Production and properties of α-amylase fromBacillussp. BKL20

2010 ◽  
Vol 56 (4) ◽  
pp. 279-288 ◽  
Author(s):  
Olha I. Kubrak ◽  
Janet M. Storey ◽  
Kenneth B. Storey ◽  
Volodymyr I. Lushchak
Keyword(s):  
High Activity ◽  
Yeast Extract ◽  
Culture Medium ◽  
Metal Cations ◽  
Culture Conditions ◽  
Bacillus Sp ◽  
Good Resistance ◽  
Bivalent Metal ◽  
Maximum Production ◽  
Ph Range

As a result of screening Bacillus sp. strains isolated from different natural substrates, strain BKL20 was identified as a producer of a thermostable alkaline α-amylase. Maximum production of this α-amylase was achieved by optimizing culture conditions. Production of α-amylase seemed to be independent of the presence of starch in the culture medium and was stimulated by the presence of peptone (0.3%, m/v) and yeast extract (0.2%, m/v). The enzyme was thermostable and retained amylolytic activity after 30 min of incubation at 60 and 70 °C. High activity was maintained over a broad pH range, from 6.0 to 11.0, and the enzyme remained active after alkaline incubation for 24 h. Bacillus sp. BKL20 α-amylase was not stimulated by Ca2+or other bivalent metal cations and was not inhibited by EGTA or EDTA at 1–10 mmol/L, suggesting that this α-amylase is a Ca2+-independent enzyme. It also showed good resistance to both oxidizing (H2O2) and denaturating (urea) agents.

Adsorption Applications of Bacillus sp. Biomass for Decolorization of Soluble Reactive Dyes Contaminated Solutions

2011 ◽  
Vol 71-78 ◽  
pp. 2294-2297
Author(s):  
Yan Li Mao ◽  
Yun Yu ◽  
Shi Tian Luo ◽  
Xiao Lin Lv
Keyword(s):  
Culture Medium ◽  
Reactive Dyes ◽  
Culture Conditions ◽  
Inoculum Size ◽  
Bacillus Sp ◽  
Light Yellow ◽  
Molasses Wastewater ◽  
Decolorization Efficiency ◽  
Flocculating Activity ◽  
Initial Ph

The optimal conditions of production of biopolymer by the culture of Bacillus sp.were examined, using molasses wastewater to replace glucose as carbon source and energy source in the culture medium. Results showed that the COD concentration in molasses wastewater favorable for the growth of the Bacillus sp.was 4500 mg•L-1, and inoculum size of 6%(v/v), 28◦C, initial pH 7.0 and shaking speed of 160 r•min-1, under the optimal culture conditions, the highest flocculating activity achieved for Kaolin suspension was 97.5% and 4.0 g biomass /L broth was obtained. The Bacillus sp. biomass was effective in flocculating some soluble reactive dyes in aqueous solution, reactive Light-Yellow K-4G and reactive Turquoise Blue KN-G with a decolorization efficiency of 98.5 and 92.5%, respectively, using 20 mL of the flocculant in 500 mL of 100 mg•L-1 dye solution.

scholarly journals OPTIMIZATION OF CULTURE CONDITION FOR IAA RODUCTION BY BACILLUS SP. ISOLATED FROM CASSAVA FIELD OF VIETNAM

2021 ◽  
Vol 59 (3) ◽  
pp. 312
Author(s):  
Pham Viet Cuong ◽  
Nguyen Phuong Hoa
Keyword(s):  
16S Rrna ◽  
Yeast Extract ◽  
Culture Condition ◽  
Culture Conditions ◽  
Maximum Amount ◽  
Bacillus Sp ◽  
Rrna Sequence ◽  
16S Rrna Sequence ◽  
Iaa Production ◽  
Optimization Of Culture Conditions

Abstract. The bacteria capable producing IAA were isolated from roots of cassava growing in Tay Ninh region (Southern of Vietnam). The isolate producing highest amount of IAA was chosen for identification and optimization of culture conditions for IAA production. By analyzing 16S rRNA sequence, the selected isolate was identified as Bacillus sp. DTAN-M5. The influence of different parameters on IAA production of this strains was evaluated by “one factor at a time” method and the obtained results showed that the best conditions for IAA production of DTAN-M5 were medium with following components: 1% peptone; 0.5% sucrose , 0.6% yeast extract, 0.5% NaCl, pH 7.1, supplemented with 2mg/L L-tryptophan, incubation in a shaker 150 rpm at 30o C for 84 hours. The maximum amount of IAA was 84,13 µg/mL

Optimization of Culture Medium and Conditions for Bacillus coagulans T242 Producing Thermostable Lactase

2013 ◽  
Vol 634-638 ◽  
pp. 1259-1262 ◽  
Author(s):  
Fang Qian ◽  
Shu Juan Jiang ◽  
Tao Liu ◽  
Guang Qing Mu
Keyword(s):  
Optimum Condition ◽  
Liquid Medium ◽  
Yeast Extract ◽  
Culture Medium ◽  
Bacterial Culture ◽  
Bacillus Coagulans ◽  
Culture Conditions ◽  
Lactase Activity ◽  
Enhanced Production

A thermotolerant lactase-producing strain Bacillus coagulans T242 was obtained in our previous work, its media and culture conditions were investigated and optimized for enhanced production of lactase in the present work. Results showed medium containing 1.5% lactose, 1.0% peptone, 1.5% yeast extract, 0.7% MgSO4, and pH 8.0, was the optimum medium; And its optimum culture conditions were the age of bacterial culture 36 h, inoculation volume 2%, liquid medium volume loaded 30 mL/250 mL flask, 60 °C, 36 h. When cultured at the optimum condition Bacillus coagulans T242 yielded the maximum of 1.21U/mL lactase activity.

