Rate of tension redevelopment is not modulated by sarcomere length in permeabilized human, murine, and porcine cardiomyocytes

2007 ◽  
Vol 293 (1) ◽  
pp. R20-R29 ◽  
Author(s):  
István Ferenc Édes ◽  
Dániel Czuriga ◽  
Gábor Csányi ◽  
Stefan Chłopicki ◽  
Fabio A. Recchia ◽  
...  
Keyword(s):  
Sarcomere Length ◽  
Species Differences ◽  
Isometric Force ◽  
Force Production ◽  
Major Influence ◽  
Turnover Rates ◽  
Force Development ◽  
Cross Bridge ◽  
The Cross ◽  
Isometric Force Production

The increase in Ca2+ sensitivity of isometric force development along with sarcomere length (SL) is considered as the basis of the Frank-Starling law of the heart, possibly involving the regulation of cross-bridge turnover kinetics. Therefore, the Ca2+ dependencies of isometric force production and of the cross-bridge-sensitive rate constant of force redevelopment ( ktr) were determined at different SLs (1.9 and 2.3 μm) in isolated human, murine, and porcine permeabilized cardiomyocytes. ktr was also determined in the presence of 10 mM inorganic phosphate (Pi), which interfered with the force-generating cross-bridge transitions. The increases in Ca2+ sensitivities of force with SL were very similar in human, murine, and porcine cardiomyocytes (ΔpCa50: ∼0.11). ktr was higher ( P < 0.05) in mice than in humans or pigs at all Ca2+ concentrations ([Ca2+]) [maximum ktr ( ktr,max) at a SL of 1.9 μm and pCa 4.75: 1.33 ± 0.11, 7.44 ± 0.15, and 1.02 ± 0.05 s−1, in humans, mice, and pigs, respectively] but ktr did not depend on SL in any species. Moreover, when the ktr values for each species were expressed relative to their respective maxima, similar Ca2+ dependencies were obtained. Ten millimolar Pi decreased force to ∼60–65% and left ΔpCa50 unaltered in all three species. Pi increased ktr,max by a factor of ∼1.6 in humans and pigs and by a factor of ∼3 in mice, independent of SL. In conclusion, species differences exert a major influence on ktr, but SL does not appear to modulate the cross-bridge turnover rates in human, murine, and porcine hearts.

The Frank-Starling mechanism is not mediated by changes in rate of cross-bridge detachment

1997 ◽  
Vol 273 (5) ◽  
pp. H2428-H2435 ◽  
Author(s):  
Thomas Wannenburg ◽  
Paul M. L. Janssen ◽  
Dongsheng Fan ◽  
Pieter P. De Tombe
Keyword(s):  
Cardiac Muscle ◽  
Maximum Rate ◽  
Sarcomere Length ◽  
Myosin Head ◽  
Isometric Force ◽  
Force Development ◽  
Cross Bridge ◽  
Average Slope ◽  
The Cross ◽  
Kinetics Of

We tested the hypothesis that the Frank-Starling relationship is mediated by changes in the rate of cross-bridge detachment in cardiac muscle. We simultaneously measured isometric force development and the rate of ATP consumption at various levels of Ca2+ activation in skinned rat cardiac trabecular muscles at three sarcomere lengths (2.0, 2.1, and 2.2 μm). The maximum rate of ATP consumption was 1.5 nmol ⋅ s−1 ⋅ μl fiber vol−1, which represents an estimated adenosinetriphosphatase (ATPase) rate of ∼10 s−1 per myosin head at 24°C. The rate of ATP consumption was tightly and linearly coupled to the level of isometric force development, and changes in sarcomere length had no effect on the slope of the force-ATPase relationships. The average slope of the force-ATPase relationships was 15.5 pmol ⋅ mN−1 ⋅ mm−1. These results suggest that the mechanisms that underlie the Frank-Starling relationship in cardiac muscle do not involve changes in the kinetics of the apparent detachment step in the cross-bridge cycle.

scholarly journals Force generated by myosin cross-bridges is reduced in myofibrils exposed to ROS/RNS

2019 ◽  
Vol 317 (6) ◽  
pp. C1304-C1312 ◽  
Author(s):  
Malin Persson ◽  
Maarten M. Steinz ◽  
Håkan Westerblad ◽  
Johanna T. Lanner ◽  
Dilson E. Rassier
Keyword(s):  
Oxidative Stress ◽  
Skeletal Muscle ◽  
Posttranslational Modifications ◽  
Isometric Force ◽  
Force Production ◽  
Indirect Evidence ◽  
Cross Bridge ◽  
Atomic Force ◽  
The Cross ◽  
Cross Bridges

Skeletal muscle weakness is associated with oxidative stress and oxidative posttranslational modifications on contractile proteins. There is indirect evidence that reactive oxygen/nitrogen species (ROS/RNS) affect skeletal muscle myofibrillar function, although the details of the acute effects of ROS/RNS on myosin-actin interactions are not known. In this study, we examined the effects of peroxynitrite (ONOO−) on the contractile properties of individual skeletal muscle myofibrils by monitoring myofibril-induced displacements of an atomic force cantilever upon activation and relaxation. The isometric force decreased by ~50% in myofibrils treated with the ONOO− donor (SIN-1) or directly with ONOO−, which was independent of the cross-bridge abundancy condition (i.e., rigor or relaxing condition) during SIN-1 or ONOO− treatment. The force decrease was attributed to an increase in the cross-bridge detachment rate ( gapp) in combination with a conservation of the force redevelopment rate (kTr) and hence, an increase in the population of cross-bridges transitioning from force-generating to non-force-generating cross-bridges during steady-state. Taken together, the results of this study provide important information on how ROS/RNS affect myofibrillar force production which may be of importance for conditions where increased oxidative stress is part of the pathophysiology.

