scholarly journals Optimisation of Culture Conditions for PLA-food-packaging Degradation by Bacillus sp. SNRUSA4

2021 ◽  
Vol 29 (1) ◽  
Author(s):  
Suwapha Sawiphak ◽  
Aroon Wongjiratthiti
Keyword(s):  
Weight Loss ◽  
Yeast Extract ◽  
Food Packaging ◽  
Ph Value ◽  
Culture Conditions ◽  
Bacillus Sp ◽  
Optimal Conditions ◽  
Extract Concentration ◽  
Degrading Bacteria ◽  
Initial Ph

Polylactic acid (PLA) is increasingly used in food-packaging production. The screening of PLA-food-packaging-degrading bacteria and optimisation of culture conditions for the PLA-food-packaging degradation by PLA-food-packaging-degrading bacteria were investigated for bioplastic waste management purposes. Only bacterial strain SNRUSA4 exhibited an increase in optical density (OD) in Basal Medium (BM) supplemented with 1.0 g/L of PLA-food-packaging as sole carbon source after 4 weeks of incubation. A weight loss of 7.3% and the rough and porous surface of PLA-food-packaging indicated that SNRUSA4 was a PLA-food-packaging-degrading bacterium. SNRUSA4 was able to degrade pure PLA which was confirmed from the clear zone formation around its colony on emulsified pure PLA agar plate. The 16S rRNA gene sequence of SNRUSA4 showed the similarity with thirteen Bacillus species. Hence, the strain SNRUSA4 was assigned as Bacillus sp. SNRUSA4. Response surface methodology with Box-Behnken Design was used to optimise the culture conditions including yeast extract concentration, initial pH value, temperature and agitation speed for growth and PLA-food-packaging degradation of Bacillus sp. SNRUSA4. The optimal conditions of Bacillus sp. SNRUSA4 was discovered in BM at initial pH value 7.02 with yeast extract concentration of 2.56% and agitated at 205.28 rpm at 31.68°C. Under optimal conditions, the OD of Bacillus sp. SNRUSA4 was up to 1.955, and the different OD between before and after optimisation was up to 1.752. Furthermore, the PLA-food-packaging weight loss also increased from 7.30% to 87.10% indicating that the PLA-food-packaging degradation under optimal conditions was higher than the unoptimised conditions. Therefore, Bacillus sp. SNRUSA4 is an efficient strain for degradation of PLA and PLA-food-packaging.

scholarly journals Optimization of Culture Conditions for Amoxicillin Degrading Bacteria Screened from Pig Manure

2020 ◽  
Vol 17 (6) ◽  
pp. 1973 ◽  
Author(s):  
Xuanjiang Yang ◽  
Panpan Guo ◽  
Miao Li ◽  
Hualong Li ◽  
Zelin Hu ◽  
...  
Keyword(s):  
Response Surface ◽  
Ph Value ◽  
Culture Conditions ◽  
Pig Manure ◽  
Bacterial Strains ◽  
Inoculum Level ◽  
Fermentation Conditions ◽  
Culture Time ◽  
Degrading Bacteria ◽  
Initial Ph

(1) Objective: The objective of this study was to screen amoxicillin (AMX)-degrading bacterial strains in pig manure and optimize the fermentation conditions for these strains to achieve high fermentation rate, which can provide an effective way for the practical application of bacterial strains as antibiotic-degrading bacterial in treating livestock waste for antibiotic residues. (2) Methods: Antibiotic susceptibility tests and high-performance liquid chromatography tandem mass spectrometry (HPLC-MS/MS) were employed to screen AMX-degrading bacterial strains in pig manure. The culture conditions were optimized for AMX-degrading bacterial strains using Plackeet–Burman design (PBD), the steepest ascent design, and the response surface methods, coupled with the Box–Behnken design (BBD). The effects of culture time, temperature, rotator (mixing) speed, inoculum level, and initial pH value on the growth of AMX-degrading strains were investigated. Experimental data obtained from BBD were utilized to generate a second-order polynomial regression model for evaluating the effects of the tested variables on the optical density at 600 nm (OD600) of culture solutions as the growth indicator for the screened AMX-degrading strains. (3) Results: The initial pH, culture time, and the inoculum level had significant effects on the OD600 value (growth) of the screened AMX-degrading strains. The initial pH value was found to be the most critical factor influencing the growth of bacteria. The optimized culture condition for the bacterial growth determined by the response surface methodology was: the initial pH of 6.9, culture time of 52 h, and inoculum level of 2%. The average OD value of 12 different fermentation conditions in the initial fermentation tests in this study was 1.72 and the optimization resulted in an OD value of 3.00. The verification experiment resulted in an OD value of 2.94, which confirmed the adequacy of the optimization model for the determining the optimal culture condition. (4) Conclusions: The growth of the screened strain of AMX-degrading bacteria could be optimized by changing the fermentation conditions. The optimization could be achieved by using the Box–Behnken response surface method and Plackett–Burman experimental design.

