Endurance training-induced changes in alkali light chain patterns in type IIB fibers of the rat

2003 ◽  
Vol 94 (3) ◽  
pp. 923-929 ◽  
Author(s):  
Masanobu Wada ◽  
Shuichiro Inashima ◽  
Takashi Yamada ◽  
Satoshi Matsunaga
Keyword(s):  
Endurance Training ◽  
Light Chain ◽  
Myosin Light Chain ◽  
Fiber Type ◽  
Vastus Lateralis ◽  
Vastus Lateralis Muscle ◽  
Lateralis Muscle ◽  
Induced Changes ◽  
Whole Muscle ◽  
Dimensional Electrophoresis

The effects of endurance training on the expression of myosin were electrophoretically analyzed in the deep portion of vastus lateralis muscle from the rat. A 10-wk running program led to increases ( P < 0.01) in myosin heavy chain (MHC) 2a and 2d with a decrease ( P < 0.01) in MHC2b. Training also evoked a rearrangement of the isomyosin pattern with decreases in fast isomyosin (FM) 1 ( P < 0.01) and FM2 ( P < 0.05) and a rise in intermediate isomyosin ( P < 0.01). These changes were accompanied by a 61% decrease ( P < 0.01) in myosin light chain (MLC) 3F (11.8 ± 2.7 vs. 4.6 ± 4.2%). Two-dimensional electrophoresis made it possible to separate the triplet of isomyosins (FMb) consisting of MHC2b. Training elicited a 26% decrease ( P < 0.05) in the FM1b fraction within FMb, i.e., FM1b/(FM1b + FM2b + FM3b) (24.2 ± 5.5 vs. 18.0 ± 4.3%). These changes resulted in a 10% decrease ( P < 0.05) in the MLC3Ffraction, i.e., MLC3F/(MLC1F + MLC3F), in FMb (44.9 ± 4.5 vs. 40.3 ± 3.2%). These results suggest that endurance training may exert the depressive effect on the contractile velocity of type IIB fibers and that a training-induced decrease in the contractile velocity of whole muscle may be caused by alterations in fast alkali MLC complements within a given fiber type as well as by transitions in MHC-based fiber populations.

Halothane Induces Calcium Release from Human Skinned Masseter Muscle Fibers 

1999 ◽  
Vol 90 (4) ◽  
pp. 1019-1025 ◽  
Author(s):  
Hugo Reyford ◽  
Pascal J. Adnet ◽  
Benoit Tavernier ◽  
Sebastien Beague ◽  
Joel Ferri ◽  
...  
Keyword(s):  
Sarcoplasmic Reticulum ◽  
Masseter Muscle ◽  
Muscle Tone ◽  
Fiber Type ◽  
Vastus Lateralis ◽  
Vastus Lateralis Muscle ◽  
Significant Difference ◽  
Lateralis Muscle ◽  
Healthy Persons

Background An increase in masseter muscle tone in response to halothane or succinylcholine anesthesia (or both) can be observed in healthy persons. Thus the authors compared the fiber-type halothane and succinylcholine sensitivities in human masseter and vastus lateralis muscles. Methods Masseter and vastus lateralis muscle segments were obtained from 13 and 9 healthy persons, respectively. After chemical skinning of a single fiber and loading the sarcoplasmic reticulum with Ca++ 0.16 microM solution, halothane (0.5-4 vol% bubbled in the incubating solution), succinylcholine (0.1 microM to 10 mM), or both sensitivities were defined as the concentration inducing more than 10% of the maximum tension obtained by application of 16 microM Ca++ solution. The myofilament response to Ca++ was studied with and without halothane by observing the isometric tension of skinned masseter fibers challenged with increasing concentrations of Ca++. Muscle fiber type was determined by the difference in strontium-induced tension measurements. Results A significant difference in halothane sensitivity was found between type 1 masseter fibers (0.6+/-0.2 vol%; mean +/- SD) versus type 1 (2.7+/-0.6 vol%) and type 2 vastus lateralis muscle (2.5+/-0.4 vol%). Succinylcholine did not induce Ca++ release by the sarcoplasmic reticulum. In the masseter muscle, 0.75 vol% halothane decreased the maximal activated tension by 40% but did not change the Ca++ concentration that yields 50% of the maximal tension. Conclusions The very low halothane threshold for Ca++ release from the masseter muscle usually could be counteracted by a direct negative inotropic effect on contractile proteins. However, halothane may increase the sensitivity of the sarcoplasmic reticulum Ca++ release to succinylcholine-induced depolarization, leading to an increase in masseter muscle tone.

scholarly journals Vastus lateralis muscle architecture to estimate knee extension moment of older individuals

