ADDITIONAL OBSERVATIONS ON THE PREPARATION OF R BANDED HUMAN CHROMOSOMES WITH ACRIDINE ORANGE

1976 ◽  
Vol 18 (1) ◽  
pp. 45-50 ◽  
Author(s):  
Ram S. Verma ◽  
Herbert A. Lubs
Keyword(s):  
Acetic Acid ◽  
Acridine Orange ◽  
Careful Attention ◽  
Human Chromosomes ◽  
Ethanol Acetic Acid ◽  
Technical Factors

A number of technical factors which affect acridine orange R banding (RFA banding) were studied. These variables included age of slide, timing of fixation, details of incubation, mounting and the use of sequential technics. Optimal RFA banding was obtained between 15 and 20 days but good or very good preparations were obtained between 7 days and 2 months. Improved results were obtained in slides that were 3–4 months old by refixing the slides in ethanol acetic acid. Intermittent movement of slides during incubation in buffer as well as the details of mounting and removal of cover slips were found to be important. The best sequential banding was obtained with the sequence of Q to R but good results were obtained with the sequence G to R using ASG banding. Satisfactory results with the sequence R to C were not obtained. With careful attention to these variables good RFA banding can be obtained over a period of several months.

scholarly journals Fabrication of TiO2/Carbon Photocatalyst using Submerged DC Arc Discharged in Ethanol/Acetic Acid Medium

2017 ◽  
Vol 202 ◽  
pp. 012058 ◽  
Author(s):  
T E Saraswati ◽  
A O Nandika ◽  
I F Andhika ◽  
Patiha ◽  
C Purnawan ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Acid Medium ◽  
Acetic Acid Medium ◽  
Ethanol Acetic Acid ◽  
Dc Arc

Characteristics of Piperine Solubility in Multiple Solvent

2011 ◽  
Vol 236-238 ◽  
pp. 2495-2498 ◽  
Author(s):  
Xue Song Huang ◽  
Xian Zhe Lin ◽  
Mo Ting Guo ◽  
Ya Zou
Keyword(s):  
Experimental Data ◽  
High Performance Liquid Chromatography ◽  
Acetic Acid ◽  
High Performance ◽  
Authentic Sample ◽  
Allometric Model ◽  
Experiment Data ◽  
Reverse Phase ◽  
Ethanol Acetic Acid ◽  
Acetic Acid Water

The solution of piperine in multiple solvent including ethanol, acetic acid, water and HCl were investigated to extract more piperine from piper fruit. Piperine was determined by reverse phase high-performance liquid chromatography with Diamonsil column (C18,5 μm ,250 mm×4. 6 mm) at 343 nm. Experiment data were simulated by Allometric model and the formula is Z=0.9+ 4.54×10-10×x5.675+1.8029×y2.12848+2.37×10-10×x5.675×y2.12848(Z:sample solution,mol/mL,x: the percentage of ethanol’s volume, ml/100mL,y: the acetic acid in the authentic sample solution, g/100mL), the adj·R2=0.997, the comparative deviation less than 2%. These results are good in agreement with experimental data. It reveals that the model can meet the requirements of the selection and design in extracting piperine from piper fruit.

THE ANAEROBIC DISSIMILATION OF D-GLUCOSE-1-C14, D-ARABINOSE-1-C14, AND L-ARABINOSE-1-C14 BY AEROBACTER AEROGENES

1954 ◽  
Vol 32 (1) ◽  
pp. 147-153 ◽  
Author(s):  
A. C. Neish ◽  
F. J. Simpson
Keyword(s):  
Carbon Dioxide ◽  
Acetic Acid ◽  
Lactic Acid ◽  
Carboxyl Groups ◽  
Methyl Groups ◽  
Carboxyl Group ◽  
Complete Conversion ◽  
Aerobacter Aerogenes ◽  
Methyl Group ◽  
Ethanol Acetic Acid

