Protective and Therapeutic Effects of Huanglian-Jie-Du-Tang on Hepatotoxin-induced Liver Injuries

1996 ◽  
Vol 24 (03n04) ◽  
pp. 219-229 ◽  
Author(s):  
Song-Chow Lin ◽  
Chun-Ching Lin ◽  
Fung-Jou Lu ◽  
Yun-Ho Lin ◽  
Ching-Hsein Chen
Keyword(s):  
Carbon Tetrachloride ◽  
Experimental Models ◽  
Hepatoprotective Effect ◽  
Therapeutic Effects ◽  
Liver Injuries ◽  
Serum Glutamate Pyruvate Transaminase ◽  
Glutamate Pyruvate ◽  
Hepatoprotective Effects ◽  
Experimental Liver ◽  
Serum Glutamate

The hepatoprotective effect of Huanglian-Jie-Du-Tang (HLJDT), a Chinese medicinal prescription, was investigated in three kinds of experimental models. The animals were treated with HLJDT (300 mg/kg, p.o.) thrice at 2, 4 and 10 hours after administration with carbon tetrachloride (32 μl/kg, i.p.), acetaminophen (600 mg/kg, i.p.) and β-D-galactosamine (188 mg/kg, i.p.). Significant hepatoprotective effects on carbon tetrachloride and actaminophen induced liver injuries were noted, but no significant effect on β-D-galactosamine induced liver injury was observed. These hepatoprotective effects were evidenced by comparing the serum glutamate oxaloacetate transaminase (SGOT) and serum glutamate pyruvate transaminase (SGPT) levels in HLJDT treated and untreated groups. Serum enzyme activities in the carbon tetrachloride and acetaminophen experiments were significantly lower in the treated groups while the herbal prescription has no effect on the β-D-galactosamine experiment. These results demonstrated that Huanglian-Jie-Du-Tang has a hepatoprotective effect against experimental liver injuries induced by specific hepatotoxins, and therefore may be useful in treating some, but not all, liver injuries.

Protective and Therapeutic Effects of Ban-zhi-lian on Hepatotoxin-induced Liver Injuries

1994 ◽  
Vol 22 (01) ◽  
pp. 29-42 ◽  
Author(s):  
Song-chow Lin ◽  
Chun-ching Lin ◽  
Yun-ho Lin ◽  
Ching-hsein Chen
Keyword(s):  
Liver Damage ◽  
Experimental Models ◽  
Therapeutic Effects ◽  
Protective Effects ◽  
Liver Injuries ◽  
Serum Glutamate Pyruvate Transaminase ◽  
Glutamate Pyruvate ◽  
Experimental Liver ◽  
Serum Glutamate ◽  
Experimental Liver Damage

The hepatoprotective effect of Ban-zhi-lian was investigated in three kinds of experimental models. The animals were treated with Ban-zhi-lian (300 mg/kg, p.o.) at 2, 4, and 10 hours after carbon tetrachloride (32l/kg, i.p.), acetaminophen (600 mg/kg, i.p.), and β-D-galactosamine (188 mg/kg, i.p.) administration. Significant protective effects from these hepatotoxins were expressed. This protection was evidenced by comparing the serum glutamate oxaloacetate transaminase (SGOT), serum glutamate pyruvate transaminase (SGPT), and histopathologic examination in animals treated and untreated with Ban-zhi-lian. Serum enzyme activities were significantly lower in Ban-zhi-lian-treated groups. In the histopathologic observation, liver damage induced by three hepatotoxins was markedly improved in Ban-zhi-lian treated animals. These results demonstrated that Ban-zhi-lian has a protective effect against experimental liver damage induced by various hepatotoxins.

Hepatoprotective Effects of Taiwan Folk Medicine: Wedelia chinensis on Three Hepatotoxin-induced Hepatotoxicity

1994 ◽  
Vol 22 (02) ◽  
pp. 155-168 ◽  
Author(s):  
Song-chow Lin ◽  
Chung-ching Lin ◽  
Yun-ho Lin ◽  
Shyh-jong Shyuu
Keyword(s):  
Carbon Tetrachloride ◽  
Microscopic Examination ◽  
Marked Improvement ◽  
Folk Medicine ◽  
Hepatoprotective Effect ◽  
Liver Injuries ◽  
Hepatoprotective Effects ◽  
Serum Glutamate ◽  
Histopathological Effects ◽  
Glutamate Oxaloacetic Transaminase

