The production of α-hydroxyglutaric acid from glutamic acid by cell-free preparations of Peptococcus aerogenes

1969 ◽  
Vol 47 (12) ◽  
pp. 1103-1107 ◽  
Author(s):  
W. M. Johnson ◽  
D. W. S. Westlake
Keyword(s):  
Mass Spectrometry ◽  
Infrared Spectroscopy ◽  
Thin Layer ◽  
Glutamic Acid ◽  
Anion Exchange Chromatography ◽  
Exchange Chromatography ◽  
Factors Affecting ◽  
The One ◽  
Layer Chromatography ◽  
Hydroxyglutaric Acid

Active cell-free extracts of Peptococcus aerogenes were prepared which metabolized glutamic acid to α-hydroxyglutaric acid. Factors affecting the formation of this intermediate were studied by following the conversion of glutamic acid labelled with 14C in the one or five positions. The results of these experiments revealed that the production of α-hydroxyglutaric acid from glutamic acid by cell-free extracts was NAD-dependent. The labelled α-hydroxyglutaric acid produced by NAD-supplemented extracts was purified by anion exchange chromatography and identified by several methods including paper and thin-layer chromatography, mass spectrometry, and infrared spectroscopy. A pathway has been proposed for the conversion of glutamic acid to α-hydroxyglutaric acid by cell-free extracts of P. aerogenes.

scholarly journals Chemical identity of tryptensin with angiotensin

1980 ◽  
Vol 187 (3) ◽  
pp. 647-653 ◽  
Author(s):  
K Arakawa ◽  
M Yuki ◽  
M Ikeda
Keyword(s):  
Amino Acid ◽  
Thin Layer ◽  
Gel Filtration ◽  
Deae Cellulose ◽  
Anion Exchange Chromatography ◽  
Exchange Chromatography ◽  
Cation Exchange Chromatography ◽  
Human Plasma Protein ◽  
Plasma Protein Fraction ◽  
Layer Chromatography

Tryptensin, a vasopressor substance generated from human plasma protein fraction IV-4 by trypsin, has been isolated and the amino acid composition analysed. The procedures used for the isolation were: (a) adsorption of the formed tryptensin on Dowex 50W (X2; NH4+ form); (b) gel filtration through Sephadex G-25; (c) cation-exchange chromatography on CM-cellulose; (d) anion-exchange chromatography on DEAE-cellulose; (e) re-chromatography on CM-cellulose; (f) gel filtration on Bio-Gel P-2; (g) partition chromatography on high-pressure liquid chromatography. The homogeneity of the isolated tryptensin was confirmed by thin-layer chromatography and thin-layer electrophoresis. The amino acid analysis of the hydrolysate suggested the following proportional composition: Asp, 1; Val, 1; Ile, 1; Tyr, 1; Phe, 1; His, 1; Arg, 1; Pro, 1. This composition is identical with that of human angiotensin.

Metabolism of 2′,5-Dichloro-4′-Nitrosalicylanilide (Bayer 73) in Rainbow Trout (Salmo gairdneri)

1975 ◽  
Vol 32 (4) ◽  
pp. 515-522 ◽  
Author(s):  
Charles N. Statham ◽  
John J. Lech
Keyword(s):  
Mass Spectrometry ◽  
Infrared Spectroscopy ◽  
Rainbow Trout ◽  
Thin Layer ◽  
Acid Hydrolysis ◽  
Water Transfer ◽  
Salmo Gairdneri ◽  
High Degree ◽  
Layer Chromatography ◽  
Water Ratio

Uptake, metabolism, and elimination of 2′,5-dichloro-4′-nitrosalicylanilide (Bayer 73) was studied in rainbow trout (Salmo gairdneri) exposed to 14C Bayer 73 in water. Transfer of trout to fresh water after exposure to Bayer 73 for 12 h resulted in the disappearance of 14C from most tissues after 48–72 h. A 24-h 14C bile to water ratio of 10,000:1 indicated a high degree of biliary concentration. A single polar 14C metabolite was purified from bile. Analysis by analytical thin-layer chromatography, β-glucuronidase hydrolysis, acid hydrolysis, infrared spectroscopy, and mass spectrometry indicated that the material was the glucuronide conjugate of Bayer 73.

Herbicidal Metabolites from a Soil-Dwelling Fungus (Scopulariopis brumptii)

Weed Science ◽  
1989 ◽  
Vol 37 (1) ◽  
pp. 123-128 ◽  
Author(s):  
Jamin Huang ◽  
Alan R. Putnam ◽  
Georgina M. Werner ◽  
Saroj K. Mishra ◽  
Curt Whitenack
Keyword(s):  
Mass Spectrometry ◽  
Fourier Transform ◽  
Infrared Spectroscopy ◽  
Thin Layer ◽  
Foliar Application ◽  
White Mustard ◽  
Complete Control ◽  
Ft Ir ◽  
Layer Chromatography ◽  
Hydroxybenzyl Alcohol

The fungusScopulariopis brumptiiSalvanet-Duval (MSU 42018) was isolated from a soil sample collected from the rhizosphere of a potted asparagus plant. When cultured 7 days in A-9 broth medium, the organism produced compounds phytotoxic to seeding dicotyledonous weeds (via foliar application) and toChlamydomonas reinhardtiiDangeard. Three herbicidal metabolites, 3-hydroxybenzyl alcohol, 2-methylhydroquinone, and (+)-epiepoformin, were isolated by column and thin-layer chromatography (TLC) and structures confirmed by Fourier transform infrared spectroscopy (FT-IR), nuclear magnetic reasonance spectroscopy (NMR), and mass spectrometry (MS). Of the three compounds, (+)-epiepoformin was the most active, providing complete control of redroot pigweed and 88% control of white mustard when applied at 4.4 kg/ha.

scholarly journals Neonatal Urine Screening Program in the Province of Quebec: Technological Upgrade from Thin Layer Chromatography to Tandem Mass Spectrometry

2021 ◽  
Vol 7 (1) ◽  
pp. 18
Author(s):  
Christiane Auray-Blais ◽  
Michel Boutin ◽  
Pamela Lavoie ◽  
Bruno Maranda
Keyword(s):  
Mass Spectrometry ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Clinical Symptoms ◽  
Screening Program ◽  
Compliance Rate ◽  
Inborn Errors ◽  
Organic Acidurias ◽  
Urine Screening ◽  
Layer Chromatography

The Quebec Neonatal Urine Screening Program was initiated in 1971 with overall screening inception of newborns in 1973. Forty-seven years later, over 3.5 million babies have been screened for up to 25 inborn errors of metabolism divided into two groups: (1) urea cycle disorders and organic acidurias; and (2) disorders of amino acid metabolism and transport. The main goal of this preventive genetic medicine program is the detection of treatable diseases before the onset of clinical symptoms. Urine specimens from 21-day-old babies are collected and dried on filter paper by parents at home. The participation is voluntary with a high compliance rate over the years (~90%). Specimens are analyzed by thin layer chromatography (TLC). The main objective of this evaluative research project was to assess the feasibility of a technological upgrade towards mass spectrometry. A 2.85-min flow injection method was devised, normal values established, and abnormal profiles confirmed using second-tier tests. The validated assays are sensitive, specific, and suitable for populational screening, as well as for high-risk screening laboratories. Triple H syndrome, which would not be detected in newborns by blood screening at two days of age was found to be positive in the urine of an affected patient.

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