Growth Hormone Gene Transfer in Atlantic Salmon: Use of Fish Antifreeze/Growth Hormone Chimeric Gene Construct

Six transgenic pigs have been produced by microinjecting a human metallothionein promoter/porcine growth hormone gene construct into the pronuclei of fertilized eggs which were transferred to synchronized recipient sows. The resulting transgenic animals contained between 0.5 and 15 copies of the gene construct per cell, and at least one of the animals expressed the introduced gene and grew at an increased rate compared to both transgenic and non-transgenic littermates. Some of the transgenic animals that did not appear to grow at increased rates were found to contain rearranged gene sequences. Two of the transgenic pigs have been shown to pass on the introduced genes to their offspring.

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The complete nucleotide sequence of the growth-hormone gene from Atlantic salmon (Salmo salar)

Gene ◽

10.1016/0378-1119(89)90079-6 ◽

1989 ◽

Vol 77 (2) ◽

pp. 317-324 ◽

Cited By ~ 66

Author(s):

Berit Johansen ◽

Ole Christian Johnsen ◽

Svein Valla

Keyword(s):

Growth Hormone ◽

Nucleotide Sequence ◽

Atlantic Salmon ◽

Salmo Salar ◽

Complete Nucleotide Sequence ◽

Growth Hormone Gene ◽

Atlantic Salmon Salmo Salar

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Human growth hormone gene transfer into tumor cells may improve cancer chemotherapy

Cancer Gene Therapy ◽

10.1038/sj.cgt.7700467 ◽

2002 ◽

Vol 9 (6) ◽

pp. 497-504 ◽

Cited By ~ 7

Author(s):

C Cherbonnier ◽

O Déas ◽

G Vassal ◽

J L Merlin ◽

A Haeffner ◽

...

Keyword(s):

Growth Hormone ◽

Gene Transfer ◽

Tumor Cells ◽

Human Growth Hormone ◽

Cancer Chemotherapy ◽

Human Growth ◽

Growth Hormone Gene ◽

Human Growth Hormone Gene

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377: An innovative chimeric gene construct targeting p21 gene transfer into T lymphocytes

The Journal of Heart and Lung Transplantation ◽

10.1016/j.healun.2006.11.398 ◽

2007 ◽

Vol 26 (2) ◽

pp. S195

Author(s):

A.K. Khanna ◽

M.R. Mehra

Keyword(s):

Gene Transfer ◽

T Lymphocytes ◽

Chimeric Gene ◽

Gene Construct

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Coho salmon (Oncorhynchus kisutch) transgenic for a growth hormone gene construct exhibit increased rates of muscle hyperplasia and detectable levels of differential gene expression

Canadian Journal of Fisheries and Aquatic Sciences ◽

10.1139/f00-015 ◽

2000 ◽

Vol 57 (5) ◽

pp. 939-950 ◽

Cited By ~ 50

Author(s):

James A Hill ◽

Anders Kiessling ◽

Robert H Devlin

Keyword(s):

Gene Expression ◽

Growth Hormone ◽

Differential Gene Expression ◽

Coho Salmon ◽

Oncorhynchus Kisutch ◽

Transgenic Fish ◽

Growth Hormone Gene ◽

Gene Construct ◽

Muscle Hyperplasia ◽

Differential Gene

Transgenic coho salmon (Oncorhynchus kisutch) containing a growth hormone gene construct were compared with nontransgenic coho salmon in terms of gross anatomy, muscle cellularity, muscle enzyme activity, and differential gene expression. Transgenic fish were found to have significantly higher numbers of small-diameter muscle fibres in both the dorsal and lateral region of the somitic muscle, suggesting that they grow by greater rates of hyperplasia relative to slower growing nontransgenic fish. Higher levels of activity were found for phosphofructokinase and cytochrome oxidase in white muscle of the transgenic fish. This difference indicates a higher glycolytic and aerobic requirement in the muscle of transgenic fish. Subtractive hybridisation of muscle RNA of transgenic fish from control fish provided a library of cDNAs whose expression is upregulated in the transgenic fish. This library contains genes that may be involved in, or related to, both high growth rates and muscle hyperplasia. We have sequenced a number of fragments and have found a preponderance of myosin light chain 2 mRNAs, consistent with a putative high level of expression in the early stages of muscle fibre construction.

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Normalization of growth in hypophysectomized mice using hydrodynamic transfer of the human growth hormone gene

AJP Endocrinology and Metabolism ◽

10.1152/ajpendo.00573.2002 ◽

2003 ◽

Vol 285 (2) ◽

pp. E427-E432 ◽

Cited By ~ 18

Author(s):

Morten Søndergaard ◽

Frederik Dagnæs-Hansen ◽

Allan Flyvbjerg ◽

Thomas G. Jensen

Keyword(s):

Growth Hormone ◽

Gene Transfer ◽

Human Growth Hormone ◽

Body Weight Gain ◽

Tail Length ◽

Human Growth ◽

Longitudinal Growth ◽

Hormone Deficiency ◽

Heart Weight ◽

Growth Hormone Gene

Nonviral gene transfer was investigated as a potential treatment of growth hormone deficiency (GHD) using hypophysectomized mice as a model. After a single hydrodynamic administration of naked plasmid DNA containing the human growth hormone (hGH) gene controlled by an ubiquitin promoter, sustained elevation of circulating hGH was observed the entire observation period (68 days), with a concomitant normalization of circulating insulin-like growth factor I (IGF-I) and IGF-binding protein-3. Furthermore, longitudinal growth was corrected in terms of normalization of tibia length, tail length, and body weight gain. Liver, spleen, and lung weights were normalized, whereas heart weight was normalized partly. hGH mRNA was expressed exclusively in liver tissue. In conclusion, we showed that nonviral hGH gene transfer normalizes longitudinal growth in hypophysectomized mice, indicating that this method potentially could be relevant as a new therapeutic tool in the clinical handling of GHD.

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