MICROBIOLOGICAL DEGRADATION OF QUERCITRIN

1963 ◽  
Vol 9 (2) ◽  
pp. 211-220 ◽  
Author(s):  
D. W. S. Westlake
Keyword(s):  
Carbon Monoxide ◽  
Carboxylic Acid ◽  
Aspergillus Flavus ◽  
Yeast Extract ◽  
Organic Nitrogen ◽  
Culture Medium ◽  
Alternative Pathway ◽  
Nitrogen Sources ◽  
Maximum Production ◽  
Aspergillus Flavus Group

A number of molds and bacteria were screened for their ability to degrade quercitrin. The molds, but not the bacteria, were particularly active and produced carbon monoxide. The degradation of quercitrin is dependent upon the synthesis of an inducible glycosidase (quercitrinase). This enzyme is synthesized by only a few members of the Aspergillus flavus group. Two of these strains synthesized quercitrinase and excreted it and other enzymes into the culture medium. Maximum production of quercitrinase was obtained with organic nitrogen sources such as yeast extract or phytone. Quercitrinase is induced by readily metabolized flavonols and flavonol-glycosides. The glycosidase is quite specific, liberating the rhamnose from the 3-position of quercitrin and myricitrin and the 7-position of robinin. The aglycone, quercetin, is subsequently metabolized to carbon monoxide and the depside of phloroglucinol-carboxylic acid and proto-catechuic acid. Evidence is also presented for an alternative pathway for the metabolism of the flavonol nucleus.

Optimization of Removal Conditions of Soluble Reactive Dyes from Aqueous Solutions by Bacillus sp. Biomass

2012 ◽  
Vol 430-432 ◽  
pp. 395-398
Author(s):  
Gai Xia Fang ◽  
Li Hua Chen
Keyword(s):  
Energy Source ◽  
Culture Medium ◽  
Reactive Dyes ◽  
Culture Conditions ◽  
Inoculum Size ◽  
Bacillus Sp ◽  
Optimal Conditions ◽  
Light Yellow ◽  
Decolorization Efficiency ◽  
Initial Ph

The optimal conditions of by the culture of Bacillus sp.were examined, using beer wastewater to replace glucose as carbon source and energy source in the culture medium. Results showed that the COD concentration in beer wastewater favorable for the growth of the Bacillus sp.was 5000 mg•L-1, and inoculum size of 5%(v/v), 30◦C, initial pH 7.0 and shaking speed of 150 r•min-1, under the optimal culture conditions, the Bacillus sp. biomass was effective in flocculating some soluble reactive dyes in aqueous solution, reactive Light-Yellow K-4G and reactive Turquoise Blue KN-G with a decolorization efficiency of 93.8 and 95.5%, respectively, using 20 mL of the culture medium in 500 mL of 100 mg•L-1 dye solution.

scholarly journals Design of a culture medium for optimal growth of the bacterium Pseudoxanthomonas indica H32 allowing its production as biopesticide and biofertilizer

AMB Express ◽  
2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Dayana Morales-Borrell ◽  
Nemecio González-Fernández ◽  
Néstor Mora-González ◽  
Carlos Pérez-Heredia ◽  
Ana Campal-Espinosa ◽  
...  
Keyword(s):  
Hydrogen Sulfide ◽  
Yeast Extract ◽  
Culture Medium ◽  
Nitrogen Sources ◽  
Optimal Growth ◽  
Culture Conditions ◽  
Medium Composition ◽  
Mineral Salts ◽  
Molar Ratios ◽  
Biotechnological Processes

Abstract Culture medium composition is one of the most important parameters to analyze in biotechnological processes with industrial purposes. The aim of this study was to design of a culture medium for optimal growth of the bacterium Pseudoxanthomonas indica H32 allowing its production as biopesticide and biofertilizer. The influence of several carbon and nitrogen sources and their molar ratios on P. indica H32 growth was investigated. The effect of different micronutrients such as mineral salts and vitamin on P. indica H32 growth was determined as well. A mixture design based on Design-Expert 10.0 Software was performed to optimize the culture medium concentration. Finally, in the designed medium, an attribute of the biological mechanism of action of the P. indica H32 against nematodes, was evaluated: the hydrogen sulfide production. It was found that tested carbon/nitrogen ratios were not a significant influence on P. indica H32 growth. Growth of P. indica H32 was favored with use of sucrose, yeast extract and phosphate buffer without the addition of any tested micronutrients. An optimal concentration of 10 g/L sucrose and 5 g/L yeast extract were obtained at a cost of 0.10 $/L. In this concentration, the specific growth rate (µ) and maximal optical density (Xmax) were equal to 0.439 h− 1 and 8.00 respectively. It was evidenced that under the culture conditions used, P. indica H32 produced hydrogen sulfide. The designed medium led to a 1.08 $/L reduction of costs in comparison to LB medium. These results were critical to carry on with biotechnological development of P. indica H32 as a bioproduct.

