scholarly journals DNA IMAGE CYTOMETRY IN PROGNOSTICATION OF COLORECTAL CANCER: PRACTICAL CONSIDERATIONS OF THE TECHNIQUE AND INTERPRETATION OF THE HISTOGRAMS

2011 ◽  
Vol 25 (1) ◽  
pp. 1 ◽  
Author(s):  
Abdelbaset Buhmeida ◽  
Yrjo Collan ◽  
Kari Syrjanen ◽  
Seppo Pyrhonen
Keyword(s):  
Colorectal Cancer ◽  
Nuclear Dna ◽  
Image Cytometry ◽  
Computer Assisted ◽  
Dna Values ◽  
Computer Assisted Image ◽  
Analytical Approaches ◽  
Dna Image Cytometry ◽  
Dna Histograms

The role of DNA content as a prognostic factor in colorectal cancer (CRC) is highly controversial. Some of these controversies are due to purely technical reasons, e.g. variable practices in interpreting the DNA histograms, which is problematic particularly in advanced cases. In this report, we give a detailed account on various options how these histograms could be optimally interpreted, with the idea of establishing the potential value of DNA image cytometry in prognosis and in selection of proper treatment. Material consists of nuclei isolated from 50 ƒĘm paraffin sections from 160 patients with stage II, III or IV CRC diagnosed, treated and followed-up in our clinic. The nuclei were stained with the Feulgen stain. Nuclear DNA was measured using computer-assisted image cytometry. We applied 4 different approaches to analyse the DNA histograms: 1) appearance of the histogram (ABCDE approach), 2) range of DNA values, 3) peak evaluation, and 4) events present at high DNA values. Intra-observer reproducibility of these four histogram interpretation was 89%, 95%, 96%, and 100%, respectively. We depicted selected histograms to illustrate the four analytical approaches in cases with different stages of CRC, with variable disease outcome. In our analysis, the range of DNA values was the best prognosticator, i.e., the tumours with the widest histograms had the most ominous prognosis. These data implicate that DNA cytometry based on isolated nuclei is valuable in predicting the prognosis of CRC. Different interpretation techniques differed in their reproducibility, but the method showing the best prognostic value also had high reproducibility in our analysis.

DNA Image cytometry as a prognostic tool in stage II and stage III colorectal cancer

2006 ◽  
Vol 24 (18_suppl) ◽  
pp. 13565-13565
Author(s):  
A. Buhmeida ◽  
A. Ålgars ◽  
R. Ristamäki ◽  
Y. Collan ◽  
K. Syrjänen ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Nuclear Dna Content ◽  
Image Cytometry ◽  
Stage Ii ◽  
Stage Iii ◽  
Computer Assisted ◽  
Primary Tumors ◽  
Free Survival ◽  
Wide Range ◽  
Dna Image Cytometry

13565 Background: We assessed the prognostic value of nuclear DNA content measured in the primary tumors of 123 patients with stage II or stage III colorectal cancer (CRC). Methods: Isolated nuclei from paraffin sections were stained with Feulgen and DNA was measured using a computer-assisted image analysis cytometry system (Ahrens ACAS). We applied 4 different approaches in analysis of DNA histograms: ABCDE approach, histogram range, peak evaluation, and DNA cut-off values. Results: Using the histogram range, narrow range was rare (3.7%) in patients who died of disease as compared with 16.4% among those alive (p=0.017). Modal peak evaluation was a significant predictor of disease free survival (DFS) (Kaplan-Meier log-rank p=0.0235). In the range evaluation, the first set (low-start gates) was a significant predictor of DFS (log-rank p=0.0121), where disease recurrence was closely associated the widest range (1.8c->10c) gates. Recurrence-free survival was markedly better among patients with narrow gate histograms than wide range histograms than among patients with wide range histograms (p<0.03). The first set also proved to be significant predictor of disease specific survival (DSS) (log-rank p=0.0045), being markedly better (78–90.0%) among the patients with the narrow-gate histograms. Grading of the histogram range into two categories (with 6.0c as cut-off for low and wide range), was a powerful predictor of both DSS (log-rank p= 0.0092) and 5-year DFS (p=0.0106) in the whole series, and separately in Stage III (but not Stage II) disease; p=0.0131 and p=0.0201, respectively. Conclusions: The DNA image cytometry with careful analysis of the histograms may provide valuable prognostic information in CRC, with potential clinical implications in patient management, particularly in predicting the patients at high risk for recurrence who should be considered as candidates for adjuvant therapy. No significant financial relationships to disclose.

