Genetic relatedness among rabbiteye blueberry (Vaccinium ashei) cultivars determined by DNA amplification using single primers of arbitrary sequence

Genome ◽  
1993 ◽  
Vol 36 (5) ◽  
pp. 971-977 ◽  
Author(s):  
Myneni Aruna ◽  
Peggy Ozias-Akins ◽  
Max E. Austin ◽  
Gary Kochert
Keyword(s):  
Polymerase Chain Reaction ◽  
Genetic Distance ◽  
Genetic Relatedness ◽  
Random Amplified Polymorphic Dna ◽  
Dna Amplification ◽  
Arbitrary Sequence ◽  
Chain Reaction ◽  
Vaccinium Ashei ◽  
Polymerase Chain ◽  
Improved Cultivars

Most improved cultivars of commercially important hexaploid rabbiteye blueberry were developed from only four native selections collected from the wild; thus, many cultivars are closely related by lineage. The consanguinity among major cultivars is a potential problem, as the rabbiteye blueberries are highly self-incompatible natural outcrossers with potential inbreeding depression. We investigated the extent of genetic relatedness among 15 improved cultivars and four wild selections by the technique of random amplified polymorphic DNA, also referred to as arbitrarily primed polymerase chain reaction. Single decanucleotides of arbitrary sequence revealed polymorphism among cultivars and wild selections. Genetic distances were estimated based on the amount of band sharing. Cluster analysis of genetic distance estimates tended to group siblings with each other and with one or both parents. The average genetic distance between improved cultivars decreased relative to the four wild parental selections, which might indicate progression towards inbreeding. The significance of increased genetic relatedness among the improved cultivars of rabbiteye blueberry and the application of molecular methods in breeding and commercial cultivation is discussed.Key words: genetic distance, polymerase chain reaction, inbreeding.

Genetic segregation of random amplified polymorphic DNA in diploid cultivated alfalfa

Genome ◽  
1992 ◽  
Vol 35 (1) ◽  
pp. 84-87 ◽  
Author(s):  
C. S. Echt ◽  
L. A. Erdahl ◽  
T. J. McCoy
Keyword(s):  
Polymerase Chain Reaction ◽  
Rapd Markers ◽  
Genome Mapping ◽  
Polymerase Chain Reaction Amplification ◽  
Random Amplified Polymorphic Dna ◽  
Rapid Development ◽  
Arbitrary Sequence ◽  
Chain Reaction ◽  
Backcross Population ◽  
Polymerase Chain

Polymerase chain reaction was used, with single 10-mer primers of arbitrary sequence, to amplify random regions of genomic DNA from a diploid cultivated alfalfa backcross population. Segregation of the random amplified polymorphic DNA (RAPD) fragments was analysed to determine if RAPD markers are suitable for use as genetic markers. Of the 19 primers tested, 13 amplified a total of 37 polymorphic fragments, of which 28 (76%) segregated as dominant Mendelian traits. RAPD markers appear useful for the rapid development of genetic information in species like alfalfa where little information currently exists or is difficult to obtain.Key words: random amplified polymorphic DNA, polymerase chain reaction amplification, genome mapping, restriction fragment length polymorphism, Medicago sativa.

scholarly journals Randomly Amplified Polymorphic DNA Fingerprinting for Identifying Rabbiteye Blueberry (Vaccinium ashei Reade) Cultivars

1995 ◽  
Vol 120 (5) ◽  
pp. 710-713 ◽  
Author(s):  
Myneni Aruna ◽  
Max E. Austin ◽  
Peggy Ozias-Akins
Keyword(s):  
Molecular Weight ◽  
Polymerase Chain Reaction ◽  
Dna Fingerprinting ◽  
Genomic Dna ◽  
Genetic Constitution ◽  
Arbitrary Sequence ◽  
Randomly Amplified Polymorphic Dna ◽  
Chain Reaction ◽  
Vaccinium Ashei ◽  
Polymerase Chain

Cultivars of the economically important rabbiteye blueberry (Vaccinium ashei Reade) were differentiated at the DNA level using the technique of randomly amplified polymorphic DNA. Single decanucleotide primers of arbitrary sequence were used to amplify genomic DNA by the polymerase chain reaction. All cultivars tested exhibited a unique set of collective amplified fragments of distinct molecular weight. A blind fingerprinting experiment resulted in identification of unknown samples without ambiguity. We also clarified the genetic identity of two wild selections of rabbiteye blueberry, `Ethel' and `Satilla', which have been maintained as two different selections, hut are considered by some blueberry breeders to be of the same genetic constitution. The technique also verified the probable identity of two cultivars in a commercial blueberry field by comparing their amplified DNA patterns with those of standard cultivars. No variation was observed between the amplification profiles of `Brightwell' and its presumed sport. A cultivar key based on 11 markers amplified by four primers is presented.

Genetic Relatedness and Variability in Three Marine Angelfish Species Revealed by Arbitrarily Primed Polymerase Chain Reaction

2011 ◽  
Vol 1 (6) ◽  
pp. 244-247
Author(s):  
Manish Kumar ◽  
M. Thangaraj ◽  
T. T. Ajith Kumar ◽  
R. Thirumaraiselvi
Keyword(s):  
Polymerase Chain Reaction ◽  
Genetic Distance ◽  
Related Species ◽  
Genetic Relatedness ◽  
Distinct Species ◽  
Species Status ◽  
Chain Reaction ◽  
Closely Related Species ◽  
Random Primers ◽  
Polymerase Chain

The arbitrarily primed polymerase chain reaction was used to estimate thegenetic relationships and variability in three closely related species of angelfishes(Family Pomacanthidae). Ten random primers were screened to identifyspecies â€specific bands in Pomacanthus imperator, P. annularis and Apolemichthysxanthurus. The total numbers of scorable bands were 253, inwhich the numbers of species  â€specific bands were 153. By this study it wasobserved that, P. imperator was very closer to P. annularis with a geneticsimilarity of 0.1322and farthest to A. xanthurus with the genetic distance of0.8182. The UPGMA â€ neighbour joining tree grouped the three species intoseparate clusters emphasizing the distinct species status.

