Intermediary metabolism of Acinetobacter grown on dialkyl ethers

The microbial dissimilation of long-chain dialkyl ethers by Acinetobacter sp. H01-N involved a terminal methyl group oxidation of the dialkyl ether substrates, resulting in the formation of ether oxygen containing fatty acids of corresponding chain length. An internal carbon–carbon scission of the dialkyl ethers resulted in the formation of end-product ether fatty acids and corresponding dicarboxylic acids. Cellular carbon and energy were derived from the subsequent metabolism of the dicarboxylic acids. Dicarboxylic acid oxidation, activation, and identification of cellular dicarboxylic acids indicated dibasic acids as intermediates in the metabolism of dialkyl ethers.Key words: dialkyl ethers, dicarboxylic acids, fatty acids, Acinetobacter.

Download Full-text

Regulation of leptin secretion from white adipocytes by free fatty acids

AJP Endocrinology and Metabolism ◽

10.1152/ajpendo.00052.2003 ◽

2003 ◽

Vol 285 (3) ◽

pp. E521-E526 ◽

Cited By ~ 42

Author(s):

Philippe G. Cammisotto ◽

Yves Gélinas ◽

Yves Deshaies ◽

Ludwik J. Bukowiecki

Keyword(s):

Fatty Acids ◽

Chain Length ◽

Unsaturated Fatty Acids ◽

Saturated Fatty Acids ◽

Acid Oxidation ◽

Mitochondrial Fatty Acid Oxidation ◽

White Adipocytes ◽

Mitochondrial Fatty Acid ◽

A Chain ◽

Long Chain

Norepinephrine stimulates lipolysis and concurrently inhibits insulin-stimulated leptin secretion from white adipocytes. To assess whether there is a cause-effect relationship between these two metabolic events, the effects of fatty acids were investigated in isolated rat adipocytes incubated in buffer containing low (0.1%) and high (4%) albumin concentrations. Palmitic acid (1 mM) mimicked the inhibitory effects of norepinephrine (1 μM) on insulin (10 nM)-stimulated leptin secretion, but only at low albumin concentrations. Studies investigating the effects of the chain length of saturated fatty acids [from butyric (C4) to stearic (C18) acids] revealed that only fatty acids with a chain length superior or equal to eight carbons effectively inhibited insulin-stimulated leptin secretion. Long-chain mono- and polyunsaturated fatty acids constitutively present in adipocyte triglyceride stores (oleic, linoleic, γ-linolenic, palmitoleic, eicosapentanoic, and docosahexanoic acids) also completely suppressed leptin secretion. Saturated and unsaturated fatty acids inhibited insulin-stimulated leptin secretion with the same potency and without any significant effect on basal secretion. On the other hand, inhibitors of mitochondrial fatty acid oxidation (palmoxirate, 2-bromopalmitate, 2-bromocaproate) attenuated the stimulatory effects of insulin on leptin release without reversing the effects of fatty acids or norepinephrine, suggesting that fatty acids do not need to be oxidized by the mitochondria to inhibit leptin release. These results demonstrate that long-chain fatty acids mimic the effects of norepinephrine on leptin secretion and suggest that they may play a regulatory role as messengers between stimulation of lipolysis by norepinephrine and inhibition of leptin secretion.

Download Full-text

Characterization of the binding sites for dicarboxylic acids on bovine serum albumin

Biochemical Journal ◽

10.1042/bj2760569 ◽

1991 ◽

Vol 276 (3) ◽

pp. 569-575 ◽

Cited By ~ 13

Author(s):

J H Tonsgard ◽

S C Meredith

Keyword(s):

Dicarboxylic Acid ◽

Binding Site ◽

Dicarboxylic Acids ◽

Acid Oxidation ◽

Affinity Binding ◽

Reye's Syndrome ◽

Medium Chain ◽

High Affinity ◽

Dodecanedioic Acid ◽

Long Chain

Dicarboxylic acids are prominent features of several diseases, including Reye's syndrome and inborn errors of mitochondrial and peroxisomal fatty acid oxidation. Moreover, dicarboxylic acids are potentially toxic to cellular processes. Previous studies [Tonsgard, Mendelson & Meredith (1988) J. Clin. Invest. 82, 1567-1573] demonstrated that long-chain dicarboxylic acids have a single high-affinity binding site and between one and three lower-affinity sites on albumin. Medium-chain-length dicarboxylic acids have a single low-affinity site. We further characterized dicarboxylic acid binding to albumin in order to understand the potential effects of drugs and other ligands on dicarboxylic acid binding and toxicity. Progesterone and oleate competitively inhibit octadecanedioic acid binding to the single high-affinity site. Octanoate inhibits binding to the low-affinity sites. Dansylated probes for subdomain 2AB inhibit dodecanedioic acid binding whereas probes for subdomain 3AB do not. In contrast, low concentrations of octadecanedioic acid inhibit the binding of dansylated probes to subdomain 3AB and 2AB. L-Tryptophan, which binds in subdomain 3AB, inhibits hexadecanedioic acid binding but has no effect on dodecanedioic acid. Bilirubin and acetylsalicylic acid, which bind in subdomain 2AB, inhibit the binding of medium-chain and long-chain dicarboxylic acids. Our results suggest that long-chain dicarboxylic acids bind in subdomains 2C, 3AB and 2AB. The single low-affinity binding site for medium-chain dicarboxylic acids is in subdomain 2AB. These studies suggest that dicarboxylic acids are likely to be unbound in disease states and may be potentially toxic.

