Optimization of Culture Medium and Conditions for Bacillus coagulans T242 Producing Thermostable Lactase

2013 ◽  
Vol 634-638 ◽  
pp. 1259-1262 ◽  
Author(s):  
Fang Qian ◽  
Shu Juan Jiang ◽  
Tao Liu ◽  
Guang Qing Mu
Keyword(s):  
Optimum Condition ◽  
Liquid Medium ◽  
Yeast Extract ◽  
Culture Medium ◽  
Bacterial Culture ◽  
Bacillus Coagulans ◽  
Culture Conditions ◽  
Lactase Activity ◽  
Enhanced Production

A thermotolerant lactase-producing strain Bacillus coagulans T242 was obtained in our previous work, its media and culture conditions were investigated and optimized for enhanced production of lactase in the present work. Results showed medium containing 1.5% lactose, 1.0% peptone, 1.5% yeast extract, 0.7% MgSO4, and pH 8.0, was the optimum medium; And its optimum culture conditions were the age of bacterial culture 36 h, inoculation volume 2%, liquid medium volume loaded 30 mL/250 mL flask, 60 °C, 36 h. When cultured at the optimum condition Bacillus coagulans T242 yielded the maximum of 1.21U/mL lactase activity.

Purification and Preliminary Characterization of a Thermostable Lactase from Bacillus coagulans T242

2013 ◽  
Vol 690-693 ◽  
pp. 1362-1365
Author(s):  
Shu Juan Jiang ◽  
Guang Qing Mu ◽  
Tao Liu ◽  
Fang Qian
Keyword(s):  
Optimum Condition ◽  
Molecular Mass ◽  
Bacillus Coagulans ◽  
Gel Permeation Chromatography ◽  
Lactase Activity ◽  
High Concentration ◽  
Preliminary Characterization ◽  
Sds Page ◽  
Gel Permeation

A thermostable lactase from Bacillus coagulans T242 was subjected to purification on DEAE chromatography followed by gel permeation chromatography, then the homogenous Bacillus coagulans T242-lactase was obtained, and its molecular mass was 55.0 kDa as shown in SDS-PAGE. Analysis indicated its optimum condition was 60 C and pH 6.8 and it was stable at 40~60 C and pH6.5~7.8; Mn2+, Mg2+ and Na+ at high concentration all had marked activation on lactase activity. Kinetic constants determination revealed Bacillus coagulans T242-lactase had a strong affinity for lactose.

Production and properties of α-amylase fromBacillussp. BKL20

2010 ◽  
Vol 56 (4) ◽  
pp. 279-288 ◽  
Author(s):  
Olha I. Kubrak ◽  
Janet M. Storey ◽  
Kenneth B. Storey ◽  
Volodymyr I. Lushchak
Keyword(s):  
High Activity ◽  
Yeast Extract ◽  
Culture Medium ◽  
Metal Cations ◽  
Culture Conditions ◽  
Bacillus Sp ◽  
Good Resistance ◽  
Bivalent Metal ◽  
Maximum Production ◽  
Ph Range

As a result of screening Bacillus sp. strains isolated from different natural substrates, strain BKL20 was identified as a producer of a thermostable alkaline α-amylase. Maximum production of this α-amylase was achieved by optimizing culture conditions. Production of α-amylase seemed to be independent of the presence of starch in the culture medium and was stimulated by the presence of peptone (0.3%, m/v) and yeast extract (0.2%, m/v). The enzyme was thermostable and retained amylolytic activity after 30 min of incubation at 60 and 70 °C. High activity was maintained over a broad pH range, from 6.0 to 11.0, and the enzyme remained active after alkaline incubation for 24 h. Bacillus sp. BKL20 α-amylase was not stimulated by Ca2+or other bivalent metal cations and was not inhibited by EGTA or EDTA at 1–10 mmol/L, suggesting that this α-amylase is a Ca2+-independent enzyme. It also showed good resistance to both oxidizing (H2O2) and denaturating (urea) agents.

scholarly journals Design of a culture medium for optimal growth of the bacterium Pseudoxanthomonas indica H32 allowing its production as biopesticide and biofertilizer

