A simple plate test for the determination of protocatechuic acid utilization by soil bacteria

1968 ◽  
Vol 14 (1) ◽  
pp. 88-89 ◽  
Author(s):  
Veronica Sundman
Keyword(s):  
Soil Bacteria ◽  
Chloride Solution ◽  
Protocatechuic Acid ◽  
Ferric Chloride Solution ◽  
Soil Extract ◽  
Bacterial Isolates ◽  
Ferric Ions ◽  
Plate Test ◽  
Soil Extract Agar

The method described makes use of the color reaction between protocatechuic acid and ferric ions. Separately sterilized protocatechuic acid is added to soil extract agar before plates are poured. After suitable incubation the plates are flooded with ferric chloride solution. Colorless zones around growth contrasting with the green color of the protocatechuic acid containing agar indicate utilization of protocatechuic acid. With the aid of this method the frequency of strains that use protocatechuic acid, among random bacterial isolates of soil origin, was found to vary between 26 and 44% according to the soil investigated.

scholarly journals Determination of free acid in ferric chloride solution (reivestigation of JIS K 1447).

1992 ◽  
Vol 41 (3) ◽  
pp. T45-T47 ◽  
Author(s):  
Masanobu SAHARA ◽  
Hirotoshi YOSHIMURA ◽  
Fumio SAGARA ◽  
Keihei UFAO ◽  
Isao YOSHIDA ◽  
...  
Keyword(s):  
Ferric Chloride ◽  
Chloride Solution ◽  
Free Acid ◽  
Ferric Chloride Solution

scholarly journals Determination of free acid in ferric chloride solution.

1968 ◽  
Vol 17 (10) ◽  
pp. 1273-1278 ◽  
Author(s):  
Masayoshi EZAWA ◽  
Osamu MATSUZAKI
Keyword(s):  
Ferric Chloride ◽  
Chloride Solution ◽  
Free Acid ◽  
Ferric Chloride Solution

New colorimetric method for quantitative determination of protein in urine.

1977 ◽  
Vol 23 (5) ◽  
pp. 811-812 ◽  
Author(s):  
H Yatzidis
Keyword(s):  
Quantitative Determination ◽  
Tannic Acid ◽  
Chloride Solution ◽  
Room Temperature ◽  
Colorimetric Method ◽  
Ferric Chloride Solution ◽  
Acid Protein ◽  
Analytical Recovery ◽  
Violet Color

Abstract Total urinary protein is rapidly precipitated at room temperature by tannic acid. The tannic acid/protein precipitate, dissolved in aqueous triethanolamine/ferric chloride solution, gives a purple-violet color of high absorptivity. Absorbance at 510 nm is linearly related to concentration from 0.05 to 1.50 A for a protein content of 0.05 to 1.50 g/liter, and less than 5 mg/liter can be detected. The CV and analytical recovery ranged from 0.5 to 1.8% and 98 to 103%, respectively. Nonprotein urinary constituents do not interfere.

scholarly journals Improved Nuclear Staining of Asparagus Microspores for Cytological Analysis

HortScience ◽  
1997 ◽  
Vol 32 (4) ◽  
pp. 735-736 ◽  
Author(s):  
Asma Ziauddin ◽  
Mingsheng Peng ◽  
David J. Wolyn
Keyword(s):  
Developmental Stage ◽  
Ferric Chloride ◽  
Chloride Solution ◽  
Ferric Chloride Solution ◽  
Asparagus Officinalis ◽  
Nuclear Staining ◽  
Cytological Analysis ◽  
Distinct Nucleus ◽  
Generative Nuclei

Clear visualization of asparagus (Asparagus officinalis L.) microspore nuclei with common stains such as acetocarmine or DAPI is difficult, hindering cytological analyses. The addition of saturated aqueous ferric chloride solution to Carnoy's I fixative (30 μL·mL-1) resulted in clear visualization of nuclei. A distinct nucleus was observed in uninucleate cells and the vegetative and generative nuclei were clearly visible in binucleate microspores. This method can be used reliably for determination of asparagus microspore developmental stage. Chemical name used: 4′,6-diamidino-2-phenylindole-2HCL (DAPI).

scholarly journals Increased culturability of soil bacteria from Marcellus shale temperate forest in Pennsylvania

2013 ◽  
Vol 2 (1) ◽  
pp. 36-42 ◽  
Author(s):  
Evan Mitchell Lutton ◽  
Rosa Schellevis ◽  
Anupama Shanmuganathan
Keyword(s):  
16S Rdna ◽  
Soil Bacteria ◽  
Temperate Forest ◽  
Culture Medium ◽  
Culture Media ◽  
Marcellus Shale ◽  
Soil Extract ◽  
Extract Agar ◽  
Unique Otus ◽  
Soil Extract Agar

