scholarly journals   Alanine conjugate of indole-3-butyric acid improves rooting of highbush blueberries

2012 ◽  
Vol 58 (No. 5) ◽  
pp. 236-241 ◽  
Author(s):  
S. Mihaljević ◽  
B. Salopek-Sondi
Keyword(s):  
Butyric Acid ◽  
Vaccinium Corymbosum ◽  
Highbush Blueberry ◽  
Stem Cuttings ◽  
Practical Application ◽  
Rooting Of Cuttings ◽  
Highbush Blueberries ◽  
Set Up ◽  
Indole 3 Acetic Acid

Auxins and their synthetic analogues are commonly used for rooting of cuttings, but their efficiency depends on experimental set-up and, even more importantly, on species or cultivar, and type of explants investigated. In attempt to improve rooting procedure for highbush blueberries (Vaccinium corymbosum L.), we investigated alanine conjugate of indole-3-butyric acid (IBA-Ala) as potential root-promoting compound and compared with commonly used auxins indole-3-butyric acid (IBA) and indole-3-acetic acid (IAA). The effect of different concentrations of auxins on the rooting of highbush blueberry stem cuttings (var. Bluecrop, Bluetta, Burlington and Jersey) and in vitro-derived microcuttings (var. Jersey) was investigated. Auxin treatments significantly promoted rooting of all four varieties in comparison to control. The most efficient rooting promoter in all varieties appeared IBA-Ala (provided up to 83%, and 93% of the rooted cuttings and microcuttings, respectively). Furthermore, IBA-Ala caused a vigorous, well-branched root system that resulted in better acclimatization and survival of plants. Results suggest the application of IBA-Ala may be beneficial for rooting of difficult-to-root varieties of blueberry and related species and their practical application in the nursery industry.  

scholarly journals Regeneration ability of different cultivars of the highbush blueberry (Vaccinium corymbosum L.) propagated by green stem cuttings

2019 ◽  
Vol 64 (3) ◽  
pp. 319-325
Author(s):  
N. B. Pavlovskiy
Keyword(s):  
Vaccinium Corymbosum ◽  
Regeneration Ability ◽  
Highbush Blueberry ◽  
Stem Cuttings ◽  
Regenerative Capacity ◽  
Biometric Parameters ◽  
Planting Material ◽  
Highbush Blueberries ◽  
Genetically Determined ◽  
Degrees Of Severity

An assessment of the regenerative capacity was made for 20 highbush blueberry cultivars and 3 half-highbush blueberry cultivars introduced in Belarus, based on 9-year research results. Has been established that the regeneration ability of the green cuttings of blueberry is genetically determined with varying degrees of severity in one or another cultivar. The results obtained allowed to classify blueberry cultivars according to their root-forming ability into three groups: easily rooted ((‘Elizabeth’, ‘Hardyblue’, ‘Northland’), medium rooted ((‘Blueray’, ‘Coville’, ‘Croatan’, ‘Darrow’, ‘Denise Blue’, ‘Herbert’, ‘Jersey’, ‘Northcountry’, ‘Reka’, ‘Rubel’) and difficult to resist (‘Bluecrop’, ‘Bluetta’, ‘Bluerose’, ‘Carolinablue’, ‘Duke’, ‘Earliblue’, ‘Nelson’, ‘Northblue’, ‘Patriot’, ‘Weymouth’). It is reliably shown that one hundred varieties of this crop, possessing a higher rootforming ability, produce plants with large biometric parameters of the above-ground sphere. The blue cuttings of highbush and half-highbush blueberries have a satisfactory regenerative capacity, which makes it possible to use this method of vegetative propagation for replication and to obtain planting material of this culture in Belarus.

scholarly journals Modification of Soil Properties for Blueberry (Vaccinium Corymbosum L.) Cultivation

2013 ◽  
Vol 30 (1) ◽  
pp. 1-10
Author(s):  
Jana Apse ◽  
Aldis Karklins
Keyword(s):  
Soil Properties ◽  
Root Zone ◽  
Sandy Loam ◽  
Soil Bulk Density ◽  
Vaccinium Corymbosum ◽  
Soil Reaction ◽  
Highbush Blueberry ◽  
Highbush Blueberries ◽  
Set Up ◽  
Experimental Plots

