CCK/gastrin-like immunoreactivity in brain and gut, and CCK suppression of feeding in goldfish

1994 ◽  
Vol 267 (3) ◽  
pp. R841-R851 ◽  
Author(s):  
B. A. Himick ◽  
R. E. Peter
Keyword(s):  
Feeding Behavior ◽  
Endocrine Cells ◽  
Brain Regions ◽  
Nerve Fibers ◽  
Serum Growth Hormone ◽  
Ventral Telencephalon ◽  
The Third ◽  
Brain Ventricle ◽  
Feeding Center ◽  
First Time

The presence and distribution of cholecystokinin (CCK)/gastrin-like immunoreactive (IR) material were examined in the goldfish brain and gut. In the forebrain, CCK/gastrin-like IR fibers and perikarya were localized to nuclei of predominantly the ventral telencephalon and diencephalon; more dorsal forebrain regions contained only few, thin-beaded, sparsely distributed IR fibers. CCK/gastrin-like IR was specifically detected in the preoptic hypothalamus, including the nucleus entopeduncularis, nucleus preopticus periventricularis, and nucleus preopticus. Of all brain regions examined, the highest concentration of CCK/gastrin-like IR staining was consistently observed in nuclei of the ventroposterior and inferior lobes of the hypothalamus. Within the gut, both nerve fibers and endocrine cells contained CCK/gastrin-like IR. The abundance of CCK/gastrin-like IR material within brain areas known to represent the feeding center of fish, as well as the presence of IR material within the gut, suggests that CCK may influence feeding behavior in fish. When injected either intraperitoneally or into the third brain ventricle into goldfish, sulfated CCK-8 (CCK-8s) suppressed food intake. The nonsulfated form of CCK-8 was not as effective as CCK-8s in suppressing feeding after intraperitoneal injection. No consistent changes were observed in circulating serum growth hormone or gonadotropin levels after either intraperitoneal or intracerebroventricular injection of CCK-8s. These studies demonstrate for the first time that CCK-8s is effective in acutely suppressing feeding behavior in fish when administered either peripherally or centrally into the third brain ventricle.

scholarly journals Study on the pathogenesis of Holmes tremor by multimodal 3D medical imaging: case reports of three patients

BMC Neurology ◽  
2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Min Shi ◽  
Anrong Wang ◽  
Yu Fang ◽  
Jun Guo ◽  
Zhaoying Li ◽  
...  
Keyword(s):  
Medical Imaging ◽  
Disease Onset ◽  
Nerve Fibers ◽  
The Other ◽  
Therapeutic Effects ◽  
Thalamic Nuclei ◽  
The Third ◽  
Holmes Tremor ◽  
Lateral Nucleus ◽  
First Time

Abstract Background We examined for the first time the imaging characteristics of Holmes tremor (HT) through multimodal 3D medical imaging. Case presentation Three patients with Holmes tremor who visited the Affiliated Hospital of Chengdu University of TCM from August 2018 to April 2021 were retrospectively investigated to summarize their clinical and imaging data. Results Holmes tremor in two of the three patients was caused by hypertensive cerebral hemorrhage and in the third patient induced by hemorrhage due to ruptured brain arteriovenous malformations. HT occurred 1 to 24 months after the primary disease onset and manifested as a tremor in the contralateral limb, mostly in the upper portion. Cranial MRI showed that the lesions involved the thalamus in all three patients. The damaged thalamic nuclei included the ventral anterior nucleus, ventral lateral nucleus and ventromedial lateral nucleus, and the damaged nerve fibers included left thalamocortical tracts in one patient. In the other two patients, the damaged thalamic nuclei included the centromedian and dorsomedial nucleus, and the damaged nerve fibers included left cerebellothalamic and thalamocortical tracts. One patient showed significant improvement after treatment with pramipexole while the other two patients exhibited a poor response, one of whom had no response to the treatment with pramipexole and was only significantly relieved by clonazepam. Conclusion We used multimodal 3D medical imaging for the first time to analyze the pathogenesis of HT and found that multiple thalamic nuclei were damaged. The damaged nuclei and nerve fiber tracts of two patients were different from those of the third patient, with different clinical manifestations and therapeutic effects. Therefore, it is speculated that there may be multiple pathogeneses for HT.

