Blood and brain magnesium in inbred mice and their correlation with sleep quality

A strong genetic component in the regulation of blood magnesium (Mg) levels has been demonstrated. The regulation and distribution of brain Mg levels, however, have never been assessed. Herein we report on the genetic variation of peripheral and central Mg levels in six inbred strains of mice. In addition, the possible involvement of Mg in sleep regulation was assessed by establishing correlations between Mg and sleep parameters obtained before and after a 6-h sleep deprivation. Although genotype strongly determined blood Mg levels, it did not affect brain Mg, suggesting that central and peripheral Mg are regulated differently. Central Mg displayed a highly structure-specific distribution with frontal cortex having the highest and brain stem the lowest values. Whereas for the amount and distribution of baseline sleep only marginal correlations with Mg were found, Mg contents in four of nine brain structures were highly positively correlated with the length of slow-wave sleep episodes during recovery. This relationship suggests that higher levels of Mg in specific brain sites promote sleep quality as part of a recovery process.

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430 Outcome of Sleep-Wake Pattern in Obstructive Sleep Apnea Patients after Positive Airway Pressure Therapy using Actigraphy

SLEEP ◽

10.1093/sleep/zsab072.429 ◽

2021 ◽

Vol 44 (Supplement_2) ◽

pp. A170-A170

Author(s):

Narumol Luekitinun ◽

Kanlaya Panjapornpon ◽

Pattharaphong Plurksathaporn ◽

Rungaroon Tangsrikertikul ◽

Wanlana Tongkien ◽

...

Keyword(s):

Obstructive Sleep Apnea ◽

Sleep Apnea ◽

Sleep Quality ◽

Airway Pressure ◽

Positive Airway Pressure ◽

Matched Pair ◽

Cpap Therapy ◽

Obstructive Sleep ◽

Before And After ◽

Sleep Parameters

Abstract Introduction The initial phase of continuous positive airway pressure (CPAP) therapy in patients with obstructive sleep apnea (OSA) may affect sleep parameters and PAP compliance. Actigraphy is a validated method using accelerometer to objectively measure sleep parameters in patients with a range of sleep disorders, including OSA, particularly to follow-up after treatment. We compare sleep parameters from actigraphy, sleep log, sleep diary, Epworth Sleepiness Scale (ESS), Pittsburgh Sleep Quality Index (PSQI), heart rate and blood pressure in OSA patients before and after CPAP therapy for 1 week. Methods This pilot study have been prospectively performed at Central Chest Institute of Thailand (CCIT) since June to November 2020. Adult OSA patients, diagnosed by ICSD-3 criteria and achieved optimal or good CPAP pressure titration from split-night polysomnography (PSG), were informed and consent to wear actigraphy before and after CPAP therapy each for 1 week. Clinical and sleep parameters were recorded and analyzed using Wilcoxon matched-pair signed-rank and Mann Whitney U test. P-value < 0.05 was considered to have statistical significance. Results All 11 OSA patients participated in this study. Most patients were male (63.6%), hypertension (54.5%) and dyslipidemia (45.4%). Means of age, body mass index (BMI), ESS, PSQI, apnea hypopnea index (AHI), nadir SpO2, and CPAP usage were 45.5 ± 15.9 years, 29.1 ± 5.2 kg/m2, 10.8 ± 3.9, 7.7 ± 2.9, 65.2 ± 37.7 events/h, 82.3 ± 10.8 % and 9.5 ± 3.1 cmH2O, respectively. Comparing before and after 1-week CPAP therapy, an average number of wake bouts ((NWB), 48.4 vs 38 events, p=0.010), ESS (11 vs 9, p=0.035) and PSQI (8 vs 4, p=0.005) were significantly decrease. Additionally, when comparing between poor and good CPAP compliance group, NWB (55.1 vs 36.3 events, p=0.036) and the difference of wake after sleep onset (WASO, 10.5 vs -0.11 min, p=0.035) were significantly decrease. Conclusion OSA patients treated with CPAP for 1-week experienced marked improvement in sleepiness, sleep quality and nighttime awakening. Support (if any):

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Sleep is differently modulated by basal forebrain GABAA and GABAB receptors

AJP Regulatory Integrative and Comparative Physiology ◽

10.1152/ajpregu.2001.281.1.r170 ◽

2001 ◽

Vol 281 (1) ◽

pp. R170-R175 ◽

Cited By ~ 21

Author(s):

