Prussian blue-functionalised graphene in the amperometric detection of peroxide and hydrazine

The electrochemical detection of hydrogen peroxide, H 2 O 2, and hydrazine, N 2 H 4, is of considerable interest because of the serious health risks associated with these compounds. The unique zeolite structure of Prussian blue, Fe 4[ Fe ( CN )6]3, endows it with excellent catalytic activity towards these small molecules, but its immobilisation on suitable support materials is limited by its solubility at neutral and basic pH values. Here we report an electroanalytical sensor for peroxide and hydrazine based on composites of Prussian blue and graphene nano-sheets (GNSs). The latter are fabricated using the liquid-phase exfoliation of graphite. When immobilised on graphene, Prussian blue is shown to exhibit higher sensitivity towards these analytes than a number of commercially available graphitic supports, including edge-plane pyrolytic graphite (EPPG) electrodes. The incorporation of graphene into electode systems could lead to great advances in sensor technology for these and many other species of interest.

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A Label and Probe-Free Zika Virus Immunosensor Prussian Blue@carbon Nanotube-Based for Amperometric Detection of the NS2B Protein

Biosensors ◽

10.3390/bios11050157 ◽

2021 ◽

Vol 11 (5) ◽

pp. 157

Author(s):

Bárbara V. M. Silva ◽

Marli T. Cordeiro ◽

Marco A. B. Rodrigues ◽

Ernesto T. A. Marques ◽

Rosa F. Dutra

Keyword(s):

Carbon Nanotube ◽

Prussian Blue ◽

Zika Virus ◽

Point Of Care ◽

Amperometric Detection ◽

Cross Reactivity ◽

Structural Protein ◽

Serum Samples ◽

Polypyrrole Composite ◽

Congenital Disabilities

Zika virus (ZIKV) is a mosquito-borne infection, predominant in tropical and subtropical regions causing international concern due to the ZIKV disease having been associated with congenital disabilities, especially microcephaly and other congenital abnormalities in the fetus and newborns. Development of strategies that minimize the devastating impact by monitoring and preventing ZIKV transmission through sexual intercourse, especially in pregnant women, since no vaccine is yet available for the prevention or treatment, is critically important. ZIKV infection is generally asymptomatic and cross-reactivity with dengue virus (DENV) is a global concern. An innovative screen-printed electrode (SPE) was developed for amperometric detection of the non-structural protein (NS2B) of ZIKV by exploring the intrinsic redox catalytic activity of Prussian blue (PB), incorporated into a carbon nanotube–polypyrrole composite. Thus, this immunosensor has the advantage of electrochemical detection without adding any redox-probe solution (probe-less detection), allowing a point-of-care diagnosis. It was responsive to serum samples of only ZIKV positive patients and non-responsive to negative ZIKV patients, even if the sample was DENV positive, indicating a possible differential diagnosis between them by NS2B. All samples used here were confirmed by CDC protocols, and immunosensor responses were also checked in the supernatant of C6/36 and in Vero cell cultures infected with ZIKV.

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Amperometric detection of catecholamines with liquid chromatography at a novelly constructed prussian blue chemically modified electrode

Talanta ◽

10.1016/0039-9140(92)80026-a ◽

1992 ◽

Vol 39 (3) ◽

pp. 235-242 ◽

Cited By ~ 8

Author(s):

Jianxun Zhou ◽

Erkang Wang

Keyword(s):

Liquid Chromatography ◽

Prussian Blue ◽

Modified Electrode ◽

Amperometric Detection ◽

Chemically Modified Electrode ◽

Chemically Modified

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Amperometric Detection of H2O2 Using Gold Electrodes Modified with Starburst PAMAM Dendrimers and Prussian Blue

Electroanalysis ◽

10.1002/elan.200603619 ◽

2006 ◽

Vol 18 (21) ◽

pp. 2092-2098 ◽

Cited By ~ 9

Author(s):

Erika Bustos Bustos ◽

Thomas W. Chapman ◽

F. Rodríguez-Valadez ◽

Luis A. Godínez

Keyword(s):

