Identification of a viral inhibitor in ground beef

1973 ◽  
Vol 19 (2) ◽  
pp. 177-181 ◽  
Author(s):  
Jack Konowalchuk ◽  
Joan I. Speirs
Keyword(s):  
Bovine Serum ◽  
Infectious Virus ◽  
Fetal Bovine Serum ◽  
Chemical Properties ◽  
Ground Beef ◽  
Beef Extract ◽  
Viral Inhibitor ◽  
Fetal Bovine ◽  
Physical And Chemical ◽  
And Chemical Properties

An inhibitor of coxsackievirus B5 was found in extracts of commercially ground beef; it resembled antibody in physical and chemical properties. Another inhibitor, found in a batch of fetal bovine serum, differed from the inhibitor in beef extract and specific coxsackievirus B5 antibody; it was destroyed at pH 3.0. Chemical bonds between virus and inhibitors in beef extract, fetal bovine serum, or specific antiserum against coxsackievirus B5 were formed during storage at 4 °C. The bonds were broken by treatment with hydrochloric acid at pH 3.0 for 1 h, releasing infectious virus. This finding may be of importance in the recovery of virus from ground beef.

Restricted access molecularly imprinted polymers obtained by bovine serum albumin and/or hydrophilic monomers’ external layers: a comparison related to physical and chemical properties

The Analyst ◽  
2015 ◽  
Vol 140 (22) ◽  
pp. 7768-7775 ◽  
Author(s):  
Mariane Gonçalves Santos ◽  
Gabriel de Oliveira Isac Moraes ◽  
Maurício Gustavo Nakamura ◽  
Álvaro José dos Santos-Neto ◽  
Eduardo Costa Figueiredo
Keyword(s):  
Bovine Serum Albumin ◽  
Sample Preparation ◽  
Molecularly Imprinted Polymers ◽  
Bovine Serum ◽  
Chemical Properties ◽  
Physical And Chemical Properties ◽  
Molecularly Imprinted ◽  
Imprinted Polymers ◽  
Physical And Chemical ◽  
And Chemical Properties

RAMIP-BSA is the best material for sample preparation in terms of selectivity, protein exclusion, and adsorption.

An evaluation of three agents for eluting adsorbed enterovirus from millipore membrane filters

1971 ◽  
Vol 17 (11) ◽  
pp. 1351-1355 ◽  
Author(s):  
Jack Konowalchuk ◽  
Joan I. Speirs
Keyword(s):  
Yeast Extract ◽  
Bovine Serum ◽  
Fetal Bovine Serum ◽  
Cellulose Nitrate ◽  
Membrane Filters ◽  
Beef Extract ◽  
Fetal Bovine ◽  
Millipore Membrane ◽  
High Degree

Several concentrations of yeast extract, beef extract, and fetal bovine serum were compared for ability to elute adsorbed coxsackievirus B5 and poliovirus 1 from HA cellulose nitrate Millipore membrane filters. Elution improved with increases in concentration of eluting agent and volume of eluent. The eluent of choice was undiluted fetal bovine serum because it was compatible with cells and showed a high degree of elution. A 5-ml serum eluate resulted in the recovery of 80–90% of the virus adsorbed by a filter from an infected buffered saline sample.

scholarly journals Genome Sequence of Bovine Polyomavirus 1 Detected in a Salers Cow (Bos taurus) from Catalonia, Spain

2016 ◽  
Vol 4 (1) ◽  
Author(s):  
Nicole Ben Salem ◽  
Bernat Pérez de Val ◽  
Maite Martin ◽  
Ugo Moens ◽  
Bernhard Ehlers
Keyword(s):  
Lymph Node ◽  
Genome Sequence ◽  
Bovine Serum ◽  
Bos Taurus ◽  
Fetal Bovine Serum ◽  
Ground Beef ◽  
Genome Sequences ◽  
Fetal Bovine ◽  
Bovine Polyomavirus

We identified a variant of the first bovine polyomavirus (BPyV1; familyPolyomaviridae) in a lymph node of a Salers cow. As the 2 previously published genome sequences of this virus originated from fetal bovine serum and ground beef, respectively, this is the first BPyV1 genome that could be traced back to an individual.

