setting of gastric inflammation, prostaglandins derived from cyclooxygenase-1, not cyclooxygenase-2, exert inhibitory effects on acid secretion.

scholarly journals Chrysophyllum Albidum Accelerates Delayed Gastric Ulcer Healing in Rats Through Oxidative Stress Reversal and Proton Pump Inhibition

2021 ◽  
Vol 16 (2) ◽  
pp. 163-175
Author(s):  
A.T. Salami ◽  
A. D. Famurewa ◽  
T. P Omayone ◽  
T. F. Iyiola ◽  
S. B. Olaleye
Keyword(s):  
Gastric Ulcer ◽  
Proton Pump ◽  
Ulcer Healing ◽  
Positive Control ◽  
Negative Control ◽  
Gastric Ulcer Healing ◽  
Proton Pump Inhibition ◽  
Male Wistar Rats ◽  
Underlying Mechanisms

Background: Chrysophyllum albidum has been documented to exert its gastric ulcer (GU) healing activities by modulating blood inflammatory mediators, however, other probable in-vivo underlying mechanisms are still vague which this study sought to investigate.Materials and Methods: Male Wistar rats (120-130g) divided into 9 groups (n=15 for groups I-VII; n=5 for groups VIII & IX) viz: Groups I- positive control (DUnA); II and III–250 and 500mg/kg methanolic extract of C. albidum (MeCaB) bark respectively; IV, V and VI-100mg/kg fractions A, B and C respectively; VII–30mg/kg omeprazole; VIII-ulcerated untreated (baseline), IX-negative control. Chronic GU was induced experimentally and delayed using indomethacin with 14 days simultaneous drug treatment. Gastric ulcer score, mucin content, antioxidant and proton pump activities were evaluated by days 3, 7 and 14 of treatment. Data were expressed as Mean+SEM and P<0.05 was significant.Results: C. albidum and fractions treated groups significantly decreased gastric ulcer scores and lipid peroxidation compared with DUnA. Negative control, C. albidum and fraction treated groups significantly increased superoxide dismutase, catalase, glutathione levels and mucin content compared with DUnA group by days 3 and 7. C. albidum, Negative and baseline control groups significantly decreased H+K+ATPase activities compared with DUnA by day14.Conclusion: C. albidum and its fractions facilitated the healing of gastric ulcer, probably by enhanced antioxidant levels, mucin content and decreased gastric H+K+ATPase activity. Keywords: C. albidum and chromatographic fractions, gastric ulcer healing, mucin , antioxidant, H+/K+ATPase pump.

The Therapeutic Effect of Nigella sativa Seed on Indomethacin-induced Gastric Ulcer in Rats

2020 ◽  
Vol 16 (3) ◽  
pp. 276-283
Author(s):  
Maryam Paseban ◽  
Saeed Niazmand ◽  
Mohammad Soukhtanloo ◽  
Naser T. Meibodi ◽  
Abbasali Abbasnezhad ◽  
...  
Keyword(s):  
Gastric Ulcer ◽  
Acid Secretion ◽  
Protein Content ◽  
Gastric Acid Secretion ◽  
Gastric Acid ◽  
Therapeutic Effect ◽  
Nigella Sativa ◽  
Gastric Mucus ◽  
Ulcer Index ◽  
Nigella Sativa Seed

Background: Nonsteroidal anti-inflammatory drugs (NSAIDs) are widely used to relieve pain and reduce inflammation. However, gastric complications remain a major problem limiting their clinical usage. This study was carried out to evaluate the therapeutic effect of Nigella sativa seed (N. sativa seed) hydroalcoholic extract on indomethacin-induced gastric ulcer in rats and its possible mechanism. Methods: This study was carried out on forty-eight male Wistar rats. Gastric ulcer was induced by indomethacin (35 mg/kg). N. sativa seed extract (100, 200, and 400 mg/kg) and ranitidine (50 mg/kg) was administered orally for five days after ulcer induction. Ulcer index, gastric acid secretion, gastric mucus content, total thiol, malondialdehyde (MDA), and total hexose, and protein content in gastric juice were determined. Results: The ulcer index in groups of N. sativa seed was significantly lower as compared to indomethacin group. N. sativa seed significantly decreased MDA and protein content, but increased total thiol, total hexose, and mucus content as compared to indomethacin group. N. sativa seed did not affect gastric acid secretion. Conclusion: These findings showed that the gastroprotective effect of N. sativa seed against indomethacin- induced ulcer was mainly exerted by antioxidant activity, stimulation of gastric mucus secretion and also increased total hexose in the gastric mucosa.