The innervation of the human antro-pyloric region: Organization and composition

2001 ◽  
Vol 79 (11) ◽  
pp. 905-918 ◽  
Author(s):  
V C Smith ◽  
N Dhatt ◽  
A MJ Buchan
Keyword(s):  
Nitric Oxide ◽  
Endocrine Cells ◽  
Nerve Fibers ◽  
Pyloric Sphincter ◽  
Submucosal Plexus ◽  
Pyloric Region ◽  
Immunocytochemical Techniques ◽  
Functional Regulation ◽  
Mucosal Innervation ◽  
First Time

Although the composition of the gastric innervation has been determined in animal models, relatively little known about the innervation of the human antro-pyloric region. We used immunocytochemical techniques to establish the localization and co-expression of neuropeptides and nitric oxide in the human antrum and upper duodenum. Our results demonstrate the existence of a clearly defined submucosal plexus in the antral region that is absent in rats and guinea pigs. The abundant innervation of the lamina propria contains 3 major nerve populations: VIP- and NOS-, SP- and CGRP-, and GRP-immunoreactive. For the first time, NOS-containing nerve fibers were observed throughout the length of the antral glands. Within the antrum somatostatin was confined to endocrine cells, however, at the pyloric sphincter both enteric plexi contained immunoreactive neurons and nerve fibres. Within the pyloric sphincter CGRP- and SP-immunoreactive fibres were significantly increased, correlating with the presence of large ganglia in the submucosal plexus. In conclusion, the organization and composition of the innervation of human antro-pylorus differed substantially from that reported in other mammals. The presence of an abundant mucosal innervation paralled by a well-defined submucosal plexus indicates that the functional regulation of the gastric–pyloric region will be distinct from that of smaller animal models.Key words: gastric innervation, pyloric sphincter, neuropeptides, nitric oxide, somatostatin.

scholarly journals MELANOPHORE BANDS AND AREAS DUE TO NERVE CUTTING, IN RELATION TO THE PROTRACTED ACTIVITY OF NERVES

1941 ◽  
Vol 24 (4) ◽  
pp. 483-504 ◽  
Author(s):  
G. H. Parker
Keyword(s):  
Pituitary Gland ◽  
Olive Oil ◽  
Magnesium Sulfate ◽  
Nerve Fibers ◽  
Autonomic Nerve ◽  
Color Changes ◽  
The Third ◽  
Limited Degree ◽  
Adrenergic Fibers

1. When appropriate chromatic nerves are cut caudal bands, cephalic areas, and the pelvic fins of the catfish Ameiurus darken. In pale fishes all these areas will sooner or later blanch. By recutting their nerves all such blanched areas will darken again. 2. These observations show that the darkening of caudal bands, areas, and fins on cutting their nerves is not due to paralysis (Brücke), to the obstruction of central influences such as inhibition (Zoond and Eyre), nor to vasomotor disturbances (Hogben), but to activities emanating from the cut itself. 3. The chief agents concerned with the color changes in Ameiurus are three: intermedin from the pituitary gland, acetylcholine from the dispersing nerves (cholinergic fibers), and adrenalin from the concentrating nerves (adrenergic fibers). The first two darken the fish; the third blanches it. In darkening the dispersing nerves appear to initiate the process and to be followed and substantially supplemented by intermedin. 4. Caudal bands blanch by lateral invasion, cephalic areas by lateral invasion and internal disintegration, and pelvic fins by a uniform process of general loss of tint equivalent to internal disintegration. 5. Adrenalin may be carried in such an oil as olive oil and may therefore act as a lipohumor; it is soluble in water and hence may act as a hydrohumor. In lateral invasion (caudal bands, cephalic areas) it probably acts as a lipohumor and in internal disintegration (cephalic areas, pelvic fins) it probably plays the part of a hydrohumor. 6. The duration of the activity of dispersing nerves after they had been cut was tested by means of the oscillograph, by anesthetizing blocks, and by cold-blocks. The nerves of Ameiurus proved to be unsatisfactory for oscillograph tests. An anesthetizing block, magnesium sulfate, is only partly satisfactory. A cold-block, 0°C., is successful to a limited degree. 7. By means of a cold-block it can be shown that dispersing autonomic nerve fibers in Ameiurus can continue in activity for at least 6½ hours. It is not known how much longer they may remain active. So far as the duration of their activity is concerned dispersing nerve fibers in this fish are unlike other types of nerve fibers usually studied.

