Effects of annual crown pruning and serial propagation on rooting of stem cuttings from Douglas-fir

1983 ◽  
Vol 13 (3) ◽  
pp. 419-424 ◽  
Author(s):  
Donald L. Copes
Keyword(s):  
Douglas Fir ◽  
Clonal Variation ◽  
Stem Cuttings ◽  
Rooting Of Cuttings ◽  
Serial Propagation

Success of rooting from stem cuttings of 14 Douglas-fir (Pseudotsugamenziesii (Mirb.) Franco) clones increased significantly from 42% in 1974 to 67% in 1981. The increase was slightly more than 3% each year despite ortet age increasing from 10 and 13 years in 1974 to 17 and 20 years in 1981. The greatest increase occurred from the 3rd through the 6th year of annual rooting trials. Yearly pruning of ortets and rooted ramets of the same clones is thought to have reinvigorated shoots from the pruned crowns of trees. Several annual prunings seemed to be required before average rooting potential increased markedly. No translocation of the reinvigoration stimulus from the pruned to the adjacent unpruned areas of the same trees was detected. Rooting of cuttings from ortets from pruned areas was at least 9% more than cuttings from unpruned areas. No additional invigoration from serial propagation of cuttings was noted for rooting potential, but such cuttings grew a little taller and slightly less plagiotropic 1 year after rooting than were ramets propagated from pruned ortets. Significant clonal variation was found with all treatments. Evidence of a change in physiological vigor of the ortets and rooted ramets was externally visible in the formation of abundant lammas shoots on pruned areas of the 17- and 20-year-old ortets and rooted ramets. Abundant lammas growth is normally found only on Douglas-fir trees less than 10 years old.

Effects of long-term pruning, meristem origin, and branch order on the rooting of Douglas-fir stem cuttings

1992 ◽  
Vol 22 (12) ◽  
pp. 1888-1894 ◽  
Author(s):  
Donald L. Copes
Keyword(s):  
Flat Surface ◽  
Tree Height ◽  
Douglas Fir ◽  
Second Order ◽  
Stem Cuttings ◽  
First Order ◽  
Secondary Origin ◽  
Rooting Of Cuttings ◽  
Better Than

The rooting percentages of 14 Douglas-fir (Pseudotsugamenziesii (Mirb.) Franco) clones were examined annually from 1974 to 1988. The trees were 10 and 13 years old in 1974 and were pruned to 2.0 m in 1978 and 1979 and then recut annually to 0.5, 1.0, or 1.5 m, starting in 1983. The pruned trees showed no evidence of decreased rooting percentage even after 15 years; average rooting increased from 47% in 1974 to 74% in 1986. Rooting percentage was significantly influenced by tree height. Cuttings collected from 0.5 m tall ramets exhibited better rooting than cuttings from 1.0 or 2.0 m tall ramets, and cuttings from 1.0 m tall ramets rooted better than cuttings from 2.0 m tall ramets. Rooting of cuttings collected from 0.5 m high subinterval zones within trees showed a negative linear relation between rooting percentage and collection height. Cuttings collected from the 0–0.5 m zone rooted 25% better than cuttings from the 1.5–2.0 m zone of the 2 m tall trees. That difference was significant at p < 0.05. A test of rooting of larger, more orthotropic cuttings gathered from the upper flat surface of pruned ramets indicated that the cuttings from the top rooted significantly less than smaller, more plagiotropic cuttings from the contiguous side areas (24 versus 33%, respectively). Rooting comparisons of meristems of primary and secondary origin showed significantly greater rooting of secondary meristems. Comparison of rooting of second-order and first-order meristems of secondary origin indicated the second-order twigs averaged 26% better rooting than the first-order branch tips when the cuttings were collected in January and placed in the rooting beds in February.

scholarly journals VEGETATIVE RESCUE AND ROOTING OF CUTTINGS OF DIFFERENT STOCK PLANTS OF Sequoia sempervirens

CERNE ◽  
2017 ◽  
Vol 23 (4) ◽  
pp. 435-444 ◽  
Author(s):  
Mariane de Oliveira Pereira ◽  
Alessandro Camargo Ângelo ◽  
Marcio Carlos Navroski ◽  
Mario Dobner Júnior ◽  
Luciana Magda de Oliveira
Keyword(s):  
Genetic Effect ◽  
Sequoia Sempervirens ◽  
Stem Cuttings ◽  
Fast Growing ◽  
Rooting Of Cuttings ◽  
Living Species ◽  
Recovered Material ◽  
Over 40 Years ◽  
Number Of Shoots

