Seasonal histochemistry of the renal sexual segment in male common water snakes, Nerodia sipedon (L.)

1984 ◽  
Vol 62 (9) ◽  
pp. 1737-1740 ◽  
Author(s):  
Michael R. Weil
Keyword(s):  
Secretory Granules ◽  
Periodic Acid ◽  
Early Spring ◽  
Female Reproductive Tract ◽  
Bromphenol Blue ◽  
Nerodia Sipedon ◽  
Periodic Acid Schiff ◽  
Active Season ◽  
Late Autumn ◽  
Water Snakes

The histochemistry of the renal sexual segment of male common water snakes, Nerodia sipedon (L.), was studied throughout the active season. This segment is hypertrophied in early spring and late autumn, coinciding with peaks in plasma androgen levels. Secretory granules, present in epithelial cells lining the kidney tubule lumen of the sexual segment, are most evident at these times. Granules stain most intensely with periodic acid – Schiff, Sudan black B, and Oil red O from snakes collected in early spring and nearly as strongly from snakes collected in late autumn. Mercuric bromphenol blue and the Millon reaction stain granules most intensely from autumn captured animals. Granules from midsummer animals stained weakly with all of the above stains. It is suggested that the renal sex segment secretion plays a dual role, one of sperm transport and capacitation (final sperm maturation in the female reproductive tract) in autumn and another related to sexual behavior in the spring.

Pituitary Adenomas that Produce Adrenocorticotropic Hormone and A-Subunit: Clinicopathological, Immunohistochemical, Ultrastructural, and Immunoelectron Microscopic Studies in Nine Cases

Neurosurgery ◽  
1990 ◽  
Vol 26 (3) ◽  
pp. 397-403 ◽  
Author(s):  
Kathryn K. Berg ◽  
Bernd W. Scheithauer ◽  
Ignacio Felix ◽  
Kalman Kovacs ◽  
Eva Horvath ◽  
...  
Keyword(s):  
Pituitary Adenomas ◽  
Adrenocorticotropic Hormone ◽  
Secretory Granules ◽  
Preoperative Evaluation ◽  
Periodic Acid ◽  
Periodic Acid Schiff ◽  
Double Labeling ◽  
Corticotroph Adenoma ◽  
A Subunit ◽  
Corticotroph Adenomas

Abstract Eight surgical and one autopsy specimen of pituitary adenomas (six cases of Cushing's disease, two of Nelson's syndrome. and one of hypopituitarism) were studied by histochemical, immunohistocytological, and ultrastructural methods. Eight tumors showed the characteristic histochemical profile of corticotroph adenoma—amphophilic to basophilic, and periodic acid-Schiff-positive to some extent. In all tumors, immunohistochemical studies revealed adrenocorticotropic hormone (ACTH) and à-subunit in the cytoplasm of some adenoma cells. By electron microscopy, seven tumors were found to be monomorphous; six were typical corticotroph adenomas and one was a subtype II silent corticotroph adenoma. One unique lesion was bimorphous—i.e., composed of corticotrophs as well as cells resembling glycoprotein cells. Immunoelectron microscopy by the double-labeling immunogold technique, performed on one corticotroph adenoma, demonstrated the presence of ACTH and à-subunit not only within the same adenoma cells but also within the same secretory granules. The cytogenesis of ACTH à-subunit tumors, a rare form of plurihormonal adenoma. remains to be elucidated. The duration of disease associated with these tumors exceeded the duration in patients with ordinary corticotroph adenomas. Given the low frequency with which increases in serum à-subunit are detectable in patients with such tumors—13% in this series—hormone production is not recognized at preoperative evaluation.