Optimization of Removal Conditions of Soluble Reactive Dyes from Aqueous Solutions by Bacillus sp. Biomass

2012 ◽  
Vol 430-432 ◽  
pp. 395-398
Author(s):  
Gai Xia Fang ◽  
Li Hua Chen
Keyword(s):  
Energy Source ◽  
Culture Medium ◽  
Reactive Dyes ◽  
Culture Conditions ◽  
Inoculum Size ◽  
Bacillus Sp ◽  
Optimal Conditions ◽  
Light Yellow ◽  
Decolorization Efficiency ◽  
Initial Ph

The optimal conditions of by the culture of Bacillus sp.were examined, using beer wastewater to replace glucose as carbon source and energy source in the culture medium. Results showed that the COD concentration in beer wastewater favorable for the growth of the Bacillus sp.was 5000 mg•L-1, and inoculum size of 5%(v/v), 30◦C, initial pH 7.0 and shaking speed of 150 r•min-1, under the optimal culture conditions, the Bacillus sp. biomass was effective in flocculating some soluble reactive dyes in aqueous solution, reactive Light-Yellow K-4G and reactive Turquoise Blue KN-G with a decolorization efficiency of 93.8 and 95.5%, respectively, using 20 mL of the culture medium in 500 mL of 100 mg•L-1 dye solution.

Biodegradation of Swainsonine by Stenotrophomonas maltophilia Strain YLZZ-2 and its Isolation and Identification

2010 ◽  
Vol 178 ◽  
pp. 59-64
Author(s):  
Xing Hua Zhao ◽  
Xin He ◽  
Jian Hua Wang
Keyword(s):  
Stenotrophomonas Maltophilia ◽  
Sole Carbon Source ◽  
Degradation Rate ◽  
Ph Value ◽  
Culture Conditions ◽  
Isolation And Identification ◽  
16S Rdna Sequence ◽  
Degrading Bacteria ◽  
Initial Ph ◽  
Optimal Ph

Eight swainsonine (SW)-degrading bacteria were isolated from the soil where locoweed was buried for 6 months and one of the strains (YLZZ-2) was selected for further study. Based on morphology, physiologic tests, 16S rDNA sequence, and phylogenetic characteristics, the strain showed the greatest similarity to members of the order Stenotrophomonap and the closest to members of the Stenotrophomonas maltophilia group. The ability of the strain to degrade SW, as sole carbon source, was investigated under different culture conditions. The preferential temperature and initial pH value for the strain were 25～35 °C and 6.0～9.0, respectively. The optimal temperature for the strain was 30 °C and the optimal pH value was 7.0. There was positive correlation between degradation rate and inoculation amount. The growth of stain YLZZ-2 and degradation rate were fast, and YLZZ-2 could completely degradate 400 mg/L swainsoine within 24 h. There was a linear relationship between the growth of stain YLZZ-2 and degradation of swainsonine. These results highlight the potential of this bacterium to be used in detoxifying of SW in livestock consuming locoweed.

Adsorption Applications of Bacillus sp. Biomass for Decolorization of Soluble Reactive Dyes Contaminated Solutions

2011 ◽  
Vol 71-78 ◽  
pp. 2294-2297
Author(s):  
Yan Li Mao ◽  
Yun Yu ◽  
Shi Tian Luo ◽  
Xiao Lin Lv
Keyword(s):  
Culture Medium ◽  
Reactive Dyes ◽  
Culture Conditions ◽  
Inoculum Size ◽  
Bacillus Sp ◽  
Light Yellow ◽  
Molasses Wastewater ◽  
Decolorization Efficiency ◽  
Flocculating Activity ◽  
Initial Ph

The optimal conditions of production of biopolymer by the culture of Bacillus sp.were examined, using molasses wastewater to replace glucose as carbon source and energy source in the culture medium. Results showed that the COD concentration in molasses wastewater favorable for the growth of the Bacillus sp.was 4500 mg•L-1, and inoculum size of 6%(v/v), 28◦C, initial pH 7.0 and shaking speed of 160 r•min-1, under the optimal culture conditions, the highest flocculating activity achieved for Kaolin suspension was 97.5% and 4.0 g biomass /L broth was obtained. The Bacillus sp. biomass was effective in flocculating some soluble reactive dyes in aqueous solution, reactive Light-Yellow K-4G and reactive Turquoise Blue KN-G with a decolorization efficiency of 98.5 and 92.5%, respectively, using 20 mL of the flocculant in 500 mL of 100 mg•L-1 dye solution.