ABSTRACT Sequoia is a fast-growing, long-living species, producing durable timber. The aim of this study was to test different methods for the vegetative rescue of Sequoia sempervirens trees over 40 years old, made at different periods of the year, and, later, testing the rooting of individualized cuttings in planned arrays. Twenty-four individual sequoias were rescued, applying girdling and semi-girdling at three different heights (-10, 0, and 30 cm). The first collection was made 90 days after application of the treatments, being repeated at 150, 240, and 360 days. The percentage of budding trees and the number of shoots per array were registered. In all collections, shoots produced cuttings, which were placed for rooting in mini-tunnels. Cutting survival (%), rooting (%), and number of roots were registered, per array, and per collection. The species vegetative rescue proved to be efficient for the production of shoots for stem cuttings, especially with girdling at 30 cm and semi-girdling at -10 cm. However, it is difficult to define the best method, mainly because of the genetic effect among stock plants. The rooting of cuttings, of recovered material, presented good results (average >65%), also with great differences among stock plants. The potential for rooting of cuttings varied according to different planting periods, with high rooting rates in all seasons, especially in summer. Sequoia sempervirens shows the potential for vegetative rescue and cloning by rooting of cuttings, and this may lead to new studies, with a view towards fixing clones.

scholarly journals   Alanine conjugate of indole-3-butyric acid improves rooting of highbush blueberries

2012 ◽  
Vol 58 (No. 5) ◽  
pp. 236-241 ◽  
Author(s):  
S. Mihaljević ◽  
B. Salopek-Sondi
Keyword(s):  
Butyric Acid ◽  
Vaccinium Corymbosum ◽  
Highbush Blueberry ◽  
Stem Cuttings ◽  
Practical Application ◽  
Rooting Of Cuttings ◽  
Highbush Blueberries ◽  
Set Up ◽  
Indole 3 Acetic Acid

Auxins and their synthetic analogues are commonly used for rooting of cuttings, but their efficiency depends on experimental set-up and, even more importantly, on species or cultivar, and type of explants investigated. In attempt to improve rooting procedure for highbush blueberries (Vaccinium corymbosum L.), we investigated alanine conjugate of indole-3-butyric acid (IBA-Ala) as potential root-promoting compound and compared with commonly used auxins indole-3-butyric acid (IBA) and indole-3-acetic acid (IAA). The effect of different concentrations of auxins on the rooting of highbush blueberry stem cuttings (var. Bluecrop, Bluetta, Burlington and Jersey) and in vitro-derived microcuttings (var. Jersey) was investigated. Auxin treatments significantly promoted rooting of all four varieties in comparison to control. The most efficient rooting promoter in all varieties appeared IBA-Ala (provided up to 83%, and 93% of the rooted cuttings and microcuttings, respectively). Furthermore, IBA-Ala caused a vigorous, well-branched root system that resulted in better acclimatization and survival of plants. Results suggest the application of IBA-Ala may be beneficial for rooting of difficult-to-root varieties of blueberry and related species and their practical application in the nursery industry. &nbsp;

Clonal variation in pollen-related characteristics of Douglas-fir

1982 ◽  
Vol 12 (2) ◽  
pp. 403-408 ◽  
Author(s):  
W. T. Adams
Keyword(s):  
Genetic Variation ◽  
Pollen Grains ◽  
Seed Orchard ◽  
Douglas Fir ◽  
Clonal Variation ◽  
Pollen Production ◽  
The Mean ◽  
Pollen Cone ◽  
Grain Diameter

The mean number of pollen grains produced per pollen cone (P) ranged from 37 310 to 62 960 ([Formula: see text], SE = 1584) among eight seed-orchard clones of Douglas-fir (Pseudotsugamenziesii (Mirb.) Franco) and correlated significantly (r = 0.87, p < 0.05) with the mean number of microsporophylls per cone but not significantly with pollen cone length. The number of pollen grains per milligram (G) also differed among clones (range 2323–3112; [Formula: see text], SE = 32) and correlated significantly and negatively (r = −0.86) with grain diameter. Although this study provides evidence for genetic variation in both P and G, the differences were relatively small. Adjusting pollen-cone counts for P would add little, if any, precision to estimates of pollen production in seed-orchard clones. If balanced clonal representation in pollen mixes is desired, equal weights of pollen would probably suffice for most breeding applications.

scholarly journals Comparison of Rootability of Stem Cuttings from Seedlings of Aesculus sp. and Mature Aesculus × arnoldiana ‘Autumn Splendor’

1988 ◽  
Vol 6 (2) ◽  
pp. 69-71
Author(s):  
B.A. Bergmann ◽  
W.P. Hackett ◽  
H. Pellett
Keyword(s):  
Butyric Acid ◽  
Developmental Stages ◽  
Stem Cuttings ◽  
Mature Tree ◽  
Mature Trees ◽  
Old Growth ◽  
Rooting Of Cuttings ◽  
Root Suckers ◽  
Stem Segments