scholarly journals The prognostic significance of determining DNA content in breast cancer by DNA image cytometry: the role of high grade aneuploidy in node negative breast cancer

2007 ◽  
Vol 60 (6) ◽  
pp. 649-655 ◽  
Author(s):  
S. Yildirim-Assaf ◽  
A. Coumbos ◽  
W. Hopfenmuller ◽  
H.-D. Foss ◽  
H. Stein ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Dna Content ◽  
Prognostic Significance ◽  
Image Cytometry ◽  
High Grade ◽  
Node Negative ◽  
Node Negative Breast Cancer ◽  
Dna Image Cytometry

scholarly journals The impact of digital imaging in the field of cytopathology

CytoJournal ◽  
2009 ◽  
Vol 6 ◽  
pp. 6 ◽  
Author(s):  
Liron Pantanowitz ◽  
Maryanne Hornish ◽  
Robert A. Goulart
Keyword(s):  
Digital Imaging ◽  
Pap Test ◽  
Image Cytometry ◽  
Virtual Microscopy ◽  
Diagnostic Techniques ◽  
Computer Assisted ◽  
Training And Education ◽  
Automated Screening ◽  
The Impact

With the introduction of digital imaging, pathology is undergoing a digital transformation. In the field of cytology, digital images are being used for telecytology, automated screening of Pap test slides, training and education (e.g. online digital atlases), and proficiency testing. To date, there has been no systematic review on the impact of digital imaging on the practice of cytopathology. This article critically addresses the emerging role of computer-assisted screening and the application of digital imaging to the field of cytology, including telecytology, virtual microscopy, and the impact of online cytology resources. The role of novel diagnostic techniques like image cytometry is also reviewed.

A Preliminary Ploidy Analysis of Diploid and Triploid Salmonids

1994 ◽  
Vol 51 (S1) ◽  
pp. 38-41 ◽  
Author(s):  
R. L. Teplitz ◽  
J. E. Joyce ◽  
S. I. Doroshov ◽  
B. H. Min
Keyword(s):  
Nuclear Dna ◽  
Computer Assisted ◽  
Image Analyzer ◽  
Cell Analysis ◽  
Dna Quantitation ◽  
Histologic Evaluation ◽  
Computer Assisted Image ◽  
The University ◽  
Kidney Liver ◽  
National Marine Fisheries Service

The findings indicate that mosaicism does occur in triploids and especially in triploid hybrids. Immature diploid and triploid salmon were obtained from the University of Alaska – National Marine Fisheries Service. These were fixed in 10% buffered formalin and blocks taken from various tissues including kidney, liver, spleen, and gonads. The paraffin blocks were sectioned at 4 μm and stained with hematoxylin and eosin for histologic evaluation and to quantitate nuclear DNA. The slides for DNA evaluation were stained using the Cell Analysis Systems, Inc. (CAS, Elmhurst, IL) kit following Feulgen hydrolysis. The Cell Analysis Systems, Inc. (Model 200) computer-assisted image analyzer was used for DNA quantitation. An attempt was made to evaluate the frequency of mosaicism, ploidy variation in somatic and gonadal tissues of triploids and hybrids as compared with normal diploids. Hepatic ploidy variation, normal in adult animals, was not present in these specimens. However, in gonads there was evidence of increased mosaicism among the triploids. Because of mosaicism in gonadal tissue, sterility of triploids cannot be presumed with certainty at this stage. Further studies are planned to examine this and other questions raised by these results.

The Role of Computer-Assisted Image-Guided Techniques

2001 ◽  
Vol 12 (02) ◽  
pp. 175-182 ◽  
Author(s):  
Jaimie M. Henderson
Keyword(s):  
Computer Assisted ◽  
Image Guided ◽  
Computer Assisted Image

scholarly journals A New Protocol of Computer-Assisted Image Analysis Highlights the Presence of Hemocytes in the Regenerating Cephalic Tentacles of Adult Pomacea canaliculata

2021 ◽  
Vol 22 (9) ◽  
pp. 5023
Author(s):  
Giulia Bergamini ◽  
Mohamad Ahmad ◽  
Marina Cocchi ◽  
Davide Malagoli
Keyword(s):  
Image Analysis ◽  
Freshwater Snail ◽  
Biological Information ◽  
Pomacea Canaliculata ◽  
Computer Assisted ◽  
Specific Cell ◽  
Organ Regeneration ◽  
Computer Assisted Image ◽  
Basic Studies

