Porphyrin production by Arthrobacter globiformis

The production by Arthrobacter globiformis of an extracellular red pigment in synthetic mineral salts medium was governed by both the conditions of aeration and the trace metals present. Pigment production was the same with either ammonium or nitrate as the nitrogen source. Stand flasks gave approximately the same cell yield as shake cultures but growth was much slower and no pigment was produced. Mn++ was required for maximal pigment production. Zn++ and Co++ enhanced pigment production in the presence of Mn++ but could only partially replace it. The pigment was ether-extractable from culture supernatant fluid acidified with acetic acid.Absorption spectra showed peaks at 620, 565, 527, 495, and 395 mμ. These as well as the extraction properties of the pigment coincided with those reported for coproporphyrin-III.

Download Full-text

ASPERGILLUS TERREUS KMBF1501 A POTENTIAL PIGMENT PRODUCER UNDER SUBMERGED FERMENTATION

International Journal of Pharmacy and Pharmaceutical Sciences ◽

10.22159/ijpps.2017v9i4.16176 ◽

2017 ◽

Vol 9 (4) ◽

pp. 38 ◽

Cited By ~ 5

Author(s):

P. Akilandeswari ◽

B. V. Pradeep

Keyword(s):

Nitrogen Source ◽

Aspergillus Terreus ◽

Inorganic Nitrogen ◽

Pigment Production ◽

High Yield ◽

Dihydrogen Phosphate ◽

Malt Extract ◽

Mineral Salts ◽

Potato Dextrose Broth ◽

Inoculum Age

Objective: The present study was aimed to identify the fungal isolate from soil and to understand the different optimized parameters better to facilitate the pigment production that has high yield and stability.Methods: Aspergillus sp. was isolated from Western Ghats soil by the conventional serial dilution technique and assessed as a potential pigment producer. Different broth medium such as potato dextrose broth (PDB), czapek-dox broth (CDB), malt extract broth (MEB), rose bengal broth (RBB), sabouraud dextrose broth (SDB), yeast malt extract broth (YEMB), pH (3-9), temperature (24, 27, 30, 33, 37 and 40 °C), carbon (lactose,glucose,sucrose, maltose, galactose and fructose) and nitrogen source (peptone, yeast extract, urea and inorganic nitrogen sources like potassium nitrate, ammonium chloride and sodium nitrate), mineral salts such as sodium dihydrogen phosphate (Na2H2Po4), magnesium sulphate (Mg2So4), calcium chloride (CaCl2), copper sulphate (Cu2So4), potassium dihydrogen phosphate (KH2Po4) and manganese sulphate (Mn2So4) and inoculum age (2-7 d) of the medium related to high pigment production were analysed.Results: Aspergillus terreus KMBF1501 was identified by ribosomal DNA sequencing showing 99% similarity with other Aspergillus terreus and the Accession number (KX113516) was assigned. The optimum culture conditions for pigment production by Aspergillus terreus KMBF1501 was achieved at pH 5 (0.563±0.012 nm), temperature of 27 °C (0.382±0.001 nm) with glucose (0.501±0.002 nm) as carbon source, peptone (2.147±0.004 nm) as nitrogen source, Mg2SO4 (0.401±0.001 nm)as mineral salt and 4 d (0.324±0.001 nm) of inoculum age in PDB (0.761±0.006 nm).Conclusion: Aspergillus terreus KMBF1501 produced maximum pigment when cultured in modified PDB than in common PDB medium. The high concentration of the pigment can be used for various industrial purposes.