Rootability was evaluated for stem cuttings taken at varying positions and developmental stages from seedlings of Aesculus and from the mature trees of Aesculus × arnoldiana ‘Autumn Splendor’. Rooting was 100% for cuttings taken from 2-week-old seedlings and 0% for those obtained from the crown of the mature tree 2 months after commencement of spring growth flush. Intermediate rootability was found for cuttings taken from 1-month-old seedlings and from the 2 week-old growth of suckers at the base of the mature tree. Rooting of cuttings with intermediate rooting potential was increased by a quick dip treatment with 2500 ppm indole-3-butyric acid (IBA) or by using apical rather than basal stem segments. Commercially acceptable rooting was not achieved using cuttings from root suckers or the crown of Aesculus ‘Augumn Splendor’.

scholarly journals 567 PB 006 AUXIN SENSITIVITY OF DOUGLAS-FIR STEM CUTTINGS

HortScience ◽  
1994 ◽  
Vol 29 (5) ◽  
pp. 513a-513
Author(s):  
William M. Proebsting
Keyword(s):  
Douglas Fir ◽  
The Other ◽  
Tree Plantations ◽  
Christmas Tree ◽  
Stem Cuttings ◽  
Wide Range ◽  
Better Than

Douglas-fir clones have a wide range of rooting potential, but the species is generally considered difficult to root. We have reported previously that NAA is approximately 14-times more active than IBA in the clones tested, with an optimum of about 5 to 10 mM NAA. In contrast, other programs routinely use about 25 mM IBA to propagate Douglas-fir cuttings, a concentration that is relatively inactive in our clones. To address questions raised by these observations, we have incorporated auxin treatments into our long term program to select Douglas-fir clones with high rooting potential. We collect 20 cuttings of each clone identified in Christmas tree plantations, and retain clones rooting ≥ 80%. Beginning in 1991, we treated 10 cuttings of each clone with 5 mM NAA, the other 10 cuttings with 25 mM IBA. Over three years, 1158 clones received the split treatments. Of 222 clones rooting ≥ 80%) approximately half rooted ≥ 80% in response to NAA only. The remainder either responded to IBA or to both NAA and IBA. These results support our previous observations that NAA stimulates rooting of Douglas-fir better than IBA. However, they also suggest that there may be clones sensitive to IBA or to both NAA and IBA.

scholarly journals SHOOT POSITION, IBA AND AGROBACTERIUM RHIZOGENES INFLUENCE PROPAGATION OF HAZELNUT, CORYLUS AVELLANA

HortScience ◽  
1990 ◽  
Vol 25 (9) ◽  
pp. 1150g-1150
Author(s):  
William M. Proebsting ◽  
Nahla V. Bassil ◽  
David A. Lightfoot
Keyword(s):  
Genetic Transformation ◽  
Agrobacterium Rhizogenes ◽  
Corylus Avellana ◽  
Root Initiation ◽  
Stem Cuttings ◽  
Influence Propagation ◽  
Rooting Of Cuttings ◽  
Shoot Position

Propagation of Corylus avellana stem cuttings may be limited by either root initiation or bud abscission. We divided juvenile shoots of 3 varieties growing in layering beds in mid-July into 4 or 5 3-node cuttings with leaves at the upper two nodes, except that terminal cuttings had one expanded leaf. Cuttings were treated with 5 mM IBA in 50% EtOH, a mixture of A. rhizogenes strains A7 + 22 or left untreated. IBA and bacteria stimulated rooting of cuttings from all shoot positions. Rooting of the terminal cuttings (<50%) was less than that of the sub-terminal cuttings (>80%). Bud retention was <50% on terminal cuttings, nearly 100% on sub-terminal cuttings. Using juvenile stock plants of various varieties, sub-terminal cuttings treated with Agrobacterium or 5 mM IBA may yield 70-90% cuttings with both roots and buds, Agravitropic roots, characteristic of genetic transformation, were observed on Agrobacterium-treated cuttings. Dot blots probed for TL-DNA were negative, however.

Callus Formation and Root Initiation in Stem Cuttings of Douglas-fir (Pseudotsugamenziesii (Mirb.) Franco)

1972 ◽  
Vol 2 (2) ◽  
pp. 121-134 ◽  
Author(s):  
J. C. Heaman ◽  
J. N. Owens
Keyword(s):  
Callus Formation ◽  
Douglas Fir ◽  
Vascular Cambium ◽  
Complex Pattern ◽  
Root Initiation ◽  
Stem Cuttings ◽  
Root Primordia ◽  
Weekly Basis ◽  
Vascular Tissues ◽  
Rooting Response

Changes taking place at the base of stem cuttings of Douglas-fir during callus formation and root initiation are described. Material was taken from nine sources, including trees of different ages and with different histories of rooting response. Gross morphology and anatomy of the cutting bases are described using serial longitudinal sections of samples taken on a weekly basis over a 4-month period. The basal callus proliferated from the lowest cells of the vascular cambium and a complex pattern of differentiating vascular tissues arose within the callus mass. The root primordia arose in this callus in association with the differentiating phloem and wound cambium. Variation in callus formation and root initiation is described both within and between clones.

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