PEGYLATED CONJUGATED OLIGOMERS FOR TARGETED TWO-PHOTON FLUORESCENCE IMAGING OF CANCER CELLS

2013 ◽  
Vol 01 (03) ◽  
pp. 1340011
Author(s):  
GUAN WANG ◽  
JUNLONG GENG ◽  
XINHAI ZHANG ◽  
GUICHUAN XING ◽  
TZE CHIEN SUM ◽  
...  
Keyword(s):  
Quantum Yield ◽  
Fluorescence Imaging ◽  
Cross Section ◽  
Fluorescence Quantum Yield ◽  
Photon Absorption ◽  
Fluorescence Measurement ◽  
Experimental Conditions ◽  
Two Photon ◽  
Two Photon Fluorescence ◽  
Photon Fluorescence

A water-dispersible PEGylated conjugated oligomer (PEGTVFVBN) is synthesized for two-photon fluorescence imaging applications. PEGTVFVBN could aggregate into nanoparticles of ~ 110 nm size in H 2 O , which show a fluorescence quantum yield of 0.12, 20 times higher than that for its cationic counterpart (TVFVBC). Two-photon fluorescence measurement reveals a maximum two-photon absorption (TPA) cross-section of 263 GM at 780 nm based on molecules for PEGTVFVBN nanoparticles in H 2 O , while the TPA spectra of TVFVBC are barely detectable under the same experimental conditions. The N , N' -diacetylchitobiotic acid conjugated derivative (ChPEGTVFVBN) could also aggregate into nanoparticles of ~ 90 nm sizes, showing a slightly smaller fluorescence quantum yield of 0.08 and a maximum TPA cross-section of 213 GM at 780 nm. ChPEGTVFVBN could be specifically internalized by vimentin over expressing Hela cells through a receptor mediated endocytosis, giving two-photon fluorescence images in a bright and high contrast manner. In addition, both PEGTVFVBN and ChPEGTVFVBN show low cytotoxicity, which is essential for cell imaging applications. This study thus highlights the molecular engineering strategy of PEGylation to construct robust TPA materials for two-photon fluorescence imaging applications.

Elucidating the relationship between superoxide anion levels and lifespan using an enhanced two-photon fluorescence imaging probe

2015 ◽  
Vol 51 (47) ◽  
pp. 9710-9713 ◽  
Author(s):  
Wen Zhang ◽  
Xin Wang ◽  
Ping Li ◽  
Fang Huang ◽  
Hui Wang ◽  
...  
Keyword(s):  
Fluorescence Imaging ◽  
Cross Section ◽  
Absorption Cross Section ◽  
Superoxide Anion ◽  
Absorption Cross ◽  
Fluorescence Response ◽  
Two Photon ◽  
Two Photon Fluorescence ◽  
The Relationship ◽  
Photon Fluorescence

We report a new reversible fluorescent two-photon (TP) probe (PY-CA) with high TP absorption cross section and pH-independent fluorescence response, which allow monitoring of O2˙− fluxes dynamically, selectively and sensitively.

INFLUENCES OF AGGREGATION ON THE TWO-PHOTON FLUORESCENCE PROCESS

2013 ◽  
Vol 22 (01) ◽  
pp. 1350011 ◽  
Author(s):  
ZHIQIANG ZHOU ◽  
CHANGGUI LU ◽  
YIPING CUI
Keyword(s):  
Measurement Error ◽  
Cross Section ◽  
Correction Method ◽  
Photon Absorption ◽  
Two Photon Absorption ◽  
Two Photon ◽  
Large Measurement ◽  
Two Photon Fluorescence ◽  
Photon Fluorescence ◽  
Large Measurement Error

Rhodamine B (RhB) is most often used as the reference in the two-photon induced fluorescence (TPIF) method to measure the two-photon absorption (TPA) cross-section. In this work, we investigate the influences of dimer formation, energy transfer and self-absorption on the two-photon fluorescence process. Those three effects will result in a large measurement error of the TPA cross-section. We thus provide a concentration gradient correction method by considering the above three processes to eliminate the measurement error.