The hepatoprotective effects of a Taiwanese crude herb, Hwang-hua-mih-tsay (Wedelia chinensis (Osbeck) Merr.), were investigated. Acute hepatitis was induced by three hepatotoxins: carbon tetrachloride and acetaminophen in mice, and D(+)-galactosamine in rats. After treatment with W. chinensis (300 mg/kg, p.o.) at 2,6 and 10 hours, a reduction in the elevation of serum glutamate oxaloacetic transaminase (SGOT) and glutamate pyruvic transaminase (SGPT) levels was observed at 24 hrs after hepatotoxins were administered. These serological observations were confirmed by histopathological examinations. A microscopic examination of the liver showed a marked improvement in groups receiving W. chinensis. In order to further confirm the hepatoprotective effect of W. chinensis, all pharmacological and histopathological effects were compared with Bupleurum chinense DC. (family Umbelliferae), a well documented antihepatotoxicity herb. It was concluded that W. chinensis has a definite hepatoprotective effect against liver injuries.

scholarly journals Antidiabetics in combination with hydroxychloroquine improve antioxidant and hepatoprotective activities in alloxan-induced diabetic rats

2015 ◽  
Vol 18 (1) ◽  
pp. 72-77
Author(s):  
Shaheda Zannah ◽  
Monirul Islam ◽  
Yusuf Ali ◽  
Md Asaduzzaman ◽  
Md Shahid Sarwar ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Pharmaceutical Journal ◽  
Diabetic Rats ◽  
Hepatoprotective Effect ◽  
Sod Activity ◽  
Pancreatic Cells ◽  
Additional Protection ◽  
Serum Glutamate Pyruvate Transaminase ◽  
Glutamate Pyruvate ◽  
Serum Glutamate

Hyperglycemia exerts toxic effects on the pancreatic ?-cells. This study investigated the hypothesis that the antidiabetic drugs glibenclamide and metformin, in combination with hydroxychloroquine (HCQ) offer additional protection for the pancreas against oxidative stress and produce hepatoprotective effect in alloxan-induced diabetic rats. Diabetes was induced in male Long-Evans rats by a single dose of alloxan (120 mg/kg; i.p.). Different groups of diabetic animals were treated with glibenclamide (10 mg/70 kg, i.p.), metformin (850 mg/70 kg, i.p.), HCQ (300 mg/70 kg, i.p.) and combination of both glibenclamide and metformin with HCQ, separately for a period of 28 days. Diabetic rats had significantly elevated levels of serum glutamate oxaloacetate transaminase (SGOT) and serum glutamate pyruvate transaminase (SGPT), while catalase (CAT) and superoxide dismutase (SOD) activity were significantly reduced. Glibenclamide and metformin produced no significant effects on antioxidant enzymes but both showed significant (p<0.05) result in reducing SGOT and SGPT level in diabetic rats. In contrast, the combination of glibenclamide or metformin with HCQ showed better effect on up-regulation of CAT and SOD activity and down-regulation of SGOT and SGPT activity in comparison with the antidiabetic drug alone. These findings suggest that, HCQ potentiates the effect of glibenclamide and metformin to protect pancreas against oxidative stress and produce hepatoprotective effect in diabetic rats.Bangladesh Pharmaceutical Journal 18(1): 72-77, 2015

The Effectiveness of Tsu-San-Li (St-36) and Tai-Chung (Li-3) Acupoints for Treatment of Acute Liver Damage in Rats

2001 ◽  
Vol 29 (02) ◽  
pp. 221-226 ◽  
Author(s):  
Hsu-Jan Liu ◽  
Sheng-Feng Hsu ◽  
Chang-Chi Hsieh ◽  
Tin-Yun Ho ◽  
Ching-Liang Hsieh ◽  
...  
Keyword(s):  
Carbon Tetrachloride ◽  
Acupuncture Therapy ◽  
Liver Injury ◽  
Acute Liver Injury ◽  
Male Rats ◽  
Acute Liver Damage ◽  
Glutamate Pyruvate Transaminase ◽  
Serum Glutamate Pyruvate Transaminase ◽  
Glutamate Pyruvate ◽  
Serum Glutamate

This study investigated the effects of acupuncture on carbon tetrachloride (CCl4) induced acute liver injury in male rats (n = 36). The experimental groups were injected with CCl4 before, during, or after acupuncture therapy. Acupoints similar to the human Tsu-San-Li (St-36) and Tai-Chung (Li-3) were needled bilaterally. Rats treated with CCl4 had higher levels of serum glutamate-oxalate-transaminase (sGOT) and serum glutamate-pyruvate-transaminase (sGPT). Comparing the experimental groups, biochemical and pathological parameters of liver injury were significantly reduced when rats were acupunctured after, not before, CCl4-induced hepatotoxicity. Acupuncture at the Tsu-Sa-Li and Tai-Chung acupoints cannot prevent acute liver injury but may be effective in treating liver injury induced by carbon tetrachloride in rats.