D-Glucose-1-C14, D-arabinose-1-C14, and L-arabinose-1-C14 were dissimilated anaerobically by Aerobacter aerogenes. The major products (2,3-butanediol, ethanol, acetic acid, lactic acid, formic acid, and carbon dioxide) were isolated and the location of C14 determined. The products from glucose were all labeled, mainly in the methyl groups, in agreement with the hypothesis that they were derived from methyl-labeled pyruvate formed by the reactions of the classical Embden–Meyerhof scheme for glycolysis. The products from both pentoses appeared to have been formed from pyruvate labeled in both the methyl and carboxyl groups with twice as much C14 in the methyl group as in the carboxyl group. This result may be explained quantitatively by a hypothesis assuming complete conversion of pentose to triose via a heptulose.

Synthesen mit aliphatischen Dialdehyden, XXVII [1] Eine „non-template“-Synthese zur Darstellung metallfreier 1.4.8.11-Tetraaza [14] annulen-Derivate / Syntheses with Aliphatic Dialdehydes, XXVII [1] A Non-Template Synthesis for the Preparation of Metal-Free 1,4,8,11-Tetraaza[14]annulene Derivatives

1978 ◽  
Vol 33 (9) ◽  
pp. 1012-1015 ◽  
Author(s):  
Christian Reichardt ◽  
Wolfgang Scheibelein
Keyword(s):  
Acetic Acid ◽  
Template Synthesis ◽  
Mass Spectra ◽  
Metal Cations ◽  
Metal Free ◽  
Ethanol Acetic Acid ◽  
Chelate Ligands

The reaction of 2-substituted malonaldehydes (7) with aromatic 1,2-diamines (5 or 6) in ethanol/acetic acid (10:1) leads to 6,13-disubstituted 1,4,8,11 -tetraaza[14]annulene derivatives (8 or 9) even in the absence of coordinating metal cations ("non-template" synthesis), and not to 3-substituted 1,5-benzodiazepines such as 4. Cobalt(II), nickel(II), and copper(II) complexes of the macrocyclic chelate ligands 8 and 9 have been observed by subsequent metallization with the corresponding acetates in N,N-dimethylformamide. The structures of 8-11 have been assigned mainly by their mass spectra.

scholarly journals THYMIDINE DEGRADATION PRODUCTS IN PLANT TISSUES LABELED WITH TRITIATED THYMIDINE

1963 ◽  
Vol 17 (1) ◽  
pp. 59-66 ◽  
Author(s):  
S. T. Takats ◽  
R. M. S. Smellie
Keyword(s):  
Acetic Acid ◽  
Metabolic Pathways ◽  
Time Course ◽  
Degradation Products ◽  
Tritiated Thymidine ◽  
Lilium Longiflorum ◽  
Plant Tissues ◽  
Root Tips ◽  
Radioactivity Analysis ◽  
Ethanol Acetic Acid

A study of the metabolic pathways of H3-thymidine utilization in buds of Lilium longiflorum and root tips of Vicia faba was undertaken in order to obtain information that might explain the binding of H3 from H3-thymidine in the cytoplasm of these plants. H3-thymidine was administered for various periods of time, the tissues were fixed and processed in the manner routinely used in preparation for sectioning and autoradiography, and the radioactivity removed in this way from the tissues was determined. It was found that the ethanol/acetic acid fixative contained the major portion of the radioactivity. Analysis of this extract by paper chromatography showed that the radioactivity was distributed among various degradation products of thymidine, principally ß-ureidoisobutyric acid and ß-aminoisobutyric acid. Time course experiments with Vicia showed that these degradation products rapidly appeared in the tissue during incubation with H3-thymidine, while H3-thymine appeared in the incubation medium. Preliminary studies indicated that Vicia root tips incubated with H3-dihydrothymine for 24 hours would bind a small amount of H3 non-specifically in the cells. It seems unlikely that utilization of degradation products of H3-thymidine is sufficient to explain labeling which is concentrated in the cytoplasm.

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