scholarly journals ASPERGILLUS TERREUS KMBF1501 A POTENTIAL PIGMENT PRODUCER UNDER SUBMERGED FERMENTATION

2017 ◽  
Vol 9 (4) ◽  
pp. 38 ◽  
Author(s):  
P. Akilandeswari ◽  
B. V. Pradeep
Keyword(s):  
Nitrogen Source ◽  
Aspergillus Terreus ◽  
Inorganic Nitrogen ◽  
Pigment Production ◽  
High Yield ◽  
Dihydrogen Phosphate ◽  
Malt Extract ◽  
Mineral Salts ◽  
Potato Dextrose Broth ◽  
Inoculum Age

Objective: The present study was aimed to identify the fungal isolate from soil and to understand the different optimized parameters better to facilitate the pigment production that has high yield and stability.Methods: Aspergillus sp. was isolated from Western Ghats soil by the conventional serial dilution technique and assessed as a potential pigment producer. Different broth medium such as potato dextrose broth (PDB), czapek-dox broth (CDB), malt extract broth (MEB), rose bengal broth (RBB), sabouraud dextrose broth (SDB), yeast malt extract broth (YEMB), pH (3-9), temperature (24, 27, 30, 33, 37 and 40 °C), carbon (lactose,glucose,sucrose, maltose, galactose and fructose) and nitrogen source (peptone, yeast extract, urea and inorganic nitrogen sources like potassium nitrate, ammonium chloride and sodium nitrate), mineral salts such as sodium dihydrogen phosphate (Na2H2Po4), magnesium sulphate (Mg2So4), calcium chloride (CaCl2), copper sulphate (Cu2So4), potassium dihydrogen phosphate (KH2Po4) and manganese sulphate (Mn2So4) and inoculum age (2-7 d) of the medium related to high pigment production were analysed.Results: Aspergillus terreus KMBF1501 was identified by ribosomal DNA sequencing showing 99% similarity with other Aspergillus terreus and the Accession number (KX113516) was assigned. The optimum culture conditions for pigment production by Aspergillus terreus KMBF1501 was achieved at pH 5 (0.563±0.012 nm), temperature of 27 °C (0.382±0.001 nm) with glucose (0.501±0.002 nm) as carbon source, peptone (2.147±0.004 nm) as nitrogen source, Mg2SO4 (0.401±0.001 nm)as mineral salt and 4 d (0.324±0.001 nm) of inoculum age in PDB (0.761±0.006 nm).Conclusion: Aspergillus terreus KMBF1501 produced maximum pigment when cultured in modified PDB than in common PDB medium. The high concentration of the pigment can be used for various industrial purposes.

Cell growth and pigment production in suspension cultures of a mycobiont isolated from the lichen Cladonia cristaiella

1995 ◽  
Vol 73 (S1) ◽  
pp. 590-594 ◽  
Author(s):  
Y. Yamamoto ◽  
Y. Kinoshita ◽  
T. Kurokawa ◽  
I. Yoshimura ◽  
V. Ahmadjian ◽  
...  
Keyword(s):  
Cell Growth ◽  
Carbon Source ◽  
Nitrogen Source ◽  
Liquid Culture ◽  
Pigment Production ◽  
Malt Extract ◽  
Nutritional Factors ◽  
Cultural Conditions ◽  
Acetone Extracts ◽  
Affect Cell Growth

This is the first study on the factors that affect cell growth and the production of secondary metabolites of a lichen mycobiont in liquid culture. An ascospore-derived strain of Cladonia cristatella mycobiont accumulated and excreted red pigments into a liquid medium. Growth of the mycobiont was increased by using liquid Lilly–Barnett medium containing 16% (w/v) sucrose as a carbon source, 0.2% (w/v) L-glutamine as a nitrogen source, and 0.2% (w/v) polypeptone, adjusting pH to 5.0 before autoclaving, and incubating cultures at 20 °C. Pigment production by the mycobiont was increased by using liquid Lilly–Barnett medium containing 4% (w/v) sucrose as a carbon source, 0.2% (w/v) L-asparagine as a nitrogen source, and 0.2% (w/v) malt extract, adjusting pH to 5.0 before autoclaving and incubating cultures at 20 °C. All acetone extracts under any cultural conditions yielded similar HPLC chromatograms. We proved no relationship between cell growth and secondary metabolism based on the nutritional factors in the cultured C. cristatella mycobiont. Key words: lichen, suspension culture, Cladonia cristatella mycobiont, red pigment, production, and growth factor.