Effects of artemisinin, with or without lumefantrine and amodiaquine on gastric ulcer healing in rat

2018 ◽  
Vol 29 (5) ◽  
pp. 515-524 ◽  
Author(s):  
Kazeem O. Ajeigbe ◽  
Benjamin O. Emikpe ◽  
Samuel Babafemi Olaleye
Keyword(s):  
Gastric Ulcer ◽  
Ulcer Healing ◽  
Neutrophil Infiltration ◽  
Mucosal Injury ◽  
Total Leukocyte Count ◽  
Control Group ◽  
Albino Rats ◽  
Gastric Ulcer Healing ◽  
Neutrophil Lymphocyte Ratio ◽  
Reactive Protein

Abstract Background Antimalarial drugs have been shown to predispose the stomach to ulceration in rats. However, their role in the modulation of gastric ulcer healing is not known. The aim of the present study is to investigate the effect of artemisinin-based combination therapies on ulcer healing. Methods Gastric kissing ulcers were induced in 40 male albino rats (150–180 g) using 0.2 mL 50% acetic acid. One day after the ulcer induction, experimental rats were divided into four groups and treated once daily orally for 3 days as follows: (1) normal saline, (2) artemether-lumefantrine (2/12 mg/kg), (3) artesunate-amodiaquine (4/10 mg/kg), and (4) artesunate (2 mg/kg) only. A fifth group of 10 rats served as overall control with no ulcer induced and no treatment given. Ulcer healing was determined on days 4 and 7 post induction using ulcer score and planimetry. Results Artesunate decreased ulcer severity by 12.5% and 52.0% on days 4 and 7, respectively. Significant increases in severity were observed in rats treated with artemether-lumefantrine (25.0% and 40.0%) and artesunate-amodiaquine (50.0% and 95.0%). Lipid peroxidation was decreased by artesunate by day 7 (27%; p<0.05) but increased in artemether-lumefantrine and artesunate-amodiaquine administered rats (63.6% and 55%; p<0.05). The activity of superoxide dismutase was reduced by artesunate-amodiaquine on day 7 (22%; p<0.05) but no effect in the artemether-lumefantrine treatment. Neutrophil infiltration, total leukocyte count, neutrophil-lymphocyte ratio, and C-reactive protein values were significantly increased in the artemether-lumefantrine and artesunate-amodiaquine treated groups when compared with the untreated ulcer control group (p<0.05). These variables were all reduced by artesunate (p<0.05). Conclusions This study revealed that although artesunate may be beneficial in gastric ulcer healing, its combination with either lumefantrine or amodiaquine may delay healing of gastric mucosal injury.

scholarly journals The preventive effect of Nigella sativa seed on gastric ulcer induced by indomethacin in rat

2020 ◽  
Vol 9 (1) ◽  
pp. 12-19
Author(s):  
Maryam Paseban ◽  
Saeed Niazmand ◽  
Mohammad Soukhtanloo ◽  
Naser Tayyebi Meybodi
Keyword(s):  
Gastric Ulcer ◽  
Acid Secretion ◽  
Protein Content ◽  
Gastric Acid Secretion ◽  
Gastric Acid ◽  
Nigella Sativa ◽  
Gastric Ulceration ◽  
Gastric Mucus ◽  
Ulcer Index ◽  
Nigella Sativa Seed

Introduction: Nonsteroidal anti-inflammatory drugs (NSAIDs) are considered as one of the most administrated groups of medications worldwide. Due to the role of NSAIDs in inducing gastric ulceration, their clinical applications are still challenging. Nigella sativa seed is widely used as an herbal medication against gastrointestinal complications. The present experiment was carried out to investigate the impact of N. sativa seed hydro-alcoholic extract on gastric ulcer induced by indomethacin (IND) and to evaluate its possible mechanisms in rat. Methods: This study was performed on 48 male Wistar rats. Acute gastric ulceration was induced by IND (35 mg/kg). N. sativa seed extract (100, 200, 400 mg/kg) and ranitidine (50 mg/kg) were administered orally for five days before the induction ulcer. Ulcer index, gastric acid secretion, gastric mucus content, glutathione (GSH), malondialdehyde (MDA), total hexose, gastric juice protein content were determined on the fifth day. Results: The ulcer index in all groups of N. sativa seed was significantly lower than that of the IND group. N sativa seed considerably decreased MDA and protein content, but increased total thiol, total hexose, and mucus content compared to the IND group. N. sativa seed did not affect gastric acid secretion. Conclusion: These findings were indicative of the gastroprotective effect of N. sativa seed against the IND-induced ulcer, suggesting that it can mainly be exerted through the anti-oxidant activity of the extract as well as its role in stimulating gastric mucus secretion and increasing total hexose in the gastric mucosa.

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