scholarly journals Antidiabetics in combination with hydroxychloroquine improve antioxidant and hepatoprotective activities in alloxan-induced diabetic rats

2015 ◽  
Vol 18 (1) ◽  
pp. 72-77
Author(s):  
Shaheda Zannah ◽  
Monirul Islam ◽  
Yusuf Ali ◽  
Md Asaduzzaman ◽  
Md Shahid Sarwar ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Pharmaceutical Journal ◽  
Diabetic Rats ◽  
Hepatoprotective Effect ◽  
Sod Activity ◽  
Pancreatic Cells ◽  
Additional Protection ◽  
Serum Glutamate Pyruvate Transaminase ◽  
Glutamate Pyruvate ◽  
Serum Glutamate

Hyperglycemia exerts toxic effects on the pancreatic ?-cells. This study investigated the hypothesis that the antidiabetic drugs glibenclamide and metformin, in combination with hydroxychloroquine (HCQ) offer additional protection for the pancreas against oxidative stress and produce hepatoprotective effect in alloxan-induced diabetic rats. Diabetes was induced in male Long-Evans rats by a single dose of alloxan (120 mg/kg; i.p.). Different groups of diabetic animals were treated with glibenclamide (10 mg/70 kg, i.p.), metformin (850 mg/70 kg, i.p.), HCQ (300 mg/70 kg, i.p.) and combination of both glibenclamide and metformin with HCQ, separately for a period of 28 days. Diabetic rats had significantly elevated levels of serum glutamate oxaloacetate transaminase (SGOT) and serum glutamate pyruvate transaminase (SGPT), while catalase (CAT) and superoxide dismutase (SOD) activity were significantly reduced. Glibenclamide and metformin produced no significant effects on antioxidant enzymes but both showed significant (p<0.05) result in reducing SGOT and SGPT level in diabetic rats. In contrast, the combination of glibenclamide or metformin with HCQ showed better effect on up-regulation of CAT and SOD activity and down-regulation of SGOT and SGPT activity in comparison with the antidiabetic drug alone. These findings suggest that, HCQ potentiates the effect of glibenclamide and metformin to protect pancreas against oxidative stress and produce hepatoprotective effect in diabetic rats.Bangladesh Pharmaceutical Journal 18(1): 72-77, 2015

scholarly journals Antidiabetic Activity ofArtemisia amygdalinaDecne in Streptozotocin Induced Diabetic Rats

2014 ◽  
Vol 2014 ◽  
pp. 1-10 ◽  
Author(s):  
Khalid Ghazanfar ◽  
Bashir A. Ganai ◽  
Seema Akbar ◽  
Khan Mubashir ◽  
Showkat Ahmad Dar ◽  
...  
Keyword(s):  
Diabetic Control ◽  
Diabetic Rats ◽  
Antidiabetic Activity ◽  
Traditional Use ◽  
Glucose Levels ◽  
Methanolic Extracts ◽  
Serum Glutamate Pyruvate Transaminase ◽  
Glutamate Pyruvate ◽  
Histopathological Results ◽  
Serum Glutamate

Artemisiaspecies have been extensively used for the management of diabetes in folklore medicine. The current study was designed to investigate the antidiabetic and antihyperlipidemic effects ofArtemisia amygdalina. Petroleum ether, ethyl acetate, methanol, and hydroethanolic extracts ofArtemisia amygdalinawere tested for their antidiabetic potentials in diabetic rats. The effect of extracts was observed by checking the biochemical, physiological, and histopathological parameters in diabetic rats. The hydroethanolic and methanolic extracts each at doses of 250 and 500 mg/kg b. w significantly reduced glucose levels in diabetic rats. The other biochemical parameters like cholesterol, triglycerides, low density lipoproteins (LDL), serum creatinine, serum glutamate pyruvate transaminase (SGPT), serum glutamate oxaloacetate transaminase (SGOT), and alkaline phosphatise (ALP), were found to be reduced by the hydroethanolic and methanolic extracts. The extracts also showed reduction in the feed and water consumption of diabetic rats when compared with the diabetic control. The histopathological results of treated groups showed the regenerative/protective effect onβ-cells of pancreas in diabetic rats. The current study revealed the antidiabetic potential ofArtemisia amygdalinabeing effective in hyperglycemia and that it can effectively protect against other metabolic aberrations caused by diabetes in rats, which seems to validate its therapeutic traditional use.