PCR BY THERMAL CONVECTION

2004 ◽  
Vol 18 (16) ◽  
pp. 775-784 ◽  
Author(s):  
DIETER BRAUN
Keyword(s):  
Polymerase Chain Reaction ◽  
Thermal Convection ◽  
Dna Amplification ◽  
Reaction Vessel ◽  
Cold Region ◽  
Chain Reaction ◽  
Heating And Cooling ◽  
Highly Sensitive ◽  
Specific Amplification ◽  
Polymerase Chain

The Polymerase Chain Reaction (PCR) allows for highly sensitive and specific amplification of DNA. It is the backbone of many genetic experiments and tests. Recently, three labs independently uncovered a novel and simple way to perform a PCR reaction. Instead of repetitive heating and cooling, a temperature gradient across the reaction vessel drives thermal convection. By convection, the reaction liquid circulates between hot and cold regions of the chamber. The convection triggers DNA amplification as the DNA melts into two single strands in the hot region and replicates into twice the amount in the cold region. The amplification progresses exponentially as the convection moves on. We review the characteristics of the different approaches and show the benefits and prospects of the method.

Genetic diversity and phylogeny analysis of Azolla based on DNA amplification by arbitrary primers

Genome ◽  
1993 ◽  
Vol 36 (4) ◽  
pp. 686-693 ◽  
Author(s):  
Benoit Van Coppenolle ◽  
Iwao Watanabe ◽  
Charles Van Hove ◽  
Gerard Second ◽  
Ning Huang ◽  
...  
Keyword(s):  
Principal Component Analysis ◽  
Polymerase Chain Reaction ◽  
Principal Component ◽  
Dna Amplification ◽  
Component Analysis ◽  
Genetic Distances ◽  
Group Method ◽  
Chain Reaction ◽  
Arbitrary Primers ◽  
Polymerase Chain

The polymerase chain reaction was used to amplify random sequences of DNA from 25 accessions of Azolla to evaluate the usefulness of this technique for identification and phylogenetic analysis of this aquatic fern. Accessions were selected to represent all known species within the genus Azolla and to encompass the worldwide distribution of the fern. Primers of 10 nucleotides with 70% G + C content were used to generate randomly amplified polymorphic DNA from the symbiotic Azolla–Anabaena complex. Twenty-two primers were used and each primer gave 4–10 bands of different molecular weights for each accession. Bands were scored as present or absent for each accession and variation among accessions was quantified using Nei's genetic distances. A dendrogram summarizing phenetic relationships among the 25 accessions was generated using the unweighted pair-group method with arithmetic mean. Principal component analysis was also used to evaluate genetic similarities. Three distinct groups were identified: group 1 contains five species, group 2 contains the pinnata species, and group 3 contains the nilotica species. The analysis demonstrates that the major groups of Azolla species can be easily distinguished from one an other and, in addition, that closely related accessions within species can be identified. We further found that using 10 primers, a phylogeny that is essentially the same as that derived from 22 primers can be constructed. Our results suggest that total DNA extracted from the Azolla–Anabaena symbionts is useful for classification and phylogenetic studies of Azolla.Key words: Azolla–Anabaena symbiosis, genetic distances, polymerase chain reaction, principal component analysis.

scholarly journals HTLV-I-induced lymphoma mimicking Hodgkin's disease. Diagnosis by polymerase chain reaction amplification of specific HTLV-I sequences in tumor DNA

Blood ◽  
1988 ◽  
Vol 71 (4) ◽  
pp. 1027-1032 ◽  
Author(s):  
DB Duggan ◽  
GD Ehrlich ◽  
FP Davey ◽  
S Kwok ◽  
J Sninsky ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Hodgkin's Disease ◽  
Polymerase Chain Reaction Amplification ◽  
Hodgkin’S Disease ◽  
Dna Amplification ◽  
Disease Diagnosis ◽  
Lymphoproliferative Disease ◽  
Enzyme Linked Immunosorbent Assay ◽  
Chain Reaction ◽  
Polymerase Chain

Abstract A patient with a localized HTLV-I-associated lymphoproliferative disease that was misdiagnosed as Hodgkin's disease is presented. The patient's serum was negative for HTLV-I antibodies by enzyme-linked immunosorbent assay (ELISA), Western blot, and radioimmunoprecipitation. Tumor tissue DNA was negative for HTLV-I by Southern blotting but was positive for distinct HTLV-I sequences when subjected to DNA amplification using the polymerase chain reaction. We conclude that the clinical and pathologic diagnosis of HTLV-I-related lymphoma can be difficult and can be confused with Hodgkin's disease. Extremely sensitive molecular biological techniques may be required to establish a diagnosis of HTLV-I-induced lymphoma.

Novel DNA amplification on chromosomes 2p25.3 and 7q11.23 in cholangiocarcinoma identified by arbitrarily primed polymerase chain reaction

2005 ◽  
Vol 131 (12) ◽  
pp. 821-828 ◽  
Author(s):  
S. Chariyalertsak ◽  
T. Khuhaprema ◽  
V. Bhudisawasdi ◽  
B. Sripa ◽  
S. Wongkham ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Dna Amplification ◽  
Chain Reaction ◽  
Polymerase Chain
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