Structural investigation of the lipopolysaccharide core isolated from a virulent strain of Vibrio ordalii

1985 ◽  
Vol 63 (12) ◽  
pp. 1199-1205 ◽  
Author(s):  
Joseph H. Banoub ◽  
Howard J. Hodder
Keyword(s):  
Nuclear Magnetic Resonance ◽  
Nitrous Acid ◽  
Structural Investigation ◽  
Chromium Trioxide ◽  
Virulent Strain ◽  
Partial Hydrolysis ◽  
Methylation Analysis ◽  
Core Oligosaccharide ◽  
The Core ◽  
Vibrio Ordalii

The structure of the core oligosaccharide of Vibrio ordalii has been investigated. The studies involved the use of nuclear magnetic resonance, methylation analysis, partial hydrolysis with hydrochloric acid, nitrous acid deamination, partial hydrolysis with sulfuric acid, Smith degradation, and oxidation with chromium trioxide. As a result of these studies the following structure is proposed.[Formula: see text]

The structure of the antigenic lipopolysaccharide O-chain produced by Escherichiahermannii ATCC 33650 and 33652

1990 ◽  
Vol 68 (8) ◽  
pp. 1456-1466 ◽  
Author(s):  
Linda M. Beynon ◽  
David R. Bundle ◽  
Malcolm B. Perry
Keyword(s):  
Nuclear Magnetic Resonance ◽  
Magnetic Resonance ◽  
High Resolution ◽  
Molecular Modelling ◽  
Partial Hydrolysis ◽  
Two Dimensional ◽  
Methylation Analysis ◽  
Structure Formula ◽  
Structural Methods ◽  
Nuclear Magnetic

High resolution two-dimensional 1H and 13C nuclear magnetic resonance at 500 MHz was used in combination with molecular modelling to solve the structures of the antigenic O-polysaccharides produced by Escherichiahermannii strains ATCC 33650 and 33652. Classical structural methods such as methylation analysis, selective and partial hydrolysis, and periodate oxidations confirmed that the O-polysaccharides had a branched tetrasaccharide repeating unit with the structure:[Formula: see text]Keywords: Escherichiahermannii, lipopolysaccharide, magnetic resonance, polysaccharide.

Structure of the lipopolysaccharide O-chain of Yersinia enterocolitica serotype O:5,27

1987 ◽  
Vol 65 (1) ◽  
pp. 1-7 ◽  
Author(s):  
Malcolm B. Perry ◽  
Leann L. MacLean
Keyword(s):  
Nuclear Magnetic Resonance ◽  
Yersinia Enterocolitica ◽  
Lipid A ◽  
Optical Rotation ◽  
Periodate Oxidation ◽  
Partial Hydrolysis ◽  
Specific Optical Rotation ◽  
Core Oligosaccharide ◽  
Magnetic Resonance Studies ◽  
Serotype O

The cellular lipopolysaccharide produced by Yersinia enterocolitica serotype O:5,27 was of the S-type and composed of an antigenic O-chain polysaccharide linked through a core oligosaccharide region, which in turn was linked through 3-deoxy-D-manno-octulonosyl units to a lipid A moiety. The O-chain polysaccharide was composed of equal molar amounts of L-rhamnose and D-xylulose. By partial hydrolysis, periodate oxidation, methylation, specific optical rotation, and 13C and 1H nuclear magnetic resonance studies, the structure of the O-chain was established as being a linear backbone of alternating 1,3-linked α-L-rhamnopyranosyl and β-L-rhamnopyranosyl units, to which 2,2-linked β-D-threo-pent-2-ulofuranoside (D-xylulofuranoside) units were present on every L-rhamnopyranosyl residue, as shown below.[Formula: see text]

The structures of the two lipopolysaccharide O-chains produced by Salmonella boecker

1988 ◽  
Vol 66 (10) ◽  
pp. 1066-1077 ◽  
Author(s):  
Jean-Robert Brisson ◽  
Malcolm B. Perry
Keyword(s):  
Molecular Weight ◽  
Nuclear Magnetic Resonance ◽  
Magnetic Resonance ◽  
High Molecular Weight ◽  
Nitrous Acid ◽  
Optical Rotation ◽  
Partial Hydrolysis ◽  
Magnetic Resonance Studies ◽  
High Molecular Weight Polymers ◽  
Nuclear Magnetic

