A Preliminary Ploidy Analysis of Diploid and Triploid Salmonids

1994 ◽  
Vol 51 (S1) ◽  
pp. 38-41 ◽  
Author(s):  
R. L. Teplitz ◽  
J. E. Joyce ◽  
S. I. Doroshov ◽  
B. H. Min
Keyword(s):  
Nuclear Dna ◽  
Computer Assisted ◽  
Image Analyzer ◽  
Cell Analysis ◽  
Dna Quantitation ◽  
Histologic Evaluation ◽  
Computer Assisted Image ◽  
The University ◽  
Kidney Liver ◽  
National Marine Fisheries Service

The findings indicate that mosaicism does occur in triploids and especially in triploid hybrids. Immature diploid and triploid salmon were obtained from the University of Alaska – National Marine Fisheries Service. These were fixed in 10% buffered formalin and blocks taken from various tissues including kidney, liver, spleen, and gonads. The paraffin blocks were sectioned at 4 μm and stained with hematoxylin and eosin for histologic evaluation and to quantitate nuclear DNA. The slides for DNA evaluation were stained using the Cell Analysis Systems, Inc. (CAS, Elmhurst, IL) kit following Feulgen hydrolysis. The Cell Analysis Systems, Inc. (Model 200) computer-assisted image analyzer was used for DNA quantitation. An attempt was made to evaluate the frequency of mosaicism, ploidy variation in somatic and gonadal tissues of triploids and hybrids as compared with normal diploids. Hepatic ploidy variation, normal in adult animals, was not present in these specimens. However, in gonads there was evidence of increased mosaicism among the triploids. Because of mosaicism in gonadal tissue, sterility of triploids cannot be presumed with certainty at this stage. Further studies are planned to examine this and other questions raised by these results.

Hemodynamic and histomorphometric characteristics of dilated cardiomyopathy of Syrian hamsters (Bio TO-2 strain)

2001 ◽  
Vol 79 (4) ◽  
pp. 329-337 ◽  
Author(s):  
Sonia Goineau ◽  
Danielle Pape ◽  
Pascal Guillo ◽  
Marie-Paule Ramée ◽  
Eric Bellissant
Keyword(s):  
Blood Flow ◽  
Regional Blood Flow ◽  
Computer Assisted ◽  
Image Analyzer ◽  
Hepatic Congestion ◽  
Femoral Blood Flow ◽  
Cardiomyopathic Hamsters ◽  
History Of ◽  
Computer Assisted Image ◽  
Femoral Blood

The natural history of the disease of the dilated strain Bio TO-2 of cardiomyopathic hamsters (CMH) is not totally characterized. We investigated its hemodynamic and histomorphometric characteristics at 140, 180, 220, 260, and 300 days of age. Forty CMH and 40 controls were investigated (8 at each stage). Mean arterial pressure (MAP, carotid artery catheter) and cardiac output and femoral blood flow (CO, FBF, transit time method) were measured in anesthetized animals. Systemic (SVR) and femoral (FVR) vascular resistances were calculated. Atria, left and right ventricles (LV, RV), lungs, and liver were weighed. LV cavity area, LV and RV wall thicknesses and collagen densities were determined (computer-assisted image analyzer). Pulmonary and hepatic congestion were assessed (arbitrary scales). Compared with controls, MAP, CO and FBF were significantly lower in CMH throughout the study (on average: –22%, –34%, –33%, respectively), FVR was significantly increased (+15%), but SVR was not significantly modified. Concerning histomorphometric characteristics, differences between groups significantly increased with age for most variables: at 300 days, atria (+292%), RV (+13%), lungs (+44%), and liver (+23%) weights, LV cavity area (+130%), LV (+364%) and RV (+181%) collagen densities were significantly increased in CMH vs controls, whereas LV (–40%) and RV (–23%) wall thicknesses were significantly decreased. At 260 and 300 days, CMH showed significant pulmonary congestion without hepatic alteration. Bio TO-2 CMH progressively develop an alteration of cardiac function leading to decreased MAP and musculo-cutaneous blood flow associated with cardiac remodeling including atria hypertrophy and LV dilation, wall thinning and a rise in collagen density.Key words: cardiomyopathy, heart failure, regional blood flow, remodeling.

scholarly journals DNA IMAGE CYTOMETRY IN PROGNOSTICATION OF COLORECTAL CANCER: PRACTICAL CONSIDERATIONS OF THE TECHNIQUE AND INTERPRETATION OF THE HISTOGRAMS

2011 ◽  
Vol 25 (1) ◽  
pp. 1 ◽  
Author(s):  
Abdelbaset Buhmeida ◽  
Yrjo Collan ◽  
Kari Syrjanen ◽  
Seppo Pyrhonen
Keyword(s):  
Colorectal Cancer ◽  
Nuclear Dna ◽  
Image Cytometry ◽  
Computer Assisted ◽  
Dna Values ◽  
Computer Assisted Image ◽  
Analytical Approaches ◽  
Dna Image Cytometry ◽  
Dna Histograms

The role of DNA content as a prognostic factor in colorectal cancer (CRC) is highly controversial. Some of these controversies are due to purely technical reasons, e.g. variable practices in interpreting the DNA histograms, which is problematic particularly in advanced cases. In this report, we give a detailed account on various options how these histograms could be optimally interpreted, with the idea of establishing the potential value of DNA image cytometry in prognosis and in selection of proper treatment. Material consists of nuclei isolated from 50 ƒĘm paraffin sections from 160 patients with stage II, III or IV CRC diagnosed, treated and followed-up in our clinic. The nuclei were stained with the Feulgen stain. Nuclear DNA was measured using computer-assisted image cytometry. We applied 4 different approaches to analyse the DNA histograms: 1) appearance of the histogram (ABCDE approach), 2) range of DNA values, 3) peak evaluation, and 4) events present at high DNA values. Intra-observer reproducibility of these four histogram interpretation was 89%, 95%, 96%, and 100%, respectively. We depicted selected histograms to illustrate the four analytical approaches in cases with different stages of CRC, with variable disease outcome. In our analysis, the range of DNA values was the best prognosticator, i.e., the tumours with the widest histograms had the most ominous prognosis. These data implicate that DNA cytometry based on isolated nuclei is valuable in predicting the prognosis of CRC. Different interpretation techniques differed in their reproducibility, but the method showing the best prognostic value also had high reproducibility in our analysis.

