Short-term recovery of Bradyrhizobium japonicum during an inoculation process using mineral microgranules

1994 ◽  
Vol 40 (4) ◽  
pp. 322-325 ◽  
Author(s):  
Georges Fouilleux ◽  
Cécile Revellin ◽  
Gérard Catroux
Keyword(s):  
Glycine Max ◽  
Moisture Content ◽  
Bradyrhizobium Japonicum ◽  
Water Retention ◽  
Short Term ◽  
Relative Water ◽  
Glycine Max L ◽  
Retention Characteristics ◽  
Granule Type ◽  
Commercial Inoculants

Soybean (Glycine max (L.) Merr.) can be inoculated by delivering inoculant mixed with mineral microgranules to the seedbed. An average decline of 0.61 log units in the number of viable Bradyrhizobium japonicum was found during the 1sth, using available commercial inoculants and granules. These losses were shown to be influenced by inoculant type (peat based, liquid, lyophilized) and granule type. When mixed with granules, a peat-based inoculant was found to be desiccated immediately and subjected to a water potential as low as −170 MPa. Bradyrhizobium japonicum recovery was improved when the moisture content of the granules increased. It was concluded that water suction by granules can subject the bacteria to rapid desiccation and significantly decrease their number. Thus, the nature of inoculant and granular material and their relative water retention characteristics must be taken into account to improve the efficiency of this inoculation process.Key words: Bradyrhizobium japonicum, granular inoculant, inoculation, soybean, survival.

Effect of numbers of Bradyrhizobium japonicum applied in commercial inoculants on soybean seed yield in Ontario

1992 ◽  
Vol 38 (6) ◽  
pp. 588-593 ◽  
Author(s):  
D. J. Hume ◽  
D. H. Blair
Keyword(s):  
Glycine Max ◽  
Seed Yield ◽  
Bradyrhizobium Japonicum ◽  
Field Experiments ◽  
Soybean Seed ◽  
Yield Response ◽  
Cubic Equation ◽  
Glycine Max L ◽  
Seed Yields ◽  
Commercial Inoculants

In the absence of Bradyrhizobium japonicum populations in the soil, yields of field-grown soybean (Glycine max (L.) Merrill) usually respond to inoculation with B. japonicum. The objective of this research was to determine the relationship between numbers of B. japonicum per seed in inoculants and soybean nodulation and yield. A total of six field experiments were conducted in 1989 and 1990 on new soybean soils. In dilution trials, Grip inoculant was applied to provide approximately 106, 105, 104, and 103B. japonicum per seed at two locations in 1989. Nodule number and mass, as well as seed yield, increased curvilinearly upward with increasing log10 most probable numbers (MPNs) of B. japonicum. The yield response curve was best fit by a cubic equation, which accounted for 97% of the variation in yield. Seed yields increased 19% (1.83 to 2.13 Mg/ha) from 105 to 106B. japonicum per seed. In field experiments involving 8 commercial inoculants in 1989 and 10 in 1990, and conducted at two locations in each year, responses to increasing log MPNs in the inoculants also were concave upwards and cubic. In the two years, 78 and 46% of the yield variation was accounted for by log MPN per seed. Increasing MPN per seed from 105 to 106 improved yields in first-time fields by an average of 24%, indicating the present minimum standard of 105B. japonicum per seed should be increased. Key words: most probable numbers, response to inoculation, nodulation, Glycine max (L.) Merrill.

scholarly journals Diversidad de los rizobios que nodulan la soja en los suelos de la Pampa húmeda e identificación de cepas para la fabricación de inoculantes comerciales

2016 ◽  
Author(s):  
◽  
Graciela N. Pastorino
Keyword(s):  
Glycine Max ◽  
Bradyrhizobium Japonicum ◽  
Glycine Max L

Las labores culturales, provocan modificaciones de las propiedades físicas, químicas y biológicas de los suelos. Estas se clasifican en: labranza convencional, labranza vertical y siembra directa. Cada tecnología de manejo genera estreses a los que las poblaciones microbianas se adaptan, como resultado de cambios morfológicos, fisiológicos y genéticos. La inoculación de la soja (Glycine max [L.] Merr) es una tecnología que se introdujo en la Argentina junto con el cultivo y por ello los inoculantes fueron el vehículo de ingreso de las cepas exóticas de Bradyrhizobium, que una vez incorporadas al suelo, se adaptaron y establecieron dando origen a las poblaciones de rizobios naturalizadas. Lo que condujo a generar diversidad a nivel del genoma de los bradyrizobios. El objetivo de este trabajo fue evaluar la diversidad de los simbiontes de soja que se encuentran en la población del suelo y el rol que cumple como recurso en la selección de cepas para la producción comercial de inoculantes. Se evaluaron dos muestras de suelo que durante los últimos 8 años fueron trabajados con siembra directa (SD) y con labranza convencional (LC). Se realizó el recuento y aislamiento de rizobios. Los aislados se caracterizaron fisiológica y genéticamente. La diversidad se evaluó mediante la amplificación de las secuencias BOX A1R. Se seleccionaron 52 aislados que se identificaron amplificando las regiones RSα y nifD y la secuenciación del fragmento ITS 16S - 23S rDNA. El recuento de rizobios mostró que en el suelo bajo SD y antecesor soja la población fue mayor que en la muestra de suelo bajo LC y antecesor maíz. La caracterización fisiológica de 200 aislados reflejó que los suelos LC contuvieron un mayor número de cepas tolerantes a salinidad y alta temperatura. Además un porcentaje mayor de aislados del suelo LC, respecto del suelo bajo SD, resultó con capacidad de sobrevivencia sobre semilla superior a la cepa control E109. Los aislados además presentaron diferencias en la nodulación y fijación de nitrógeno, en la tolerancia a glifosato y en la producción de ácido indol acético (AIA). Sólo un aislado mostró capacidad de solubilización de fósforo. El análisis del dendrograma generado con los fingerprints BOX A1R, mostró que hay mayor diversidad en los suelos bajo SD. Sobre los 52 aislados analizados en base a la secuencia del ITS se encontró que el 70 % son Bradyrhizobium japonicum y 30 % B. elkanii. Se identificaron 4 aislados que comparten características genéticas de ambas especies de Bradyrhizobium. En esta tesis se confirmó que en los suelos con historia de cultivo de soja, manejados bajo distintos sistemas de labranzas, se indujeron cambios a nivel del genoma de las cepas de rizobios que contribuyó a modificar la diversidad de la población. Las poblaciones naturalizadas son un recurso para la identificación de rizobios con características superiores para la fabricación de inoculantes, sin embargo es necesario profundizar las evaluaciones de la supervivencia, tolerancia, competitividad y promoción del crecimiento vegetal de rizobios naturalizados para emplearlos como bioinoculantes.

