Introduction of a porcine growth hormone fusion gene into transgenic pigs promotes growth

1988 ◽  
Vol 90 (2) ◽  
pp. 295-300
Author(s):  
P.D. Vize ◽  
A.E. Michalska ◽  
R. Ashman ◽  
B. Lloyd ◽  
B.A. Stone ◽  
...  
Keyword(s):  
Growth Hormone ◽  
Fusion Gene ◽  
Transgenic Animals ◽  
Growth Hormone Gene ◽  
Gene Sequences ◽  
Transgenic Pigs ◽  
Gene Construct ◽  
Metallothionein Promoter

Six transgenic pigs have been produced by microinjecting a human metallothionein promoter/porcine growth hormone gene construct into the pronuclei of fertilized eggs which were transferred to synchronized recipient sows. The resulting transgenic animals contained between 0.5 and 15 copies of the gene construct per cell, and at least one of the animals expressed the introduced gene and grew at an increased rate compared to both transgenic and non-transgenic littermates. Some of the transgenic animals that did not appear to grow at increased rates were found to contain rearranged gene sequences. Two of the transgenic pigs have been shown to pass on the introduced genes to their offspring.

Prenatal lethalities in mice homozygous for human growth hormone gene sequences integrated in the germ line

Cell ◽  
1983 ◽  
Vol 35 (3) ◽  
pp. 647-655 ◽  
Author(s):  
Erwin F. Wagner ◽  
Luis Covarrubias ◽  
Timothy A. Stewart ◽  
Beatrice Mintz
Keyword(s):  
Growth Hormone ◽  
Human Growth Hormone ◽  
Germ Line ◽  
Human Growth ◽  
Growth Hormone Gene ◽  
Gene Sequences ◽  
Human Growth Hormone Gene

scholarly journals Analysis of growth hormone gene sequences and polymorphisms in the Mongolian horse

2009 ◽  
Vol 80 (1) ◽  
pp. 1-6 ◽  
Author(s):  
Hong HAI ◽  
Emiko FUKUI ◽  
Telhisa HASEGAWA ◽  
Hiromichi MATSUMOTO ◽  
Harutaka MUKOYAMA ◽  
...  
Keyword(s):  
Growth Hormone ◽  
Growth Hormone Gene ◽  
Gene Sequences ◽  
Mongolian Horse

scholarly journals Coho salmon (Oncorhynchus kisutch) transgenic for a growth hormone gene construct exhibit increased rates of muscle hyperplasia and detectable levels of differential gene expression

2000 ◽  
Vol 57 (5) ◽  
pp. 939-950 ◽  
Author(s):  
James A Hill ◽  
Anders Kiessling ◽  
Robert H Devlin
Keyword(s):  
Gene Expression ◽  
Growth Hormone ◽  
Differential Gene Expression ◽  
Coho Salmon ◽  
Oncorhynchus Kisutch ◽  
Transgenic Fish ◽  
Growth Hormone Gene ◽  
Gene Construct ◽  
Muscle Hyperplasia ◽  
Differential Gene

Transgenic coho salmon (Oncorhynchus kisutch) containing a growth hormone gene construct were compared with nontransgenic coho salmon in terms of gross anatomy, muscle cellularity, muscle enzyme activity, and differential gene expression. Transgenic fish were found to have significantly higher numbers of small-diameter muscle fibres in both the dorsal and lateral region of the somitic muscle, suggesting that they grow by greater rates of hyperplasia relative to slower growing nontransgenic fish. Higher levels of activity were found for phosphofructokinase and cytochrome oxidase in white muscle of the transgenic fish. This difference indicates a higher glycolytic and aerobic requirement in the muscle of transgenic fish. Subtractive hybridisation of muscle RNA of transgenic fish from control fish provided a library of cDNAs whose expression is upregulated in the transgenic fish. This library contains genes that may be involved in, or related to, both high growth rates and muscle hyperplasia. We have sequenced a number of fragments and have found a preponderance of myosin light chain 2 mRNAs, consistent with a putative high level of expression in the early stages of muscle fibre construction.

