scholarly journals Development of an Effective and Economic Biosensor for Diabetic Blood Monitoring Based on MWCNTs, Artificial Redox Mediator Ferrocene, Nafion Polymer and a Local Extracted and Purified Glucose Oxidase Enzyme from Penicillium Notatum F-158 Fungus

2021 ◽  
Author(s):  
Hanaa El-Desoky ◽  
Ashraf Koleeb ◽  
Roqaya Bassuiny ◽  
Tarek Mohamed
Keyword(s):  
Glucose Oxidase ◽  
Redox Mediator ◽  
Enzymatic Oxidation ◽  
Layer By Layer ◽  
Plasma Samples ◽  
Penicillium Notatum ◽  
Glucose Determination ◽  
Fruit Samples ◽  
Oxidase Enzyme ◽  
Orange Fruit

Abstract Enhancement of the properties of glucose oxidase (GOx) enzyme is still receiving attention due to its extensive applications. Eight different fungal strains were isolated from soil and orange fruit samples for inexpensive GOx production. Penicillium notatum F-158 (P. notatum) strain produced a remarkable amount of GOx. Its culture condition was optimized for optimum GOx production. GOx was purified and its activity, stability and kinetic parameters were studied. An effective biosensor {GCE/[MWCNTs–Fc–GOx(FAD)–NF]} based on layer by layer immbolization of MWCNTs, aritificial ferrocene (Fc) redox mediator, extracted P. notatum GOx enzyme and nafion polymer (NF) on glassy carbon electrode (GCE) surface was developed for glucose determination. Fc acts as an electron relay between enzyme and MWCNTs/GCE. The synergy of MWCNTs and Fc enhances the electrocatalytic action of Fc to the enzymatic oxidation of glucose. Low potential (+0.3V) of Fc applied in amperometric measurements avoids interference of the main electroactive substances present in the real plasma samples. This biosensor showed broad linear ranges {2.80×10-4 to 14.99×10-3 M} and low detection limit (8.68×10-6 M) for glucose determination. The achieved glucose concentrations in six plasma samples are consistent with normal values in human blood indicating that such biosensor could be used clinically.

Layer-by-Layer Self-Assembly of Glucose Oxidase with a Poly(allylamine)ferrocene Redox Mediator

Langmuir ◽  
1997 ◽  
Vol 13 (10) ◽  
pp. 2708-2716 ◽  
Author(s):  
Jose Hodak ◽  
Roberto Etchenique ◽  
Ernesto J. Calvo ◽  
Kavita Singhal ◽  
Philip N. Bartlett
Keyword(s):  
Glucose Oxidase ◽  
Self Assembly ◽  
Redox Mediator ◽  
Layer By Layer

scholarly journals Carbon nanofiber modified with osmium based redox polymer for glucose sensing

2017 ◽  
Vol 7 (4) ◽  
pp. 181-191 ◽  
Author(s):  
Amos Mugweru ◽  
Reaz Mahmud ◽  
Kartik Ghosh ◽  
Adam Wanekaya
Keyword(s):  
Glucose Oxidase ◽  
Carbon Nanofibers ◽  
Carbon Nanofiber ◽  
Energy Dispersive Spectrometer ◽  
High Sensitivity ◽  
Glucose Sensing ◽  
Redox Polymer ◽  
Glucose Determination ◽  
Km Value ◽  
Oxidase Enzyme

Electrochemical detection of glucose was performed on carbon nanofibers containing an osmium based redox polymer and using glucose oxidase enzyme. Redox polymer assembled on the nanofibers provided a more stable support that preserved enzyme activity and promoted the electrical communication to the glassy carbon electrode. The morphologies, structures, and electrochemical behavior of the redox polymer modified nanofibers were characterized by scanning electron microscope, energy dispersive spectrometer and voltammetry. The glucose oxidase showed excellent communication with redox polymer as observed with the increased activity toward glucose. Both cyclic voltammetry and amperometry showed a linear response with glucose concentration.  The linear range for glucose determination was from 1 to 12 mM with a relatively high sensitivity of 0.20±0.01 μA mM−1 for glucose oxidase in carbon nanofibers and 0.10±0.01 μA mM−1 without carbon nanofibers. The apparent Michaelis–Menten constant (Km) for glucose oxidase with carbon nanofibers was 0.99 mM. On the other hand, the Km value for the glucose oxidase without the nanofibers was 4.90 mM.

scholarly journals Purification and Characterization of the Glucose Oxidase from Penicillium notatum

2018 ◽  
Vol 9 (01) ◽  
Author(s):  
Baydaa A. Hassan ◽  
Mohammed A. Jebor ◽  
Zahra M. Ali
Keyword(s):  
Molecular Weight ◽  
Glucose Oxidase ◽  
Specific Activity ◽  
Gel Filtration ◽  
Maximum Activity ◽  
Purification And Characterization ◽  
Penicillium Notatum ◽  
Oxidase Enzyme ◽  
Filtration Technique