Actinidia rubricaulisAttenuates Hepatic Fibrosis Induced by Carbon Tetrachloride in Rats

2007 ◽  
Vol 35 (01) ◽  
pp. 81-88 ◽  
Author(s):  
Jung-Chun Liao ◽  
Kun-Hung Lin ◽  
Hao-Yuan Cheng ◽  
Jin-Bin Wu ◽  
Ming-Tsuen Hsieh ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Carbon Tetrachloride ◽  
Liver Injury ◽  
Experimental Period ◽  
Hepatoprotective Effect ◽  
Glutamate Pyruvate Transaminase ◽  
Chronic Liver Injury ◽  
Glutamate Pyruvate ◽  
Ethanol Extracts ◽  
Serum Glutamate

The purpose of this study was to evaluate the antioxidant activity and hepatoprotective effect of ethanol extracts of Actinidia rubricaulis (AR) on chronic liver injury induced by carbon tetrachloride ( CCl4) in rats. CCl4(20%, 0.5 ml/rat) was given twice a week for 8 weeks, and animals received AR throughout the entire experimental period. AR reduced the elevated levels of serum glutamate-oxalate-transaminase (sGOT) and glutamate-pyruvate-transaminase (sGPT) caused by CCl4at weeks 1,3,6, and 8. The biochemical data were consistent with those of the histological observations. The AR extract recovered the CCl4-induced liver injury and showed antioxidant effect in assays of antioxidant enzyme activity, such as SOD, GSH-Px and GSH-Rd. Based on these results, we suggest that the hepatoprotective effect of the AR is related to its antioxidant activity.

scholarly journals Hepatoprotective Effect of Hovenia dulcis THUNB. on Experimental Liver Injuries Induced by Carbon Tetrachloride or D-Galactosamine/Lipopolysaccharide.

1997 ◽  
Vol 20 (4) ◽  
pp. 381-385 ◽  
Author(s):  
Koji HASE ◽  
Mizue OHSUGI ◽  
Quanbo XIONG ◽  
Purusotam BASNET ◽  
Shigetoshi KADOTA ◽  
...  
Keyword(s):  
Carbon Tetrachloride ◽  
Hepatoprotective Effect ◽  
Liver Injuries ◽  
Experimental Liver ◽  
Hovenia Dulcis

scholarly journals Effect of garlic extract (Allium sativum) on hepatic microanatomy structure and serum glutamate-pyruvate transaminase levels in rats after oral treatment of CCl4

2006 ◽  
Vol 4 (2) ◽  
pp. 72-78 ◽  
Author(s):  
WIWIK YULIA TRISTININGRUM ◽  
MARTI HARINI ◽  
TETRI WIDIYANI
Keyword(s):  
Carbon Tetrachloride ◽  
Allium Sativum ◽  
Oral Treatment ◽  
Garlic Extract ◽  
Central Vein ◽  
Glutamate Pyruvate Transaminase ◽  
Wistar Strain ◽  
Serum Glutamate Pyruvate Transaminase ◽  
Glutamate Pyruvate ◽  
Serum Glutamate

Garlic (Allium sativum L.) is known to have various pharmacological activities, such as antidiabetic, antihypertensive, anti-cholesterol, anti-atherosclerosis, thrombocyte antiaggregation, initiate fibrinolysis, antiviral and antimicrobial, anticancer and antioxidant. The objectives of this research were to determine the effect of garlic extract (Allium sativum L.) on the microanatomy structure of liver and the serum glutamate-pyruvate transaminase (SGPT) levels in rats (Rattus norvegicus L.) after the oral treatment of carbon tetrachloride (CCl4). The study was conducted by using 25 Wistar strain rat aged 2-3 months with body weight 200-250 grams divided into five groups, each group consisted of 5 rats as replicates which kept under the same conditions and treated for 28 days. The results showed that the treatment of garlic extract had an effect on the improvement of microanatomy structure of damaged liver cells in rats after oral treatment of carbon tetrachloride (CCl4). The damage was in the form of fatty liver, pyknotic, karyorrhexis, karyolysis and central vein dilatation. The treatment of garlic extract also reduced the serum pyruvate-transaminase glutamate (SGPT) levels in rats after the oral treatment of carbon tetrachloride (CCl4).

scholarly journals Hepatoprotective Effects of Thespesia lampas Dalz & Gibs in CCl4 Induced Liver Injury in Rats

1970 ◽  
Vol 7 (1) ◽  
pp. 11-13 ◽  
Author(s):  
B Sangameswaran ◽  
Chumbhale Deshraj ◽  
BR Balakrishnan ◽  
B Jayakar
Keyword(s):  
Carbon Tetrachloride ◽  
Tween 80 ◽  
Treated Group ◽  
Hepatoprotective Activity ◽  
Glutamate Oxaloacetate Transaminase ◽  
Glutamate Pyruvate Transaminase ◽  
Chronic Liver Damage ◽  
Serum Glutamate Pyruvate Transaminase ◽  
Glutamate Pyruvate ◽  
Serum Glutamate