Download Full-text

Engineering of FRT–lacZ fusion constructs: Induction of the Pseudomonas aeruginosa fadAB1 operon by medium and long chain-length fatty acids

Plasmid ◽

10.1016/j.plasmid.2007.12.002 ◽

2008 ◽

Vol 59 (2) ◽

pp. 111-118 ◽

Cited By ~ 9

Author(s):

Mike S. Son ◽

David T. Nguyen ◽

Yun Kang ◽

Tung T. Hoang

Keyword(s):

Fatty Acids ◽

Pseudomonas Aeruginosa ◽

Chain Length ◽

Lacz Fusion ◽

Long Chain

Download Full-text

III. Peroxisomal β-oxidation, PPARα, and steatohepatitis

AJP Gastrointestinal and Liver Physiology ◽

10.1152/ajpgi.2001.281.6.g1333 ◽

2001 ◽

Vol 281 (6) ◽

pp. G1333-G1339 ◽

Cited By ~ 142

Author(s):

Janardan K. Reddy

Keyword(s):

Fatty Acids ◽

Dicarboxylic Acids ◽

Fatty Acyl ◽

Branched Chain Fatty Acids ◽

Oxidation Chain ◽

Branched Chain ◽

Cytochrome P 450 ◽

Oxidation System ◽

Long Chain ◽

Chain Fatty Acids

Peroxisomes are involved in the β-oxidation chain shortening of long-chain and very-long-chain fatty acyl-CoAs, long-chain dicarboxylyl-CoAs, the CoA esters of eicosanoids, 2-methyl-branched fatty acyl-CoAs, and the CoA esters of the bile acid intermediates, and in the process, they generate H2O2. There are two complete sets of β-oxidation enzymes present in peroxisomes, with each set consisting of three distinct enzymes. The classic PPARα-regulated and inducible set participates in the β-oxidation of straight-chain fatty acids, whereas the second noninducible set acts on branched-chain fatty acids. Long-chain and very-long-chain fatty acids are also metabolized by the cytochrome P-450 CYP4A ω-oxidation system to dicarboxylic acids that serve as substrates for peroxisomal β-oxidation. Evidence derived from mouse models of PPARα and peroxisomal β-oxidation deficiency highlights the critical importance of the defects in PPARα-inducible β-oxidation in energy metabolism and in the development of steatohepatitis.

Download Full-text

Effect of different long-chain fatty acids on cholecystokinin releasein vitroand energy intake in free-living healthy males

British Journal Of Nutrition ◽

10.1017/s0007114511006003 ◽

2012 ◽

Vol 108 (4) ◽

pp. 755-758 ◽

Cited By ~ 12

Author(s):

Charlotte J. Harden ◽

Adam N. Jones ◽

Tannia Maya-Jimenez ◽

Margo E. Barker ◽

Natalie J. Hepburn ◽

...

Keyword(s):

Fatty Acids ◽

Chain Length ◽

Energy Intake ◽

Acyl Chain ◽

Dependent Manner ◽

Long Chain Fatty Acids ◽

Long Chain ◽

Healthy Males ◽

Cck Release ◽

Chain Fatty Acids

Long-chain fatty acids have been shown to suppress appetite and reduce energy intake (EI) by stimulating the release of gastrointestinal hormones such as cholecystokinin (CCK). The effect of NEFA acyl chain length on these parameters is not comprehensively understood. Anin vitroscreen tested the capacity of individual NEFA (C12 to C22) to trigger CCK release. There was a gradient in CCK release with increasing chain length. DHA (C22) stimulated significantly (P < 0·01) more CCK release than all other NEFA tested. Subsequently, we conducted a randomised, controlled, crossover intervention study using healthy males (n18). The effects of no treatment (NT) and oral doses of emulsified DHA-rich (DHA) and oleic acid (OA)-rich oils were compared using 24 h EI as the primary endpoint. Participants reported significantly (P = 0·039) lower total daily EI (29 % reduction) with DHA compared to NT. There were no differences between DHA compared to OA and OA compared to NT. There was no between-treatment difference in the time to, or EI of, the first post-intervention eating occasion. It is concluded that NEFA stimulate CCK release in a chain length-dependent manner up to C22. These effects may be extended to thein vivosetting, as a DHA-based emulsion significantly reduced short-term EI.

