A NEW PROCEDURE FOR THE DETECTION AND ENUMERATION OF COAGULASE-POSITIVE STAPHYLOCOCCI FROM FROZEN SEAFOODS

1966 ◽  
Vol 12 (1) ◽  
pp. 191-198 ◽  
Author(s):  
H. Raj
Keyword(s):  
Liquid Medium ◽  
Solid Medium ◽  
Sorbic Acid ◽  
Egg Yolk ◽  
Confirmatory Test ◽  
Precipitation Reaction ◽  
Large Numbers ◽  
Step Procedure ◽  
Coagulase Positive Staphylococci ◽  
Coagulase Positive Staphylococcus

A two-step procedure in which a new anaerobic liquid medium is used for a presumptive test and a solid medium (staphylococcus medium No, 110 fortified with egg yolk) incubated at 45 °C for a confirmatory test has been developed for the detection and enumeration of coagulase-positive staphylococci in frozen seafoods. The presumptive liquid medium called "Mannitol Salt Sorbic acid broth" provides specific enrichment to coagulase-positive staphylococci even in the presence of large numbers (104–105 cells) of mixed flora common to these foods. The addition of seafood material to the above medium does not affect its selective performance or its sensitivity. Also, the medium is equally efficient in recovering very low numbers of coagulase-positive staphylococcus subjected to freezing, even in the presence of the mixed flora. Incubation at 45 °C in the confirmatory step not only gives an accelerated egg yolk precipitation reaction typical of coagulase-positive staphylococci, but also enhances the specificity of the procedure.

Un milieu gélosé pour le dénombrement direct de Staphylococcus aureus : milieu au plasma de porc pour S. aureus (PPSA)

1976 ◽  
Vol 22 (11) ◽  
pp. 1603-1611 ◽  
Author(s):  
J-J. Devoyod ◽  
Liliane Millet ◽  
G. Mocquot
Keyword(s):  
Staphylococcus Aureus ◽  
Dairy Products ◽  
Agar Medium ◽  
Egg Yolk ◽  
Plasma Medium ◽  
Large Numbers ◽  
Selective Agar ◽  
Coagulase Positive Staphylococci

A selective agar medium (pork plasma medium for S. aureus (PPSA)) enables the direct enumeration of coagulase-positive staphylococci. This medium is based on the Baird-Parker agar without egg yolk and is supplemented with pig plasma. Colonies of Staphylococcus aureus are surrounded by a halo of precipitated fibrin. When foods such as dairy products contain large numbers of egg yolk – negative strains of S. aureus, the PPSA agar has the advantage over egg yolk containing media such as Baird-Parker agar that fewer suspect colonies have to be confirmed.

scholarly journals A Novel 6-Benzyl Ether Benzoxaborole Is Active against Mycobacterium tuberculosisIn Vitro

2017 ◽  
Vol 61 (9) ◽  
Author(s):  
Nipul Patel ◽  
Theresa O'Malley ◽  
Yong-Kang Zhang ◽  
Yi Xia ◽  
Bjorn Sunde ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Liquid Medium ◽  
Inhibitory Concentration ◽  
Solid Medium ◽  
Eukaryotic Cells ◽  
Intracellular Bacteria ◽  
Benzyl Ether ◽  
Content Type ◽  
Resistant Mutants

ABSTRACT We identified a novel 6-benzyl ether benzoxaborole with potent activity against Mycobacterium tuberculosis. The compound had an MIC of 2 μM in liquid medium. The compound was also able to prevent growth on solid medium at 0.8 μM and was active against intracellular bacteria (50% inhibitory concentration [IC50] = 3.6 μM) without cytotoxicity against eukaryotic cells (IC50 > 100 μM). We isolated resistant mutants (MIC ≥ 100 μM), which had mutations in Rv1683, Rv3068c, and Rv0047c.

