Sulforaphane Induces Cell Death Through G2/M Phase Arrest and Triggers Apoptosis in HCT 116 Human Colon Cancer Cells

2016 ◽  
Vol 44 (06) ◽  
pp. 1289-1310 ◽  
Author(s):  
Kuo-Ching Liu ◽  
Ting-Ying Shih ◽  
Chao-Lin Kuo ◽  
Yi-Shih Ma ◽  
Jiun-Long Yang ◽  
...  

Sulforaphane (SFN), an isothiocyanate, exists exclusively in cruciferous vegetables, and has been shown to possess potent antitumor and chemopreventive activity. However, there is no available information that shows SFN affecting human colon cancer HCT 116 cells. In the present study, we found that SFN induced cell morphological changes, which were photographed by contrast-phase microscopy, and decreased viability. SFN also induced G2/M phase arrest and cell apoptosis in HCT 116 cells, which were measured with flow cytometric assays. Western blotting indicated that SFN increased Cyclin A, cdk 2, Cyclin B and WEE1, but decreased Cdc 25C, cdk1 protein expressions that led to G2/M phase arrest. Apoptotic cell death was also confirmed by Annexin V/PI and DAPI staining and DNA gel electrophoresis in HCT 116 cells after exposure to SFN. The flow cytometric assay also showed that SFN induced the generation of reactive oxygen species (ROS) and Ca[Formula: see text] and decreased mitochondria membrane potential and increased caspase-8, -9 and -3 activities in HCT 116 cell. Western blotting also showed that SFN induced the release of cytochrome c, and AIF, which was confirmed by confocal microscopy examination. SFN induced ER stress-associated protein expression. Based on those observations, we suggest that SFN may be used as a novel anticancer agent for the treatment of human colon cancer in the future.

2007 ◽  
Vol 30 (9) ◽  
pp. 1779-1783 ◽  
Author(s):  
Kazuhiro Ishizu ◽  
Naohide Sunose ◽  
Kanami Yamazaki ◽  
Takashi Tsuruo ◽  
Sotaro Sadahiro ◽  
...  

2020 ◽  
Vol 44 (2) ◽  
pp. 768-776 ◽  
Author(s):  
Jinlu Zhao ◽  
Guodong Li ◽  
Jiufeng Wei ◽  
Shuwei Dang ◽  
Xiaotong Yu ◽  
...  

2021 ◽  
Vol 0 (0) ◽  
pp. 0
Author(s):  
Bedia Cakmakoglu ◽  
BesteTacal Aslan ◽  
Baris Ertugrul ◽  
ElifSinem Iplik

2016 ◽  
Vol 26 ◽  
pp. 1-10 ◽  
Author(s):  
Sara Jaramillo ◽  
Francisco J.G. Muriana ◽  
Rafael Guillen ◽  
Ana Jimenez-Araujo ◽  
Rocio Rodriguez-Arcos ◽  
...  

2017 ◽  
Vol 4 (S) ◽  
pp. 104 ◽  
Author(s):  
Po-Yu Lai ◽  
Shu-Chen Hsieh ◽  
Chih-Chung Wu ◽  
Shu-Ling Hsieh

Colorectal cancer is the third most commonly diagnosed cancer in the word. Carnosine is an endogenous dipeptide found in vertebrate skeletal muscles. It is known to have anti-fatigue, antioxidative, antihypertensive, antidiabetic, and cancer inhibiting effects. However, little research has been done regarding its influence on the metastasis of colon cancer. This study cultivated HCT-116 human colon cancer cells as a test model in order to investigate the impact of carnosine on the migration and invasion of human colon cancer cells. The results showed that 48-hour treatments of HCT-116 cells with 0.5, 1, or 5 mM carnosine each significantly inhibited the migration ability of the cells (P < 0.05). The 48-hour treatments with 0.5, 1, or 5 mM carnosine were also found to significantly reduce MMP-9 activity (P < 0.05), but not MMP-2 expression. Furthermore, when HCT-116 cells treated with 1 or 5 mM carnosine, invasion ability are significantly decreased and significantly increased E-cadherin expression (P < 0.05). On the other hand, the protein of TIMP-1, an inhibitor of MMP-9, is signification increased after 1 or 5 mM carnosine treatment (P<0.05). In addition, the u-PA protein level are significantly decreased after carnosine treatment. The results indicate that carnosine can regulate the migration and invasion by regulating MMPs and its regulator molecular expression in HCT-116 cells.


2021 ◽  
Vol 177 ◽  
pp. S75
Author(s):  
Ana Obradović ◽  
Miloš Matić ◽  
Branka Ognjanović ◽  
Emilija Marinković ◽  
Bojan Božić ◽  
...  

2004 ◽  
Vol 216 (2) ◽  
pp. 175-181 ◽  
Author(s):  
Ge Li ◽  
Dong-Hee Kim ◽  
Tae-Dong Kim ◽  
Byoung-Jeon Park ◽  
Hae-Duck Park ◽  
...  

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