Genetic diversity in a remnant population of lake trout (Salvelinus namaycush)

2009 ◽  
Vol 87 (7) ◽  
pp. 642-647 ◽  
Author(s):  
Tina M.J. Giroux ◽  
Douglas P. Chivers ◽  
Michael J. Fitzsimmons ◽  
Neil B. Chilton
Keyword(s):  
Genetic Diversity ◽  
National Park ◽  
Sequence Variation ◽  
Lake Trout ◽  
Single Strand Conformation Polymorphism ◽  
Salvelinus Namaycush ◽  
Nucleotide Variation ◽  
Prince Albert National Park ◽  
Conformation Polymorphism ◽  
Remnant Population

Genetic diversity of the remnant population of lake trout ( Salvelinus namaycush (Walbaum in Artedi, 1792)) in Crean Lake was compared with that for two other populations in the Prince Albert National Park (PANP) using single-strand conformation polymorphism (SSCP) and DNA sequencing analyses of two mtDNA genes. Although there was no sequence variation among individuals for nad5, six different nad2 haplotypes were detected. The Crean Lake population had the least number of haplotypes and lowest nucleotide variation. Also, one common nad2 haplotype in Crean Lake was not detected in the other populations. The results suggest that introductions of lake trout from Wassegam Lake into Crean Lake were unsuccessful with respect to the establishment and (or) reproduction of the stocked fish. Conservation of the remnant population of lake trout in Crean Lake is important because it is genetically distinct from other populations within PANP and in adjacent areas.

Could ecological release buffer suppression efforts for non-native lake trout (Salvelinus namaycush) in Yellowstone Lake, Yellowstone National Park?

2020 ◽  
Vol 77 (6) ◽  
pp. 1010-1025 ◽  
Author(s):  
John M. Syslo ◽  
Travis O. Brenden ◽  
Christopher S. Guy ◽  
Todd M. Koel ◽  
Patricia E. Bigelow ◽  
...  
Keyword(s):  
Yellowstone National Park ◽  
National Park ◽  
Lake Trout ◽  
Survival Rates ◽  
Salvelinus Namaycush ◽  
Native Range ◽  
Yellowstone Lake ◽  
Ecological Release ◽  
Demographic Rates ◽  
Stock Recruitment

Yellowstone Lake in Yellowstone National Park, USA, has the longest ongoing suppression program for non-native lake trout (Salvelinus namaycush) in the western USA. Harvest data from the suppression program, along with data from an assessment program initiated in 2011, was used to estimate lake trout abundance and mortality rates. Abundance and biomass estimates were used to estimate stock–recruitment dynamics, which were inputs to a simulation model forecasting responses to continued suppression. Abundance increased during 1998–2012 when total annual mortality exceeded 0.59 and declined thereafter. The fishing mortality rate required to reduce abundance was 67% greater than predicted by models that used prerecruit survival estimates from the lake trout’s native range. Prerecruit survival in Yellowstone Lake was estimated at four to six times greater than native range survival rates. Simulated abundance continued to decline if recent suppression efforts were maintained. High prerecruit survival in Yellowstone Lake likely illustrates ecological release for an invasive species in an ecosystem containing few predators or competitors and demonstrates the potential pitfalls of assuming equal demographic rates for native and non-native populations.

scholarly journals Detection of Genetic Diversity in Four Candidate Genes by Polymerase Chain Reaction-Single Strand Conformation Polymorphism (PCR-SSCP) Analysis of Goats in Sri Lanka

2016 ◽  
Vol 4 (1) ◽  
pp. 133-138
Author(s):  
H.B.P.C. Ariyarathne ◽  
LGS Lokugalappatti ◽  
H.B.S. Ariyaratne ◽  
D.M.S. Munasinghe
Keyword(s):  
Genetic Diversity ◽  
Polymerase Chain Reaction ◽  
Candidate Genes ◽  
Single Strand Conformation Polymorphism ◽  
Single Strand ◽  
Sri Lankan ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Phenotypic Groups ◽  
Conformation Polymorphism

Genetic diversity of Sri Lankan goats (Capra hircus) was evaluated using Polymerase Chain Reaction-Single Strand Conformation Polymorphism (PCR-SSCP) method to detect polymophism in four candidate genes (LALBA, k-CSN3, GnRHR and BMP4) in a sample of 219 goats representing three phenotypic groups. All studied loci were polymorphic, having three morphs for BMP4, LALBA and two morphs for k-CSN3 and GnRHR in the study population. A significant difference between genotypic frequencies for BMP4 and GnRHR genes was found while PIC criterion revealed an intermediate polymorphism for all analyzed phenotypic groups except for k-CSN3 and GnRHR genes. Cross-bred animals for BMP4 gene and nondescript animals for LALBA gene were comparatively more polymorphic according to the effective allele number criterion. This study provides insight into the genetic diversity of Sri Lankan goats, which can be utilized to develop single nucleotide polymorphism markers to be used in association studies, and marker assisted selection.Int J Appl Sci Biotechnol, Vol 4(1): 133-138

Response of non-native lake trout (Salvelinus namaycush) to 15 years of harvest in Yellowstone Lake, Yellowstone National Park