Protective and Therapeutic Effects of Huanglian-Jie-Du-Tang on Hepatotoxin-induced Liver Injuries

1996 ◽  
Vol 24 (03n04) ◽  
pp. 219-229 ◽  
Author(s):  
Song-Chow Lin ◽  
Chun-Ching Lin ◽  
Fung-Jou Lu ◽  
Yun-Ho Lin ◽  
Ching-Hsein Chen
Keyword(s):  
Carbon Tetrachloride ◽  
Experimental Models ◽  
Hepatoprotective Effect ◽  
Therapeutic Effects ◽  
Liver Injuries ◽  
Serum Glutamate Pyruvate Transaminase ◽  
Glutamate Pyruvate ◽  
Hepatoprotective Effects ◽  
Experimental Liver ◽  
Serum Glutamate

The hepatoprotective effect of Huanglian-Jie-Du-Tang (HLJDT), a Chinese medicinal prescription, was investigated in three kinds of experimental models. The animals were treated with HLJDT (300 mg/kg, p.o.) thrice at 2, 4 and 10 hours after administration with carbon tetrachloride (32 μl/kg, i.p.), acetaminophen (600 mg/kg, i.p.) and β-D-galactosamine (188 mg/kg, i.p.). Significant hepatoprotective effects on carbon tetrachloride and actaminophen induced liver injuries were noted, but no significant effect on β-D-galactosamine induced liver injury was observed. These hepatoprotective effects were evidenced by comparing the serum glutamate oxaloacetate transaminase (SGOT) and serum glutamate pyruvate transaminase (SGPT) levels in HLJDT treated and untreated groups. Serum enzyme activities in the carbon tetrachloride and acetaminophen experiments were significantly lower in the treated groups while the herbal prescription has no effect on the β-D-galactosamine experiment. These results demonstrated that Huanglian-Jie-Du-Tang has a hepatoprotective effect against experimental liver injuries induced by specific hepatotoxins, and therefore may be useful in treating some, but not all, liver injuries.

scholarly journals Hepatoprotective activity of Adina cordifolia against ethanol induce hepatotoxicity in rats

2012 ◽  
Vol 1 (9) ◽  
pp. 279-284 ◽  
Author(s):  
A Sharma ◽  
B Sangameswaran ◽  
V Jain ◽  
M S Saluja
Keyword(s):  
Pharmaceutical Journal ◽  
Hepatoprotective Activity ◽  
Serum Markers ◽  
Hepatocellular Injury ◽  
Standard Drug ◽  
Blood Samples ◽  
Hepatic Cells ◽  
Serum Glutamate Pyruvate Transaminase ◽  
Histopathological Studies ◽  
Serum Glutamate

The acetone (AEAC) and aqueous extracts (AQEAC) of Adina cordifolia, belonging to the family Rubiaceae, were studied for hepatoprotective activity against Wister rats with liver damage induced by ethanol. It was found that AEAC and AQEAC, at a dose of 500 mg/kg body weight exhibited hepatoprotective effect by lowering the Serum Glutamate Pyruvate Transaminase (SGPT), Serum Glutamate Oxaloacetate Transaminase (SGOT), alkaline phosphate and total bilirubin to a significant extent and also significantly increased the levels of total protein. The hepatoprotective activity was also supported by histopathological studies of liver tissue. Since results of biochemical studies of blood samples of ethanol treated rats showed significant increase in the levels of serum enzyme activities, reflecting the liver injury caused by ethanol and blood samples from the animals treated with AEAC and AQEAC showed significant decrease in the levels of serum markers, indicating the protection of hepatic cells against ethanol induced hepatocellular injury. The effects of AEAC and AQEAC were comparable with standard drug silymarin.DOI: http://dx.doi.org/10.3329/icpj.v1i9.11619 International Current Pharmaceutical Journal 2012, 1(9): 279-284 

scholarly journals Nutritional Status of Tuberculosis Patients in Against Serum Glutamate Pyruvate Transaminase Levels