Influence of Surfactants on Biodegradation of Chlordane by Wood-Rotting Fungus

2013 ◽  
Vol 361-363 ◽  
pp. 908-911
Author(s):  
Peng Fei Xiao ◽  
Tie Xue You ◽  
Yu Zhen Song ◽  
Shan Ying ◽  
Jian Qiao Wang
Keyword(s):  
Liquid Medium ◽  
Degradation Rate ◽  
Solid Medium ◽  
Fungal Growth ◽  
Tween 80 ◽  
Positive Effects ◽  
Lower Effect ◽  
Triton X

Wood-rotting fungus,Phlebia lindtneriGB 1027, was tested in toxicity assays with three surfactants in order to select surfactants for degradation assays of chlordane. Tween 80 and Triton X-100 appeared to have lower effect on the fungal growth on solid medium, while higher effect of fungal growth was observed in solid medium with SDS. Tween 80 had positive effects both on the chlordane degradation and the fungal growth. When fungus was incubated on PDB liquid medium with Tween 80 of 10 CMC after 20 d, 78.6% of chlordane was removed. In the treatments with Triton X-100, this strain showed comparatively greatest degradation rate (70.8%) of chlordane at a concentration of 2 CMC. However, when Triton X-100 concentration was higher than 2 CMC (5 and 10 CMC), the enhancement for the biodegradation of chlordane decreased.

scholarly journals Egg Yolk Reaction of Coagulase-Positive Staphylococci on Egg Yolk Agar Medium

1977 ◽  
Vol 30 (1) ◽  
pp. 31-36
Author(s):  
TADATAKA OKUBO ◽  
T. ODA ◽  
Y. TANAKA
Keyword(s):  
Agar Medium ◽  
Egg Yolk ◽  
Coagulase Positive Staphylococci

Use of Iron Milk Medium for Enumeration of Clostridium perfringens

1982 ◽  
Vol 65 (5) ◽  
pp. 1129-1133 ◽  
Author(s):  
William D St John ◽  
Jack R Matches ◽  
Marleen M Wekell
Keyword(s):  
Clostridium Perfringens ◽  
Incubation Time ◽  
Incubation Temperature ◽  
Egg Yolk ◽  
Most Probable Number ◽  
Biochemical Tests ◽  
Probable Number ◽  
Large Numbers ◽  
Whole Milk ◽  
Short Incubation Time

Abstract A simple iron milk medium was used for isolation and enumeration of Clostridium perfringens from soil, sludge, and water samples. The whole milk contained only iron powder as a reducing agent; no other inhibitors were added. The iron milk most probable number (MPN) procedure was compared with 4 plating media: sulfite-polymyxin-sulfadiazine, Shahidi-Ferguson perfringens, tryptose-sulfite- cycloserine (both with and without egg yolk), and tryptone-sulfite-neomycin. The selectivity of the iron milk relies solely on the rapid growth of C. perfringens at 45°C and the stormy fermentation reaction within 18 h. Isolates were confirmed as C. perfringens by standard biochemical tests. The iron milk MPN procedure compared very well with the 4 plating media tested. Selectivity of incubation temperature, short incubation time, and ease of identification by the characteristic stormy fermentation make this method ideal for enumerating C. perfringens from large numbers of samples.

The Survival of Mycoplasma suipneumoniae in Liquid Medium, on Solid Medium and in Pneumonic Tissue

1972 ◽  
Vol 13 (2) ◽  
pp. 203-204 ◽  
Author(s):  
R.F.W. Goodwin
Keyword(s):  
Liquid Medium ◽  
Solid Medium

scholarly journals INFECTION OF MICE WITH MAMMALIAN TUBERCLE BACILLI GROWN IN TWEEN-ALBUMIN LIQUID MEDIUM

1947 ◽  
Vol 86 (2) ◽  
pp. 159-174 ◽  
Author(s):  
Cynthia Pierce ◽  
René J. Dubos ◽  
Gardner Middlebrook
Keyword(s):  
Liquid Medium ◽  
Brain Tissue ◽  
Egg Yolk ◽  
Tuberculous Infection ◽  
Infectious Process ◽  
Intraperitoneal Route ◽  
Rate Of Progression ◽  
Different Strains ◽  
Intracerebral Infection ◽  
The Brain

Introduction of the bacilli by the mtravenous route or by feeding gives rise to a disease predominantly localized in the lungs. Following intracerebral infection, the bacilli first multiply rapidly in the brain tissue, and then invade other organs, producing lesions especially in the lungs. Injection of the bacilli by the intraperitoneal route is less effective than by either the intravenous or intracerebral routes; however, admixture of the bacilli with some of the components of egg yolk increases both the infectivity and the pulmonary localization. Different strains of mice differ markedly in their susceptibility to experimental tuberculous infection; the highest susceptibility was observed among the pigmented strains (line 1 dba and C57 black). Greater resistance does not appear to depend on the ability to prevent the establishment of infection, but rather corresponds to a slower rate of progression of the infectious process. It is possible to produce in mice tuberculosis presenting any desired degree of acuteness or chronicity by controlling certain factors which condition the initiation and the progression of the infection.