Download Full-text

Structural Homogeneity in Unsaturated Fatty Acids of Marine Lipids. A Review

Journal of the Fisheries Research Board of Canada ◽

10.1139/f64-021 ◽

1964 ◽

Vol 21 (2) ◽

pp. 247-254 ◽

Cited By ~ 45

Author(s):

R. G. Ackman

Keyword(s):

Fatty Acids ◽

Double Bond ◽

Polyunsaturated Fatty Acids ◽

Chain Length ◽

Unsaturated Fatty Acids ◽

Analytical Data ◽

Double Bonds ◽

Marine Origin ◽

Methyl Group ◽

Marine Lipids

Consideration of recent analytical data supports the conclusion that the longer-chain polyunsaturated fatty acids of marine origin are all structurally homogeneous in that the double bonds are cis, the double bonds methylene interrupted, and that, with the exception of the C16 chain length, the ultimate double bond will normally be three, six or nine carbon atoms removed from the terminal methyl group.

Download Full-text

EXTRAMITOCHONDRIAL SHORTENING OF LONG CHAIN FATTY ACIDS AND CARNITINE-DEPENDENT FATTY ACID OXIDATION

Carnitine Biosynthesis, Metabolism, and Functions ◽

10.1016/b978-0-12-267060-2.50017-1 ◽

1980 ◽

pp. 127-142 ◽

Cited By ~ 1

Author(s):

H. OSMUNDSEN ◽

RENATA Z. CHRISTIANSEN ◽

J. BREMER

Keyword(s):

Fatty Acids ◽

Fatty Acid ◽

Fatty Acid Oxidation ◽

Acid Oxidation ◽

Long Chain Fatty Acids ◽

Long Chain ◽

Chain Fatty Acids

Download Full-text

Nrf2 affects the efficiency of mitochondrial fatty acid oxidation

Biochemical Journal ◽

10.1042/bj20130863 ◽

2014 ◽

Vol 457 (3) ◽

pp. 415-424 ◽

Cited By ~ 116

Author(s):

Marthe H. R. Ludtmann ◽

Plamena R. Angelova ◽

Ying Zhang ◽

Andrey Y. Abramov ◽

Albena T. Dinkova-Kostova

Keyword(s):

Fatty Acids ◽

Fatty Acid ◽

Fatty Acid Oxidation ◽

Saturated Fatty Acids ◽

Acid Oxidation ◽

Mitochondrial Fatty Acid Oxidation ◽

Atp Production ◽

Mitochondrial Fatty Acid ◽

Mitochondrial Oxidation ◽

Long Chain

Transcription factor Nrf2 affects fatty acid oxidation; the mitochondrial oxidation of long-chain (palmitic) and short-chain (hexanoic) saturated fatty acids is depressed in the absence of Nrf2 and accelerated when Nrf2 is constitutively activated, affecting ATP production and FADH2 utilization.

Download Full-text

Recent Advances in the Pathophysiology of Fatty Acid Oxidation Defects: Secondary Alterations of Bioenergetics and Mitochondrial Calcium Homeostasis Caused by the Accumulating Fatty Acids

Frontiers in Genetics ◽

10.3389/fgene.2020.598976 ◽

2020 ◽

Vol 11 ◽

Author(s):

Alexandre Umpierrez Amaral ◽

Moacir Wajner

Keyword(s):

Fatty Acids ◽

Fatty Acid ◽

Fatty Acid Oxidation ◽

Calcium Homeostasis ◽

Acid Oxidation ◽

Cultured Fibroblasts ◽

Medium Chain ◽

Chain Acyl ◽

Energy Deprivation ◽

Long Chain

Deficiencies of medium-chain acyl-CoA dehydrogenase, mitochondrial trifunctional protein, isolated long-chain 3-hydroxyacyl-CoA dehydrogenase, and very long-chain acyl-CoA dehydrogenase activities are considered the most frequent fatty acid oxidation defects (FAOD). They are biochemically characterized by the accumulation of medium-chain, long-chain hydroxyl, and long-chain fatty acids and derivatives, respectively, in tissues and biological fluids of the affected patients. Clinical manifestations commonly include hypoglycemia, cardiomyopathy, and recurrent rhabdomyolysis. Although the pathogenesis of these diseases is still poorly understood, energy deprivation secondary to blockage of fatty acid degradation seems to play an important role. However, recent evidence indicates that the predominant fatty acids accumulating in these disorders disrupt mitochondrial functions and are involved in their pathophysiology, possibly explaining the lactic acidosis, mitochondrial morphological alterations, and altered mitochondrial biochemical parameters found in tissues and cultured fibroblasts from some affected patients and also in animal models of these diseases. In this review, we will update the present knowledge on disturbances of mitochondrial bioenergetics, calcium homeostasis, uncoupling of oxidative phosphorylation, and mitochondrial permeability transition induction provoked by the major fatty acids accumulating in prevalent FAOD. It is emphasized that further in vivo studies carried out in tissues from affected patients and from animal genetic models of these disorders are necessary to confirm the present evidence mostly achieved from in vitro experiments.

Download Full-text