Abstract Highbush blueberries are a relatively new crop in Latvia raising interest in farmers who wish to diversify their commercial horticultural activities. It is considered that soil properties are one of the possible shortcomings limiting establishment of high-productive plantations in areas where soil reaction is close to neutral. The research objective was to test the main soil characteristics in a commercial highbush blueberry plantation where initial soil properties were changed as a result of soil modification (deep tillage, peat additions) before planting of bushes and afterwards maintained (mulching with acid peat and sawdust) periodically. The research was carried out in 2011 - in a farm where the blueberry plantation had been established seven years ago. The experimental plots were arranged on a complex slope, by choosing different parts of terrain. Soil - Haplic Cambisol, sandy loam, formed on a low calcareous moraine. Original topsoil’s reaction was pH KCl 5.37. Five experimental plots, each consisting of seven bushes, were set up. Research showed that modification of topsoil significantly changed the physical and agrochemical properties of soil. Soil bulk density reduced, porosity and soil aeration increased. Sphagnum peat (pH KCl 3.0) as a soil modifier allowed efficiently reducing the soil reaction at the soil root zone and provided an optimal environment for blueberries. Soil properties were not the determinant factor that limited establishment of a productive blueberry plantation. Soil properties adjusted accordingly to the requirements of highbush blueberry gave the possibility of establishing plantations also in typical mineral soils that develop on a low calcareous moraine.

scholarly journals The Effect of Biostimulators and Indole-3-Butyric Acid on Rooting of Stem Cuttings of Two Ground Cover Roses

2020 ◽  
Vol 73 (1) ◽  
Author(s):  
Andrzej Pacholczak ◽  
Karolina Nowakowska
Keyword(s):  
Butyric Acid ◽  
Free Amino ◽  
Water Solution ◽  
Soluble Sugars ◽  
Ground Cover ◽  
Stem Cuttings ◽  
Synthetic Auxin ◽  
Rooting Of Cuttings ◽  
Amino Acid Contents

One of the important groups of roses are ground cover roses, used not only as garden plants but also in urban green areas for covering scarps and hill slopes. Roses are mostly propagated in vitro and by budding, grafting, and cuttings however, the latter is feasible only in certain rose groups. To hasten the production of plant material, preparations that stimulate rhizogenesis are used, which are based mainly on auxins and enhance the growth of adventitious roots in cuttings. Other substances capable of stimulating plant growth are biostimulators, which can be an alternative to rooting formulations containing auxins. The goal of this experiment was to compare the effects of the biostimulators AlgaminoPlant and Goteo with that of the synthetic auxin indole-3-butyric acid (IBA) on rooting of cuttings in two ground cover roses Elfrid (‘Kormuse’) and Weisse Immensee (‘Korweirim’). In the preparations, IBA in the form of Rhizopon (1% IBA) or water solution (200 mg L<sup>−1</sup>) was used, whereas the biostimulators were applied at the concentration of 0.2% for foliage spraying. Both biostimulators positively affected the percentage of rooted cuttings, whereas their effect on the degree of rooting was comparable or slightly weaker than that of synthetic auxin. Both biopreparations stimulated the growth of new shoots in cuttings. The use of Goteo resulted in increased content of chlorophyll and total soluble sugars in cuttings, whereas levels of free amino acids and polyphenolic acids were decreased. The synthetic auxin IBA increased the total sugar concentration and the free amino acid contents in cuttings were decreased.

scholarly journals In Vitro Culture of Heuchera villosa ‘Caramel’

HortScience ◽  
2017 ◽  
Vol 52 (4) ◽  
pp. 622-624 ◽  
Author(s):  
Hua Q. Zhao ◽  
Qing H. He ◽  
Li L. Song ◽  
Mei F. Hou ◽  
Zhi G. Zhang
Keyword(s):  
Acetic Acid ◽  
In Vitro Culture ◽  
Butyric Acid ◽  
Shoot Regeneration ◽  
Ms Medium ◽  
Shoot Induction ◽  
Induction Rate ◽  
Regenerated Plantlets ◽  
Indole 3 Acetic Acid

The procedure for Heuchera villosa ‘Caramel’ propagation was investigated, which involves shoot regeneration, rooting of regenerated shoots, and acclimation of regenerated plantlets. Petioles, as explants, were cultured on MS medium supplemented with 1-naphthylacetic acid (NAA), benzylaminopurine (BA), thidiazuron (TDZ) and callus formed on all media. Shoots were observed to proliferate from callus on media with BA and NAA, whereas no shoots regenerated on media with TDZ and NAA. On media containing 0.5 or 1.0 mg·L−1 BA in combination with NAA, the regenerated shoots showed severe hyperhydricity, whereas on media containing 0.1 mg·L−1 BA in combination with NAA, the regenerated shoots grew normally. The highest shoot induction rate, 90.6%, was obtained on media containing 0.1 mg·L−1 BA and 0.01 mg·L−1 NAA. The effects of indole-3-acetic acid (IAA), indole-3-butyric acid (IBA), and NAA on rooting of H. villosa ‘Caramel’ was explored. The highest rooting rate (95%) was obtained on 1/2 MS medium containing 0.2 mg·L−1 NAA. In the subsequent acclimation experiments, about 85% of rooted plantlets survived and grew normally.