Mysteries of Muscle Contraction

2008 ◽  
Vol 24 (1) ◽  
pp. 1-13 ◽  
Author(s):  
Walter Herzog ◽  
Timothy R. Leonard ◽  
Venus Joumaa ◽  
Ashi Mehta
Keyword(s):  
Steady State ◽  
Experimental Evidence ◽  
Active Component ◽  
Isometric Force ◽  
Force Production ◽  
Myosin Filament ◽  
Passive Component ◽  
Cross Bridge ◽  
The Cross ◽  
State Force

According to the cross-bridge theory, the steady-state isometric force of a muscle is given by the amount of actin–myosin filament overlap. However, it has been known for more than half a century that steady-state forces depend crucially on contractile history. Here, we examine history-dependent steady-state force production in view of the cross-bridge theory, available experimental evidence, and existing explanations for this phenomenon. This is done on various structural levels, ranging from the intact muscle to the myofibrillar and isolated contractile protein level, so that advantages and limitations of the various preparations can be fully exploited and overcome. Based on experimental evidence, we conclude that steady-state force following active muscle stretching is enhanced, and this enhancement has a passive and an active component. The active component is associated with the cross-bridge kinetics, and the passive component is associated with a calcium-dependent increase in titin stiffness.

Feedback about isometric force production yields more random variations

2012 ◽  
Vol 513 (1) ◽  
pp. 37-41 ◽  
Author(s):  
Dilip N. Athreya ◽  
Guy Van Orden ◽  
Michael A. Riley
Keyword(s):  
Isometric Force ◽  
Force Production ◽  
Isometric Force Production

Acute beetroot juice administration improves peak isometric force production in adolescent males

2018 ◽  
Vol 43 (8) ◽  
pp. 816-821 ◽  
Author(s):  
David Bender ◽  
Jeremy R. Townsend ◽  
William C. Vantrease ◽  
Autumn C. Marshall ◽  
Ruth N. Henry ◽  
...  
Keyword(s):  
Isometric Force ◽  
Force Production ◽  
Sprint Performance ◽  
Peak Force ◽  
Adolescent Males ◽  
Height Velocity ◽  
Beetroot Juice ◽  
Repeated Sprint ◽  
Group Time ◽  
Isometric Force Production

The purpose of this study was to examine the effects of acute beetroot juice (BR) administration on repeated sprint performance and isometric force production in adolescent males. Twelve male adolescents (age, 16.8 ± 1.0 years; height, 178.8 ± 9.2 cm; mass, 74.8 ± 12.5 kg; peak height velocity, 2.53 ± 1.2 years) participated in this double-blind, placebo-controlled, crossover designed study. Participants consumed 2 × 70 mL of BR (∼12.9 mmol NO3−; Beet It Sport) or a nitrate-depleted placebo (PL) at 2.5 h prior to performing isometric mid-thigh pulls (IMTP) and 4 repeated 20-s Wingate sprints interspersed with 4 min of rest. Sprint data were analyzed by a 2 × 4 (group × time) repeated-measures ANOVA while a dependent t test was used to compare conditions for IMTP peak force. A significant main effect for time (p < 0.05) was observed for peak power (PP), average power (Pavg), and fatigue index (FI) across sprints. Compared with sprint 1, sprint 4 resulted in significant decreases in PP (p < 0.000; −16.6%) and Pavg (p = 0.000; −21.8%) and FI was significantly elevated (p < 0.000; 15.2%). No significant group × time interactions were observed between conditions for PP (p = 0.402), Pavg (p = 0.479), or FI (p = 0.37). IMTP peak force was significantly higher (p = 0.004; 13.9%) following BR consumption compared with PL. The repeated sprint protocol resulted in significant fatigue while BR did not influence sprint performance. However, it appears BR administration may improve peak force production in adolescent males.

scholarly journals Force enhancement after stretch of isolated myofibrils is increased by sarcomere length non-uniformities

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Ricarda M. Haeger ◽  
Dilson E. Rassier
Keyword(s):  
Steady State ◽  
Sarcomere Length ◽  
Isometric Force ◽  
Force Production ◽  
Force Enhancement ◽  
Residual Force ◽  
Residual Force Enhancement ◽  
State Force ◽  
Isometric Forces

AbstractWhen a muscle is stretched during a contraction, the resulting steady-state force is higher than the isometric force produced at a comparable sarcomere length. This phenomenon, also referred to as residual force enhancement, cannot be readily explained by the force-sarcomere length relation. One of the most accepted mechanisms for the residual force enhancement is the development of sarcomere length non-uniformities after an active stretch. The aim of this study was to directly investigate the effect of non-uniformities on the force-producing capabilities of isolated myofibrils after they are actively stretched. We evaluated the effect of depleting a single A-band on sarcomere length non-uniformity and residual force enhancement. We observed that sarcomere length non-uniformity was effectively increased following A-band depletion. Furthermore, isometric forces decreased, while the percent residual force enhancement increased compared to intact myofibrils (5% vs. 20%). We conclude that sarcomere length non-uniformities are partially responsible for the enhanced force production after stretch.

The adaptive range of 1/f isometric force production.

2009 ◽  
Vol 35 (2) ◽  
pp. 439-446 ◽  
Author(s):  
Jacob J. Sosnoff ◽  
Andrew D. Valantine ◽  
Karl M. Newell
Keyword(s):  
Isometric Force ◽  
Force Production ◽  
Isometric Force Production
Sign in / Sign up

Export Citation Format

Share Document