The production of transgenic domestic livestock: successes, failures and the need for nuclear transfer

1998 ◽  
Vol 10 (8) ◽  
pp. 659 ◽  
Author(s):  
Kevin A. Ward ◽  
Bruce W. Brown
Keyword(s):  
Growth Hormone ◽  
Recombinant Dna ◽  
Genetic Manipulation ◽  
Good Health ◽  
Practical Method ◽  
Intermediary Metabolism ◽  
Growth Hormone Gene ◽  
Metallothionein Promoter ◽  
Domestic Livestock ◽  
Species Specific

The direct transfer of recombinant DNA to embryos is conceptually a powerful method for the manipulation of the genetic potential of domestic animals, but in practice the technology has yet to fulfil its promise. In this paper, two examples are given of research utilising direct genetic manipulation, both of which are aimed at increasing aspects of productivity in sheep. The first of these involves the modification of the growth hormone status of sheep by the use of the ovine growth hormone gene, the regulation of which has been altered by the use of an ovine metallothionein promoter. While there has been a large amount of research already conducted in this area by many groups, our recent results provide one of the first demonstrations that this approach can increase the growth rates of sheep while maintaining the animals in good health. The second project involves the modification of intermediary metabolism in sheep by the introduction of the cysteine biosynthetic pathway. The results to date demonstrate that it is possible to change intermediary metabolism in animals using our approach but that there are species-specific requirements that must be satisfied in order to make the approach a practical method for improving animal productivity.

Lipid composition of carcass tissue from transgenic pigs expressing a bovine growth hormone gene

1994 ◽  
Vol 72 (5) ◽  
pp. 1242-1246 ◽  
Author(s):  
M. B. Solomon ◽  
V. G. Pursel ◽  
E. W. Paroczay ◽  
D. J. Bolt
Keyword(s):  
Growth Hormone ◽  
Lipid Composition ◽  
Bovine Growth Hormone ◽  
Growth Hormone Gene ◽  
Transgenic Pigs

Growth Hormone Gene Transfer in Atlantic Salmon: Use of Fish Antifreeze/Growth Hormone Chimeric Gene Construct

1992 ◽  
pp. 176-189 ◽  
Author(s):  
Shao Jun Du ◽  
Zhiyuan Gong ◽  
Garth L. Fletcher ◽  
Margaret A. Shears ◽  
Choy L. Hew
Keyword(s):  
Growth Hormone ◽  
Gene Transfer ◽  
Atlantic Salmon ◽  
Chimeric Gene ◽  
Growth Hormone Gene ◽  
Gene Construct

The rat elastase I regulatory element is an enhancer that directs correct cell specificity and developmental onset of expression in transgenic mice

1987 ◽  
Vol 7 (8) ◽  
pp. 2956-2967
Author(s):  
R E Hammer ◽  
G H Swift ◽  
D M Ornitz ◽  
C J Quaife ◽  
R D Palmiter ◽  
...  
Keyword(s):  
Fusion Gene ◽  
Regulatory Element ◽  
Regulatory Region ◽  
Human Growth ◽  
Transgenic Animals ◽  
Growth Hormone Gene ◽  
Flanking Sequence ◽  
Transcriptional Enhancer ◽  
Independent Manner ◽  
Specific Expression

A total of 134 base pairs of the 5' flanking sequence of the elastase I gene is sufficient and necessary to direct expression of the passive human growth hormone gene (hGH) to the exocrine pancreas. We demonstrate that this elastase I regulatory region contains a transcriptional enhancer which directs acinar cell-specific expression in transgenic animals. The elastase I enhancer specifies correct expression of the linked hGH gene in an orientation- and position-independent manner and can activate a heterologous promoter. The enhancer also directs the appropriate temporal activation of the hGH gene in the developing pancreas. Transcription is initiated correctly for the elastase I or hGH promoter, and the transcripts are correctly processed regardless of the enhancer position within or outside the fusion gene. The elastase I enhancer generates coincident DNase I-hypersensitive sites in pancreatic chromatin when moved 3 kilobases upstream or within the first intron of the hGH gene and when associated with the hGH promoter.

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