Tissue distribution of immunoreactive mouse extracellular superoxide dismutase

1998 ◽  
Vol 275 (3) ◽  
pp. C840-C847 ◽  
Author(s):  
Tomomi Ookawara ◽  
Nobuo Imazeki ◽  
Osamu Matsubara ◽  
Takako Kizaki ◽  
Shuji Oh-Ishi ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Superoxide Dismutase ◽  
Adrenal Gland ◽  
Sandwich Elisa ◽  
Brown Fat ◽  
Extracellular Superoxide Dismutase ◽  
Physiological Importance ◽  
Vascular Walls ◽  
Mouse Tissues ◽  
White Fat

Protein content and mRNA expression of extracellular superoxide dismutase (EC-SOD) were investigated in 16 mouse tissues. We developed a double-antibody sandwich ELISA using the affinity-purified IgG against native mouse EC-SOD. EC-SOD could be detected in all of the tissues examined (lung, kidney, testis, brown fat, liver, adrenal gland, pancreas, colon, white fat, thymus, stomach, spleen, heart, skeletal muscle, ileum, and brain, in decreasing order of content measured as μg/g wet tissue). Lung showed a markedly higher value of EC-SOD than other tissues. Interestingly, white fat had a high content of EC-SOD in terms of micrograms per milligram protein, which corresponded to that of lung. Kidney showed the strongest expression of EC-SOD mRNA. Relatively strong expression of the mRNA was observed in lung, white fat, adrenal gland, brown fat, and testis. Heart and brain showed only weak signals, and no such expression could be detected in either digestive organs or skeletal muscle. Immunohistochemically, EC-SOD was localized mainly to connective tissues and vascular walls in the tissues examined. Deep staining in the cytosol was observed in the cortical tubular cells of kidney. These results suggest that EC-SOD is distributed systemically in mice and that the physiological importance of this enzyme may be a compensatory adaptation to oxidative stress, particularly in lung and kidney.

scholarly journals Acute exercise increases expression of extracellular superoxide dismutase in skeletal muscle and the aorta

Redox Report ◽  
2008 ◽  
Vol 13 (5) ◽  
pp. 213-216 ◽  
Author(s):  
Yoshiaki Hitomi ◽  
Sumiko Watanabe ◽  
Takako Kizaki ◽  
Takuya Sakurai ◽  
Tohru Takemasa ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Superoxide Dismutase ◽  
Acute Exercise ◽  
Extracellular Superoxide Dismutase

scholarly journals Dietary l-Arginine Supplementation Reduces White Fat Gain and Enhances Skeletal Muscle and Brown Fat Masses in Diet-Induced Obese Rats

2008 ◽  
Vol 139 (2) ◽  
pp. 230-237 ◽  
Author(s):  
Wenjuan Jobgen ◽  
Cynthia J. Meininger ◽  
Scott C. Jobgen ◽  
Peng Li ◽  
Mi-Jeong Lee ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Brown Fat ◽  
Obese Rats ◽  
White Fat ◽  
Arginine Supplementation

scholarly journals Extracellular superoxide dismutase and other superoxide dismutase isoenzymes in tissues from nine mammalian species

1984 ◽  
Vol 222 (3) ◽  
pp. 649-655 ◽  
Author(s):  
S L Marklund
Keyword(s):  
Skeletal Muscle ◽  
Superoxide Dismutase ◽  
Tissue Distribution ◽  
Mammalian Species ◽  
Superoxide Dismutase Activity ◽  
Rat Tissues ◽  
Extracellular Superoxide Dismutase ◽  
Liver And Kidney ◽  
Cuzn Superoxide Dismutase