AMB Express ◽  
2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Dayana Morales-Borrell ◽  
Nemecio González-Fernández ◽  
Néstor Mora-González ◽  
Carlos Pérez-Heredia ◽  
Ana Campal-Espinosa ◽  
...  
Keyword(s):  
Hydrogen Sulfide ◽  
Yeast Extract ◽  
Culture Medium ◽  
Nitrogen Sources ◽  
Optimal Growth ◽  
Culture Conditions ◽  
Medium Composition ◽  
Mineral Salts ◽  
Molar Ratios ◽  
Biotechnological Processes

Abstract Culture medium composition is one of the most important parameters to analyze in biotechnological processes with industrial purposes. The aim of this study was to design of a culture medium for optimal growth of the bacterium Pseudoxanthomonas indica H32 allowing its production as biopesticide and biofertilizer. The influence of several carbon and nitrogen sources and their molar ratios on P. indica H32 growth was investigated. The effect of different micronutrients such as mineral salts and vitamin on P. indica H32 growth was determined as well. A mixture design based on Design-Expert 10.0 Software was performed to optimize the culture medium concentration. Finally, in the designed medium, an attribute of the biological mechanism of action of the P. indica H32 against nematodes, was evaluated: the hydrogen sulfide production. It was found that tested carbon/nitrogen ratios were not a significant influence on P. indica H32 growth. Growth of P. indica H32 was favored with use of sucrose, yeast extract and phosphate buffer without the addition of any tested micronutrients. An optimal concentration of 10 g/L sucrose and 5 g/L yeast extract were obtained at a cost of 0.10 $/L. In this concentration, the specific growth rate (µ) and maximal optical density (Xmax) were equal to 0.439 h− 1 and 8.00 respectively. It was evidenced that under the culture conditions used, P. indica H32 produced hydrogen sulfide. The designed medium led to a 1.08 $/L reduction of costs in comparison to LB medium. These results were critical to carry on with biotechnological development of P. indica H32 as a bioproduct.

Establishing Standard Protocols for Bacterial Culture in Biological Research in Canisters (BRIC) Hardware

2020 ◽  
Vol 4 (2) ◽  
pp. 58-69 ◽  
Author(s):  
Patricia Fajardo-Cavazos ◽  
Wayne L. Nicholson
Keyword(s):  
Gene Expression ◽  
Bacterial Culture ◽  
Culture Conditions ◽  
Media Culture ◽  
Ground Control ◽  
Biological Research ◽  
Confounding Factors ◽  
Cross Experiment ◽  
Spaceflight Experiments ◽  
Control Samples

AbstractThe NASA GeneLab Data System (GLDS) was recently developed to facilitate cross-experiment comparisons in order to understand the response of microorganisms to the human spaceflight environment. However, prior spaceflight experiments have been conducted using a wide variety of different hardware, media, culture conditions, and procedures. Such confounding factors could potentially mask true differences in gene expression between spaceflight and ground control samples. In an attempt to mitigate such confounding factors, we describe here the development of a standardized set of hardware, media, and protocols for liquid cultivation of microbes in Biological Research in Canisters (BRIC) spaceflight hardware, using the model bacteria Bacillus subtilis strain 168 and Staphylococcus aureus strain UAMS-1 as examples.

The influence of the initial concentrations of glucose and yeast extract on the ethanolic fermentation by Pachysolen tannophilus

1990 ◽  
Vol 55 (3) ◽  
pp. 854-866 ◽  
Author(s):  
Rodríguez V. Bravo ◽  
Rubio F. Camacho ◽  
Villasclaras S. Sánchez ◽  
Vico M. Castro
Keyword(s):  
Cell Growth ◽  
Ethanol Production ◽  
Yeast Extract ◽  
Culture Medium ◽  
Ethanolic Fermentation ◽  
Pachysolen Tannophilus ◽  
Significant Component ◽  
Batch Cultures

The ethanolic fermentation in batch cultures of Pachysolen tannophilus was studied experimentally varying the initial concentrations of two of the components in the culture medium: glucose between 0 and 200 g l-1 and yeast extract between 0 and 8 g l-1. The yeast extract appears to be a significant component both in cell growth and for ethanol production.