Alfredo Manfridi ◽

Dario Brambilla ◽

Mauro Mancia

Keyword(s):

Basal Forebrain ◽

Slow Wave Sleep ◽

Gabab Receptors ◽

Nucleus Basalis ◽

Receptor Subtype ◽

Sleep Regulation ◽

Sleep Parameters ◽

Polygraphic Recordings ◽

Sleep Modulation

There is evidence that GABA plays a major role in sleep regulation. GABAA receptor agonists and different compounds interacting with the GABAA receptor complex, such as barbiturates and benzodiazepines, can interfere with the sleep/wake cycle. On the other hand, there is very little information about the possible role of GABAB receptors in sleep modulation. The nucleus basalis of Meynert (NBM), a cholinergic area in the basal forebrain, plays a pivotal role in the modulation of sleep and wakefulness, and both GABAA and GABABreceptors have been described within the NBM. This study used unilateral infusions in the NBM to determine the effects of 3-hydroxy-5-aminomethylisoxazole hydrobromide (muscimol hydrobromide, a GABAA receptor subtype agonist) and β-(aminomethyl)-4-chlorobenzenepropanoic acid (baclofen, a GABAB receptor subtype agonist) on sleep parameters in freely moving rats by means of polygraphic recordings. Muscimol (0.5 nmol) and baclofen (0.7 nmol) induced an increase in slow-wave sleep and an inhibition of wakefulness. Muscimol, but not baclofen, also caused a decrease in desynchronized sleep parameters. The results reported here indicate that 1) the NBM activation of both GABAA and GABAB receptors influences the sleep/wake cycle, and 2) GABAA but not GABAB receptors are important for desynchronized sleep modulation, suggesting that the two GABAergic receptors play different roles in sleep modulation.

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mRNA expression in mouse hypothalamus and basal forebrain during influenza infection: a novel model for sleep regulation

Physiological Genomics ◽

10.1152/physiolgenomics.00005.2005 ◽

2006 ◽

Vol 24 (3) ◽

pp. 225-234 ◽

Cited By ~ 8

Author(s):

Ming Ding ◽

Linda A. Toth

Keyword(s):

Basal Forebrain ◽

Slow Wave Sleep ◽

Inbred Mice ◽

Influenza Infection ◽

Dark Phase ◽

Potential Candidate ◽

Lipocalin 2 ◽

Sleep Regulation ◽

Light Phase ◽

Potential Candidate Gene

After influenza infection, C57BL/6J mice develop increased slow-wave sleep (SWS) during the dark phase of the day-night cycle, whereas BALB/cByJ mice develop decreased SWS during the light phase. A previous analysis of CXB recombinant inbred mice revealed a quantitative trait locus (QTL) designated Srilp (sleep response to influenza, light phase) that was related to expression of the BALB/cByJ sleep phenotype. Srilp was localized to the 10- to 12-cM region of mouse Chr 6 between D6Mit74 and D6Mit188. Temt (thioether S-methyltransferase), which is located at region B3 of Chr 6, is a potential candidate gene for Srilp. We evaluated the expression of Temt and other Srilp candidate genes in hypothalamus and basal forebrain of uninfected and influenza-infected C57BL/6J and BALB/cByJ mice. We report here that Temt expression varies significantly with respect to mouse strain, health status, brain region, and day-night phase. C57BL/6J mice show day-night variation in Temt expression in hypothalamus, but BALB/cByJ mice do not. Temt expression in basal forebrain is much higher in C57BL/6J mice than in BALB/cByJ mice. During influenza infection, both C57BL/6J and BALB/cByJ mice show reduced Temt mRNA in basal forebrain at 30 h postinoculation, but expression remains much lower in the BALB/cByJ strain. In contrast, prostaglandin-d-synthase ( Ptgds) and lipocalin 2 ( Lcn2) mRNA increase in basal forebrain of both strains after influenza infection. Administration of the TEMT inhibitor sinefungin reduces sleep in uninfected BALB/cByJ mice and attenuates influenza-induced sleep enhancement in C57BL/6J mice. These data suggest that strain- and infection-related alterations in sleep may be influenced by Temt expression and perhaps by subsequent effects on prostaglandin metabolism.

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Polysomnographic Findings in Nights Preceding a Migraine Attack

Cephalalgia ◽

10.1046/j.1468-2982.2001.00141.x ◽

2001 ◽

Vol 21 (1) ◽

pp. 31-37 ◽

Cited By ~ 63

Author(s):

R Göder ◽

G Fritzer ◽

A Kapsokalyvas ◽

P Kropp ◽

U Niederberger ◽

...