Early Permian fossil vertebrate footprints and their stratigraphic setting in megacyclic sequence II red beds, Prim Point, Prince Edward Island

1989 ◽  
Vol 26 (3) ◽  
pp. 591-605 ◽  
Author(s):  
David J. Mossman ◽  
Craig H. Place
Keyword(s):  
Central Europe ◽  
Prince Edward Island ◽  
River Sediments ◽  
Early Permian ◽  
Red Beds ◽  
Sparse Vegetation ◽  
The Third ◽  
Fossil Vertebrate ◽  
Crevasse Splay ◽  
First Time

Vertebrate trace fossils are reported for the first time from red beds near the top of megacyclic sequence II at Prim Point in southwestern Prince Edward Island. They occur as casts of tetrapod trackways. The ichnocoenose also includes a rich invertebrate ichnofauna. The trackmakers thrived in an area of sparse vegetation and occupied out-of-channel river sediments, most likely crevasse-splay deposits.Amphisauropus latus, represented by three trackways, has been previously reported from Germany, Poland, and Czechoslovakia. It is here interpreted as the track of a cotylosaur. It occurs together with the track of Gilmoreichnus kablikae, which is either a captorhinomorph or possibly a juvenile pelycosaur. These facilitate the assignment of a late Early Permian (late Autunian) age to the strata. The third set of footprints, those of a small herbivorous pelycosaur, compare most closely with Ichniotherium willsi, known hitherto from the Keele beds (latest Stephanian) of the English Midlands.This ichnocoenose occurs in a plate-tectonically rafted segment of crust stratigraphically equivalent to the same association of ichnofauna in the English Midlands and central Europe. The community occupied piedmont-valley-flat red beds within the molasse facies of Variscan uplands.

scholarly journals Characterization of the inositol 1,4,5-trisphosphate-induced calcium release from permeabilized endocrine cells and its inhibition by decavanadate and p-hydroxymercuribenzoate

1989 ◽  
Vol 262 (1) ◽  
pp. 83-89 ◽  
Author(s):  
K J Föhr ◽  
J Scott ◽  
G Ahnert-Hilger ◽  
M Gratzl
Keyword(s):  
Endocrine Cells ◽  
Calcium Release ◽  
Cell Types ◽  
Inhibitory Effect ◽  
Maximal Effect ◽  
Thiol Compounds ◽  
Inositol 1,4,5 Trisphosphate ◽  
Dependent Inhibition ◽  
Pheochromocytoma Pc12 ◽  
First Time