scholarly journals OPTIMIZATION OF CULTURE CONDITION FOR IAA RODUCTION BY BACILLUS SP. ISOLATED FROM CASSAVA FIELD OF VIETNAM

2021 ◽  
Vol 59 (3) ◽  
pp. 312
Author(s):  
Pham Viet Cuong ◽  
Nguyen Phuong Hoa
Keyword(s):  
16S Rrna ◽  
Yeast Extract ◽  
Culture Condition ◽  
Culture Conditions ◽  
Maximum Amount ◽  
Bacillus Sp ◽  
Rrna Sequence ◽  
16S Rrna Sequence ◽  
Iaa Production ◽  
Optimization Of Culture Conditions

Abstract. The bacteria capable producing IAA were isolated from roots of cassava growing in Tay Ninh region (Southern of Vietnam). The isolate producing highest amount of IAA was chosen for identification and optimization of culture conditions for IAA production. By analyzing 16S rRNA sequence, the selected isolate was identified as Bacillus sp. DTAN-M5. The influence of different parameters on IAA production of this strains was evaluated by “one factor at a time” method and the obtained results showed that the best conditions for IAA production of DTAN-M5 were medium with following components: 1% peptone; 0.5% sucrose , 0.6% yeast extract, 0.5% NaCl, pH 7.1, supplemented with 2mg/L L-tryptophan, incubation in a shaker 150 rpm at 30o C for 84 hours. The maximum amount of IAA was 84,13 µg/mL

scholarly journals Dioscorea alata as Alternative Culture Media for Fungal Cultivation and Biomass Production

2021 ◽  
Vol 44 (2) ◽  
Author(s):  
Suwapha Sawiphak ◽  
Aroon Wongjiratthiti ◽  
Chanankarn Saengprasan
Keyword(s):  
Biomass Production ◽  
Ph Value ◽  
Culture Media ◽  
Culture Conditions ◽  
Inoculum Size ◽  
Dioscorea Alata ◽  
Optimal Conditions ◽  
Potato Dextrose Broth ◽  
Fungal Cultivation ◽  
Purple Yam

Dioscorea alata (purple yam) is a tuber crop that contains plenty of nutrients. It is widely cultivated in Thailand, but it is underutilized. In this study, the suitability of purple yam to replace potato dextrose media for fungal growth was investigated. Mushrooms and molds were grown on purple yam dextrose agar (PYDA), whereas yeasts were cultured in purple yam dextrose broth (PYDB). Response surface methodology (RSM) with Box-Behnken design (BBD) was used to optimize the culture conditions for Saccharomyces cerevisiae biomass production. The growth profile of S. cerevisiae in PYDB under optimized culture conditions was also studied. All test mushrooms and molds recorded the highest colony diameter and mycelial dry weight on PYDA containing 40% purple yam. Similar to mushrooms and molds, yeasts in PYDB with 40% purple yam showed the highest number of cells. The growth of fungi on purple yam dextrose media was significantly higher than those on potato dextrose media under standard conditions. The optimal conditions from the RSM results for the biomass production of S. cerevisiae in PYDB were purple yam concentration of 49.61%, dextrose concentration of 4.87%, pH value of 5.74, and inoculum size of 7.00%. The biomass of S. cerevisiae in PYDB under the optimal conditions obtained from the results of the optimization by RSM was thirty times higher than S. cerevisiae biomass in potato dextrose broth under standard conditions. Our results suggest that purple yam could be an alternative to potato dextrose media for fungal cultivation.

scholarly journals Production of β-Glucosidase from a Newly IsolatedAspergillusSpecies Using Response Surface Methodology

2011 ◽  
Vol 2011 ◽  
pp. 1-9 ◽  
Author(s):  
Pilanee Vaithanomsat ◽  
Molnapat Songpim ◽  
Taweesiri Malapant ◽  
Akihiko Kosugi ◽  
Warunee Thanapase ◽  
...  
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Ammonium Sulfate ◽  
Yeast Extract ◽  
Culture Medium ◽  
Specific Activity ◽  
Ph Value ◽  
Sulfate Concentration ◽  
Extract Concentration ◽  
Fermentation Parameters

