Automated Printing of Electrochemical Immunoassay Microarrays: Studies of Conditions Affecting Alkaline Phosphatase Enzyme Label Activity

2019 ◽  
Vol 28 (21) ◽  
pp. 19-33
Author(s):  
Caitlin R. Williams ◽  
Ingrid Fritsch
Keyword(s):  
Alkaline Phosphatase ◽  
Electrochemical Immunoassay ◽  
Enzyme Label ◽  
Phosphatase Enzyme

Electrochemical immunoassay: Simple kinetic detection of alkaline phosphatase enzyme labels in limited and excess reagent systems

1994 ◽  
Vol 6 (7) ◽  
pp. 561-566 ◽  
Author(s):  
Paul H. Treloar ◽  
Asa'ah T. Nkohkwo ◽  
John W. Kane ◽  
Don Barber ◽  
Pankaj M. Vadgama
Keyword(s):  
Alkaline Phosphatase ◽  
Electrochemical Immunoassay ◽  
Excess Reagent ◽  
Enzyme Labels ◽  
Phosphatase Enzyme

Alkaline phosphatase enzyme-induced biomineralization of chitosan scaffolds with enhanced osteogenesis for bone tissue engineering

2019 ◽  
Vol 371 ◽  
pp. 618-630 ◽  
Author(s):  
Na Li ◽  
Lin Zhou ◽  
Weilong Xie ◽  
Deyi Zeng ◽  
Daqian Cai ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Alkaline Phosphatase ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Chitosan Scaffolds ◽  
Phosphatase Enzyme

Acute Hepatopathy Associated With Mitotane Administration in a Dog

2006 ◽  
Vol 42 (4) ◽  
pp. 298-301 ◽  
Author(s):  
Craig B. Webb ◽  
David C. Twedt
Keyword(s):  
Alkaline Phosphatase ◽  
Enzyme Activity ◽  
Supportive Care ◽  
Prothrombin Time ◽  
Liver Enzyme ◽  
Hepatic Failure ◽  
Enzyme Activities ◽  
Total Bilirubin ◽  
Persistent Elevation ◽  
Phosphatase Enzyme

An adult dog with a persistent elevation in alkaline phosphatase enzyme activity was started on mitotane for suspected hyperadrenocorticism. One month later, the dog was presented for intermittent anorexia and acute icterus. The dog’s liver enzyme activities and total bilirubin were markedly elevated, prothrombin time was prolonged, and blood urea nitrogen and glucose were low. Histopathology revealed marked, centrilobular, hepatocellular loss. After discontinuation of the mitotane, the dog recovered with supportive care and was normal 3 months later, which was consistent with mitotane-associated hepatic failure.

Effect of incubation period on measurement of alkaline phosphatase enzyme activity at 37°C

2017 ◽  
Vol 6 (5) ◽  
pp. 1 ◽  
Author(s):  
Nilam Karbhari ◽  
Dipti Karbhari ◽  
Manmeet Kochar ◽  
Shailesh Patel
Keyword(s):  
Alkaline Phosphatase ◽  
Enzyme Activity ◽  
Incubation Period ◽  
Phosphatase Enzyme

Polyphenolic Compounds from Flowers of Hibiscus rosa-sinensis Linn. and their Inhibitory Effect on Alkaline Phosphatase Enzyme Activity in vitro

2011 ◽  
Vol 66 ◽  
pp. 0453 ◽  
Author(s):  
J. Y. Salib ◽  
E. N. Daniel ◽  
M. S. Hifnawy ◽  
S. M. Azzam ◽  
I. B. Shaheed ◽  
...  
Keyword(s):  
Alkaline Phosphatase ◽  
Enzyme Activity ◽  
Inhibitory Effect ◽  
Polyphenolic Compounds ◽  
Hibiscus Rosa Sinensis ◽  
Phosphatase Enzyme

scholarly journals Assessment of the microbiological activity in agricultural and urban soils

2014 ◽  
Vol 65 (4) ◽  
pp. 156-160 ◽  
Author(s):  
DOROTA DEC
Keyword(s):  
Alkaline Phosphatase ◽  
Acid Phosphatase ◽  
Dehydrogenase Activity ◽  
Urban Soil ◽  
Urban Soils ◽  
Agricultural Soils ◽  
C:N Ratio ◽  
Soil Samples ◽  
Microbiological Activity ◽  
Phosphatase Enzyme

Abstract The aim of this study was to evaluate the enzymatic soil and the number of selected microorganisms in urban soil, which are located in the lane of the reconstructed road and compare it with a soil cultivated for agricultural purposes. The conducted analysis showed significant differences between the results of the soil taken from the roadway and the soil cultivated from agricultural purposes. The C:N ratio in soils of the roadway (from 24 to 31) indicated that they were degraded and heavily degraded soils. Urban soils had a neutral pH. The activity of dehydrogenase (1.93–6.95 μg TPF g−1·h−1), acid phosphatase (2.42–4.92 mM pNP·g−1·h−1) and alkaline phosphatase (2.34–4.80 mM pNP·g−1·h−1) in urban soils were low. In agricultural soils the acid phosphatase enzyme levels ranged 6.32–8.04 mM pNP·g−1·h−1, and alkaline phosphatase were 7.26–9.16 mM pNP·g−1·h−1. In urban soil samples collected along the roadway, a significant correlation between potassium and dehydrogenase activity, and between the C:N ratio and the activity of acid phosphatase was found.

Competitive heterogeneous enzyme immunoassay for theophylline by flow-injection analysis with electrochemical detection of p-aminophenol

1990 ◽  
Vol 36 (4) ◽  
pp. 662-665 ◽  
Author(s):  
E P Gil ◽  
H T Tang ◽  
H B Halsall ◽  
W R Heineman ◽  
A S Misiego
Keyword(s):  
Alkaline Phosphatase ◽  
Detection Limit ◽  
Electrochemical Detection ◽  
Flow Injection ◽  
Flow Injection Analysis ◽  
General Procedure ◽  
Amperometric Detection ◽  
Enzyme Label ◽  
Commercial Kit

Abstract A competitive enzyme-linked immunoabsorbent assay based on the flow-injection amperometric detection of p-aminophenol has been investigated with use of the materials and general procedure of a commercial kit for the determination of theophylline in human serum. The antibody is immobilized on glass beads, and the enzyme label is alkaline phosphatase (EC 3.1.3.1). The high currents generated during the electrochemical detection allowed a rapid (35 min) and simple determination of theophylline throughout its therapeutic range (10-20 mg/L) and also in the subtherapeutic range (detection limit of about 80 micrograms/L).

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