The extracts of the roots of Thespesia lampas (Malvaceae) were evaluated for hepatoprotective activity in rats by inducing chronic liver damage by subcutaneous injection of 50% v/v carbon tetrachloride in Tween 80 at a dose of 3ml/kg for a period of 4 weeks. The biochemical parameters like serum glutamate pyruvate transaminase (SGPT), serum glutamate oxaloacetate transaminase (SGOT), alkaline phosphatase (ALP), serum bilirubin and total proteins were estimated to assess the liver function. Hepatic steatosis, centrilobular necrosis, and often swelling of the hepatic cytoplasm were observed in carbontetra chloride treated group, while these were completely absent in the extracts of T. lampas (300 mg/kg b.wt) treated groups (p < 0.01). The present investigation established pharmacological evidence to support the folkloric claim of hepatoprotective activity of T. lampas.Key words: Thespesia lampas, Carbon tetrachloride, Hepatoprotective, Rats, Root extracts  DOI = 10.3329/dujps.v7i1.1201Dhaka Univ. J. Pharm. Sci. 7(1): 11-13, 2008 (June)

Hepatoprotective effects of zingerone on carbon tetrachloride- and dimethylnitrosamine-induced liver injuries in rats

2015 ◽  
Vol 39 (2) ◽  
pp. 279-291 ◽  
Author(s):  
Kyoung Ook Cheong ◽  
Dong-Su Shin ◽  
Jeonghyeon Bak ◽  
Changyong Lee ◽  
Kyung Wook Kim ◽  
...  
Keyword(s):  
Carbon Tetrachloride ◽  
Liver Injuries ◽  
Hepatoprotective Effects

scholarly journals Curative Role of Dithiophenolato Titanium (IV)-Chitosan Nanocomposite Complex Against Carbon Tetrachloride-Induced Liver Injuries

2021 ◽  
Author(s):  
Nadia Z Shaban ◽  
Salah A Yehiaa ◽  
Doaa Awad ◽  
Shaban Y Shaban ◽  
Samar R Saleh
Keyword(s):  
Oxidative Stress ◽  
Carbon Tetrachloride ◽  
Lipid Profile ◽  
Hepg2 Cell ◽  
Chitosan Nanoparticles ◽  
Antibacterial Activities ◽  
Therapeutic Effects ◽  
Liver Histopathology ◽  
Liver Injuries ◽  
Kidney Functions

Abstract Background Titanium-based compounds have been incorporated as promising antineoplastic metals. In our previous studies, dithiophenolato titanium (IV) Complex "DBT" and its chitosan nanocomposite "DBT-CSNPs" were prepared and we showed that these compounds have antibacterial activities, cytotoxic, and have abilities to bind with DNA helixes. Therefore, in this study, we evaluated the LD50 values of dithiophenolato titanium (IV)-complex (DBT) and its high thermal stable chitosan nanoparticles (DBT-CSNPs). Then their therapeutic effects against liver injuries induced by carbon tetrachloride (CCl4) were assessed and compared with cisplatin treatment. Additionally, the anti-proliferative activity of DBT and DBT-CSNPs against human liver cancer (HepG2) cell lines through the analysis of the cell cycle was evaluated. Methods Nine groups of rats were prepared: normal, DBT, DBT-CSNPs, CSNPs, CCl4, CCl4-DBT, CCl4-DBT-CSNPs, CCl4-CSNPs and CCl4-cisplatin. Liver histopathology and the biochemical markers involving oxidative stress, apoptosis, liver and kidney functions, and lipid profile were determined. Results The results revealed that the treatment with DBT-CSNPs and DBT after CCl4 administration abolished liver damage since it reduced the apoptosis induced by CCl4 via the reduction of DNA fragmentation, Bax and caspase- 8 with an elevation of Bcl2 and Bcl2/Bax ratio. Also, these treatments caused nonsignificant changes in the markers of oxidative stress. Therefore, liver histopathology and functions, lipid profile, and kidney functions were improved. Cisplatin treatment reduced liver injury with a degree less than DBT-CSNPs and DBT, but it induced nephrotoxicity. Administration of DBT-CSNPs and DBT to healthy rats for 14 days has no adverse effect. Also, the results showed that DBT-CSNPs and DBT inhibited the proliferation of HepG2 cells by arresting cells in the G2/M phase and inducing cell death. Conclusion DBT-CSNPs and DBT have a therapeutic effect against CCl4-induced liver injuries via the reduction of apoptosis induced by CCl4. Moreover, both compounds have antineoplastic activities against the HepG2 cell line. In all cases, DBT-CSNPs have a greater effect due to their nanostructure. Therefore, both compounds can be used in the pharmacological fields, particularly DBT-CSNPs.

Sign in / Sign up

Export Citation Format

Share Document