The inositol 1,4,5-trisphosphate (IP3)-sensitive Ca2+ compartment of endocrine cells was studied with alpha-toxin- and digitonin-permeabilized rat insulinoma (RINA2) and rat pheochromocytoma (PC12) cells. The Ca2+ uptake was ATP-dependent, and submicromolar concentrations of IP3 specifically released the stored Ca2+. Half-maximal Ca2+ release was observed with 0.25-0.5 mumol of IP3/l, and the amount of Ca2+ released due to IP3 could be enhanced by additional loading of the Ca2+ compartment. Consecutive additions of the same concentration of IP3 for 1-2 h always released the same amount of Ca2+ without desensitization, providing an ideal basis to further characterize the IP3-induced Ca2+ release. Here we describe for the first time a reversible inhibitory effect of decavanadate on the IP3-induced Ca2+ release. Among the vanadium species tested (decavanadate, oligovanadate and monovanadate), only decavanadate was inhibitory, with a half-maximal effect at 5 mumol/l in both cell types. The effect of decavanadate could be overcome by increasing the amount of sequestered Ca2+ or added IP3. Decavanadate did not affect the ATP-driven Ca2+ uptake but oligovanadate was inhibitory on Ca2+ uptake. p-Hydroxymercuribenzoate (pHMB) at concentrations between 10 and 30 mumol/l also inhibited the Ca2+ release due to IP3. Thiol compounds such as dithiothreitol (DTT; 1 mmol/l) added before pHMB removed all its inhibitory effect on the IP3-induced Ca2+ release, whereas the inhibition caused by decavanadate was unaffected by DTT. Thus, the decavanadate-dependent inhibition functions by a distinctly different mechanism than pHMB and could serve as a specific tool to analyse various aspects of the IP3-induced Ca2+ release within endocrine cells.

Production and Technical Characteristics of a New Class of Amorphous Metals

1995 ◽  
Vol 400 ◽  
Author(s):  
R.T. Malkhassian
Keyword(s):  
Amorphous Phase ◽  
Quantum Chemical ◽  
New Technology ◽  
Chemical Technology ◽  
Quantum Level ◽  
Vibrationally Excited ◽  
New Class ◽  
The Third ◽  
Hydrogen Molecules ◽  
First Time

AbstractA new technology for obtainment of amorphous single-component metals is presented.For the first time the reduction of molybdenum oxide with formation of its amorphous phase is realized in conditions of a given quantum-chemical technology by means of vibrationally excited to the third quantum level hydrogen molecules with 1.5 ± 0.2 eV energy. The evidences of formation of this nonequilibrium amorphous phase are presented along with certain physicochemical properties of the obtained amorphous molybdenum.A model is proposed for the origin of amorphous phase under the influence of nonequilibrium quantum-chemical technology.

Early (Avant-garde) Symphonies by Valentin Silvestrov As a Sound Universe

2021 ◽  
Vol 66 (1) ◽  
pp. 329-343
Author(s):  
Liudmyla Shapovalova ◽  
Іryna Romaniuk ◽  
Marianna Chernyavska ◽  
Svitlana Shchelkanova
Keyword(s):  
20Th Century ◽  
Human Being ◽  
Accurate Identification ◽  
Philosophy Of Music ◽  
Philosophical Concept ◽  
Avant Garde ◽  
The Third ◽  
Ninth Symphony ◽  
Human Ontology ◽  
First Time

"In the article under consideration are the ways of symphony genre transformation in the early works of Valentin Silvestrov (Ukraine). For the first time, the First, Second, Third, and Fourth symphonies by the genius composers of the 20th century are analyzed as a certain stylistic system. These compositions are endowed with the features of avant-garde poetics, and as a subject of musicological reflection, they are associated with a rethinking of the semantic paradigm of the genre. V. Silvestrov's early symphonies stand out from the classical practice of European symphonies. Scientific awareness of their phenomenal nature necessitated a methodological choice aimed at the most accurate identification of the philosophical concept of the new sound universum of V. Silvestrov's music. Deep correlation of the image of a human being as a factor of the symphony poetics (the influence of philosophical concepts of human ontology in the 20th century with the transformation of the genre canon) is considered. This refers to the nonmusical dimension of the genre semantics. The study of V. Silvestrov's early symphonies reveal a new philosophy of music through gradual movement – modulation: from the neo-baroque First Symphony and ""cosmic pastorals"" Musica Mundana of the Second Symphony through the history anthropologisation in the Third Symphony ""Eschatology"" to the monodrama Musica Humana in the Fourth Symphony. The dichotomy of Musica Mundana – Musica Humana is not accidental: in V. Silvestrov's creative method, remains relevant, which is confirmed by the dramaturgy of his latest work – the Ninth symphony (2019). Keywords: V. Silvestrov's early symphonies, evolution of style, worldview, Musica Mundana, monodrama. "

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