Erythropoiesis in peripheral blood of tuatara (Sphenodon punctatus) and turtle (Malaclemys terrapin)

1970 ◽  
Vol 48 (2) ◽  
pp. 209-212 ◽  
Author(s):  
Vibeke E. Engelbert ◽  
Ann Dorothy Young
Keyword(s):  
Cell Population ◽  
Peripheral Blood ◽  
Total Cell ◽  
Red Cells ◽  
Malaclemys Terrapin ◽  
Domestic Birds ◽  
Total Cell Population ◽  
Sphenodon Punctatus ◽  
Clone Cells

Erythropoiesis in peripheral blood of domestic birds was shown earlier to arise directly from the nucleus of mature erythrocytes as nuclear protuberations that later broke free. Three to seven percent of new red cells originated in this way from clone cells.Investigations of peripheral blood in reptiles has further demonstrated formation of nucleated red cells as clones from nuclear buds of mature erythrocytes. Clone cells plus new immature red cells constitute, in Sphenodon punctatus, 8–18% and, in turtle, Malaclemys terrapin, 18–25% of the total cell population. Some lymphocytes and granulocytes appear also to arise from nuclear buds. Thrombocytes were present in some animals, absent in others, but were not counted in the present work.

scholarly journals Total Cell Counts of the Bone Marrow of Normal Albino Rats from 1 to 50 Weeks of Age

Blood ◽  
1959 ◽  
Vol 14 (4) ◽  
pp. 409-414 ◽  
Author(s):  
WILLIAM T. BURKE ◽  
CHARLES HARRIS
Keyword(s):  
Bone Marrow ◽  
Cell Population ◽  
Normal Values ◽  
Age Groups ◽  
Considerable Change ◽  
Total Cell ◽  
Albino Rats ◽  
Cell Counts ◽  
Total Cell Population ◽  
Femoral Bone Marrow

Abstract A method is described by which the total nucleated cell count of femoral bone marrow of the rat can be estimated and cell population expressed in terms of differential counts. Normal values of total nucleated cell counts and the cellular distributions are given for seven age groups. These data indicate considerable change in bone marrow total cell population in rats one to 10 weeks of age.

SPHEROPLAST INDUCTION AND LYSIS OF BCG STRAINS BY GLYCINE AND LYSOZYME

1966 ◽  
Vol 12 (2) ◽  
pp. 255-261 ◽  
Author(s):  
H. Sato ◽  
B. B. Diena ◽  
L. Greenberg
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Cell Population ◽  
Lithium Chloride ◽  
Synthetic Medium ◽  
Total Cell ◽  
Cell Populations ◽  
Standard Curve ◽  
Total Cell Population

Spheroplast induction and lysis of 6 BCG strains of Mycobacterium tuberculosis by glycine and lysozyme was studied in various media. Spheroplast production was noted in only three strains involving 20% of cell populations. Lysis, as distinct from spheroplast induction, occurred in Dubos medium containing 1.5% glycine and 0.01% lysozyme after 24–48 hours of incubation. Estimation from a standard curve indicated 40 to 70% lysis of the total cell population after 7–10 days of incubation.Similarly, lysis of BCG cells occurred when the inducers, glycine, lysozyme, and lithium chloride, were added to nitrogen-starved cultures grown in Aldridge synthetic medium for 7 to 8 days.

scholarly journals TOTAL CELL NUMBER IN FETAL BRAIN

2011 ◽  
Vol 19 (1) ◽  
pp. 35 ◽  
Author(s):  
Grethe Badsberg Samuelsen ◽  
Nenad Bogdanović ◽  
Henning Laursen ◽  
Niels Graem ◽  
Jørgen Falck Larsen ◽  
...  
Keyword(s):  
Birth Weight ◽  
Cell Population ◽  
Fetal Brain ◽  
Fold Increase ◽  
Cell Number ◽  
Total Cell ◽  
Cortical Plate ◽  
Total Cell Number ◽  
Normal Birth ◽  
Total Cell Population

In this study the material comprises brains from three aborted fetuses and two fullterm infants who died at birth.The gestational ages ranged from the 22nd week to term. All cases were without malformations, known chromosomal abnormality, hydrops, and systemic infections, and all had normal birth weights with fetal growth indices (observed birth weight/expected mean birth weight) between 0.9 - 1.05. The preliminary results show a five fold increase in the total cell population in the marginal zone/cortical plate, MZ/CP (future neocortex), from week 22 until term. In the transient subplate zone, SP, the total cell number was more than doubled from week 22 to week 30-35, and then decreased towards term. In the intermediate zone, IZ (future white matter), the total cell population was doubled from week 22 until term. The total cell number in the entricular/subventricular zone, VZ/SZ (germinal matrix), was reduced by a factor of five from week 22 until term. A histological differentiation between neurons and glial cells was not possible. The optical fractionator was used to estimate the total cell population in four characteristic developmental zones in the human fetal brain. Fetal brain tissue undergoes considerable and rather unpredictable shrinkage during fixation. However, using the fractionator principle it is possible to eliminate this problem, provided that the structure of interest (one brain hemisphere) is fully intact.