OCCURRENCE OF BACILLUS CEREUS IN SELECTED DRY FOOD PRODUCTS1,2

1971 ◽  
Vol 34 (1) ◽  
pp. 12-15 ◽  
Author(s):  
H. U. Kim ◽  
J. M. Goepfert
Keyword(s):  
Bacillus Cereus ◽  
Egg Yolk ◽  
The United States ◽  
Food Samples ◽  
Confirmatory Test ◽  
Foodborne Disease ◽  
Biochemical Tests ◽  
Precipitin Test ◽  
Dry Food

One hundred seventy samples of dried food products in national distribution were examined for the incidence and level of contamination by Bacillus cereus. Twenty-five per cent of the samples yielded B. cereus at a level not exceeding 4000 per gram. Mannitol-egg yolk-polymyxin (MYP) agar was used as a presumptive test for the presence of B. cereus in the food samples. Various biochemical tests for the confirmation of suspicious colonies appearing on MYP agar were evaluated. A precipitin test employing spore precipitinogens was investigated as a confirmatory test for B. cereus. The possible role of B. cereus in outbreaks of foodborne disease in the United States is discussed.

MICROBIOLOGY OF WYOMING BIG GAME MEAT1

1974 ◽  
Vol 37 (3) ◽  
pp. 129-131 ◽  
Author(s):  
F. C. Smith ◽  
R. A. Field ◽  
J. C. Adams
Keyword(s):  
Mule Deer ◽  
Fecal Coliforms ◽  
Ground Meat ◽  
Salmonella Spp ◽  
Game Meat ◽  
Aging Period ◽  
Large Numbers ◽  
Plate Counts ◽  
Coagulase Positive Staphylococci ◽  
Total Bacteria

Total bacteria present on antelope, mule deer, and elk carcasses and in ground meat from these carcasses were determined. In addition to total aerobic plate counts, counts for coliforms, fecal coliforms, coagulase-positive staphylococci, and Salmonella spp. were made. Average total aerobic plate counts of ground game ranged from 0.7 to 53 million organisms per gram. Surface swabs showed the bacterial counts to increase greatly during the 2-week aging period. Large numbers of coliforms and fecal coliforms were found in game meat. Nevertheless, game meat contained very few coagulase-positive staphylococci and no Salmonella spp. were detected.

PHENOTYPIC CHARACTERIZATION OF BENECKEA PARAHAEMOLYTICA: A PRELIMINARY REPORT1

1973 ◽  
Vol 36 (4) ◽  
pp. 214-219 ◽  
Author(s):  
Paul Baumann ◽  
Linda Baumann
Keyword(s):  
Liquid Medium ◽  
Type Strain ◽  
Solid Medium ◽  
Morphological Characterization ◽  
The Other ◽  
Polar Flagellum ◽  
Phenotypic Characterization ◽  
Phenotypic Traits ◽  
General Properties

Eighty-six strains which were isolated from cases of gastroenteritis and had the general properties of the genus Beneckea were submitted to an extensive nutritional, physiological, and morphological characterization. The results indicated that this collection of strains, which included the type strain of Beneckea parahaemolytica, was phenotypically homogeneous and distinguishable from the other known species of Beneckea by multiple, unrelated, phenotypic traits. When grown in liquid medium, strains of B. parahaemolytica had single, sheathed, polar flagella; when grown on solid medium, these strains had unsheathed, peritrichous flagella in addition to the sheathed, polar flagellum. Additional traits of use for differentiation of this species from the remaining species of the genus Beneckea were the ability of B. parahaemolytica to grow at 40 C, utilize d-galactose, l-leucine, l-histidine, and putrescine and the inability to utilize sucrose, dl-β-hydroxy-butyrate or give a positive Voges-Proskauer reaction. The validity of some of the traits previously used to identify B. parahaemolytica as well as the possible difficulties encountered in the identification of this organism from marine sources are considered.

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