Study on Optimization of Fermentation Condition for Nitrilase-Producer Escherichia Coli BL21 (DE3)/pET-Nit

2011 ◽  
Vol 175-176 ◽  
pp. 192-196 ◽  
Author(s):  
Li Li Feng ◽  
Jian Fei Zhang ◽  
Hui Luo ◽  
Zheng Li ◽  
Hong Jie Zhang
Keyword(s):  
Escherichia Coli ◽  
Recombinant Strain ◽  
Culture Medium ◽  
Culture Conditions ◽  
Fermentation Condition ◽  
Hydrogen Phosphate ◽  
Strain Bl21 ◽  
Initial Ph ◽  
Potassium Hydrogen ◽  
Optimization Of Fermentation

The paper concentrated on the optimization of the recombinant strain BL21 (DE3)-PE7-Nit. The component of culture medium and the culture conditions were optimized. The optimized medium was: yeast extract 10 g/l, L-glutamate sodium 8 g/l, MgSO4.7H2O 0.7 g/l, Isopropyl-β-D-thiogalactopyranoside 0.3 mmol/L, potassium hydrogen phosphate 0.5 g / L, phosphate Potassium 0.5 g / L and the culture condition was: initial pH 7.0, inoculum 2%. The result showed that the activity of nitrilase prepared with these conditions increased by 130.37 % through optimization.

scholarly journals Sensitive and Selective Culture Medium for Detection of Environmental Clostridium difficile Isolates without Requirement for Anaerobic Culture Conditions

2014 ◽  
Vol 52 (9) ◽  
pp. 3259-3263 ◽  
Author(s):  
Jennifer L. Cadnum ◽  
Kelly N. Hurless ◽  
Abhishek Deshpande ◽  
Michelle M. Nerandzic ◽  
Sirisha Kundrapu ◽  
...  
Keyword(s):  
Clostridium Difficile ◽  
Culture Medium ◽  
Culture Conditions ◽  
Anaerobic Culture ◽  
Selective Culture Medium

Inhibition of proliferative growth in glioma cells by calmodulin antagonists

1986 ◽  
Vol 65 (1) ◽  
pp. 74-79 ◽  
Author(s):  
Nobuyuki Suzuki ◽  
Tetsuo Kanno ◽  
Yutaka Nagata ◽  
Taiji Kato
Keyword(s):  
Culture Medium ◽  
Deoxyribonucleic Acid ◽  
Control Level ◽  
Glioma Cells ◽  
Culture Conditions ◽  
Calmodulin Antagonists ◽  
High Concentration ◽  
Content Type ◽  
Chemically Induced ◽  
Proliferative Growth

✓ The effects of several calmodulin antagonists, such as N-(6-aminohexyl)-5-chloro-1-naphthalene-sulfonamide hydrochloride (W-7) and its dechlorinated structural analogue (W-5), on the growth and proliferation of cultured and transplanted glioma (GA-1, chemically induced from rat glioblasts) were evaluated. Under culture conditions, the concentration of W-7 necessary to exert 50% inhibition of GA-1 glioma cell growth was 50 µM. However, W-5, with a lower binding affinity to calmodulin than W-7, caused no definite inhibition of the proliferation of GA-1 cells in culture. When a low concentration of W-7 (12.5 µM) was added to the culture medium, deoxyribonucleic acid (DNA) synthesis in the GA-1 glioma cells was not markedly affected, whereas both ribonucleic acid (RNA) and protein syntheses were strongly suppressed on incubation for 24 hours. When a high concentration of W-7 (25.0 to 75.0 µM) was applied to the medium, synthesis of DNA, RNA, and protein was distinctly inhibited. When W-7 (50.0 µM) was added to the incubation medium, the calmodulin concentration in the cultured GA-1 was reduced to as much as half the control level within 2 hours, and thereafter remained at this level. Whereas control rats intraperitoneally transplanted with GA-1 cells could survive for 14 to 21 days, daily intraperitoneal injections of W-7 at concentrations of 1.0, 3.0, and 10.0 mg/kg body weight prolonged the survival span to between 21 and 26 days; this corresponded to an increased life span of about 40% compared to the controls.

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