scholarly journals Enhancement of Bacillus subtilis Lipopeptide Biosurfactants Production through Optimization of Medium Composition and Adequate Control of Aeration

2011 ◽  
Vol 2011 ◽  
pp. 1-6 ◽  
Author(s):  
Dhouha Ghribi ◽  
Semia Ellouze-Chaabouni
Keyword(s):  
Bacillus Subtilis ◽  
Nitrogen Source ◽  
Inorganic Nitrogen ◽  
Medium Composition ◽  
High Yield ◽  
Inorganic Nitrogen Source ◽  
Cultural Conditions ◽  
Commercial Applications ◽  
Surfactin Production ◽  
Lipopeptide Biosurfactants

Interest in biosurfactants has increased considerably in recent years, as they are potentially used in many commercial applications in petroleum, pharmaceuticals, biomedical, and food processing industries. Since improvement of their production was of great importance to reduce the final coast, cultural conditions were analyzed to optimize biosurfactants production from Bacillus subtilis SPB1 strain. A high yield of biosurfactants was obtained from a culture of B. subtilis using carbohydrate substrate as a carbon source; among carbohydrates, glucose enhanced the best surfactin production. The optimum glucose concentration was 40 g/L. Higher amount of biosurfactants was obtained using 5 g/L of urea as organic nitrogen source and applying C/N ratio of 7 with ammonium chloride as inorganic nitrogen source. The highest amount of biosurfactants was recorded with the addition of 2% kerosene. Moreover, it was shown, using an automated full-controlled 2.6 L fermenter, that aeration of the medium, which affected strongly the growth regulated biosurfactants synthesis by the producing cell. So that, low or high aerations lead to a decrease of biosurfactants synthesis yields. It was found that when using dissolved oxygen saturation of the medium at 30%, biosurfactants production reached 4.92 g/L.

Optimization of Growth Medium and Physicochemical Parameters for High-Yield Biomass Production and Active Metabolites from Aspergillus terreus

2021 ◽  
pp. 2924-2930
Author(s):  
Rajitha. P.B. ◽  
Prashantha Naik
Keyword(s):  
Growth Rate ◽  
Biomass Production ◽  
Nutrient Medium ◽  
Physicochemical Parameters ◽  
Aspergillus Terreus ◽  
Maximum Yield ◽  
Nitrogen Sources ◽  
Growth Enhancement ◽  
Malt Extract ◽  
Potato Dextrose Broth

The selection of a nutrient medium and optimization of physicochemical parameters play a crucial role in the growth enhancement and production of bioactive secondary metabolites of fungi. The current study was to evaluate the influence of cultural conditions and physicochemical parameters affecting the growth and biomass production of Aspergillus terreus. Nutrient medium ((Potato dextrose broth, Czapek’s Dox broth, Sabouraud dextrose broth, Potato Carrot broth and malt extract broth) in conjunction with physicochemical parameters such as temperature, pH, nitrogen sources, carbon sources, and salinity were assessed for optimal biomass production. The growth rate of the fungus was measured by determining the mycelial dry weight, and enhancement of antioxidant activity through DPPH scavenging potency in the culture broth. The highest growth rate of the fungus was observed in potato dextrose broth (PDB) compared to the other nutrient media at a significant level (p< 0.05). The present study demonstrated that PDB supplemented with cellulose and urea as carbon and nitrogen sources with a pH of 5.5 and 4% salinity at 28°C are the suitable conditions for maximal growth and biomass production. However, the antioxidants activity was at its peak level in PDA enriched with sucrose and ammonium sulphate at pH 5.5, and 7% salinity at 28°C. Thus, culture conditions, including nutrient components and physicochemical parameters play an important role in the growth rate A. terreus, to exploit the maximum yield for various biomedical applications.

Porphyrin production by Arthrobacter globiformis

1968 ◽  
Vol 14 (10) ◽  
pp. 1095-1103 ◽  
Author(s):  
Katherine S. Middleton ◽  
Haim B. Gunner
Keyword(s):  
Trace Metals ◽  
Nitrogen Source ◽  
Culture Supernatant ◽  
Pigment Production ◽  
Cell Yield ◽  
Arthrobacter Globiformis ◽  
Supernatant Fluid ◽  
Red Pigment ◽  
Mineral Salts ◽  
Extraction Properties