Keyword(s):

Slow Wave Sleep ◽

Power Spectra ◽

Cortical Activation ◽

Alpha Power ◽

Sleep Regulation ◽

Beta Power ◽

Rem Density ◽

Sleep Parameters ◽

Sleep Alterations ◽

Electroencephalogram Eeg

Sleep recordings were performed in eight patients to analyse sleep alterations preceding migraine attacks. Polysomnographic recordings from nights before an attack were compared with nights without following migraine. We analysed standard sleep parameters and electroencephalogram (EEG) power spectra. The main findings preceding migraine attacks were a significant decrease in the number of arousals, a decrease in rapid eye movement (REM) density, a significant decrease of beta power in the slow wave sleep, and a decrease of alpha power during the first REM period. The results suggest a decrease in cortical activation during sleep preceding migraine attacks. According to the models of sleep regulation, alterations in the function of aminergic or cholinergic brainstem nuclei have to be discussed.

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GENETIC REGULATION OF MUP PRODUCTION IN RECOMBINANT INBRED MICE

Genetics ◽

10.1093/genetics/93.1.173 ◽

1979 ◽

Vol 93 (1) ◽

pp. 173-181

Author(s):

P R Szoka ◽

K Paigen

Keyword(s):

Recombinant Inbred ◽

Inbred Mice ◽

Genetic Regulation ◽

Inbred Strains ◽

Urinary Proteins ◽

Major Urinary Proteins ◽

Recombinant Inbred Mice ◽

Inbred Strains Of Mice ◽

Before And After ◽

Kinetics Of

ABSTRACT Inbred strains of mice excrete all three major urinary proteins (mups) when induced by testosterone, but differ as to the relative proportions and total levels of each mup present. We have now determined the urinary mup pheno-types before and after testosterone treatment of seven recombinant inbred strains derived from progenitor strains exhibiting different mup phenotypes. The results codirm previous observations indicating that total control of mup protein production is a multigenic process. One locus, Mup-a on chromosome 4, determines the relative mup protein proportions after induction by testoster- one. Mup-a, together with other genetic sites, determines the basal mup proportions. Genes other than Mup-a determine the kinetics of mup induction and total mup excretion.

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Impact of pulmonary rehabilitation in sleep in COPD patients measured by actigraphy

PLoS ONE ◽

10.1371/journal.pone.0248466 ◽

2021 ◽

Vol 16 (3) ◽

pp. e0248466

Author(s):

Suman B. Thapamagar ◽

Kathleen Ellstrom ◽

James D. Anholm ◽

Ramiz A. Fargo ◽

Nagamani Dandamudi

Keyword(s):

Sleep Quality ◽

Pulmonary Rehabilitation ◽

Sleep Onset ◽

Poor Sleep ◽

Poor Sleep Quality ◽

Subjective Sleep Quality ◽

Subjective Sleep ◽

Copd Patients ◽

Before And After ◽

Sleep Parameters

Introduction Chronic obstructive pulmonary disease (COPD) patients have poor sleep quality, longer time to sleep onset and frequent nocturnal awakenings. Poor sleep quality in COPD is associated with poor quality of life (QoL), increased exacerbations and increased mortality. Pulmonary rehabilitation (PR) improves functional status and QoL in COPD but effects on sleep are unclear. PR improves subjective sleep quality but there is paucity of objective actigraphy data. We hypothesized that actigraphy would demonstrate subjective and objective improvement in sleep following PR. Paired comparisons (t-test or Wilcoxon-signed-rank test) were performed before and after PR data on all variables. Methods This retrospective study of COPD patients undergoing PR utilized actigraphy watch recordings before and after 8-weeks of PR to assess changes in sleep variables including total time in bed (TBT), total sleep time (TST), sleep onset latency (SOL), sleep efficiency (SE), wakefulness after sleep onset (WASO) and total nocturnal awakenings. A change in Pittsburg Sleep Quality Index (PSQI) was a secondary outcome. PSQI was performed before and after PR. Results Sixty-nine patients were included in the final analysis. Most participants were male (97%), non-obese (median BMI 27.5, IQR 24.3 to 32.4 kg/m2) with an average age of 69 ± 8 years and 71% had severe COPD (GOLD stage 3 or 4). Prevalence of poor sleep quality (PSQI ≥5) was 86%. Paired comparisons did not show improvement in actigraphic sleep parameters following 8-weeks PR despite improvements in 6-min-walk distance (6MWD, mean improvement 54 m, 95% CI 34 m to 74 m, p<0.0001) and St. George’s Respiratory Questionnaire scores (SGRQ, mean improvement 7.7 points, 95% CI 5.2 to 10.2, p<0.0001). Stratified analysis of all sleep variables by severity of COPD, BMI, mood, mental status, 6-MWD and SGRQ did not show significant improvement after PR. In Veterans with poor sleep quality (PSQI ≥ 5), PR improved subjective sleep quality (PSQI, mean difference 0.79, 95% CI 0.07 to 1.40, p = 0.03). Conclusions Pulmonary rehabilitation improved subjective sleep quality in Veterans who had poor sleep quality at the beginning of the PR but did not improve objective sleep parameters by actigraphy. Our findings highlight the complex interactions among COPD, sleep and exercise.