In humans, injuries and diseases can result in irreversible tissue or organ loss. This well-known fact has prompted several basic studies on organisms capable of adult regeneration, such as amphibians, bony fish, and invertebrates. These studies have provided important biological information and helped to develop regenerative medicine therapies, but important gaps concerning the regulation of tissue and organ regeneration remain to be elucidated. To this aim, new models for studying regenerative biology could prove helpful. Here, the description of the cephalic tentacle regeneration in the adult of the freshwater snail Pomacea canaliculata is presented. In this invasive mollusk, the whole tentacle is reconstructed within 3 months. Regenerating epithelial, connective, muscular and neural components are already recognizable 72 h post-amputation (hpa). Only in the early phases of regeneration, several hemocytes are retrieved in the forming blastema. In view of quantifying the hemocytes retrieved in regenerating organs, granular hemocytes present in the tentacle blastema at 12 hpa were counted, with a new and specific computer-assisted image analysis protocol. Since it can be applied in absence of specific cell markers and after a common hematoxylin-eosin staining, this protocol could prove helpful to evidence and count the hemocytes interspersed among regenerating tissues, helping to unveil the role of immune-related cells in sensory organ regeneration.

Computer Assisted Cervical Cytology

2009 ◽  
pp. 160-184 ◽  
Author(s):  
Liron Pantanowitz
Keyword(s):  
Information Management ◽  
Information Technologies ◽  
Pap Test ◽  
Image Cytometry ◽  
Control Measures ◽  
Cervical Cytology ◽  
Computer Assisted ◽  
Successful Operation ◽  
Information Management Systems

Automation and emerging information technologies are being adopted by cytology laboratories around the world to augment Pap test screening and improve diagnostic accuracy. Informatics, the application of computers and information systems to information management, is therefore essential for the successful operation of the cytopathology laboratory. This chapter describes how laboratory information management systems can be used to achieve an automated and seamless workflow process. The utilization of software, electronic databases and spreadsheets to perform necessary quality control measures will be discussed. The emerging role of computer assisted screening and application of digital imaging to the field of cervical cytology will be described, including telecytology and virtual microscopy. Finally, this chapter will reflect on the impact of online cytology resources and the emerging role of digital image cytometry.

The role of DNA image cytometry in screening oral potentially malignant lesions using brushings: A systematic review

Oral Oncology ◽  
2019 ◽  
Vol 96 ◽  
pp. 51-59 ◽  
Author(s):  
Madhurima Datta ◽  
Denise Laronde ◽  
Branko Palcic ◽  
Martial Guillaud
Keyword(s):  
Systematic Review ◽  
Image Cytometry ◽  
Potentially Malignant ◽  
Malignant Lesions ◽  
Dna Image Cytometry

scholarly journals DNA Ploidy and Chromosomal Imbalances in Invasive Ductal Breast Cancer. A Comparative Study of DNA Image Cytometry and Comparative Genomic Hybridization (CGH)

2000 ◽  
Vol 20 (2-3) ◽  
pp. 69-82 ◽  
Author(s):  
Katrin Friedrich ◽  
Jens Scheithauer ◽  
Volker Dimmer ◽  
Wolfdietrich Meyer ◽  
Franz Theissig ◽  
...  
Keyword(s):  
Genetic Background ◽  
Dna Ploidy ◽  
Image Cytometry ◽  
Comparative Genomic ◽  
Breast Cancers ◽  
Chromosomal Imbalances ◽  
Invasive Ductal Breast Cancer ◽  
Ductal Breast Cancer ◽  
Dna Image Cytometry

Chromosomal imbalances were analyzed in 62 breast cancers with different DNA ploidy by CGH. The results of DNA image cytometry and CGH are consistent with peridiploid and aneuploid cases. The peritetraploid tumors harbored a high number of chromosomal imbalances, as a hint for an unfavorable prognosis. The quantitative analysis of imbalances highlighted the role of different physical constituents of the chromosome, and of chromosomal losses in different DNA ploidy groups. The peritetraploid and aneuploid tumors differed from the peridiploid tumors in losses at 8p and 18q. The peritetraploid cancers exhibited more gains at 8q, the aneuploid tumors more losses at 17p than their peridiploid counterparts. The aneuploid cases differed from the peritetraploid tumors in a higher number of losses at 11q and 14q. Combinations of imbalances provide further insights into the genetic background of DNA ploidy. Hypotheses for the progression from peridiploid to nondiploid breast cancers are given.Figures onhttp://www.esacp.org/acp/2000/20-2_3/friedrich.htm.

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