2021 ◽  
Vol 1 (3) ◽  
pp. 14
Author(s):  
Iqbal Donarika Widagdo ◽  
Setyoko Setyoko ◽  
M. Riza Setiawan
Keyword(s):  
Nutritional Status ◽  
Rank Correlation ◽  
Test Results ◽  
Spearman Rank Correlation ◽  
Glutamate Pyruvate Transaminase ◽  
Tuberculosis Patients ◽  
Spearman Rank ◽  
Serum Glutamate Pyruvate Transaminase ◽  
Glutamate Pyruvate ◽  
Serum Glutamate

Background: Treatment of Tuberculosis patients are given in the form of a drugs combination. Some drugs which are Pyrazinamide, Isoniazid and Rifampicin can provide hepatotoxic side effects. Specific sign of hepatotoxic occurrence is increasing in the Serum Glutamate Pyruvate Transaminase levels. One of factors that influence hepatotoxicity is nutritional status. This study aims to determine the relationship between nutritional status and levels of Serum Glutamate Pyruvate Transaminase in tuberculosis patients.Methods: A non-experimental correlation study, retrospective approach with total sampling method, according to inclusion and exclusion criteria. Located at tCommunity Lung Health Center (BKPM) Ambarawa area. The analysis used the Spearman rank correlation testResults: Based on the test results of Spearman Rank correlation test, the correlation coefficient (r) is  -0.267. Hypothesis test results also showed 0.037 (p-value <0.05), which means the existence of a significant correlation between variabels.Conclusion: There is a relationship between nutritional status and levels of Serum Glutamate Pyruvate Transaminase in tuberculosis patients.

scholarly journals EVALUATION OF ANTI-HYPERTROPHIC POTENTIAL OF CAMELLIA SINENSIS IN ISOPROTERENOL INDUCED CARDIAC HYPERTROPHY

2018 ◽  
Vol 10 (10) ◽  
pp. 119
Author(s):  
Doss V. A. ◽  
Jeevitha Parthibhan ◽  
Dharaniyambigai Kuberapandian
Keyword(s):  
Lactate Dehydrogenase ◽  
Cardiac Hypertrophy ◽  
Aqueous Extract ◽  
Marker Enzymes ◽  
Glutamate Oxaloacetate Transaminase ◽  
Glutamate Pyruvate Transaminase ◽  
Cardiac Marker ◽  
Serum Glutamate Pyruvate Transaminase ◽  
Glutamate Pyruvate ◽  
Serum Glutamate

Objective: Camellia sinensis (C. sinensis family-Theaceae) has potent antioxidant activity used in the treatment of cardiovascular disease. The present study evaluates the cardioprotective (anti-hypertrophic) effect of aqueous extract of C. sinensis in isoproterenol (ISO) induced cardiac hypertrophic rats.Methods: The beneficial effect of the green tea extract was examined by the administration of the aqueous extract of the leaves of C. sinensis (100 mg/kg b.w., oral., 7 d) in ISO (10 mg/kg b.w., subcutaneous.,7 d) induced cardiac hypertrophic rats with reference to the standard drug, losartan (50 mg/kg b.w., oral.,7 d) followed by biochemical estimations of glucose, protein, cholesterol, cardiac marker enzymes namely serum glutamate oxaloacetate transaminase (SGOT), serum glutamate pyruvate transaminase (SGPT) and lactate dehydrogenase (LDH) in serum and heart tissues thus collected at the end of 7 d.Results: The biochemical assays revealed significantly (P<0.05) increased glucose, protein, cholesterol, cardiac marker enzymes namely serum glutamate oxaloacetate transaminase (SGOT), serum glutamate pyruvate transaminase (SGPT) lactate dehydrogenase (LDH) and significantly (P<0.05) decreased in ISO induced cardiac hypertrophic rats that were reciprocated by the effect of plant extract.Conclusion: Thus, this study showed that the aqueous leaf extract of C. sinensis possesses potent effect against cardiac hypertrophy. This potential is hypothesized to be due to the phytochemical, Catechin present in the plant that requires further isolation and characterization with respect to anti-hypertrophic therapeutics.