The contents of extracellular superoxide dismutase, CuZn superoxide dismutase and Mn superoxide dismutase were determined in tissues from nine mammalian species. The pattern of CuZn superoxide dismutase distribution was similar in all species, with high activity in metabolically active organs such as liver and kidney and low activity in, for example, skeletal muscle. Mn superoxide dismutase activity was high in organs with high respiration, such as liver, kidney, and myocardium. Overall the Mn superoxide dismutase activity in organs was almost as high as the CuZn superoxide dismutase activity. The content of extracellular superoxide dismutase was, almost without exception, lower than the content of the other isoenzymes. The pattern of tissue distribution was distinctly different from those of CuZn superoxide dismutase and Mn superoxide dismutase. The tissue distribution of extracellular superoxide dismutase differed among species, but in general there was much in lungs and kidneys and little in skeletal muscle. In man, pig, sheep, cow, rabbit and mouse the overall tissue extracellular superoxide dismutase activities were similar to each other, whereas dog, cat and rat tissues contained distinctly less. There was no general correlation between the tissue extracellular superoxide dismutase activity of any of the various species and the variable plasma activity. The ratio between the plasma and the overall tissue activities was high, for some species over unity, providing further evidence for the notion that one role of extracellular superoxide dismutase is as a plasma protein.

scholarly journals Identification of inducible brown adipocyte progenitors residing in skeletal muscle and white fat

2010 ◽  
Vol 108 (1) ◽  
pp. 143-148 ◽  
Author(s):  
Tim J. Schulz ◽  
Tian Lian Huang ◽  
Thien T. Tran ◽  
Hongbin Zhang ◽  
Kristy L. Townsend ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Adipose Tissue ◽  
Cell Fate ◽  
Brown Fat ◽  
Brown Adipocyte ◽  
Molecular Characteristics ◽  
Fat Cell ◽  
Interscapular Brown Adipose Tissue ◽  
Brown Adipose ◽  
White Fat

Brown fat is specialized for energy expenditure and has therefore been proposed to function as a defense against obesity. Despite recent advances in delineating the transcriptional regulation of brown adipocyte differentiation, cellular lineage specification and developmental cues specifying brown-fat cell fate remain poorly understood. In this study, we identify and isolate a subpopulation of adipogenic progenitors (Sca-1+/CD45−/Mac1−; referred to as Sca-1+ progenitor cells, ScaPCs) residing in murine brown fat, white fat, and skeletal muscle. ScaPCs derived from different tissues possess unique molecular expression signatures and adipogenic capacities. Importantly, although the ScaPCs from interscapular brown adipose tissue (BAT) are constitutively committed brown-fat progenitors, Sca-1+ cells from skeletal muscle and subcutaneous white fat are highly inducible to differentiate into brown-like adipocytes upon stimulation with bone morphogenetic protein 7 (BMP7). Consistent with these findings, human preadipocytes isolated from subcutaneous white fat also exhibit the greatest inducible capacity to become brown adipocytes compared with cells isolated from mesenteric or omental white fat. When muscle-resident ScaPCs are re-engrafted into skeletal muscle of syngeneic mice, BMP7-treated ScaPCs efficiently develop into adipose tissue with brown fat-specific characteristics. Importantly, ScaPCs from obesity-resistant mice exhibit markedly higher thermogenic capacity compared with cells isolated from obesity-prone mice. These data establish the molecular characteristics of tissue-resident adipose progenitors and demonstrate a dynamic interplay between these progenitors and inductive signals that act in concert to specify brown adipocyte development.

Inhibition of iNOS attenuates skeletal muscle reperfusion injury in extracellular superoxide dismutase knockout mice

Microsurgery ◽  
2005 ◽  
Vol 25 (8) ◽  
pp. 606-613 ◽  
Author(s):  
Jong Woong Park ◽  
Wen-Ning Qi ◽  
John Q. Liu ◽  
James R. Urbaniak ◽  
Rodney J. Folz ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Superoxide Dismutase ◽  
Reperfusion Injury ◽  
Knockout Mice ◽  
Extracellular Superoxide Dismutase

Effects of swimming training on three superoxide dismutase isoenzymes in mouse tissues