Nonlinear optical property and ultrafast response of a two-photon photopolymerization initiator based on dibenzothiophene

2017 ◽  
Vol 26 (04) ◽  
pp. 1750048 ◽  
Author(s):  
Yaochuan Wang ◽  
Dajun Liu ◽  
Yizhuo Wang ◽  
Guiqiu Wang ◽  
Xiaoqiang Yu
Keyword(s):  
Cross Section ◽  
Fluorescence Emission ◽  
Photon Absorption ◽  
Time Resolved ◽  
Pump Probe ◽  
Two Photon Absorption ◽  
Two Photon ◽  
Uv Visible ◽  
Two Photon Fluorescence ◽  
Photon Fluorescence

A dibenzothiophene-based two-photon photopolymerization (TPP) initiator named E,E-1,4-bis[3-vinyl-dibenzothiophene]-2,5-dimethoxybenzene (VBPMOB) with intense two-photon fluorescence (TPF) was investigated by UV–visible, fluorescence, pump–probe and time-resolved fluorescence spectroscopies. The maximum two-photon absorption (TPA) cross-section of VBPMOB in DMF was determined to be 274[Formula: see text]GM. In addition, the TPA cross-section at 800[Formula: see text]nm was 230[Formula: see text]GM, which is the most commonly used wavelength for TPP. The ultrafast spectra result indicated that after excitation, the VBPMOB molecule will first undergo a fast internal conversion process to form an intramolecular charge transfer (ICT) state with a certain width followed by relaxation of the ICT state with fluorescence emission.

Lysosomal targeted NIR photosensitizer for photodynamic therapy and two-photon fluorescent imaging

2021 ◽  
Author(s):  
Ruiyuan Liu ◽  
Yuping Zhou ◽  
Di Zhang ◽  
Genghan He ◽  
Chuang Liu ◽  
...  
Keyword(s):  
Photodynamic Therapy ◽  
Fluorescence Imaging ◽  
Near Infrared ◽  
Fluorescent Imaging ◽  
High Fidelity ◽  
Design And Synthesis ◽  
Two Photon ◽  
Two Photon Fluorescence ◽  
Photon Fluorescence

Design and synthesis of near-infrared (NIR) emissive fluorophore for imaging of organelle and photodynamic therapy has received enormous attention. Hence, NIR emissive fluorophore of high-fidelity lysosome targeting, two-photon fluorescence imaging,...

Interactions of picosecond laser pulses with organic molecules I. Two-photon fluorescence quenching and singlet states excitation in Rhodamine dyes

1972 ◽  
Vol 328 (1572) ◽  
pp. 97-121 ◽  
Keyword(s):  
Rhodamine 6G ◽  
Second Harmonic ◽  
Random Phase ◽  
Stimulated Emission ◽  
Photon Absorption ◽  
Picosecond Pulses ◽  
Two Photon ◽  
Singlet States ◽  
Two Photon Fluorescence ◽  
Photon Fluorescence

Intensity dependent quenching and reversal of the two-photon fluorescence patterns in Rhodamine 6G and DPA, of picosecond pulses from a mode-locked ruby laser have been investigated by measurements of the two-photon fluorescence efficiencies of these dyes. While for Rhodamine 6G there was a marked departure from the square law dependence at high laser intensities, the experimental curve for DPA showed no evidence of quenching. When excited by a mode-locked neodymium: glass laser Rhodamine 6G fluorescence was not quenched at fluxes as high as 5 x 10 30 photons cm -2 s -1 but in Rhodamine B quenching appeared at a laser flux of 3 x 10 27 photons cm -2 s -1 . These quenching results and measurements of the absorption of pulses by Rhodamine 6G, previously excited by second harmonic pulses, are explained by the effects of single photon absorption and stimulated emission from the S 1 and S 2 excited singlet states. A square pulse approximation has been employed to solve the general rate equations and the fitting of the calculated curves to the experimental results gave values for the stimulated emission and absorption cross-sections of the S 1 and S 2 states of Rhodamine 6G and for the relaxation time (~ 2 ps) between the vibrational manifolds of these excited states. Taking into account random phase and amplitude fluctuations of the picosecond pulses, time and space averaged two-photon fluorescence profiles, using these values of the dye parameters, showed quenching and reversal of the patterns for the laser pulse intensities at which these effects were experimentally observed. The possibilities of frequency tunable pulses, of transform-limited durations, from mode-locked dye lasers employed with an electro-optical streak camera of time-resolution equal to that of the pulse durations (~ 2 ps) for time-resolved excited state molecular spectroscopy are briefly considered.

Enhanced Two-Photon Fluorescence Imaging and Therapy of Cancer Cells via Gold@Bridged Silsesquioxane Nanoparticles

Small ◽  
2014 ◽  
Vol 11 (3) ◽  
pp. 295-299 ◽  
Author(s):  
Jonas Croissant ◽  
Marie Maynadier ◽  
Olivier Mongin ◽  
Vincent Hugues ◽  
Mireille Blanchard-Desce ◽  
...  
Keyword(s):  
Cancer Cells ◽  
Fluorescence Imaging ◽  
Two Photon ◽  
Bridged Silsesquioxane ◽  
Two Photon Fluorescence ◽  
Photon Fluorescence
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