The Effectiveness of Tsu-San-Li (St-36) and Tai-Chung (Li-3) Acupoints for Treatment of Acute Liver Damage in Rats

2001 ◽  
Vol 29 (02) ◽  
pp. 221-226 ◽  
Author(s):  
Hsu-Jan Liu ◽  
Sheng-Feng Hsu ◽  
Chang-Chi Hsieh ◽  
Tin-Yun Ho ◽  
Ching-Liang Hsieh ◽  
...  
Keyword(s):  
Carbon Tetrachloride ◽  
Acupuncture Therapy ◽  
Liver Injury ◽  
Acute Liver Injury ◽  
Male Rats ◽  
Acute Liver Damage ◽  
Glutamate Pyruvate Transaminase ◽  
Serum Glutamate Pyruvate Transaminase ◽  
Glutamate Pyruvate ◽  
Serum Glutamate

This study investigated the effects of acupuncture on carbon tetrachloride (CCl4) induced acute liver injury in male rats (n = 36). The experimental groups were injected with CCl4 before, during, or after acupuncture therapy. Acupoints similar to the human Tsu-San-Li (St-36) and Tai-Chung (Li-3) were needled bilaterally. Rats treated with CCl4 had higher levels of serum glutamate-oxalate-transaminase (sGOT) and serum glutamate-pyruvate-transaminase (sGPT). Comparing the experimental groups, biochemical and pathological parameters of liver injury were significantly reduced when rats were acupunctured after, not before, CCl4-induced hepatotoxicity. Acupuncture at the Tsu-Sa-Li and Tai-Chung acupoints cannot prevent acute liver injury but may be effective in treating liver injury induced by carbon tetrachloride in rats.

Hepatoprotective Effect of Phyllanthus in Taiwan on Acute Liver Damage Induced by Carbon Tetrachloride

2006 ◽  
Vol 34 (03) ◽  
pp. 471-482 ◽  
Author(s):  
Chao-Ying Lee ◽  
Wen-Huang Peng ◽  
Hao-Yuan Cheng ◽  
Fei-Na Chen ◽  
Ming-Tsung Lai ◽  
...  
Keyword(s):  
Liver Damage ◽  
Reduced Glutathione ◽  
Protective Effects ◽  
Acute Liver Damage ◽  
Glutamate Pyruvate Transaminase ◽  
Methanolic Extracts ◽  
Antioxidant Agents ◽  
Serum Glutamate Pyruvate Transaminase ◽  
Glutamate Pyruvate ◽  
Serum Glutamate

The effect of oral administration of Phyllanthus methanolic extracts (PME) (i.e. P. acidus, P. emblica, P. myrtifolius, P. multiflorus, P. amarus, P. debilis, P. embergeri, P. hookeri, P. tenellus, P. urinaria L.s. nudicarpus, P. urinaria L.s. urinaria) or gallic acid (GA) on the progression of acute liver damage induced by CCl 4 in rats was examined by morphological and biochemical methods. P. acidus, P. urinaria L.s. urinaria, GA at a dose of 0.5 g/kg, and P. emblica, P. urinaria L.s. nudicarpus at a dose of 1.0 g/kg attenuated CCl 4-induced increase in serum glutamate-oxalate-transaminase (GOT). P. acidus, P. urinaria L.s. nudicarpus, P. urinaria L.s. urinaria, GA at a dose of 0.5 g/kg, and P. emblica, P. amarus, P. hookeri, P. tenellus at a dose of 1.0 g/kg attenuated CCl 4-induced increase in serum glutamate-pyruvate-transaminase (GPT). Concurrently, P. acidus, P. multiflorus, P. embergeri, P. hookeri, P. tenellus and P. urinaria L.s. urinaria elevated the activity of liver reduced glutathione peroxidase (GSH-Px). Since the protective effects of P. acidus, P. emblica, P. myrtifolius, P. embergeri, P. urinaria L.s. nudicarpus, P. urinaria L.s. urinaria and GA correlate with a reduction in liver infiltration and focal necrosis observed using histological methods, these data demonstrate that P. acidus and P. urinaria L.s. urinaria are hepatoprotective and antioxidant agents.