2000 ◽  
Vol 88 (2) ◽  
pp. 649-654 ◽  
Author(s):  
Chitose Nakao ◽  
Tomomi Ookawara ◽  
Takako Kizaki ◽  
Shuji Oh-Ishi ◽  
Hiromi Miyazaki ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Skeletal Muscle ◽  
Superoxide Dismutase ◽  
Gastrocnemius Muscle ◽  
Mrna Abundance ◽  
Mrna Levels ◽  
Swimming Training ◽  
Protein Levels ◽  
Mouse Tissues ◽  
Liver And Kidney

The purpose of the present study was to investigate the effects of swimming training on the changes in three superoxide dismutase (SOD) isoenzymes in mice. The trained mice underwent a 6-wk swimming program (1 h/day, 5 days/wk) in water at 35–36°C. Immunoreactive extracellular SOD (EC-SOD), copper- and zinc-containing SOD (CuZn-SOD), and manganese-containing SOD (Mn-SOD) contents and their mRNA abundance were determined in serum, heart, lung, liver, kidney, and gastrocnemius muscle. EC-SOD content in liver and kidney was significantly increased with training. After training, CuZn-SOD content rose significantly only in kidney but decreased significantly in heart, lung, and liver. Mn-SOD content showed a significant increase in lung, kidney, and skeletal muscle but a significant decrease in liver. In most tissues, however, the changes in SOD isoenzyme contents were not concomitant with those in their mRNA levels. The results obtained thus suggest that, except for kidney, the responses in mouse tissues of three SOD isoenzymes (protein levels and mRNA abundance) to swimming training are different and that kidney may be one of the most sensitive organs to adapt to oxidative stress during physical training, although the mechanism remains vague.

scholarly journals PO-282 Effects Of Irises On Exercise To Improve Obesity

2018 ◽  
Vol 1 (5) ◽  
Author(s):  
Weihua Zhu
Keyword(s):  
Insulin Resistance ◽  
Skeletal Muscle ◽  
Metabolic Syndrome ◽  
Exercise Intensity ◽  
Exercise Intervention ◽  
Brown Fat ◽  
Research Progress ◽  
Original Research ◽  
Peroxisome Proliferator ◽  
White Fat

Objective Iirisin is a protein encoded by the target gene FNDC5 of peroxisome proliferator-activated receptor gamma coactivator in skeletal muscle. The secretory protein produced by shearing modification can promote the transformation of subcutaneous white fat into brown fat. As a kind of exercise-mediated muscle factor and potential fat factor, Irisin is closely related to obesity, insulin resistance, glucose and lipid metabolism, metabolic syndrome and so on. As an important preventive and therapeutic means of obesity, exercise plays a role in affecting irisin? Through the analysis of the effect of irisin on exercise intervention in obesity, this paper aims to lay a theoretical foundation for irisin to become a new way of thinking and a new target of treatment of obesity. Methods A computer-based search of the literature on "Research Progress in the Effects of Irisin on Exercise-induced Obesity" was conducted in the Sportdiscussussussand CNKI databases from 2012 to 2017. The key words were "obesity; exercise; Irisin; brown fat". Inclusion criteria were original research, Meta analysis and systematic review. Exclusion criteria: repetitive studies. A total of 86 articles were included in the review. Results (1) exercise can significantly improve obesity, insulin resistance, metabolic syndrome and other diseases;(2) Irisin can induce white fat Browning, increase body heat production, reduce body weight, and promote the expression of UCP1 by p38MAPK/ERK signaling pathway. Exogenous Irisin can significantly reduce obesity in mice induced by high-fat diet and improve insulin resistance.(3) whether it is one-time exercise or long-term exercise, endurance exercise or resistance exercise, moderate and low-intensity exercise or high-intensity exercise will increase the expression of irisin in skeletal muscle, blood or fat.However, the influence of different exercise intensity and different exercise modes on the expression of irisin is not regular, and the influence and mechanism of different exercise modes and exercise intensity on the expression of irisin between different species and different tissues have not been reported. Conclusions Exercise can significantly improve the occurrence and development of obesity, and the effect may be achieved by promoting the secretion and expression of irisin in skeletal muscle.

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