Some Secretory Products of the Invasive Stage of a Digenetic Trematode

1968 ◽  
Vol 26 ◽  
pp. 156-157
Author(s):  
Paul L. Krupa ◽  
Arya K. Bal ◽  
Gilles H. Cousineau
Keyword(s):  
Secretory Granules ◽  
Periodic Acid ◽  
Cell Types ◽  
Digenetic Trematode ◽  
Width Ratio ◽  
Uniform Density ◽  
Acid Hydrolases ◽  
Periodic Acid Schiff ◽  
Gland Cells ◽  
Secretory Products

The fine structure of various gland cells and their secretory products was studied in the invasive stage (cercaria) of the platyhelminth parasite, Cryptocotyle lingua. Secretory granules or droplets occur in several different specialized cell types, but those that we call attention to here are found in the (1) surface cytoplasmic tegument or “cuticle”, (2) ducts of cephalic (penetration) glands, and (3) epithelial lining of the “excretory bladder”.The tegumental granules appear as numerous, membrane-bounded circular or oval profiles of uniform density (Fig. 1). They are scattered more or less randomly among mitochondria and other inclusion bodies of the tegument. Some of the longer granules, with a length to width ratio of about 7 to 1, have their long axes oriented perpendicularly to the surface plasma membrane of the parasite. In cercariae tested for acid hydrolases with sodium β-glycerophosphate in Barka and Anderson's modification of Gomori's medium, clumps of reaction product appear in the vicinity of the granules and elsewhere within the tegument, but not within the granules themselves. As granules that stain with periodic acid-Schiff, they are seen in certain subsurface gland cells as well as in the tegument under the light microscope.

scholarly journals Immunocytochemical localization of glycoprotein hormones in the rat anterior pituitary. A light and electron microscope study using antisera against rat bet subunits: a comparison between preembedding and postembeeding methods.

1980 ◽  
Vol 28 (2) ◽  
pp. 101-114 ◽  
Author(s):  
C Tougard ◽  
R Picart ◽  
A Tixier-Vidal
Keyword(s):  
Electron Microscope ◽  
Binding Sites ◽  
Secretory Granules ◽  
Periodic Acid ◽  
Light And Electron Microscopy ◽  
Thyroid Stimulating Hormone ◽  
Periodic Acid Schiff ◽  
Thyrotropic Cells ◽  
Rat Thyroid ◽  
Stimulating Hormone

The binding sites of antisera (anti) to the beta (beta) subunits of rat follicle-stimulating hormone (rFSH), rat luteinizing hormone (rLH), and rat thyroid-stimulating hormone (rTSH) have been localized in rat anterior pituitaries by immunocytochemistry using light and electron microscopy. With the light microscope, LHbeta and FSHbeta were found in the same cells, which were violet after the alcian blue-periodic acid Schiff (AB-PAS) staining. TSHbeta was found in polygonal or stellate cells that were blue after AB-PAS. With the electron microscope, the thyrotropic cells contained very small secretory granules. LHbeta and FSHbeta were found in various types of cells (types A and B and their intermediate forms), which had previously been identified as gonadotropic cells. On serial ultrathin sections using the postembedding method the same cells and even some granules inside these cells were stained by both anti-rLHbeta and anti-rFSHbeta. A comparison of binding sites of anti-rLHbeta was performed using the preembeeding and the postembeeding methods. Antigenicity was observed on secretory granules whatever the method used. However, binding sites of anti-rLHbeta were detected inside the cisternae of the rough endoplasmic reticulum only with the preembedding method.

Adenoid Cystic Carcinoma of the Bartholin Gland: An Overview

2007 ◽  
Vol 131 (5) ◽  
pp. 796-798 ◽  
Author(s):  
Francesca Maia Woida ◽  
Alfredo Ribeiro-Silva
Keyword(s):  
Adenoid Cystic Carcinoma ◽  
World Literature ◽  
Reproductive Tract ◽  
Periodic Acid ◽  
High Capacity ◽  
Lymphatic Invasion ◽  
Female Reproductive Tract ◽  
Periodic Acid Schiff ◽  
Adenoid Cystic ◽  
Regional Lymphadenectomy