scholarly journals Efek Proteksi Pemberian Ekstrak Daun Jamblang (Syzygium cumini) Terhadap Peningkatan Aktivitas Serum Glutamate Pyruvate Transaminase Tikus (Rattus norvegicus) Jantan Yang Diinduksi Timbal Asetat

2020 ◽  
Vol 1 (2) ◽  
Author(s):  
ADILLA OKTARIZA ZARWIN
Keyword(s):  
Pyridoxal Phosphate ◽  
Glutamate Pyruvate Transaminase ◽  
Group Design ◽  
Post Test ◽  
Serum Glutamate Pyruvate Transaminase ◽  
Glutamate Pyruvate ◽  
Post Hoc ◽  
Per Oral ◽  
Serum Glutamate ◽  
One Way Anova

Timbal (Pb) merupakan suatu logam berat yang bersifat toksik bagi tubuh. Timbal dapat menginduksi pembentukan radikal bebas yang dapat menimbulkan stres oksidatif sehingga berujung pada kematian sel. Kematian sel menstimulasi peningkatan enzim hati seperti SGPT dan SGOT. Tubuh memerlukan antioksidan eksogen untuk menghambat stres oksidatif akibat radikal bebas. Daun jamblang mengandung antioksidan alami seperti flavonoid dan fenolik yang dapat menangkal radikal bebas. Penelitian ini bertujuan untuk mengetahui efek proteksi pemberian ekstrak daun jamblang terhadap peningkatan aktivitas serum glutamate pyruvate transaminase (SGPT) tikus jantan yang diinduksi timbal asetat. Penelitian ini adalah penelitian eksperimental dengan rancangan randomized post test only group design menggunakan 15 ekor tikus jantan yang dibagi atas 3 kelompok yaitu kelompok kontrol negatif (K-) yang diberikan pakan standar, kelompok kontrol positif (K+) yang diberikan pakan standar dan timbal asetat dosis 40 mg/KgBB per oral, kelompok perlakuan 1 (P1) yang diberikan pakan standar serta timbal asetat dosis 40 mg/KgBB dan ekstrak daun jamblang dosis 150 mg/KgBB per oral. Penelitian ini dilakukan selama 28 hari dan di hari ke-29 tikus dimatikan lalu diambil serumnya untuk diperiksa kadar SGPT. Pemeriksaan kadar SGPT menggunakan tes berdasarkan kriteria IFCC tanpa pyridoxal phosphate dengan metode Sample Start. Data dianalisis dengan uji Shapiro Wilk, uji One Way Anova dan uji Post Hoc Tukey’s HSD. Hasil penelitian ini menunjukkan rerata kadar SGPT kelompok kontrol negatif (K-) 46,6 U/L, kelompok kontrol positif (K+) 73,9 U/L, dan kelompok perlakuan 1 (P1) 62,0 U/L. Hasil ini menunjukkan perbedaan bermakna rerata kadar SGPT semua kelompok hewan coba yang dibuktikan dengan nilai p < 0,05. Kesimpulan penelitian ini adalah adanya efek proteksi pemberian ekstrak daun jamblang terhadap peningkatan aktivitas SGPT tikus yang diiinduksi oleh timbal asetat. Kata Kunci: Timbal asetat, ekstrak daun jamblang, SGPT.