The production by Arthrobacter globiformis of an extracellular red pigment in synthetic mineral salts medium was governed by both the conditions of aeration and the trace metals present. Pigment production was the same with either ammonium or nitrate as the nitrogen source. Stand flasks gave approximately the same cell yield as shake cultures but growth was much slower and no pigment was produced. Mn++ was required for maximal pigment production. Zn++ and Co++ enhanced pigment production in the presence of Mn++ but could only partially replace it. The pigment was ether-extractable from culture supernatant fluid acidified with acetic acid.Absorption spectra showed peaks at 620, 565, 527, 495, and 395 mμ. These as well as the extraction properties of the pigment coincided with those reported for coproporphyrin-III.

scholarly journals Pigment Production of Monascus sp. Isolated from Angkak in Semarang Region, Central Java, Indonesia

2017 ◽  
Vol 14 (1) ◽  
pp. 52
Author(s):  
Endang Kusdiyantini ◽  
Soni Nugraha ◽  
Arina Lunggani
Keyword(s):  
Ammonium Nitrate ◽  
Nitrogen Source ◽  
Ammonium Chloride ◽  
Nitrogen Sources ◽  
Dry Weight ◽  
Pigment Production ◽  
Natural Food ◽  
Red Yeast Rice ◽  
Red Pigment ◽  
Potato Dextrose Broth

Angkak (red fungi rice, red yeast rice) is a traditional fermentation by fungi Monascus in a lot of Asia Countries, which is useful as a natural food colorant.  The aim of this research was to obtain pure isolate of red fungus from angkak in Semarang. The growth and the red pigment production of the selected isolate in the different initial medium pH and nitrogen sources were observed. The treatment was done by growing the isolate in PDB (Potato Dextrose Broth) medium at initial pH 3, 5, 7 and 9 and as well as optimization of nitrogen sources with 1% ammonium chloride, 1% ammonium nitrate, and 1% peptone. Analysis of the fungi growth was performed by weighing mycelia biomass using dry weight method and the red pigments were analysis using spectrophotometer at a wavelength (λmax) of 500 nm. The results showed that the highest absorbance value of pigment (0.81) was obtained at pH 7, and the highest value of the cell dry weight was also obtained at pH 7 (1.23 g/L). The results showed that 1% ammonium chloride was the best nitrogen source for absorbance of red pigment production (0.82), but the highest of dry weight (2.56 g/L) was obtained by using medium with 1% ammonium nitrate as a nitrogen source.

scholarly journals Production of yellow-green fluorescent pigment by Pseudomonas fluorescens

2006 ◽  
Vol 49 (3) ◽  
pp. 411-419 ◽  
Author(s):  
Gildo Almeida da Silva ◽  
Erik Amazonas de Almeida
Keyword(s):  
Pseudomonas Fluorescens ◽  
Pigment Production ◽  
Siderophore Production ◽  
Mineral Salts ◽  
Fluorescent Pigment ◽  
Green Fluorescent

A medium was prepared from brewery waste yeast with and without mineral salts to study growth and yellow-green fluorescent pigment production (YGFP) by Pseudomonas fluorescens. The King's medium used for detection of siderophore production were expressively weaker inductors of YGFP formation when compared to FYE medium. Although FYE and CYE could be used for growth of P. fluorescens, only FYE was an attractive medium for detection of YGFP strain producers.

scholarly journals Influence of the Nutrients on the Biomass and Pigment Production of Chlorociboria aeruginascens

2019 ◽  
Vol 5 (2) ◽  
pp. 40 ◽  
Author(s):  
Stephanie Stange ◽  
Susanne Steudler ◽  
Hubertus Delenk ◽  
Anett Werner ◽  
Thomas Walther ◽  
...  
Keyword(s):  
Nitrogen Source ◽  
Organic Semiconductors ◽  
Fungal Growth ◽  
Nitrogen Sources ◽  
Soft Rot ◽  
Pigment Production ◽  
Primary Metabolism ◽  
Green Pigment ◽  
Organic Nitrogen Source ◽  
Complex Organic

The blue-green pigment xylindein, produced by the soft rot fungus Chlorociboria aeruginascens, is of considerable interest for various applications such as the veneer industry or organic semiconductors. The studies presented were performed in order to understand the fungal growth as well as the pigment production of C. aeruginascens. Therefore, various nutrient compositions were investigated. As a result, observations of the formation of xylindein through C. aeruginascens decoupling from growth were made. In the primary metabolism the uncolored biomass is formed. Various carbohydrates were determined as nutrients for the fungus and as a nitrogen source it was observed that the fungus prefers the complex organic nitrogen source, that being yeast extract. Furthermore, it was discovered that the ratio between carbohydrate and nitrogen sources encourages the switch of the metabolism and therewith the production of the blue-green pigment xylindein.

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