Abstract Vulvar carcinoma is the fourth most frequently encountered malignancy of the female reproductive tract. Among vulvar neoplasms, 0.1% to 5% are of Bartholin gland origin. Primary adenoid cystic carcinoma of the Bartholin gland is very rare. To date, only about 60 cases have been reported in the world literature. Microscopic examination reveals a neoplasm of cribriform pattern composed of nests and columns of cells of bland appearance arranged concentrically around glandlike spaces filled with eosinophilic periodic acid-Schiff–positive diastase-resistant material. Immunohistochemically, the tumor cells express low-molecular-weight keratins, carcinoembryonic antigen, lysozyme, α1-antichymotrypsin, S100, and type IV collagen. Adenoid cystic carcinoma of the Bartholin gland is a slow-growing but locally very aggressive neoplasm with high capacity for recurrence. Perineural and lymphatic invasion is characteristic of this tumor and may explain its propensity for causing pain and recurrence. The treatment may range from simple local excision to radical vulvectomy, with or without partial to complete regional lymphadenectomy.

scholarly journals LIGHT AND ELECTRON MICROSCOPE LOCALIZATION OF BINDING SITES OF ANTIBODIES AGAINST OVINE LUTEINIZING HORMONE AND ITS TWO SUBUNITS IN RAT ADENOHYPOPHYSIS USING PEROXIDASE-LABELED ANTIBODY TECHNIQUE

1973 ◽  
Vol 58 (3) ◽  
pp. 503-521 ◽  
Author(s):  
C. Tougard ◽  
B. Kerdelhue ◽  
A. Tixier-Vidal ◽  
M. Jutisz
Keyword(s):  
Electron Microscope ◽  
Luteinizing Hormone ◽  
Binding Sites ◽  
Secretory Granules ◽  
Periodic Acid ◽  
Light And Electron Microscopy ◽  
Type A ◽  
Periodic Acid Schiff ◽  
Antibody Technique ◽  
A Cells

The binding sites of antisera generated in the guinea pig against ovine luteinizing hormone (oLH) and its two subunits (oLHα and oLHß) have been localized in rat anterior pituitaries taken from normal or castrated males and from ovariectomized females with the peroxidase-labeled antibody method, using light and electron microscopy. With the light microscope, the cells positive with antiserum to ovine luteinizing hormone (A-oLH) were violet after the Alcian blue-periodic acid-Schiff (AB-PAS) staining; they were also positive for A-oLHα and for A-oLHß and, from castrated males, they displayed an increased affinity for A-oLHß. Another cell type which was blue after the AB-PAS method reacted with the A-oLHα only; these cells, presumably thyrotropic cells, were retracted after castration and, besides their affinity for A-oLHα, acquired an affinity for A-oLHß. As seen through the electron microscope, two cell types were positive for A-oLH, A-oLHß, and A-oLHα and may be identified as luteinizing hormone-secreting cells. Type A cells were characterized by two classes of rounded, secretory granules. Type B cells were smaller and contained only small secretory granules. 1 mo after the rats were castrated the type A cells were hypertrophied and vacuolized. In both cases the secretory granules were the main sites of the antigenicity with the three antisera. A positive reaction was also found in the cytoplasm, particularly in hypertrophied cells from ovariectomized females and with A-oLHß. The cisternae of the rough endoplasmic reticulum were usually negative, except in highly degranulated cells from ovariectomized females and with A-oLHß.

Perineal swelling secondary to a vaginal wall cyst in a bitch in dioestrus

2019 ◽  
Vol 7 (3) ◽  
pp. e000805
Author(s):  
Cesar Sanchez Jimenez ◽  
Ana Rita Ramalho Furtado ◽  
Valéria Café Marçal ◽  
Darren Barnes ◽  
Emilie Fauchon
Keyword(s):  
Reproductive Tract ◽  
Periodic Acid ◽  
Vaginal Wall ◽  
Cystic Mass ◽  
Female Reproductive Tract ◽  
Intact Female ◽  
Periodic Acid Schiff ◽  
History Of ◽  
Perineal Swelling ◽  
Slow Growing

Vaginal cysts are a rare pathology of the female reproductive tract and are usually asymptomatic and often incidental in human and veterinary medicine. A seven-year and 11-month-old intact female bearded collie was referred for further investigations of a two-and-a-half-week history of a slow-growing perineal swelling that was noticed three to four weeks following oestrus. Ultrasound and CT images were acquired, which revealed that an intravaginal cystic mass was the cause of the aforementioned swelling. Following removal of the mass, histopathology revealed a fully excised vaginal cyst lined by simple cuboidal periodic acid-Schiff-positive epithelium most consistent with paramesonephric (Müllerian) origin. To the authors’ knowledge this is the first reported case of an extra-abdominal vaginal wall cyst in a bitch in dioestrus causing perineal swelling.

