Genetic transformation of Chainia and heat attenuation of its restriction system

1991 ◽  
Vol 37 (9) ◽  
pp. 713-715 ◽  
Author(s):  
Vijay M. Chauthaiwale ◽  
Pranav R. Vyas ◽  
Vasanti V. Deshpande
Keyword(s):  
Genetic Transformation ◽  
Key Words ◽  
Broad Host Range ◽  
Transformation System ◽  
Restriction System ◽  
Plasmid Replicon ◽  
Commercially Important ◽  
Low Efficiency ◽  
The Many ◽  
Restriction Modification

A PEG-mediated transformation system for Chainia (NCL 82-5-1) was develolped using a broad host range Streptomyces vector, pIJ702. Protoplasts prepared from Chainia (NCL 82-5-1) were regenerated with 5% efficiency. Transformation of the protoplasts with pIJ702 gave 10–20 transformants/μg DNA. The low efficiency of transformation is attributed to a restriction system in Chainia; this could be inhibited by treating the protoplasts at 42 °C for 10 min just before transformation. The yield of transformants increased 100-fold when pIJ702 was modified by passage in Chainia. Because the plasmid replicon was functional in Chainia and the modified plasmid was stably maintained, the transformation system should be useful for self-cloning in Chainia NCL 82-5-1 of the many commercially important enzymes this strain is known to produce. Key words: Chainia, transformation, Streptomyces, pIJ702 restriction modification, heat attenuation.

scholarly journals Genetic Transformation in Peach (Prunus persica L.): Challenges and Ways Forward

Plants ◽  
2020 ◽  
Vol 9 (8) ◽  
pp. 971
Author(s):  
Angela Ricci ◽  
Silvia Sabbadini ◽  
Humberto Prieto ◽  
Isabel MG Padilla ◽  
Chris Dardick ◽  
...  
Keyword(s):  
Genetic Transformation ◽  
Prunus Persica ◽  
Genetic System ◽  
High Rate ◽  
Transformation System ◽  
General Applicability ◽  
Breeding Programs ◽  
Peach Breeding ◽  
Low Efficiency ◽  
High Quality Genome

Almost 30 years have passed since the first publication reporting regeneration of transformed peach plants. Nevertheless, the general applicability of genetic transformation of this species has not yet been established. Many strategies have been tested in order to obtain an efficient peach transformation system. Despite the amount of time and the efforts invested, the lack of success has significantly limited the utility of peach as a model genetic system for trees, despite its relatively short generation time; small, high-quality genome; and well-studied genetic resources. Additionally, the absence of efficient genetic transformation protocols precludes the application of many biotechnological tools in peach breeding programs. In this review, we provide an overview of research on regeneration and genetic transformation in this species and summarize novel strategies and procedures aimed at producing transgenic peaches. Promising future approaches to develop a robust peach transformation system are discussed, focusing on the main bottlenecks to success including the low efficiency of A. tumefaciens-mediated transformation, the low level of correspondence between cells competent for transformation and those that have regenerative competence, and the high rate of chimerism in the few shoots that are produced following transformation.

PERILAKU PEDAGANG MUSLIM DALAM TINJAUAN ETIKA BISNIS ISLAM (KASUS DI PASAR LOAK JAGALAN KEDIRI)

2019 ◽  
Vol 2 (1) ◽  
Author(s):  
Alwi Musa Muzaiyin
Keyword(s):  
Business Ethics ◽  
Key Words ◽  
Luxury Goods ◽  
The World ◽  
Goods Market ◽  
Islamic Business Ethics ◽  
New Goods ◽  
The Many ◽  
Used Goods ◽  
The City

Trade is a form of business that is run by many people around the world, ranging from trading various kinds of daily necessities or primary needs, to selling the need for luxury goods for human satisfaction. For that, to overcome the many needs of life, they try to outsmart them buy products that are useful, economical and efficient. One of the markets they aim at is the second-hand market or the so-called trashy market. As for a trader at a trashy market, they aim to sell in the used goods market with a variety of reasons. These reasons include; first, because it is indeed to fulfill their needs. Second, the capital needed to trade at trashy markets is much smaller than opening a business where the products come from new goods. Third, used goods are easily available and easily sold to buyer. Here the researcher will discuss the behavior of Muslim traders in a review of Islamic business ethics (the case in the Jagalan Kediri Trashy Market). Kediri Jagalan Trashy Market is central to the sale of used goods in the city of Kediri. Where every day there are more than 300 used merchants who trade in the market. The focus of this research is how the behavior of Muslim traders in the Jagalan Kediri Trashy Market in general. Then, from the large number of traders, of course not all traders have behavior in accordance with Islamic business ethics, as well as traders who are in accordance with the rules of Islamic business ethics. This study aims to determine how the behavior of Muslim traders in the Jagalan Kediri Trashy Market in buying and selling transactions and to find out how the behavior of Muslim traders in the Jagalan Kediri Trashy Market in reviewing Islamic business ethics. Key Words: Trade, loak market, Islamic business

scholarly journals Establishment of a Genetic Transformation System in Guanophilic Fungus Amphichorda guana