Download Full-text

Pigment Production of Monascus sp. Isolated from Angkak in Semarang Region, Central Java, Indonesia

Indonesian Food and Nutrition Progress ◽

10.22146/ifnp.24256 ◽

2017 ◽

Vol 14 (1) ◽

pp. 52

Author(s):

Endang Kusdiyantini ◽

Soni Nugraha ◽

Arina Lunggani

Keyword(s):

Ammonium Nitrate ◽

Nitrogen Source ◽

Ammonium Chloride ◽

Nitrogen Sources ◽

Dry Weight ◽

Pigment Production ◽

Natural Food ◽

Red Yeast Rice ◽

Red Pigment ◽

Potato Dextrose Broth

Angkak (red fungi rice, red yeast rice) is a traditional fermentation by fungi Monascus in a lot of Asia Countries, which is useful as a natural food colorant. The aim of this research was to obtain pure isolate of red fungus from angkak in Semarang. The growth and the red pigment production of the selected isolate in the different initial medium pH and nitrogen sources were observed. The treatment was done by growing the isolate in PDB (Potato Dextrose Broth) medium at initial pH 3, 5, 7 and 9 and as well as optimization of nitrogen sources with 1% ammonium chloride, 1% ammonium nitrate, and 1% peptone. Analysis of the fungi growth was performed by weighing mycelia biomass using dry weight method and the red pigments were analysis using spectrophotometer at a wavelength (λmax) of 500 nm. The results showed that the highest absorbance value of pigment (0.81) was obtained at pH 7, and the highest value of the cell dry weight was also obtained at pH 7 (1.23 g/L). The results showed that 1% ammonium chloride was the best nitrogen source for absorbance of red pigment production (0.82), but the highest of dry weight (2.56 g/L) was obtained by using medium with 1% ammonium nitrate as a nitrogen source.

Download Full-text

Production of yellow-green fluorescent pigment by Pseudomonas fluorescens

Brazilian Archives of Biology and Technology ◽

10.1590/s1516-89132006000400009 ◽

2006 ◽

Vol 49 (3) ◽

pp. 411-419 ◽

Cited By ~ 16

Author(s):

Gildo Almeida da Silva ◽

Erik Amazonas de Almeida

Keyword(s):

Pseudomonas Fluorescens ◽

Pigment Production ◽

Siderophore Production ◽

Mineral Salts ◽

Fluorescent Pigment ◽

Green Fluorescent

A medium was prepared from brewery waste yeast with and without mineral salts to study growth and yellow-green fluorescent pigment production (YGFP) by Pseudomonas fluorescens. The King's medium used for detection of siderophore production were expressively weaker inductors of YGFP formation when compared to FYE medium. Although FYE and CYE could be used for growth of P. fluorescens, only FYE was an attractive medium for detection of YGFP strain producers.

Download Full-text

Device for Automatic Rapid Harvest of Roller Culture Supernatant Fluid

Applied Microbiology ◽

10.1128/aem.25.6.1008-1010.1973 ◽

1973 ◽

Vol 25 (6) ◽

pp. 1008-1010 ◽

Cited By ~ 7

Author(s):

Ralph E. Smith ◽

Frank Kozoman

Keyword(s):

Culture Supernatant ◽

Supernatant Fluid

Download Full-text

In Vitro Attachment of Salmonella typhimurium to Chicken Cecal Mucus: Effect of Cations and Pretreatment with Lactobacillus spp. Isolated from the Intestinal Tracts of Chickens

Journal of Food Protection ◽

10.4315/0362-028x-61.3.265 ◽

1998 ◽

Vol 61 (3) ◽

pp. 265-271 ◽

Cited By ~ 22

Author(s):

S. E. CRAVEN ◽

D. D. WILLIAMS

Keyword(s):