Exosomal secretion of death bullets: a new way of apoptotic escape?

2012 ◽  
Vol 303 (8) ◽  
pp. E1015-E1024 ◽  
Author(s):  
Nina Trokovic ◽  
Raimo Pöllänen ◽  
Pauliina Porola ◽  
Vasily Stegaev ◽  
Udo Hetzel ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Secretory Granules ◽  
Periodic Acid ◽  
Caspase 8 ◽  
Health State ◽  
Androgen Deficiency ◽  
Periodic Acid Schiff ◽  
Antiapoptotic Effect ◽  
Effector Caspase ◽  
Dhea Treatment

Ovariectomy/estrogen deficiency causes selective apoptosis of the serous epithelial cells of the submandibular glands (SMG) in female mice. Because such apoptosis does not occur in healthy, estrogen-deficient male mice, it was hypothesized that dihydrotestosterone (DHT) protects epithelial SMG cells against apoptosis. The antiapoptotic effect of DHT on human epithelial HSG cells exposed to tumor necrosis factor-α and cycloheximide was studied. Correspondingly, the proapoptotic effect of androgen deficiency was studied in orchiectomized (ORX) androgen-knockout (ARKO) and wild-type (WT) mice. The health state of the SMG cells was studied with Alcian blue-periodic acid Schiff (AB-PAS) and amylase staining and transmission electron microscopy (TEM). The eventual protective antiapoptotic effect of dehydroepiandrosterone (DHEA) treatment was tested in this model. Apoptosis was assessed using immunohistochemisty of cleaved effector caspase-3 and its activator caspase-8 and the TUNEL assay. To test for the bioavailability, intracrine metabolism and sex steroid effects of DHEA, cystein-rich secretory protein-3 (CRISP-3), and leucine-isoleucine-valine transport system 1 (LIV-1) were used as androgen- and estrogen-regulated biomarkers, respectively. DHT protected HSG cells against induced apoptosis. In mice, androgen deficiency resulted in extensive activation of apoptotic caspase-8/3 cascade in serous epithelial cells. However, in salivary glands, active caspases were not translocated to nuclei but secreted to salivary ducts in exosome-like particles, which are associated with weak AB-PAS and amylase staining of the androgen-deprived cells and reduced number of intracellular secretory granules. DHEA treatment suppressed induction of proapoptotic caspases and almost normalized mucins and amylase and ultramophology of the serous epithelial cells in WT ORX but not ARKO ORX mice. According to the CRISP-3 and LIV-1 markers, DHEA probably exerted its effects via intracrine conversion to DHT.

scholarly journals Adenocarcinoma of Apocrine Sweat Glands in a Mouflon (Ovis Musimon)

2005 ◽  
Vol 17 (4) ◽  
pp. 389-392 ◽  
Author(s):  
Federico Morandi ◽  
Cinzia Benazzi ◽  
Paolo Simoni
Keyword(s):  
Epithelial Cells ◽  
Lymph Nodes ◽  
Secretory Granules ◽  
Periodic Acid ◽  
Sweat Glands ◽  
Left Shoulder ◽  
Free Living ◽  
Periodic Acid Schiff ◽  
Ovis Musimon ◽  
Ultrastructural Findings

A free-living mouflon ( Ovis musimon) was presented with a mass on the left shoulder. At necropsy, multifocal, slightly protruding whitish spots were noted on the kidneys, and several lymph nodes were abnormal. Histologically, the mass was composed of epithelial cells arranged in tubular and tubulopapillary structures. The cytoplasm of the epithelial cells had numerous periodic acid–Schiff-positive and diastase-resistant granules. Ultrastructurally, the cytoplasm of the neoplastic cells contained numerous pleomorphic secretory granules and microvilli, which partially covered the luminal surface of the tumor cells. Metastatic foci were present in pres-capular and mediastinal lymph nodes and kidneys. On the basis of histological and ultrastructural findings, this tumor was diagnosed as a tubulopapillary adenocarcinoma, arising from apocrine sweat glands of the skin.