Salmonella boecker, which belongs to group 0:6, 14(H) and shows the antigenic factors 6, 14, [1], and [25], defined by the Kauffmann–White system, produces two lipopolysaccharides differing from each other in the structures of their 0-polysaccharide moieties. By glycose composition, partial hydrolysis, nitrous acid deamination, methylation, optical rotation, and 1H and 13C nuclear magnetic resonance studies, the O-polysaccharides were demonstrated to be high-molecular-weight polymers (I and II) composed of either structurally related repeating tetrasaccharide or repeating pentasaccharide units having the structuresand[Formula: see text][Formula: see text]

Structure of the L1 and L6 core oligosaccharide epitopes of Neisseriameningitidis

1990 ◽  
Vol 68 (7) ◽  
pp. 1029-1034 ◽  
Author(s):  
Jose L. Di Fabio ◽  
Francis Michon ◽  
Jean-Robert Brisson ◽  
Harold J. Jennings
Keyword(s):  
Nuclear Magnetic Resonance ◽  
Nmr Spectroscopy ◽  
Two Dimensional ◽  
Mild Acid Hydrolysis ◽  
Core Oligosaccharide ◽  
One Dimensional ◽  
Nuclear Overhauser Enhancement ◽  
The Core ◽  
Nuclear Overhauser ◽  
Mild Acid

The core oligosaccharides obtained from the lipopolysaccharides of Neisseriameningitidis serotypes 1 and 6 were isolated by mild acid hydrolysis, and their structures elucidated by combined chemical, biochemical, and physical techniques. Use was made of 500 MHz 1H nuclear magnetic resonance measurements in both one-dimensional and two-dimensional modes as well as of nuclear Overhauser enhancement experiments. To assist in the structural assignments, the purified oligosaccharides were also degraded by chemical and enzymatic procedures to smaller fragments. The L1 oligosaccharide is a biantennary partially O-acetylated heptasaccharide in which the larger antenna terminates in a α-D-Galp1 → 4β-D-Galp1 → 4β-D-Glcp unit, whereas the smallest disaccharide antenna carries phosphorylethanolamine groups at O-3 of its heptosyl moiety. The L6 oligosaccharide is also a biantennary partially O-acetylated heptasaccharide but, unlike the L1 core, is terminated by a β-D-GlcpNAcl → 3β-D-Galp1 → 4β-D-Glcp unit in its larger antenna. The heptosyl moiety in the small disaccharide antenna bears a phosphorylethanolamine group at O-7, unlike O-3 for the L1 determinant. Keywords: lipopolysaccharides, Neisseriameningitidis, oligosaccharide epitopes, structure, NMR spectroscopy.

Structural elucidation of the O-specific polysaccharide of the phenol-phase soluble lipopolysaccharide of Vibrio anguillarum

1987 ◽  
Vol 65 (1) ◽  
pp. 19-26 ◽  
Author(s):  
Joseph H. Banoub ◽  
Francis Michon ◽  
Howard J. Hodder
Keyword(s):  
Main Chain ◽  
Chromium Trioxide ◽  
Vibrio Anguillarum ◽  
Side Chain ◽  
Partial Hydrolysis ◽  
Two Dimensional ◽  
Methylation Analysis ◽  
Magnetic Resonance Studies ◽  
Specific Polysaccharide ◽  
Structure Formula

The structure of the O-specific polysaccharide of the phenol-soluble cellular lipopolysaccharide of Vibrio anguillarum has been investigated. The studies involved the use of methylation analysis, partial hydrolysis with 48% hydrogen fluoride, Smith degradation, oxidation with chromium trioxide, and comprehensive proton and carbon-13 nuclear magnetic resonance studies, in which one- and two-dimensional experiments were carried out. As a result of these studies it is proposed that the O-specific polysaccharide of Vibrio anguillarum is composed of a regular heteropolymer, i.e., a main chain of (1→4)-linked 3-acetamido-3,6-dideoxy-β-L-glucose residues alternately substituted through O-2 with side chain residues of 2-acetamido-2,6-dideoxy-α-D-glucose, which seem to be substituted through either O-3 or O-4 with propionyl groups (R), as in the following structure.[Formula: see text]

Glucan isolated from leaves of Althaea officinalis L.