Salmonella Typhimurium ◽

Chelating Agents ◽

Culture Supernatant ◽

Divalent Cations ◽

Supernatant Fluid ◽

Specific Pathogen Free ◽

Intestinal Mucus ◽

Specific Pathogen ◽

Lactobacillus Spp

The attachment of radiolabeled Salmonella typhimurium 3333/O cells to immobilized cecal mucus from specific-pathogen-free leghorn chickens was determined in the presence of d-mannose. The attachment of S. typhimurium was inhibited by the chelating agents EDTA and citrate and by lanthanum but was enhanced in the presence of the calcium, barium, and manganese divalent cations. Summary findings of the effect of lectins are included. Attachment of lactobacilli, previously isolated from the intestines of chickens, to mucus was also enhanced by calcium and inhibited by chelators. The pretreatment of immobilized mucus with portions of cultures of five of eight strains of lactobacilli inhibited subsequent attachment of the S. typhimurium strain. Spent culture supernatant fluid and/or washed cells from these cultures inhibited attachment, and inhibition was enhanced by preheating the cells or supernatant fluid at 80°C. Results indicate that S. typhimurium mucus attachment not involving mannosyl-dependent receptors is influenced by presence of cations. Lactobacillus spp. isolated from the intestinal tracts of chickens produce cellular and cell-free components that inhibit this form of attachment to chicken intestinal mucus.

Download Full-text

Effect of factors on the red pigment production in the stirred drum bioreactor: Fractional factorial design approach

10.1063/1.5125512 ◽

2019 ◽

Author(s):

Farhan M. Said ◽

Mohamad Al Aamin Razali

Keyword(s):

Factorial Design ◽

Fractional Factorial Design ◽

Pigment Production ◽

Fractional Factorial ◽

Design Approach ◽

Red Pigment

Download Full-text

Influence of the Nutrients on the Biomass and Pigment Production of Chlorociboria aeruginascens

Journal of Fungi ◽

10.3390/jof5020040 ◽

2019 ◽

Vol 5 (2) ◽

pp. 40 ◽

Cited By ~ 3

Author(s):

Stephanie Stange ◽

Susanne Steudler ◽

Hubertus Delenk ◽

Anett Werner ◽

Thomas Walther ◽

...

Keyword(s):

Nitrogen Source ◽

Organic Semiconductors ◽

Fungal Growth ◽

Nitrogen Sources ◽

Soft Rot ◽

Pigment Production ◽

Primary Metabolism ◽

Green Pigment ◽

Organic Nitrogen Source ◽

Complex Organic

The blue-green pigment xylindein, produced by the soft rot fungus Chlorociboria aeruginascens, is of considerable interest for various applications such as the veneer industry or organic semiconductors. The studies presented were performed in order to understand the fungal growth as well as the pigment production of C. aeruginascens. Therefore, various nutrient compositions were investigated. As a result, observations of the formation of xylindein through C. aeruginascens decoupling from growth were made. In the primary metabolism the uncolored biomass is formed. Various carbohydrates were determined as nutrients for the fungus and as a nitrogen source it was observed that the fungus prefers the complex organic nitrogen source, that being yeast extract. Furthermore, it was discovered that the ratio between carbohydrate and nitrogen sources encourages the switch of the metabolism and therewith the production of the blue-green pigment xylindein.

Download Full-text

Pigmentation and autofluorescence of Candida species after growth on tryptophan media

Canadian Journal of Microbiology ◽

10.1139/m74-091 ◽

1974 ◽

Vol 20 (4) ◽

pp. 595-603 ◽

Cited By ~ 3

Author(s):

Stuart Chaskes ◽

Arthur W. Phillips

Keyword(s):

Candida Albicans ◽

Nitrogen Source ◽

Candida Species ◽

Pigment Production ◽

Brown Pigment ◽

Pink Pigment

Candida albicans and six other medically important Candida species were cultured on glucose–salts–biotin media containing tryptophan as the major nitrogen source. Candida albicans, C. tropicalis, and C. parapsilosis produced a pink pigment; C. krusei, C. pseudotropicalis, and C. gulliermondii formed brown pigments. Pigmentation of C. stellatoidea was variable, some strains produced brown pigments and others a pink pigment. Production of pink pigment was catalyzed by light. Cultures incubated in the dark produced only trace amounts of the pink pigment. Iron enhanced pigment production. Intense autofluorescence was observed for strains producing the brown pigment and weak autofluorescence for strains producing the pink pigment.

Download Full-text