scholarly journals Optimisation of Culture Conditions for PLA-food-packaging Degradation by Bacillus sp. SNRUSA4

2021 ◽  
Vol 29 (1) ◽  
Author(s):  
Suwapha Sawiphak ◽  
Aroon Wongjiratthiti
Keyword(s):  
Weight Loss ◽  
Yeast Extract ◽  
Food Packaging ◽  
Ph Value ◽  
Culture Conditions ◽  
Bacillus Sp ◽  
Optimal Conditions ◽  
Extract Concentration ◽  
Degrading Bacteria ◽  
Initial Ph

Polylactic acid (PLA) is increasingly used in food-packaging production. The screening of PLA-food-packaging-degrading bacteria and optimisation of culture conditions for the PLA-food-packaging degradation by PLA-food-packaging-degrading bacteria were investigated for bioplastic waste management purposes. Only bacterial strain SNRUSA4 exhibited an increase in optical density (OD) in Basal Medium (BM) supplemented with 1.0 g/L of PLA-food-packaging as sole carbon source after 4 weeks of incubation. A weight loss of 7.3% and the rough and porous surface of PLA-food-packaging indicated that SNRUSA4 was a PLA-food-packaging-degrading bacterium. SNRUSA4 was able to degrade pure PLA which was confirmed from the clear zone formation around its colony on emulsified pure PLA agar plate. The 16S rRNA gene sequence of SNRUSA4 showed the similarity with thirteen Bacillus species. Hence, the strain SNRUSA4 was assigned as Bacillus sp. SNRUSA4. Response surface methodology with Box-Behnken Design was used to optimise the culture conditions including yeast extract concentration, initial pH value, temperature and agitation speed for growth and PLA-food-packaging degradation of Bacillus sp. SNRUSA4. The optimal conditions of Bacillus sp. SNRUSA4 was discovered in BM at initial pH value 7.02 with yeast extract concentration of 2.56% and agitated at 205.28 rpm at 31.68°C. Under optimal conditions, the OD of Bacillus sp. SNRUSA4 was up to 1.955, and the different OD between before and after optimisation was up to 1.752. Furthermore, the PLA-food-packaging weight loss also increased from 7.30% to 87.10% indicating that the PLA-food-packaging degradation under optimal conditions was higher than the unoptimised conditions. Therefore, Bacillus sp. SNRUSA4 is an efficient strain for degradation of PLA and PLA-food-packaging.

The influence of the initial concentrations of glucose and yeast extract on the ethanolic fermentation by Pachysolen tannophilus

1990 ◽  
Vol 55 (3) ◽  
pp. 854-866 ◽  
Author(s):  
Rodríguez V. Bravo ◽  
Rubio F. Camacho ◽  
Villasclaras S. Sánchez ◽  
Vico M. Castro
Keyword(s):  
Cell Growth ◽  
Ethanol Production ◽  
Yeast Extract ◽  
Culture Medium ◽  
Ethanolic Fermentation ◽  
Pachysolen Tannophilus ◽  
Significant Component ◽  
Batch Cultures

The ethanolic fermentation in batch cultures of Pachysolen tannophilus was studied experimentally varying the initial concentrations of two of the components in the culture medium: glucose between 0 and 200 g l-1 and yeast extract between 0 and 8 g l-1. The yeast extract appears to be a significant component both in cell growth and for ethanol production.

Production and Properties of a Bacterial Thermostable Exo-inulinase

2001 ◽  
Vol 56 (11-12) ◽  
pp. 1022-1028 ◽  
Author(s):  
Kristina Uzunova ◽  
Anna Vassileva ◽  
Margarita Kambourova ◽  
Viara Ivanova ◽  
Dimitrina Spasova ◽  
...  
Keyword(s):  
Enzyme Production ◽  
Enzyme Preparation ◽  
Batch Cultivation ◽  
Crude Enzyme ◽  
Growth Time ◽  
Bacillus Sp ◽  
High Thermostability ◽  
Crude Enzyme Preparation ◽  
Ph Range

Abstract Enzyme production of newly isolated thermophilic inulin-degrading Bacillus sp. 11 strain was studied by batch cultivation in a fermentor. The achieved inulinase and invertase activi­ ties after a short growth time (4.25 h) were similar or higher compared to those reported for other mesophilic aerobic or anaerobic thermophilic bacterial producers and yeasts. The investigated enzyme belonged to the exo-type inulinases and splitted-off inulin, sucrose and raffinose. It could be used at temperatures above 65 °C and pH range 5.5-7.5. The obtained crude enzyme preparation possessed high thermostability. The residual inulinase and inver­ tase activities were 92-98% after pretreatment at 65 °C for 60 min in the presence of substrate inulin.

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