2021 ◽  
Vol 7 (2) ◽  
pp. 138
Author(s):  
Min Liang ◽  
Wei Li ◽  
Landa Qi ◽  
Guocan Chen ◽  
Lei Cai ◽  
...  
Keyword(s):  
Genetic Transformation ◽  
Genetic Manipulation ◽  
Major Facilitator Superfamily ◽  
Protoplast Regeneration ◽  
Transformation System ◽  
Regeneration Rate ◽  
Bioactive Natural Products ◽  
Genetics Research ◽  
Genetic Transformation System ◽  
Major Facilitator

Fungi from unique environments exhibit special physiological characters and plenty of bioactive natural products. However, the recalcitrant genetics or poor transformation efficiencies prevent scientists from systematically studying molecular biological mechanisms and exploiting their metabolites. In this study, we targeted a guanophilic fungus Amphichorda guana LC5815 and developed a genetic transformation system. We firstly established an efficient protoplast preparing method by conditional optimization of sporulation and protoplast regeneration. The regeneration rate of the protoplast is up to about 34.6% with 0.8 M sucrose as the osmotic pressure stabilizer. To develop the genetic transformation, we used the polyethylene glycol-mediated protoplast transformation, and the testing gene AG04914 encoding a major facilitator superfamily transporter was deleted in strain LC5815, which proves the feasibility of this genetic manipulation system. Furthermore, a uridine/uracil auxotrophic strain was created by using a positive screening protocol with 5-fluoroorotic acid as a selective reagent. Finally, the genetic transformation system was successfully established in the guanophilic fungus strain LC5815, which lays the foundation for the molecular genetics research and will facilitate the exploitation of bioactive secondary metabolites in fungi.

Establishment of efficient callus genetic transformation system for Pyrus armeniacaefolia

2021 ◽  
Vol 289 ◽  
pp. 110429
Author(s):  
Xinhui Wang ◽  
Fengli Zhou ◽  
Jianlong Liu ◽  
Wenqian Liu ◽  
Shaoling Zhang ◽  
...  
Keyword(s):  
Genetic Transformation ◽  
Transformation System ◽  
Genetic Transformation System

scholarly journals Overcoming the Barrier of Low Efficiency during Genetic Transformation of Streptococcus mitis

2016 ◽  
Vol 07 ◽  
Author(s):  
Gabriela Salvadori ◽  
Roger Junges ◽  
Donald A. Morrison ◽  
Fernanda C. Petersen
Keyword(s):  
Genetic Transformation ◽  
Streptococcus Mitis ◽  
Low Efficiency

Predation by Sharks on the Grey Seal (Halichoerus grypus) in Eastern Canada

1983 ◽  
Vol 40 (3) ◽  
pp. 267-271 ◽  
Author(s):  
Paul Brodie ◽  
Brian Beck
Keyword(s):  
Key Words ◽  
Population Size ◽  
Halichoerus Grypus ◽  
Longline Fishery ◽  
Grey Seal ◽  
Eastern Canada ◽  
The Past ◽  
Harbour Seals ◽  
Commercially Important ◽  
Important Fish

The increase in population size of the grey seal (Halichoerus grypus) off eastern Canada over the past 20 yr may be attributed to a decrease in shark stocks, their supposed predators. Reduction of the shark population is presumed to have resulted from a directed longline fishery and, of greater significance, from a change in the fishery for swordfish (Xiphias gladius) from selective harpooning to pelagic longlining, which has produced a large bycatch of sharks. The resulting enhanced survival of grey seals is reflected in greater infestation of commercially important fish species by the codworm (Phocanema decipiens).Key words: grey seals, harbour seals, sharks, swordfish, codworm, predation, fisheries

scholarly journals Establishment of a Tractable Genetic Transformation System in Veillonella spp.

2012 ◽  
Vol 78 (9) ◽  
pp. 3488-3491 ◽  
Author(s):  
Jinman Liu ◽  
Zhoujie Xie ◽  
Justin Merritt ◽  
Fengxia Qi
Keyword(s):  
Escherichia Coli ◽  
Genetic Transformation ◽  
Shuttle Vector ◽  
Transformation System ◽  
Genetic Studies ◽  
Content Type ◽  
Genetic Transformation System

ABSTRACTWe have constructed the firstEscherichia coli-Veillonellashuttle vector based on an endogenous plasmid (pVJL1) isolated from a clinicalVeillonellastrain. A highly transformableVeillonellastrain was also identified. Both the shuttle vector and the transformable strain should be valuable tools for futureVeillonellagenetic studies.

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