Effects of selenium, zinc, insulin and metallothionein on cadmium-induced oxidative stress and metallothionein gene expression levels in diabetic rats

2020 ◽  
Vol 31 (2) ◽  
Author(s):  
Huseyin Gungor ◽  
Haki Kara
Keyword(s):  
Oxidative Stress ◽  
Gene Expression ◽  
Diabetic Rats ◽  
Control Group ◽  
Activity Levels ◽  
Sod Activity ◽  
Expression Levels ◽  
Group 6 ◽  
Group 5 ◽  
Gene Expression Levels

AbstractBackgroundThe aim of this study was to investigate the effects of selenium, zinc, insulin, and metallothionein on oxidative damage and metallothionein (MT) gene expression levels in streptozotocin (STZ)-induced type 1 diabetic rats exposed to Cd.MethodsRats were categorized under eight groups (control, STZ, Cd, STZ + Cd, Group 5, Group 6, Group 7, and STZ + Cd + MT [n:8/group]) were used. After diabetes was induced by STZ (55 mg/kg, i.p.), Cd was administered (1 mg/kg CdCl, orally) for 4 weeks. In cadmium-treated groups selenium (Na2SeO3 1.5 mg/kg, i.p.), zinc (ZnSO4 10 mg/kg via oral gavage), insulin (insulin glargine, 2U/day, s.c.), and MT (1mg/kg, every other 10 days, s.c.) were administered. MT gene expression levels, MDA levels, GPx, SOD, and CAT activity levels were determined in liver and kidney tissues.ResultsMT gene expression and MDA levels increased (p < 0.05) while GPx and SOD activity levels decreased (p < 0.05) in STZ, Cd, and STZ + Cd groups. In Group 5, Group 6, Group 7, and Group 8 groups MT gene expression and MDA levels were decreased while GPx and SOD activity levels were increased (p < 0.05). CAT activity significantly increased (p < 0.05) in STZ + Cd group while there were no significance in other groups (p > 0.05). Compared to the control, Group 5, Group 6, Group 7, and Group 8 groups provided no difference for alanine aminotransferase, aspartate aminotransferase, blood urea nitrogen and creatinine levels (p > 0.05).ConclusionsOur results suggest that Se, insulin, Zn and MT may have protective effects against hepatotoxicity and nephrotoxicity caused by Cd exposure in diabetic rats by reducing oxidative stress and MT gene expression levels.

Evaluation of Anticonvulsant Activity and Toxicity Screening of Semicarbazones Derived from Quinazolinone Scaffold

2019 ◽  
Vol 15 (5) ◽  
pp. 573-581
Author(s):  
Meena K. Yadav ◽  
Laxmi Tripathi ◽  
Diptendu Goswami
Keyword(s):  
Anticonvulsant Activity ◽  
Toxicity Screening ◽  
Protective Index ◽  
Mes Test ◽  
Seizure Models ◽  
Serum Glutamate Pyruvate Transaminase ◽  
Glutamate Pyruvate ◽  
The Given ◽  
Higher Doses ◽  
Serum Glutamate

Background: Epilepsy is one of the most prevalent noncommunicable neurological conditions. More than 10 million people in India are afflicted with epilepsy. Treatment available has many detrimental side effects. Up to one-third of epilepsy patients remain resistance to optimum drug treatment. These facts triggered the further scope and search for newer more effective and less toxic anticonvulsants. Methods: Quinazolinone semicarbazone derivatives showing protection in chemoconvulsant induced seizure models (as reported in our previous study) were further screened in MES and scPTZ induced seizure models. Neurotoxicity was determined; quantification of anticonvulsant activity and toxicity was also done. Finally compounds were screened by liver functional test to ascertain the possible hepatotoxicity in the active compounds. Results: Compounds N-1- (menthone) -N- [3-(4-(substituted)-phenyl) -4-oxo- 3,4-dihydroquinazolin- 2-yl] methyl semicarbazone (3A-d-4, 3B-d-4 and 3C-d-4) showed significant protection in both MES and scPTZ induced seizure model with no neurotoxicity at the given dose. In MES test, compounds showed an ED50 close to that of phenytoin and carbamazepine. They also showed Protective Index (PI) higher as compared to phenytoin and carbamazepine. A high safety profile (HD50/ED50 values) was noted and hypnosis, analgesia, and anesthesia were only observed at higher doses. Conclusion: Compounds showed no significant increase or decrease in the concentration of alkaline phosphatase, Serum Glutamate Oxaloacetate Transaminase (SGOT), Serum Glutamate Pyruvate Transaminase (SGPT), albumin and bilirubin.

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