Statistical criteria of stellar population types

1966 ◽  
Vol 24 ◽  
pp. 101-110
Author(s):  
W. Iwanowska
Keyword(s):  
Stellar Population ◽  
Chemical Properties ◽  
Spectrophotometric Study ◽  
Physical And Chemical Properties ◽  
Population Type ◽  
The Galaxy ◽  
Distribution Parameters ◽  
Physical And Chemical ◽  
Statistical Criteria ◽  
And Chemical Properties

In connection with the spectrophotometric study of population-type characteristics of various kinds of stars, a statistical analysis of kinematical and distribution parameters of the same stars is performed at the Toruń Observatory. This has a twofold purpose: first, to provide a practical guide in selecting stars for observing programmes, second, to contribute to the understanding of relations existing between the physical and chemical properties of stars and their kinematics and distribution in the Galaxy.

Tailoring microstructures of materials through biomimetics

1993 ◽  
Vol 51 ◽  
pp. 500-501
Author(s):  
Mehmet Sarikaya ◽  
Ilhan A. Aksay
Keyword(s):  
Chemical Properties ◽  
Design And Synthesis ◽  
Structure Sensitive ◽  
Macro Scale ◽  
Methods Of Synthesis ◽  
Successive Level ◽  
Engineering Materials ◽  
Physical And Chemical ◽  
And Chemical Properties ◽  
Synthesis Of Materials

Biomimetics involves investigation of structure, function, and methods of synthesis of biological composite materials. The goal is to apply this information to the design and synthesis of materials for engineering applications.Properties of engineering materials are structure sensitive through the whole spectrum of dimensions from nanometer to macro scale. The goal in designing and processing of technological materials, therefore, is to control microstructural evolution at each of these dimensions so as to achieve predictable physical and chemical properties. Control at each successive level of dimension, however, is a major challenge as is the retention of integrity between successive levels. Engineering materials are rarely fabricated to achieve more than a few of the desired properties and the synthesis techniques usually involve high temperature or low pressure conditions that are energy inefficient and environmentally damaging.In contrast to human-made materials, organisms synthesize composites whose intricate structures are more controlled at each scale and hierarchical order.

Response of corn (Zea mays L.) to various organic-based fertilizers in marginal upland of Sta. Rita, Samar

2017 ◽  
pp. 31-43
Author(s):  
Berta Ratilla ◽  
Loreme Cagande ◽  
Othello Capuno
Keyword(s):  
Block Design ◽  
Management Strategies ◽  
Chemical Properties ◽  
Organic Fertilizers ◽  
Growth And Yield ◽  
Physical And Chemical Properties ◽  
Total N ◽  
Randomized Complete Block Design ◽  
Physical And Chemical ◽  
And Chemical Properties

Organic farming is one of the management strategies that improve productivity of marginal uplands. The study aimed to: (1) evaluate effects of various organic-based fertilizers on the growth and yield of corn; (2) determine the appropriate combination for optimum yield; and (3) assess changes on the soil physical and chemical properties. Experiment was laid out in Randomized Complete Block Design, with 3 replications and 7 treatments, namely; T0=(0-0-0); T1=1t ha-1 Evans + 45-30-30kg N, P2O5, K2O ha-1; T2=t ha-1 Wellgrow + 45-30-30kg N, P2O5, K2O ha-1; T3=15t ha-1 chicken dung; T4=10t ha-1 chicken dung + 45-30-30kg N, P2O5, K2O ha-1; T5=15t ha-1 Vermicast; and T6=10t ha-1 Vermicast + 45-30-30kg N, P2O5, K2O ha-1. Application of organic-based fertilizers with or without inorganic fertilizers promoted growth of corn than the control. But due to high infestation of corn silk beetle(Monolepta bifasciata Horns), its grain yield was greatly affected. In the second cropping, except for Evans, any of these fertilizers applied alone or combined with 45-30-30kg N, P2O5, K2O ha-1 appeared appropriate in increasing corn earyield. Soil physical and chemical properties changed with addition of organic fertilizers. While bulk density decreased irrespective of treatments, pH, total N, available P and exchangeable K generally increased more with chicken dung application.

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