scholarly journals Comparison of Rootability of Stem Cuttings from Seedlings of Aesculus sp. and Mature Aesculus × arnoldiana ‘Autumn Splendor’

1988 ◽  
Vol 6 (2) ◽  
pp. 69-71
Author(s):  
B.A. Bergmann ◽  
W.P. Hackett ◽  
H. Pellett
Keyword(s):  
Butyric Acid ◽  
Developmental Stages ◽  
Stem Cuttings ◽  
Mature Tree ◽  
Mature Trees ◽  
Old Growth ◽  
Rooting Of Cuttings ◽  
Root Suckers ◽  
Stem Segments

Rootability was evaluated for stem cuttings taken at varying positions and developmental stages from seedlings of Aesculus and from the mature trees of Aesculus × arnoldiana ‘Autumn Splendor’. Rooting was 100% for cuttings taken from 2-week-old seedlings and 0% for those obtained from the crown of the mature tree 2 months after commencement of spring growth flush. Intermediate rootability was found for cuttings taken from 1-month-old seedlings and from the 2 week-old growth of suckers at the base of the mature tree. Rooting of cuttings with intermediate rooting potential was increased by a quick dip treatment with 2500 ppm indole-3-butyric acid (IBA) or by using apical rather than basal stem segments. Commercially acceptable rooting was not achieved using cuttings from root suckers or the crown of Aesculus ‘Augumn Splendor’.

scholarly journals Rooting of Vaccinium corymbosum L. microshoots cv. «Blue-Berry» in culture in vitro and ex vitro

2018 ◽  
Vol 7 (4) ◽  
pp. 80-84
Author(s):  
Gamil El-Dis Mohamed ◽  
Landysh Zavdetovna Khusnetdinova ◽  
Olga Arnoldovna Timofeeva
Keyword(s):  
Survival Rate ◽  
Root System ◽  
Butyric Acid ◽  
Activated Charcoal ◽  
Vaccinium Corymbosum ◽  
Ex Vitro ◽  
Axillary Shoots ◽  
Maximum Increase ◽  
Regenerated Plantlets

This paper discusses a method of micropropagation of Vaccinium corymbosum L. cv. Blue-Berry. The results showed that WPM supplemented with 1,0 mg/l of zeatin in combination with 0,1 mg/l of indolyl-3-butyric acid was more effective for the multiplication of blueberry axillary shoots. The maximum increase in the number of healthy axillary shoots was observed in the fourth subculture, whereas the phenomenon of hyperhydration (vitrification) began to appear in the fifth subculture. In addition, it was established that the presence of indolyl-3-butyric acid and 1,0 g/l of activated charcoal in the nutrient medium lead to the development of good root system of the Vaccinium corymbosum cv. Blue-Berry. The highest mean number of roots formed per explant was obtained on WPM medium, supplemented with 0,5 mg/l indolyl-3-butyric acid after 10 weeks. Acclimatization of in vitro regenerated plantlets of Vaccinium corymbosum with a developed root system in ex-vitro conditions (pH 3,5-4) showed a 100% survival rate.

scholarly journals POSTHARVEST CaCl2 DIPS INCREASE HIGHBUSH BLUEBERRY FIRMNESS

HortScience ◽  
1992 ◽  
Vol 27 (6) ◽  
pp. 582a-582
Author(s):  
Jane Beggs
Keyword(s):  
Vaccinium Corymbosum ◽  
Highbush Blueberry ◽  
Hard Surface ◽  
Immature Fruit ◽  
Pvc Pipe ◽  
Degree Of Damage ◽  
Highbush Blueberries ◽  
Water Tanks ◽  
Tank Water

Deformed or damaged berries reduce the grade of frozen highbush blueberries (Vaccinium corymbosum L.). Before berries are frozen, immature fruit are commonly removed by density sorting in water tanks. Three studies were conducted to determine if the firmness or quality of highbush blueberries could be improved by the addition of CaCl2 to sorting tank water. `Bluecrop' and `Jersey' berries were dipped in CaCl2 solutions (0.0-4.0%) for periods of 0.5-8.0 minutes. The effect of rinsing the fruit after treatment was also studied. Berries were held at 2C for several days before evaluation. A 61.5 cm length of PVC pipe (4.0 cm. I.D.) was filled to a depth of 50 cm. with berries and dropped 4 times on to a hard surface from a height of 10 cm. The compression of the column of berries was measured and berries were removed and visually sorted according to the degree of damage. The amount of compression and number of damaged berries were inversely related to the CaCl2 concentration. Rinsing berries immediately after dips negated the effects of CaCl2. Treatment with CaCl2 may result in objectionable flavors.