1983 ◽  
Vol 48 (7) ◽  
pp. 2082-2087 ◽  
Author(s):  
Alžbeta Kardošová ◽  
Jozef Rosík ◽  
Rudolf Toman ◽  
Peter Capek
Keyword(s):  
Medicinal Plant ◽  
Periodate Oxidation ◽  
Linear Structure ◽  
Water Soluble ◽  
Partial Hydrolysis ◽  
Methylation Analysis ◽  
Glycosidic Bonds ◽  
Nmr Data ◽  
Althaea Officinalis ◽  
C Nmr

A water-soluble low-molecular D-glucan was isolated from leaves of the medicinal plant marsh-mallow (Althaea officinalis L.). The results of methylation analysis, partial hydrolysis, periodate oxidation, and 13C NMR data indicated a virtually linear structure with α-(1→6) glycosidic bonds.

Textural and pore size analysis of carbonates from integrated core and nuclear magnetic resonance logging: An Arbuckle study

2015 ◽  
Vol 3 (1) ◽  
pp. SA77-SA89 ◽  
Author(s):  
John Doveton ◽  
Lynn Watney
Keyword(s):  
Nuclear Magnetic Resonance ◽  
Relaxation Time ◽  
Magnetic Resonance ◽  
Pore Size ◽  
Relaxation Times ◽  
T2 Relaxation Time ◽  
T2 Relaxation ◽  
Nmr Logging ◽  
The Core ◽  
Nuclear Magnetic

The T2 relaxation times recorded by nuclear magnetic resonance (NMR) logging are measures of the ratio of the internal surface area to volume of the formation pore system. Although standard porosity logs are restricted to estimating the volume, the NMR log partitions the pore space as a spectrum of pore sizes. These logs have great potential to elucidate carbonate sequences, which can have single, double, or triple porosity systems and whose pores have a wide variety of sizes and shapes. Continuous coring and NMR logging was made of the Cambro-Ordovician Arbuckle saline aquifer in a proposed CO2 injection well in southern Kansas. The large data set gave a rare opportunity to compare the core textural descriptions to NMR T2 relaxation time signatures over an extensive interval. Geochemical logs provided useful elemental information to assess the potential role of paramagnetic components that affect surface relaxivity. Principal component analysis of the T2 relaxation time subdivided the spectrum into five distinctive pore-size classes. When the T2 distribution was allocated between grainstones, packstones, and mudstones, the interparticle porosity component of the spectrum takes a bimodal form that marks a distinction between grain-supported and mud-supported texture. This discrimination was also reflected by the computed gamma-ray log, which recorded contributions from potassium and thorium and therefore assessed clay content reflected by fast relaxation times. A megaporosity class was equated with T2 relaxation times summed from 1024 to 2048 ms bins, and the volumetric curve compared favorably with variation over a range of vug sizes observed in the core. The complementary link between grain textures and pore textures was fruitful in the development of geomodels that integrates geologic core observations with petrophysical log measurements.

scholarly journals The Activity of Lipid A and Core Components of Bacterial Lipopolysaccharides in the Prevention of the Hypersensitive Response in Pepper

1997 ◽  
Vol 10 (7) ◽  
pp. 926-928 ◽  
Author(s):  
Mari-Anne Newman ◽  
Michael J. Daniels ◽  
J. Maxwell Dow
Keyword(s):  
Hypersensitive Response ◽  
Xanthomonas Campestris ◽  
Lipid A ◽  
Enteric Bacteria ◽  
Core Oligosaccharide ◽  
The Core ◽  
Minimal Structure ◽  
Core Components ◽  
Pre Treatment ◽  
Bacterial Lipopolysaccharides

Pre-treatment of leaves of pepper (Capsicum annuum) with lipopolysaccharide (LPS) preparations from enteric bacteria and Xanthomonas campestris could prevent the hypersensitive response caused by an avirulent X. campestris strain. By use of a range of deep-rough mutants, the minimal structure in Salmonella LPS responsible for the elicitation of this effect was determined to be lipid A attached to a disaccharide of 2-keto-3-deoxyoctulosonate; lipid A alone and the free core oligosaccharide from a Salmonella Ra mutant were not effective. For Xanthomonas, the core oligosaccharide alone had activity although lipid A was not effective. The results suggest that pepper cells can recognize different structures within bacterial LPS to trigger alterations in plant response to avirulent pathogens.

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