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Physical Activity and Inhibitory Control: The Mediating Role of Sleep Quality and Sleep Efficiency

Brain Sciences ◽

10.3390/brainsci11050664 ◽

2021 ◽

Vol 11 (5) ◽

pp. 664

Author(s):

Lin Li ◽

Qian Yu ◽

Wenrui Zhao ◽

Fabian Herold ◽

Boris Cheval ◽

...

Keyword(s):

Physical Activity ◽

Sleep Quality ◽

Inhibitory Control ◽

Sleep Efficiency ◽

Equation Modeling ◽

Control Performance ◽

Subjective Sleep Quality ◽

Subjective Sleep ◽

Sleep Parameters ◽

The Relationship

Objectives: the current study aimed to investigate the relationship between physical activity (PA) level and inhibitory control performance and then to determine whether this association was mediated by multiple sleep parameters (i.e., subjective sleep quality, sleep duration, sleep efficiency, and sleep disturbance). Methods: 180 healthy university students (age: 20.15 ± 1.92 years) from the East China Normal University were recruited for the present study. PA level, sleep parameters, and inhibitory control performance were assessed using the International Physical Activity Questionnaire (IPAQ), the Pittsburgh Sleep Quality Index Scale (PSQI), and a Stroop test, respectively. The data were analyzed using structural equation modeling. Results: A higher level of PA was linked to better cognitive performance. Furthermore, higher subjective sleep quality and sleep efficiency were associated with better inhibitory control performance. The mediation analysis revealed that subjective sleep quality and sleep efficiency mediated the relationship between PA level and inhibitory control performance. Conclusion: our results are in accordance with the literature and buttress the idea that a healthy lifestyle that involves a relatively high level of regular PA and adequate sleep patterns is beneficial for cognition (e.g., inhibitory control performance). Furthermore, our study adds to the literature that sleep quality and sleep efficiency mediates the relationship between PA and inhibitory control performance, expanding our knowledge in the field of exercise cognition.

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Thermal Comfort and Sleep Quality of Indonesian Students Living in Japan during Summer and Winter

Buildings ◽

10.3390/buildings11080326 ◽

2021 ◽

Vol 11 (8) ◽

pp. 326

Author(s):

Wiwik Budiawan ◽

Kazuyo Tsuzuki

Keyword(s):

Sleep Quality ◽

Thermal Comfort ◽

Thermal Sensation ◽

Thermal Conditions ◽

Physical Conditions ◽

Subjective Sleepiness ◽

Adaptive Action ◽

Quality Sleep ◽

Sleep Parameters

Thermal comfort is crucial in satisfaction and maintaining quality sleep for occupants. In this study, we investigated the comfort temperature in the bedroom at night and sleep quality for Indonesian students during summer and winter. Eighteen male Indonesian students aged 29 ± 4 years participated in this study. The participants had stayed in Japan for about six months. We evaluated the sleep parameters using actigraphy performed during summer and winter. All participants completed the survey regarding thermal sensation, physical conditions, and subjective sleepiness before sleep. The temperature and relative humidity of participants’ bedrooms were also measured. We found that the duration on the bed during winter was significantly longer than that during summer. However, sleeping efficiency during winter was significantly worse than that during summer. The bedroom temperature of the participants was in the range of comfort temperature in Indonesia. With the average bedroom air temperature of 22.2 °C, most of the participants still preferred “warm” and felt “slightly comfortable” during winter. The average comfort temperature each season calculated using the Griffiths method was 28.1 °C during summer and 23.5 °C during winter. In conclusion, differences in adaptive action affect bedroom thermal conditions. Furthermore, habits encourage the sleep performance of Indonesian students.