scholarly journals STRUCTURE OF SOME STRIGEATAS' ORGAN OF BRANDES

2019 ◽  
pp. 399-403
Author(s):  
Nacheva ◽  
Manikovskaya
Keyword(s):  
Periodic Acid ◽  
Secretory Activity ◽  
Bromphenol Blue ◽  
Main Function ◽  
Periodic Acid Schiff ◽  
Alaria Alata ◽  
Sudan Black B ◽  
Glandular Cells ◽  
Secondary Function ◽  
Schiff Reaction

The purpose of the research: the analysis of histology and histochemistry of the Brandes organ of Strigea strigis and Alaria alata. The trematode marites of Strigea striges and Alaria alata were the material. Fixation of the material was carried out in 10% neutral formalin. The treatment of the specimens was carried out using the conventional histological and histochemical methods: with hematoxylin-eosin according to the method of Van Gizon, Mallory, with Sudan black B, sulema-Bromphenol blue according to Bonhage, with periodic acid Schiff reaction by Mac-Manus, alcian blue according to Stedman and Mowry and with toluidine blue. Histochemical reactions were performed with appropriate controls. The studies have shown that the structure of the Brandes organ of Strigea strigis and Alaria alata differs by their constituents and morphology of glandular cells. The histochemical reactions are similar. The cells of the glandular complex show bromophenolophilia, toluudinophilia and fuchsinophilia in periodic acid Schiff reaction speaks about the glycoprotein nature of the secreted substances. Bromphenophilia and sudanophilia of glandular cells cytoplasm indicate the presence of lipoprotein substances in them. The Brandes of S. strigis and A. alata is a morphofunctional unit, to which the principle of multi-functionality is inherent. It performs the main function – digestion of food components by means of developed glandular structures and specialized secretory activity. Its ability to fix the helminth tightly in the endostatin can be considered a secondary function of the organ.

scholarly journals Peritoneal and scrotal carcinomas of unknown origin in two bovine calves

2018 ◽  
Vol 30 (4) ◽  
pp. 609-613 ◽  
Author(s):  
Kelsey A. Brakel ◽  
Grace VanHoy ◽  
Austin Hinds ◽  
Justin Breitbach ◽  
Christopher Premanandan ◽  
...  
Keyword(s):  
Medical Center ◽  
Secretory Granules ◽  
Periodic Acid ◽  
Unknown Origin ◽  
Abdominal Distension ◽  
Final Diagnosis ◽  
Anaplastic Carcinoma ◽  
Presumptive Diagnosis ◽  
Periodic Acid Schiff ◽  
The Ohio State University

Two unrelated bovine beef calves, aged 2 mo and 3 mo, were presented to The Ohio State University Veterinary Medical Center because of scrotal swelling and abdominal distension. On postmortem examination, there was abundant peritoneal fluid and numerous small friable masses covering all peritoneal surfaces and extending into the scrotum via the tunica vaginalis, with no identifiable primary neoplasm. Based on light microscopy, differential diagnoses included malignant mesothelioma and anaplastic carcinoma. Immunohistochemically, the neoplasms labeled positive for cytokeratin, and negative for vimentin and calretinin. Neoplastic cells contained periodic acid–Schiff-positive, diastase-resistant cytoplasmic granules, and lacked Alcian blue–positive, hyaluronidase-negative cytoplasmic vacuoles. Ultrastructurally, the cells had features of carcinoma, including secretory granules, and lacked typical features of mesothelioma, such as long slender microvilli. Our final diagnosis was carcinoma in both calves, despite the equivocal gross and light microscopic findings. We propose that a presumptive diagnosis of peritoneal mesothelioma in bovine calves should be avoided without corroboration by a combination of histology, histochemistry, immunohistochemistry, and, if possible, electron microscopy.

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