Soil bacteria comprise a largely untapped resource with only 1-10% of bacterial species predicted to live in soil being culturable in the laboratory. Establishing culture-dependent protocols that identify unique operational taxonomic units (OTUs) is an important research topic in soil bacterial ecology. The culturability of soil bacteria may be improved by employing different culture media due to inherent preferences of growth substrate utilization. Soil-extract agar, R-2A agar, and 1% nutrient agar were used in this study to isolate bacteria obtained from soil samples collected in winter months to increase the understanding of bacterial diversity in Abernathy Field Station, a Marcellus shale temperate forest in Washington, Pennsylvania. Changes in bacterial diversity can be used to assess the early impact of anthropological factors, such as hydraulic fracturing in the Marcellus shale region, which may lead to severe environmental problems. For the purpose of long term ecological monitoring, data obtained from this year’s sample collection were analyzed in conjunction with previous years’ assessments. Bacterial isolates were analyzed taxonomically and phylogenetically. Unique OTUs were identified through comparative analysis of 16S rDNA. The Shannon-Weaver and Simpson’s diversity indices ranked isolates on soil-extract agar highest for species richness, and rarefaction analysis suggests that sampling saturation of OTUs identified on soil-extract agar has not yet been reached. Each culture medium studied supported isolates of four common phyla: Actinobacteria, Bacteroidetes, Firmicutes, and Proteobacteria. Soil-extract agar supported the greatest proportion of pigmented colonies including a Cyanobacterium which exhibited intra-16S rDNA polymorphism. Each culture medium supported the growth of unique OTUs and genera with Bacillus, Flavobacterium, Pseudomonas, Rhizobium, and Streptomyces found on each. This study suggests that utilizing different culture media can increase the culturability of soil bacteria.

scholarly journals Spectrophotometric Determination of Bisphenol A by Complexation with Ferricyanide and Ferric chloride solution

2007 ◽  
Vol 17 (2) ◽  
pp. 266-271 ◽  
Author(s):  
Eun-Joo Kum ◽  
Hee-Young Ryu ◽  
Gi-Seok Kwon ◽  
Ho-Yong Sohn
Keyword(s):  
Bisphenol A ◽  
Spectrophotometric Determination ◽  
Ferric Chloride ◽  
Chloride Solution ◽  
Ferric Chloride Solution

Biodegradation of organophosphorus Pollutants by Soil Bacteria: Biochemical Aspects and Unsolved Problems

2020 ◽  
Vol 36 (4) ◽  
pp. 126-135
Author(s):  
T.V. Shushkova ◽  
D.O. Epiktetov ◽  
S.V. Tarlachkov ◽  
I.T. Ermakova ◽  
A.A. Leontievskii
Keyword(s):  
Genome Sequencing ◽  
Soil Bacteria ◽  
Activity Regulation ◽  
Bacterial Isolates ◽  
Bacterial Strains ◽  
Russian Science ◽  
Degrading Bacteria ◽  
Phosphorus Source ◽  
Glyphosate Herbicide ◽  
Enzymatic Pathways

The degradation of persistent organophosphorus pollutants have been studied in 6 soil bacterial isolates and in 3 bacterial strains adapted for utilization of glyphosate herbicide (GP) under laboratory conditions. Significant differences in the uptake of organophosphonates were found in taxonomically close strains possessing similar enzymatic pathways of catabolism of these compounds, which indicates the existence of unknown mechanisms of activity regulation of these enzymes. The effect of adaptation for GP utilization as a sole phosphorus source on assimilation rates of several other phosphonates was observed in studied bacteria. The newly found efficient stains provided up to 56% of GP decomposition after application to the soil in the laboratory. The unresolved problems of microbial GP metabolism and the trends for further research on the creation of reliable biologicals capable of decomposing organophosphonates in the environment are discussed. organophosphonates, glyphosate, biodegradation, bioremediation, C-P lyase, phosphonatase, degrading bacteria Investigation of phosphonatase and genome sequencing were supported by Russian Science Foundation Grant no. 18-074-00021.

scholarly journals Dual-emission ratio fluorescence for selective and sensitive detection of ferric ions and ascorbic acid based on one-pot synthesis of glutathione protected gold nanoclusters

RSC Advances ◽  
2021 ◽  
Vol 11 (28) ◽  
pp. 17283-17290
Author(s):  
Shuai Zhang ◽  
Cong Zhang ◽  
Xiaodong Shao ◽  
Rentian Guan ◽  
Yingying Hu ◽  
...  
Keyword(s):  
Ascorbic Acid ◽  
Gold Nanoclusters ◽  
Sensitive Detection ◽  
Dual Fluorescence ◽  
Ferric Ions ◽  
Fluorometric Method ◽  
One Pot ◽  
Dual Emission ◽  
One Pot Synthesis

A fluorometric method was proposed for the determination of Fe3+ and ascorbic acid (AA) based on blue and red dual fluorescence emissions of glutathione (GSH) stabilized-gold nanoclusters (AuNCs).

Leaching and kinetic modelling of low-grade calcareous sphalerite in acidic ferric chloride solution

2009 ◽  
Vol 96 (4) ◽  
pp. 275-282 ◽  
Author(s):  
R. Dehghan ◽  
M. Noaparast ◽  
M. Kolahdoozan
Keyword(s):  
Ferric Chloride ◽  
Chloride Solution ◽  
Kinetic Modelling ◽  
Ferric Chloride Solution ◽  
Low Grade
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