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Bi-Temporal Anodal Transcranial Direct Current Stimulation during Slow-Wave Sleep Boosts Slow-Wave Density but Not Memory Consolidation

Brain Sciences ◽

10.3390/brainsci11040410 ◽

2021 ◽

Vol 11 (4) ◽

pp. 410

Author(s):

Simon Ruch ◽

Kristoffer Fehér ◽

Stephanie Homan ◽

Yosuke Morishima ◽

Sarah Maria Mueller ◽

...

Keyword(s):

Episodic Memory ◽

Slow Wave ◽

Direct Current ◽

Memory Consolidation ◽

Transcranial Direct Current Stimulation ◽

Slow Wave Sleep ◽

Memory Performance ◽

Direct Current Stimulation ◽

Sleep Parameters ◽

Episodic Memories

Slow-wave sleep (SWS) has been shown to promote long-term consolidation of episodic memories in hippocampo–neocortical networks. Previous research has aimed to modulate cortical sleep slow-waves and spindles to facilitate episodic memory consolidation. Here, we instead aimed to modulate hippocampal activity during slow-wave sleep using transcranial direct current stimulation in 18 healthy humans. A pair-associate episodic memory task was used to evaluate sleep-dependent memory consolidation with face–occupation stimuli. Pre- and post-nap retrieval was assessed as a measure of memory performance. Anodal stimulation with 2 mA was applied bilaterally over the lateral temporal cortex, motivated by its particularly extensive connections to the hippocampus. The participants slept in a magnetic resonance (MR)-simulator during the recordings to test the feasibility for a future MR-study. We used a sham-controlled, double-blind, counterbalanced randomized, within-subject crossover design. We show that stimulation vs. sham significantly increased slow-wave density and the temporal coupling of fast spindles and slow-waves. While retention of episodic memories across sleep was not affected across the entire sample of participants, it was impaired in participants with below-average pre-sleep memory performance. Hence, bi-temporal anodal direct current stimulation applied during sleep enhanced sleep parameters that are typically involved in memory consolidation, but it failed to improve memory consolidation and even tended to impair consolidation in poor learners. These findings suggest that artificially enhancing memory-related sleep parameters to improve memory consolidation can actually backfire in those participants who are in most need of memory improvement.

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A pilot study on essential oil aroma stimulation for enhancing slow-wave EEG in sleeping brain

Scientific Reports ◽

10.1038/s41598-020-80171-x ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Li-Wei Ko ◽

Cheng-Hua Su ◽

Meng-Hsun Yang ◽

Shen-Yi Liu ◽

Tung-Ping Su

Keyword(s):

Essential Oil ◽

Sleep Quality ◽

Slow Wave ◽

Slow Wave Sleep ◽

Sleep Eeg ◽

Poor Sleep ◽

Alpha Power ◽

Poor Sleep Quality ◽

Healthy Human ◽

Study Results

AbstractSleep quality is important to health and life quality. Lack of sleep can lead to a variety of health issues and reduce in daytime function. Recent study by Fultz et al. also indicated that sleep is crucial to brain metabolism. Delta power in sleep EEG often indicates good sleep quality while alpha power usually indicates sleep interruptions and poor sleep quality. Essential oil has been speculated to improve sleep quality. Previous studies also suggest essential oil aroma may affect human brain activity when applied awake. However, those studies were often not blinded, which makes the effectiveness and mechanism of aroma a heavily debated topic. In this study, we aim to explore the effect of essential oil aroma on human sleep quality and sleep EEG in a single-blinded setup. The aroma was released when the participants are asleep, which kept the influence of psychological expectation to the minimum. We recruited nine young, healthy participants with regular lifestyle and no sleep problem. All participants reported better sleep quality and more daytime vigorous after exposing to lavender aroma in sleep. We also observed that upon lavender aroma releases, alpha wave in wake stage was reduced while delta wave in slow-wave sleep (SWS) was increased. Lastly, we found that lavender oil promote occurrence of SWS. Overall, our study results show that essential oil aroma can be used to promote both subjective and objective sleep quality in healthy human subjects. This makes aroma intervention a potential solution for poor sleep quality and insomnia.

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