Application of a computer assisted image analyzer to the assessment of area and number of sites of dental attrition and its use for age estimation

1991 ◽  
Vol 50 (1) ◽  
pp. 97-109 ◽  
Author(s):  
Takeshi Kambe ◽  
Kosei Yonemitsu ◽  
Kazuhiko Kibayashi ◽  
Shigeyuki Tsunenari
Keyword(s):  
Age Estimation ◽  
Computer Assisted ◽  
Image Analyzer ◽  
Dental Attrition ◽  
Computer Assisted Image

scholarly journals Comparing a Computer-Assisted Image Analyzer to a Pathologist in the Scoring of Ki-67 Breast Cancer Images

2013 ◽  
Vol 140 (suppl 1) ◽  
pp. A231-A231
Author(s):  
Caleb Cheng ◽  
Amanda Stephenson ◽  
Suman Setty ◽  
Bruce Levy ◽  
Elizabeth Wiley
Keyword(s):  
Breast Cancer ◽  
Computer Assisted ◽  
Image Analyzer ◽  
Ki 67 ◽  
Computer Assisted Image

scholarly journals Quantitative analysis of whole body autoradiogram using a computer-assisted image analyzer.

1988 ◽  
Vol 3 (2) ◽  
pp. 121-135 ◽  
Author(s):  
Shin-ichiro Nagatsuka ◽  
Shin-ya Hanawa ◽  
Takashi Honda ◽  
Masaru Hasegawa
Keyword(s):  
Quantitative Analysis ◽  
Whole Body ◽  
Computer Assisted ◽  
Image Analyzer ◽  
Computer Assisted Image

Computer Assisted Image Reconstruction of Oligomer Structure

1976 ◽  
Vol 34 ◽  
pp. 472-473
Author(s):  
A.M. Jones ◽  
A. Max Fiskin
Keyword(s):  
Three Dimensional ◽  
Depth Of Field ◽  
Computer Assisted ◽  
Projection Data ◽  
Two Dimensional ◽  
Single Particles ◽  
Dimensional Reconstruction ◽  
Computer Assisted Image ◽  
The Fourier Transform ◽  
Space Approach

If the tilt of a specimen can be varied either by the strategy of observing identical particles orientated randomly or by use of a eucentric goniometer stage, three dimensional reconstruction procedures are available (l). If the specimens, such as small protein aggregates, lack periodicity, direct space methods compete favorably in ease of implementation with reconstruction by the Fourier (transform) space approach (2). Regardless of method, reconstruction is possible because useful specimen thicknesses are always much less than the depth of field in an electron microscope. Thus electron images record the amount of stain in columns of the object normal to the recording plates. For single particles, practical considerations dictate that the specimen be tilted precisely about a single axis. In so doing a reconstructed image is achieved serially from two-dimensional sections which in turn are generated by a series of back-to-front lines of projection data.

New polarized-light microscope for fast and orientation independent measurement of birefringent fine structure

1993 ◽  
Vol 51 ◽  
pp. 82-83
Author(s):  
Rudolf Oldenbourg
Keyword(s):  
Light Microscope ◽  
Polarized Light ◽  
Video Camera ◽  
Limited Range ◽  
Image Point ◽  
Computer Assisted ◽  
Optical Modulators ◽  
Anisotropic Structures ◽  
Long Time ◽  
Computer Assisted Image

The polarized light microscope has the unique potential to measure submicroscopic molecular arrangements dynamically and non-destructively in living cells and other specimens. With the traditional pol-scope, however, single images display only those anisotropic structures that have a limited range of orientations with respect to the polarization axes of the microscope. Furthermore, rapid measurements are restricted to a single image point or single area that exhibits uniform birefringence or other form of optical anisotropy, while measurements comparing several image points take an inordinately long time.We are developing a new kind of polarized light microscope which combines speed and high resolution in its measurement of the specimen anisotropy, irrespective of its orientation. The design of the new pol-scope is based on the traditional polarized light microscope with two essential modifications: circular polarizers replace linear polarizers and two electro-optical modulators replace the traditional compensator. A video camera and computer assisted image analysis provide measurements of specimen anisotropy in rapid succession for all points of the image comprising the field of view.

Teaching Computer Based Historical Data Analysis

1994 ◽  
Vol 6 (1) ◽  
pp. 52-58 ◽  
Author(s):  
Charles Anderson ◽  
Robert J. Morris
Keyword(s):  
Data Analysis ◽  
Social History ◽  
Historical Data ◽  
Good Practice ◽  
Computer Assisted ◽  
Historical Data Analysis ◽  
University Of Edinburgh ◽  
Computer Based ◽  
The University

A case study ofa third year course in the Department of Economic and Social History in the University of Edinburgh isusedto considerandhighlightaspects of good practice in the teaching of computer-assisted historical data analysis.

Computer-Assisted Language Learning at the University of Dundee

2013 ◽  
Vol 1 (3) ◽  
pp. 10-12
Author(s):  
Derek R. Lewis
Keyword(s):  
Language Learning ◽  
Computer Assisted ◽  
Computer Assisted Language Learning ◽  
The University
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