Pattern formation in Dictyostelium discoideum

Development ◽  
1977 ◽  
Vol 40 (1) ◽  
pp. 215-228
Author(s):  
D. Forman ◽  
D. R. Garrod
Keyword(s):  
Life Cycle ◽  
Pattern Formation ◽  
Dictyostelium Discoideum ◽  
Cell Population ◽  
Growth Conditions ◽  
Total Cell ◽  
Immunofluorescent Staining ◽  
Fruiting Bodies ◽  
Fluorescent Intensity ◽  
Total Cell Population

Immunofluorescent staining of the prespore cells of the cellular slime mould Dictyostelium discoideum was carried out using a heterologous spore antibody. The highly specific staining of the prespore vesicles (PSVs) within the prespore cells enabled quantitative determinations to be made of the rate and extent of development of these cells throughout the life cycle. The results showed that PSVs first appeared in a large proportion of the cells shortly after the cells had chemotactically aggregated into multicellular masses. During the later phases of the life cycle, the proportion of cells containing PSV increased, as did the fluorescent intensity of their PSVs, until the early culmination stage of development when 85–90 % of the total cell population contained PSVs. Lowering the temperature of development delayed the onset of vesicle formation and decreased the proportion of prespore cells in the total cell population. Changing the growth conditions of the cells prior to multicellular development also had a significant effect on the proportions of prespore cells, as did the use of a mutant known to give rise to fruiting bodies with a reduced number of spores. The comparability between these estimates of prespore cell proportions at culmination and previously reported spore:stalk ratios within fruiting bodies confirms the view that PSVs are reliable indicators of prespore cells. The finding that temperature and growth conditions and the use of mutants all of which are known to affect spore:stalk ratios, also all affected prespore proportions in the expected direction, adds further weight to this argument. The fact that prespore cells are beginning to differentiate early in the multicellular phase of the life cycle and the related finding that such differentiation always precedes formation of the grex tip are results of considerable importance to the development of a model for pattern formation in D. discoideum.

EFFECT OF THE CESSATION OF THIOURACIL ADMINISTRATION ON THE CELL POPULATION OF THE THYROID OF RATS

1957 ◽  
Vol 16 (1) ◽  
pp. 1-8 ◽  
Author(s):  
JOYCE E. SANTLER
Keyword(s):  
Thyroid Gland ◽  
Blood Vessel ◽  
Cell Population ◽  
Stromal Cells ◽  
Stromal Cell ◽  
Normal Values ◽  
Total Cell ◽  
Albino Rats ◽  
Follicular Cells ◽  
Total Cell Population

SUMMARY The thyroid gland of adult male albino rats enlarged during a period of 30 days of thiouracil treatment. During 75 days after cessation of this treatment there was a regression of the enlarged gland, but not to normal values of weight, volume or total cell population. The regression did not, however, involve any appreciable drop in the numbers of follicular cells. Decrease in the volume of follicular tissue was therefore due to shrinkage of individual follicular cells. The stromal cell population decreased, especially during the first 7 days after the withdrawal of thiouracil, and at the end of the experiment the proportion of stromal cells to follicular cells had returned to normal. The first 7 days was also the time of greatest regression in weight, volume and blood vessel space in the thyroid, these values showing only slight further reduction in the groups killed 30 and 75 days after the withdrawal of goitrogen.

Cellular proliferation in complicated versus uncomplicated atherosclerotic lesions: Total cell population, foam cells and newly formed microvessels

2009 ◽  
Vol 41 (6) ◽  
pp. 408-413 ◽  
Author(s):  
P. Manolakou ◽  
R. Angelopoulou ◽  
C. Bakoyiannis ◽  
E. Psathas ◽  
E. Bastounis ◽  
...  
Keyword(s):  
Cell Population ◽  
Cellular Proliferation ◽  
Foam Cells ◽  
Total Cell ◽  
Atherosclerotic Lesions ◽  
Total Cell Population

scholarly journals Mathematical models incorporating a multi-stage cell cycle replicate normally-hidden inherent synchronization in cell proliferation

2019 ◽  
Vol 16 (157) ◽  
pp. 20190382 ◽  
Author(s):  
Sean T. Vittadello ◽  
Scott W. McCue ◽  
Gency Gunasingh ◽  
Nikolas K. Haass ◽  
Matthew J. Simpson
Keyword(s):  
Experimental Data ◽  
Cell Cycle ◽  
Cell Proliferation ◽  
Cell Population ◽  
Total Cell ◽  
Fluorescent Cell ◽  
Multi Stage ◽  
Total Cell Population ◽  
Cycle Dependent ◽  
Proliferation Assays

We present a suite of experimental data showing that cell proliferation assays, prepared using standard methods thought to produce asynchronous cell populations, persistently exhibit inherent synchronization. Our experiments use fluorescent cell cycle indicators to reveal the normally hidden cell synchronization, by highlighting oscillatory subpopulations within the total cell population. These oscillatory subpopulations would never be observed without these cell cycle indicators. On the other hand, our experimental data show that the total cell population appears to grow exponentially, as in an asynchronous population. We reconcile these seemingly inconsistent observations by employing a multi-stage mathematical model of cell proliferation that can replicate the oscillatory subpopulations. Our study has important implications for understanding and improving experimental reproducibility. In particular, inherent synchronization may affect the experimental reproducibility of studies aiming to investigate cell cycle-dependent mechanisms, including changes in migration and drug response.