INFLUENCE OF SINGLE STRAINS AND A COMMERCIAL MIXTURE OF Bradyrhizobium japonicum ON GROWTH, NITROGEN ACCUMULATION AND NODULATION OF TWO EARLY-MATURING SOYBEAN CULTIVARS

1988 ◽  
Vol 68 (2) ◽  
pp. 411-418 ◽  
Author(s):  
L. D. BAILEY
Keyword(s):  
Glycine Max ◽  
Soil Temperature ◽  
Bradyrhizobium Japonicum ◽  
Nodule Formation ◽  
Growth Stages ◽  
Nitrogen Accumulation ◽  
Plant Nitrogen ◽  
Commercial Mixture ◽  
Early Maturing ◽  
Glycine Max L

Seven single strains and a commercial mixture of Bradyrhizobium japonicum were evaluated in association with two early-maturing Canadian soybean (Glycine max (L.) Merrill) cultivars, Maple Presto and Maple Amber. Inoculated and uninoculated plants were grown in pails outdoors. Soil temperature at 15 cm depth was monitored throughout the experiment. At the V2, V3, R2 and R4 growth stages, whole plants were removed from the pails. Nodules were counted and weighed; roots and tops were separated, weighed and analyzed for total nitrogen. Bradyrhizobium japonicum strains 61A148, 61A196, 61A194 and 61A155 were similar in effectiveness, but superior to strains 61A124a, 61A118b, 61A101c and the commercial mixture in earliness of nodule formation, number and weight of nodules per plant, and in promoting greater root and top growth and plant nitrogen accumulation. There were indications that soil temperature may have affected nodulation. Maple Amber showed the greater potential for symbiotic nitrogen fixation. This cultivar supported earlier nodulation, had a greater number of nodules, accumulated more nitrogen in the tops and roots and had greater growth than Maple Presto.Key words: Soybean, Glycine max (L.) Merrill, soil temperature, soybean growth stages, Bradyrhizobium, nodulation

Effects of soil nitrogen status and rate of inoculation on the establishment of populations of Bradyrhizobium japonicum and on the nodulation of soybeans

1989 ◽  
Vol 40 (4) ◽  
pp. 753
Author(s):  
J Brockwell ◽  
RR Gault ◽  
LJ Morthorpe ◽  
MB Peoples ◽  
GL Turner ◽  
...  
Keyword(s):  
Glycine Max ◽  
Soil Nitrogen ◽  
Bradyrhizobium Japonicum ◽  
Clay Soil ◽  
Mineral Nitrogen ◽  
Soil Mineral ◽  
Soil Mineral Nitrogen ◽  
Nitrogen Status ◽  
Mineral N ◽  
Glycine Max L

Soybeans (Glycine max [L.] Merrill cv. Forrest) were grown under irrigation on a well-structured grey clay soil, previously free of Bradyrhizobium japonicum and containing relatively high levels of mineral N, at Trangie, N.S.W. There were two soil pretreatments, pre-cropped (which had the effect of reducing the level of mineral nitrogen in the soil) and pre-fallowed, and four rates of inoculation (B. japonicum CB 1809 - nil, 0.01 X, 1.OX [=normal] and 100X).Mineral nitrogen (0-10 cm) initially was higher in pre-fallowed soil than in pre-cropped soil (37.6 v. 18.5 mg N per kg). Depletion of mineral nitrogen occurred more rapidly in pre-fallowed treatments, so that, 7 days after harvest, mineral-N in pre-cropped soil was significantly higher than in pre-fallowed soil (14.4 v. 10.6 mg per kg).With high levels of soil mineral nitrogen, colonization of seedling rhizospheres by rhizobia and plant nodulation were diminished. These effects were ameliorated but not eliminated by increased rates of inoculation. The development of the symbiosis was also impeded by lower rates of inoculation (0.01 X, 1.OX).

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