This study aims to purification and characterization of the glucose oxidase enzyme from Penicillium notatum, the enzyme was purified by ammonium sulfate precipitation (60%), dialysis and gel filtration chromatography using sephadex G-200, A trial for the purification of glucose oxidase using gel filtration technique resulted in one type of glucose oxidase with specific activity of (62.382 U/mg) with (7.385 folds) purification. the purified glucose oxidase had a maximum activity at pH = 5.5, 45 °C, glucose oxidase was stable with pH values ranging between (5 – 6) and the enzyme was maintained the activity when it incubated into (25 -35) °C for 15 minutes, analyses of the glucose oxidase for molecular weight was carried out by PAGE and SDS-PAGE electrophoresis, which revealed 78 KDa, also molecular weight of the glucose oxidase was achieved by gel filtration technique and was found 87 KDa this means that enzyme consisting of only one subunit, the Km and Vmax value of glucoamylase (B) were (19.6 mM, 7.5 mM/min ) respectively using different concentration of glucose.

The Structure of Layer-by-Layer Self-Assembled Glucose Oxidase and Os(Bpy)2ClPyCH2NH−Poly(allylamine) Multilayers:  Ellipsometric and Quartz Crystal Microbalance Studies

Langmuir ◽  
2002 ◽  
Vol 18 (10) ◽  
pp. 4020-4029 ◽  
Author(s):  
Erica S. Forzani ◽  
Marcelo Otero ◽  
Manuel A. Pérez ◽  
Manuel López Teijelo ◽  
Ernesto J. Calvo
Keyword(s):  
Glucose Oxidase ◽  
Quartz Crystal Microbalance ◽  
Quartz Crystal ◽  
Layer By Layer ◽  
Self Assembled

Immobilization of glucose oxidase enzyme (GOD) in large pore ordered mesoporous cage-like FDU-1 silica

2011 ◽  
Vol 70 (3-4) ◽  
pp. 149-153 ◽  
Author(s):  
L.C. Cides da Silva ◽  
C.M.C. Infante ◽  
A.W.O. Lima ◽  
I.C. Cosentino ◽  
M.C.A. Fantini ◽  
...  
Keyword(s):  
Glucose Oxidase ◽  
Large Pore ◽  
Ordered Mesoporous ◽  
Oxidase Enzyme

scholarly journals Performance of Three Enzymic Methods for Filter Paper Glucose Determination

1984 ◽  
Vol 21 (5) ◽  
pp. 411-416 ◽  
Author(s):  
J M Burrin ◽  
C P Price
Keyword(s):  
Glucose Oxidase ◽  
Filter Paper ◽  
Time Course ◽  
Glucose Dehydrogenase ◽  
Home Monitoring ◽  
Diabetic Patients ◽  
Trichloracetic Acid ◽  
Elution Time ◽  
Glucose Determination ◽  
Blood Spots

Collection of blood spots on filter paper offers a practical alternative for home monitoring of diabetic patients. We have compared the merits of three protein precipitants, trichloracetic acid (TCA), perchloric acid (PCA) and sulphosalicylic acid (SSA) for the elution of glucose from the filter paper, and their subsequent effects on three enzymic methods, glucose dehydrogenase (GDH), hexokinase (HK), and glucose oxidase (GOD) for the determination of glucose using a microcentrifugal analyser. The combination of TCA elutant with the GDH method was superior with respect to time course of reaction and elution time from the filter paper, and was chosen for routine use. Within- and between-batch precision for this method was 2·7% and 3·2% respectively at normal glucose concentrations. Recovery of glucose added to whole blood was 110 ± 5%. Comparison with an automated glucose oxidase method for plasma glucose gave a slope of 1·1, intercept of −0·7 and a correlation coefficient of 0·9 ( n = 64). We conclude that the combination of TCA and glucose dehydrogenase provides a robust, precise and accurate method for the quantitation of glucose in filter-paper blood spots. The procedure offers increased sensitivity and better precision than GOD methods. The use of TCA as elutant gives a faster elution time and has the least effect on any of the enzymic methods.

Layer by layer self-assembly immobilization of glucose oxidase onto chitosan-graft-polyaniline polymers

2006 ◽  
Vol 41 (10) ◽  
pp. 3147-3149 ◽  
Author(s):  
Xin Hua Xu ◽  
Guang Lei Ren ◽  
Juan Cheng ◽  
Qiang Liu ◽  
Dong Guang Li ◽  
...  
Keyword(s):  
Glucose Oxidase ◽  
Self Assembly ◽  
Layer By Layer

The Peroxidase-Glucose Oxidase Enzyme System

1989 ◽  
pp. 31-32 ◽  
Author(s):  
ELISA WOOLRIDGE ◽  
SANDRA L TURCHI ◽  
JOHN R EDWARDS
Keyword(s):  
Glucose Oxidase ◽  
Enzyme System ◽  
Oxidase Enzyme
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