2011 ◽  
Vol 68 (12) ◽  
pp. 2132-2145 ◽  
Author(s):  
John M. Syslo ◽  
Christopher S. Guy ◽  
Patricia E. Bigelow ◽  
Philip D. Doepke ◽  
Brian D. Ertel ◽  
...  
Keyword(s):  
Growth Rate ◽  
Population Growth ◽  
Yellowstone National Park ◽  
National Park ◽  
Population Growth Rate ◽  
Lake Trout ◽  
Cutthroat Trout ◽  
Salvelinus Namaycush ◽  
Yellowstone Lake ◽  
Yellowstone Cutthroat Trout

Introduced lake trout ( Salvelinus namaycush ) threaten to extirpate native Yellowstone cutthroat trout ( Oncorhynchus clarkii bouvieri ) in the 34 000 ha Yellowstone Lake in Yellowstone National Park, USA. Suppression (and eventual eradication) of the lake trout population is deemed necessary for the conservation of Yellowstone cutthroat trout. A US National Park Service gill-netting program removed nearly 450 000 lake trout from Yellowstone Lake from 1995 through 2009. We examined temporal variation in individual growth, body condition, length and age at maturity, fecundity, mortality, and population models to assess the efficacy of the lake trout suppression program. Population metrics did not indicate overharvest despite more than a decade of fish removal. The current rate of population growth is positive; however, it is lower than it would be in the absence of lake trout suppression. Fishing effort needs to increase above observed levels to reduce population growth rate below replacement. Additionally, high sensitivity of population growth rate to reproductive vital rates indicates that increasing fishing mortality for sexually mature lake trout may increase the effectiveness of suppression. Lake trout suppression in Yellowstone Lake illustrates the complexities of trying to remove an apex predator to restore a relatively large remote lentic ecosystem with a simple fish assemblage.

Analysis of genetic diversity inCitrus

2011 ◽  
Vol 9 (2) ◽  
pp. 218-221 ◽  
Author(s):  
François Luro ◽  
Julia Gatto ◽  
Gilles Costantino ◽  
Olivier Pailly
Keyword(s):  
Genetic Diversity ◽  
Organic Acids ◽  
Metabolic Pathways ◽  
Single Strand Conformation Polymorphism ◽  
Strongly Correlated ◽  
Cultivation Conditions ◽  
Citrus Pulp ◽  
Genes Encoding ◽  
Citrus Juices ◽  
Conformation Polymorphism

Sugar and acidity levels are the main criteria of general fruit quality and for citrus juices pulp, in particular. The constituents of the acidity (organic acids) and the sweetness (glucose, fructose and sucrose) and the genes involved in their regulation have seldom been used to exploreCitrusgenetic diversity. We evaluated the juice composition of primary metabolic components for 87 varieties belonging to the eight majorCitrusspecies grown under the same environmental and cultivation conditions by HPLC. We investigated the sequence polymorphism of nine candidate genes encoding for key enzymes of sugars and organic acids metabolic pathways by single strand conformation polymorphism (SSCP). Whatever the biochemical or molecular analyses, the observed structure ofCitrusdiversity was organized around three groups corresponding to the ancestral species (mandarin, pummelo and citron). As expected, the secondary species were closely related to their putative ancestors except forCitrus aurantium. Biochemical diversity was strongly correlated to molecular SSCP diversity at the genus level but not at the intraspecific level. Compared with other molecular marker types, higher diversity has been observed with SSCP technology, which makes it suitable for future quantitative trait loci mapping approach on gene polymorphism in citrus pulp acidity and sweetness regulation.

scholarly journals Single-Strand Conformation Polymorphism Fingerprint Method for Dictyostelids

2021 ◽  
Vol 12 ◽  
Author(s):  
Phongthana Pasookhush ◽  
Asmatullah Usmani ◽  
Kowit Suwannahong ◽  
Prasit Palittapongarnpim ◽  
Kamolchanok Rukseree ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Large Scale ◽  
Single Strand Conformation Polymorphism ◽  
Ssu Rdna ◽  
Single Strand ◽  
Biological Research ◽  
Gene Sequences ◽  
Genetic Diversity Study ◽  
Conformation Polymorphism ◽  
Diversity Study

Dictyostelid social amoebae are a highly diverse group of eukaryotic soil microbes that are valuable resources for biological research. Genetic diversity study of these organisms solely relies on molecular phylogenetics of the SSU rDNA gene, which is not ideal for large-scale genetic diversity study. Here, we designed a set of PCR–single-strand conformation polymorphism (SSCP) primers and optimized the SSCP fingerprint method for the screening of dictyostelids. The optimized SSCP condition required gel purification of the SSCP amplicons followed by electrophoresis using a 9% polyacrylamide gel under 4°C. We also tested the optimized SSCP procedure with 73 Thai isolates of dictyostelid that had the SSU rDNA gene sequences published. The SSCP fingerprint patterns were related to the genus-level taxonomy of dictyostelids, but the fingerprint dendrogram did not reflect the deep phylogeny. This method is rapid, cost-effective, and suitable for large-scale sample screening as compared with the phylogenetic analysis of the SSU rDNA gene sequences.

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