Direct measurement of microvessel hematocrit, red cell flux, velocity, and transit time

1982 ◽  
Vol 243 (6) ◽  
pp. H1018-H1026 ◽  
Author(s):  
I. H. Sarelius ◽  
B. R. Duling
Keyword(s):  
Direct Measurement ◽  
Cell Population ◽  
Transit Time ◽  
Volume Fraction ◽  
Total Cell ◽  
Cheek Pouch ◽  
Red Cell ◽  
Hamster Cheek Pouch ◽  
Total Cell Population

A method is presented for the in vivo study of red cell flow dynamics. The method permits direct measurement of the red cell volume fraction in microvessel blood without resort to in vitro calibration curves. Furthermore, the method does not require extensive mathematical manipulation and can be applied to any microvascular network in any tissue. The method also enables direct measurement of red cell velocity, flux, and capillary transit time. Fluorescently labeled erythrocytes in tracer quantities, but known concentrations, are used as indicators of the behavior of the total cell population. Erythrocyte transit time across vascular networks and erythrocyte velocity are determined directly by following the behavior of the labeled cells. Hematocrit and red cell flux are measured by standard microcirculatory methods using labeled cells instead of the total cell population. Data are then converted to absolute values from the measured fraction of labeled cells. The method is thus absolutely dependent on the labeled cells being rheologically normal, and the conditions under which this requirement is satisfied are defined. Microvascular data obtained by the use of this method are presented for hamster cheek pouch and cremaster muscle.

scholarly journals Mathematical models incorporating a multi-stage cell cycle replicate normally-hidden inherent synchronisation in cell proliferation

2019 ◽  
Author(s):  
Sean T. Vittadello ◽  
Scott W. McCue ◽  
Gency Gunasingh ◽  
Nikolas K. Haass ◽  
Matthew J. Simpson
Keyword(s):  
Experimental Data ◽  
Cell Cycle ◽  
Cell Proliferation ◽  
Cell Population ◽  
Total Cell ◽  
Fluorescent Cell ◽  
Multi Stage ◽  
Total Cell Population ◽  
Cycle Dependent ◽  
Proliferation Assays

AbstractWe present a suite of experimental data showing that cell proliferation assays, prepared using standard methods thought to produce asynchronous cell populations, persistently exhibit inherent synchronisation. Our experiments use fluorescent cell cycle indicators to reveal the normally-hidden cell synchronisation by highlighting oscillatory subpopulations within the total cell population. These oscillatory subpopulations would never be observed without these cell cycle indicators. On the other hand, our experimental data show that the total cell population appears to grow exponentially, as in an asynchronous population. We reconcile these seemingly inconsistent observations by employing a multi-stage mathematical model of cell proliferation that can replicate the oscillatory subpopulations. Our study has important implications for understanding and improving experimental reproducibility. In particular, inherent synchronisation may affect the experimental reproducibility of studies aiming to investigate cell cycle-dependent mechanisms, including changes in migration and drug response.

Parallel changes between the percentage of fetal pituitary cells immunoreactive to oestrogen receptor α and the concentration of 17β-oestradiol in fetal and maternal plasma during gestation in sheep

2004 ◽  
Vol 16 (6) ◽  
pp. 611 ◽  
Author(s):  
Sheng Cui ◽  
Jia li Liu ◽  
Yu jing Shao ◽  
Jian Chao Zhang
Keyword(s):  
Pituitary Gland ◽  
Cell Population ◽  
Oestrogen Receptor ◽  
Maternal Plasma ◽  
Total Cell ◽  
Pituitary Cells ◽  
Fetal Sheep ◽  
Fetal Plasma ◽  
Total Cell Population ◽  
The Relationship

The present study was designed to investigate the relationship between fetal sheep pituitary oestrogen receptor (ER) α expression and changes in fetal and maternal plasma 17β-oestradiol (E2) concentrations during gestation. The results revealed that immunoreactivity for ERα was located in the nuclei and distributed throughout the fetal pituitary gland during gestation. The percentage of ERα-positive cells was approximately 2% of the total cell population in female fetuses at Day 60 of gestation, increased to approximately 7% and 13% of the total cell population at Days 90 and 120 of gestation, respectively, and then declined to approximately 10% at birth. The fetal plasma E2 concentrations were approximately 19 and 71 pg mL−1 at Days 90 and 120 of gestation, respectively, and decreased to 22 pg mL−1 after birth. In male fetuses, plasma E2 concentrations and the percentage of ERα-positive cells were similar to values in female fetuses throughout gestation, except on Day 120 when the plasma E2 level in female fetuses was significantly higher than in male fetuses. These data demonstrate that changes in the percentage of fetal pituitary ERα-positive cells parallel fetal plasma E2 concentrations throughout gestation.

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