Prussian Blue ◽

Amperometric Detection ◽

Pamam Dendrimers ◽

Gold Electrodes

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Rhodium-Prussian Blue modified carbon paste electrode (Rh-PBMCPE) for amperometric detection of hydrogen peroxide

Journal of the Brazilian Chemical Society ◽

10.1590/s0103-50532003000400010 ◽

2003 ◽

Vol 14 (4) ◽

pp. 551-555 ◽

Cited By ~ 12

Author(s):

Viviane Midori Ivama ◽

Silvia H. P. Serrano

Keyword(s):

Hydrogen Peroxide ◽

Prussian Blue ◽

Carbon Paste Electrode ◽

Amperometric Detection ◽

Modified Carbon Paste Electrode ◽

Carbon Paste ◽

Paste Electrode

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Amperometric detection of glucose and H2O2 using peroxide selective electrode based on carboxymethylcellulose/polypyrrole and Prussian Blue nanocomposite

Materials Today Communications ◽

10.1016/j.mtcomm.2020.101839 ◽

2020 ◽

pp. 101839

Author(s):

Sinan Uzunçar ◽

Nizamettin Özdoğan ◽

Metin Ak

Keyword(s):

Prussian Blue ◽

Amperometric Detection ◽

Selective Electrode

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ELECTROCHEMICAL DNA SENSOR TECHNOLOGY FOR MONITORING OF DRUG–DNA INTERACTIONS

NANO ◽

10.1142/s1793292008001064 ◽

2008 ◽

Vol 03 (04) ◽

pp. 229-232 ◽

Cited By ~ 5

Author(s):

A. ERDEM ◽

H. KARADENIZ ◽

A. CALISKAN ◽

A. VASEASHTA

Keyword(s):

Drug Delivery ◽

Small Molecules ◽

Anticancer Drugs ◽

Point Of Care ◽

Sensor Technology ◽

Electrochemical Biosensors ◽

Dna Sensor ◽

Dna Interactions ◽

Electrochemical Dna Sensor

The objective of this investigation is to understand the nature and dynamics of binding small molecules to bio-macromolecules using electrochemical methods. The investigation pertaining to the design of site- and conformation-specific reagents provides a rationale for new studies of drug delivery design. Some anticancer drugs and DNA interactions have been undertaken by using a variety of techniques. Determination of interaction between DNA and DNA-targeted molecules would be valuable in the design of molecule-specific electrochemical biosensors for applications in diagnostics, development of drugs for chemotherapy, and as a biotechnological tool for DNA-based point-of-care diagnosis.

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Morphological and Electrical Properties of Silicon Dioxide-Based Interdigitated Electrode Arrays

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.1109.253 ◽

2015 ◽

Vol 1109 ◽

pp. 253-256 ◽

Cited By ~ 1

Author(s):

M.A. Farehanim ◽

U. Hashim ◽

Norhayati Soin ◽

A.H. Azman ◽

S. Norhafiezah ◽

...

Keyword(s):

Electrical Properties ◽

Silicon Dioxide ◽

Ph Value ◽

Ph Measurement ◽

Electrode Arrays ◽

Interdigitated Electrode ◽

Buffer Solutions ◽

Ph Values ◽

Ph Buffer ◽

Higher Sensitivity

The electrical performances of silicon dioxide-based Interdigitated electrodes (IDEs) as biosensor were developed. The IDEs was made up by two individually addressable Interdigitated comb-like finger structure have frequently been suggested as a biosensor which promises higher sensitivity compared to conventional parallel electrodes. The purpose of this paper was to investigate the capacitance test and impedance test to taken with various pH solution to observe the response of the sensor with different pH values. Purchased pH buffer solutions which varied from pH2 to pH10 are dropped on the microelectrode and the effect on it is investigated for the application in pH measurement. This research has proven that increase in pH value from acidic to alkaline is proportional with capacitance. The measured values of capacitance with respect to each pH concentrations applied during the measurements were repeatable and reproducible.

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