Phototaxis of adult brine shrimp, Artemia salina

1984 ◽  
Vol 62 (12) ◽  
pp. 2357-2359 ◽  
Author(s):  
David J. Bradley ◽  
Richard B. Forward Jr.

Using phototaxis as a behavioral measure of light perception, spectral and intensity sensitivity were determined for adult brine shrimp, Artemia salina. Spectral sensitivity was characterized by a well-defined maximum at 520 nm light, with perhaps a smaller maximum at 400 nm light. Dark-adapted animals showed a strong negative phototaxis at high intensities between 5.0 × 1012 quanta∙m−2∙s−1 and 3.6 × 1016 quanta∙m−2∙s−1 at 520 nm light. Positive phototaxis was greatest at the low intensity of 2.8 × 1012 quanta∙m−2∙s−1. Threshold sensitivity for both positive and negative phototaxis was approximately 2 × 1012 quanta∙m−2∙s−1.

1978 ◽  
Vol 56 (4) ◽  
pp. 708-711 ◽  
Author(s):  
Ronald B. Aiken ◽  
Jack P. Hailman

The brine shrimp Artemia salina was photopositive in response to monochromatic light of approximately equal quantum intensity of two absolute levels. At the higher level (about 3 × 1014 quanta s−1 cm−2), the spectral response was bimodal with peaks near 560 and 700 THz frequency (about 535 and 430 nm wavelength, respectively), with an antimode at about 640 THz (465 nm). Response at the lower level (about 2 × 1014) was slightly lower and the midspectrum peak was shifted to higher frequencies (shorter wavelengths) and depressed relative to the high-frequency peak near 700 THz. The results suggest that positive phototaxis is mediated by two visual pigments, the high-frequency (short-wavelength) pigment being activated at lower illuminations than the midspectrum pigment. A similar bimodal response is known from a crab larva, but this appears to be the first anostracan crustacean to have been tested.


1968 ◽  
Vol 48 (1) ◽  
pp. 39-53 ◽  
Author(s):  
J. H. S. BLAXTER

1. Herring larvae are characterized by eyes with no rods and no retinomotor responses, though both are present in the adult. 2. By observing the extinction of phototactic behaviour and feeding in herring larvae of different ages held at low intensities of white and coloured light, it was possible to obtain a measure of both threshold light intensities and spectral sensitivity for a pure-cone eye. 3. The phototaxis was positive at higher intensities, the threshold being 100 to 10-1 m.c. Below this threshold a negative phototaxis was observed which disappeared at a much lower threshold, about 10-5 to 10-6 m.c. The threshold for feeding varied with age and the type of food organism, lying between 10-1 and 10-2 m.c. 4. The high sensitivity for negative phototaxis was probably a visual response and not one mediated by a dermal light sense. 5. Spectral sensitivity varied depending on age and behavioural criteria. Using feeding and positive phototaxis the action spectrum was broad and plateau-like with a maximum in the yellow-green and would appear to be similar to other photopic curves. The action spectra for negative phototaxis showed a number of peaks. At hatching three peaks at 450, 520 and 600 mµ might represent three types of cone as postulated by the Young-Helmholtz theory of colour vision.


1974 ◽  
Vol 52 (3) ◽  
pp. 231-240 ◽  
Author(s):  
A. H. Warner ◽  
P. C. Beers ◽  
F. L. Huang

An enzyme that catalyzes the synthesis of P1P4-diguanosine 5′-tetraphosphate (Gp4G) has been isolated and purified from yolk platelets of encysted embryos of the brine shrimp, Artemia salina. The enzyme GTP:GTP guanylyltransferase (Gp4G synthetase) utilizes GTP as substrate, has a pH optimum of 5.9–6.0, a temperature optimum of 40–42 °C, and requires Mg2+ and dithiothreitol for optimal activity. The synthesis of Gp4G is inhibited markedly by pyrophosphate, whereas orthophosphate has no effect on the reaction. In the presence of GDP the enzyme also catalyzes the synthesis of P1,P3-diguanosine 5′-triphosphate (Gp3G), but the rate of synthesis is low compared with Gp4G synthesis and dependent upon other small molecular weight components of yolk platelets.


1973 ◽  
Vol 58 (3) ◽  
pp. 643-649 ◽  
Author(s):  
H. Schmitt ◽  
H. Grossfeld ◽  
U. Z. Littauer

Mitochondria isolated from cysts of Artemia salina (brine shrimp) were found to be devoid of cristae and to possess a low respiratory capability. Hydration of the cysts induces marked biochemical and morphological changes in the mitochondria. Their biogenesis proceeds in two stages. The first stage is completed within 1 h and is characterized by a rapid increase in the respiratory capability of the mitochondria, their cytochrome oxidase, cytochrome b, cytochrome c and perhaps some morphological changes. In the second stage there is an increase in the protein-synthesizing capacity of the mitochondria as well as striking changes in mitochondrial morphology leading to the formation of cristae.


2021 ◽  
Vol 14 (4) ◽  
pp. 702-716
Author(s):  
Thaiz Batista Azevedo Rangel Miguel ◽  
Elaine Cristina Maciel Porto ◽  
Sergimar Kennedy de Paiva Pinheiro ◽  
Emilio de Castro Miguel ◽  
Fabiano André Narciso Fernandes ◽  
...  

1978 ◽  
Vol 234 (5) ◽  
pp. R216-R222 ◽  
Author(s):  
D. Russler ◽  
J. Mangos

The osmoregulation of the nauplius of the brine shrimp, Artemia salina, was investigated using micropuncture and microanalytical techniques. The naupliar body fluid, hemolymph was hyposmotic to and had lower Na concentrations than the suspending medium for the range of medium salinities from 80 to 4,900 mM NaCl. In medium containing 20 mM NaCl, the hemolymph was hyperosmotic to the medium, with osmolarity of 101 +/- 8 mosmol/1 and with [Na] of 49 +/- 11 meq/1. Whereas the maximal observed NaCl concentration gradient between hemolymph and medium was 4,785 mM, during the incubation of nauplii in artificial seawater (osmolarity: 932 mosmol/1; and [Na]: 502 meq/1) the osmolarity and [Na] of the naupliar hemolymph were 161 +/- SD 16 mosmol/1 and 86 +/- 14 meq/1, respectively. The influx and efflux of Na between medium and hemolymph were measured using 22Na. The fluxes of this ion were temperature dependent. The main site of efflux of 22Na was the neck organ as was shown by experiments of differential recovery of 22Na introduced in the hemolymph. These studies demonstrate that the nauplius of A. salina has the ability to osmoregulate not only against high environmental salinities but also against low salinities approaching those of freshwater.


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