A newly isolated fungusAspergillus nigerSOI017 was shown to be a good producer of β-glucosidase from all isolated fungal strains. Fermentation condition (pH, cellobiose concentration, yeast extract concentration, and ammonium sulfate concentration) was optimized for producing the enzyme in shake flask cultures. Response surface methodology was used to investigate the effects of 4 fermentation parameters (yeast extract concentration, cellobiose concentration, ammonium sulfate concentration, and pH) on β-glucosidase enzyme production. Production of β-glucosidase was most sensitive to the culture medium, especially the nitrogen source yeast extract. The optimized medium for producing maximum β-glucosidase specific activity consisted of 0.275% yeast extract, 1.125% cellobiose, and 2.6% ammonium sulfate at a pH value of 3.

Screening of a Flocculant-Producing Strain and Optimal Conditions for Production of Bioflocculant

2011 ◽  
Vol 183-185 ◽  
pp. 829-833
Author(s):  
Jia Hong Wang ◽  
Jun Fei Wei ◽  
Hong Rui Ma
Keyword(s):  
Culture Conditions ◽  
Kaolin Clay ◽  
Optimal Conditions ◽  
Single Factor ◽  
Clay Suspension ◽  
Factors Affecting ◽  
Dilution Plate ◽  
Flocculating Activity ◽  
Initial Ph ◽  
High Possibility

A bioflocculant-producing strain M-3 with high flocculating activity was isolated from soil by conventional panel lineation and dilution-plate methods. It was preliminarily identified as Aspergillus based on its morphological and physiological characteristics of colony. The single factor experiments were conducted to optimize cultivating factors affecting flocculating activity, the optimal culture conditions were found to be as follows: glucose 10 g/L, glutamate 2 g/L, cultivating time 36 h, cultivating temperature 37 oC, shaking speed 120 r/min, initial pH 10. The strain could produce high effective flocculants with flocculating activity of 98.3% for kaolin clay suspension, which afforded high possibility of its practical use.

Removal of Silver (I) from Aqueous Solutions by Chitosan/Carbon Nanotube Nanocomposite Beads

2014 ◽  
Vol 893 ◽  
pp. 166-169 ◽  
Author(s):  
Walaikorn Nitayaphat ◽  
Thanut Jintakosol
Keyword(s):  
Adsorption Equilibrium ◽  
Ph Value ◽  
Dosage Level ◽  
Maximum Adsorption Capacity ◽  
Optimal Conditions ◽  
Adsorption Efficiency ◽  
Composite Adsorbent ◽  
Adsorbent Dosage ◽  
Composite Beads ◽  
Initial Ph

Chitosan/CNT nanocomposites were prepared by blending chitosan with carbon nanotubes (CNTs) and forming composite beads. The composites were used as Ag+adsorbents. Adsorption equilibrium experiments were carried out as a function of contact time, CNTs concentration, pH value, and adsorbent dosage level. The equilibrium time of Ag+adsorption was found to be 160 min. Composite adsorbent had the highest adsorption efficiency when the weight of CNTs was 0.01 wt%. The maximum Ag+removal took place at the initial pH value of 3. The optimum adsorbent dosage for Ag+removal was 5 g. Under above optimal conditions the maximum Ag+removal was 99.7%. The adsorption isotherm of chitosan/CNT nanocomposite bead agreed well with the Langmuir model. The maximum adsorption capacity was 0.393 mg/g.

Research on Flocculation Behavior in Diethyl Phthalate Removal from Water by Using Anionic Flocculants P(AM-AA-AMPS)

2013 ◽  
Vol 361-363 ◽  
pp. 726-729 ◽  
Author(s):  
Jiang Ya Ma ◽  
Huai Li Zheng ◽  
Xiao Min Tang ◽  
Wei Chen ◽  
Wen Wen Xue ◽  
...  
Keyword(s):  
Electron Microscope ◽  
Ph Value ◽  
Optimal Conditions ◽  
Single Factor ◽  
Initial Ph ◽  
Stirring Time ◽  
Flocculation Process ◽  
Scanning Electron ◽  
Significant Factors ◽  
Factor Technique

Flocculation process with P(AM-AA-AMPS) as flocculants was used for the DEP removal from water. The structure and morphologies of the flocculants was characterized by scanning electron microscope (SEM). To maximize the DEP removal efficiency, single factor technique and an orthogonal array L9 (34) were employed to optimize the factors of dosage, initial pH value and stirring time. The results show that the optimal conditions are dosage of 10mg·L-1, initial pH value of 9 and stirring time of 6min. Moreover, flocculants dosage is the best significant factors in DEP removal.

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