A rapid method for detecting cold injury in conifer seedling root systems
A method is described for rapidly determining the extent of cold injury to Douglas-fir (Pseudotsugamenziesii (Mirb.) Franco) seedling root systems. It is based upon pressure:volume theory but short-cuts the tedious pressure:volume method. Injured root systems were brought to full hydration, then weighed and placed into a pressure chamber. A pressure of 1.5 MPa was applied for a 5-min period, then the root system was removed from the chamber and reweighed. The percent weight loss was then calculated. Percent weight loss was closely related to degree of low temperature exposure in Douglas-fir seedling roots. Percent weight loss values were near 5 in uninjured controls, whereas freeze-injured seedlings had percent weight loss values of 7 or greater. Percent weight loss values measured on seedlings just before planting were closely correlated with seedling vigor and mortality 3 months following planting in both a field test and a greenhouse pot trial.