scholarly journals Concentrations of cytokinin and the use of agricultural residues in the in vitro propagation media of highbush blueberry

2020 ◽  
Vol 50 (6) ◽  
Author(s):  
Michele Carla Nadal ◽  
Adriane Marinho de Assis ◽  
Dianini Brum Frölech ◽  
Bruna Andressa dos Santos Oliveira ◽  
Cari Rejane Fiss Timm ◽  
...  
Keyword(s):  
Rice Husk ◽  
In Vitro Propagation ◽  
Culture Medium ◽  
Propagation Rate ◽  
Dry Mass ◽  
Vaccinium Corymbosum ◽  
Agricultural Residues ◽  
Highbush Blueberry ◽  
Rice Husks

ABSTRACT: The objective of the research was to evaluate cytokinin concentrations and agricultural residues as physical structuring agents of the culture medium in the in vitro propagation of highbush blueberry (Vaccinium corymbosum ‘Duke’). To that end, three different concentrations of 2-isopentenyladenine (2iP) (0, 5, and 10 mg L-1)in woody plant medium (WPM) and five structuring agents (in natura rice husks, carbonized rice husks, coconut fiber, S-10 Beifort®, and agar)were tested. After 60days of culture, the following parameters were evaluated: survival (%),sprout and shoot lengths (cm),propagation rate, number of sprouts, and shoot dry mass (g). The substrates used as structuring agents were analyzed in terms of pH and electrical conductivity. The in natura rice husk, S-10 Beifort®, and carbonized rice husk did not differ from the agar in terms of the sprout length. The shoots were longer (1.64 cm) in cultures within natura rice husk than in other treatments. In the absence of 2iP, S-10 Beifort® resulted in the highest propagation rate (2.97). Concerning the number of sprouts, S-10 Beifort®, in natura rice husks, and carbonized rice husks did not differ from agar. However, when the regulator was added, the agar performed better in comparison to the other residues. Thus, in natura rice husks, carbonized rice husks, and S-10 Beifort®are potential physical structuring agents of the culture medium that can be used without 2iP.

scholarly journals Determining Diuron, Simazine, and Methiocarb Residues in Highbush Blueberries (Vaccinium corymbosum)

HortScience ◽  
1993 ◽  
Vol 28 (1) ◽  
pp. 33-35 ◽  
Author(s):  
C.B. Ely ◽  
R.E. Frans ◽  
T.L. Lavy ◽  
R.E. Talbert ◽  
J.D. Mattice
Keyword(s):  
High Performance ◽  
Vaccinium Corymbosum ◽  
Highbush Blueberry ◽  
Reverse Phase Hplc ◽  
Reverse Phase ◽  
Tolerance Level ◽  
Ultraviolet Absorbance ◽  
Absorbance Detection ◽  
Highbush Blueberries ◽  
Over The Top

Two-year-old highbush blueberry bushes (Vaccinium corymbosum L. `Collins') were treated in Mar. 1985 with diuron or simazine at 2.2 or 4.5 kg a.i./ha. No residues were detected by reverse-phase high-performance liquid chromatography-ultraviolet absorbance detection (HPLC-UV) from treated berries that were harvested in June. Methiocarb was applied in May 1986 at 0.84 and 3 kg·ha-1 over the top of 3-year-old `Collins' when the berries began to ripen. Reverse-phase HPLC-UV of berries treated with methiocarb at 3 kg·ha-1 had combined residues of methiocarb and its sulfone and sulfoxide metabolites of 13.1 ppm from unrinsed and 7 ppm from rinsed berries harvested on the day of treatment; 4.9 ppm from unrinsed and 4 ppm from rinsed berries harvested 4 days after treatment; and 2.4 ppm from unrinsed and 2.5 ppm from rinsed berries harvested 8 days after treatment. Unrinsed berries treated with methiocarb at 0.84 kg·ha-1 had 5.7 ppm residue on the day of treatment and 1 ppm 8 days later. Residues from berries treated with methiocarb at 0.84 or 3 kg·ha-1 were below the legal tolerance level of 5 ppm after the required 7-day waiting period. Chemical names used: n'-(3,4-dichlorophenyl)-N,N -dimethylurea (diuron); 6-chloro- N,N' -diethyl-1,3,5-triazine-2,4-diamine (simazine); 3,5-dimethyl-4-(methylthio)phenol methylcarbamate (methiocarb).

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