2015 ◽  
Vol 21 (4) ◽  
pp. 428-435
Author(s):  
Guilherme Auler Brodt ◽  
Jeam Marcel Geremia ◽  
Mônica de Oliveira Melo ◽  
Marco Aurélio Vaz ◽  
Jefferson Fagundes Loss
Keyword(s):  
Fiber Type ◽  
Vastus Lateralis ◽  
Biomechanical Model ◽  
Knee Extension ◽  
Muscle Architecture ◽  
Vastus Lateralis Muscle ◽  
Older Individuals ◽  
Lateralis Muscle ◽  
Muscle Moments ◽  
The Individual

Abstract The aim of this study was to compare the knee extension moment of older individuals with the muscle moment estimated through a biomechanical model. This was accomplished by using (1) the specific muscle architecture data of individuals, and (2) the generic muscle architecture available in the literature. The muscle force estimate was determined using a model with the muscle architecture from cadavers and the individual vastus lateralis muscle architecture of sixteen older volunteers. For the muscle moment comparison, all of the volunteers performed maximal voluntary isometric contractions (MVIC) in five different knee extension position angles. The architectural data was acquired using both resonance and ultrasound imaging. Both estimated muscle moments (generic and individual) were higher than the experimental. The architecture of the other vastii may be necessary to make the model more accurate for the older population. Although other factors inherent to ageing, such as co-contractions, fiber type percentage, and passive forces are not considered in the model, they could be responsible for the differences between moments in older people.

Fiber Type Composition of the Vastus Lateralis Muscle of Young Men and Women

2000 ◽  
Vol 48 (5) ◽  
pp. 623-629 ◽  
Author(s):  
Robert S. Staron ◽  
Fredrick C. Hagerman ◽  
Robert S. Hikida ◽  
Thomas F. Murray ◽  
David P. Hostler ◽  
...  
Keyword(s):  
Fiber Type ◽  
Vastus Lateralis ◽  
Young Men ◽  
Vastus Lateralis Muscle ◽  
Fiber Types ◽  
Cross Sectional ◽  
Men And Women ◽  
Type Composition ◽  
Lateralis Muscle ◽  
Fiber Type Distribution

SUMMARY This study presents data collected over the past 10 years on the muscle fiber type composition of the vastus lateralis muscle of young men and women. Biopsies were taken from the vastus lateralis muscle of 55 women (21.2 ± 2.2 yr) and 95 men (21.5 ± 2.4 yr) who had volunteered to participate in various research projects. Six fiber types (I, IC, IIC, IIA, IIAB, and IIB) were classified using mATPase histochemistry, and cross-sectional area was measured for the major fiber types (I, IIA, and IIB). Myosin heavy chain (MHC) content was determined electrophoretically on all of the samples from the men and on 26 samples from the women. With the exception of fiber Type IC, no significant differences were found between men and women for muscle fiber type distribution. The vastus lateralis muscle of both the men and women contained approximately 41% I, 1% IC, 1% IIC, 31% IIA, 6% IIAB, and 20% IIB. However, the cross-sectional area of all three major fiber types was larger for the men compared to the women. In addition, the Type IIA fibers were the largest for the men, whereas the Type I fibers tended to be the largest for the women. Therefore, gender differences were found with regard to the area occupied by each specific fiber type: IIA>I>IIB for the men and I>IIA>IIB for the women. These data establish normative values for the mATPase-based fiber type distribution and sizes in untrained young men and women.

Myosin light chain phosphorylation and contractile performance of human skeletal muscle

1988 ◽  
Vol 66 (1) ◽  
pp. 49-54 ◽  
Author(s):  
D. S. Stuart ◽  
M. D. Lingley ◽  
R. W. Grange ◽  
M. E. Houston
Keyword(s):  
Light Chain ◽  
Myosin Light Chain ◽  
Vastus Lateralis ◽  
Muscle Length ◽  
Light Chains ◽  
Muscle Fibres ◽  
Vastus Lateralis Muscle ◽  
Type Ii ◽  
Phosphate Content ◽  
Contractile Performance

Twitch tension and maximal unloaded velocity of human knee extensor muscles were studied under conditions of low phosphate content of the phosphorylatable light chains (P-light chains) of myosin and elevated phosphate content, following a 10-s maximal voluntary isometric contraction (MVC). After the MVC, twitch tension was significantly potentiated, with greater potentiation observed at a shorter muscle length (p < 0.05). The MVC was associated with at least a twofold increase in phosphate content of the fast (LC2F) and two slow (LC2S and LC2S′) P-light chains, but this increase was unrelated to muscle length. No significant differences in knee extension velocity were observed between conditions where P-light chains had low or elevated phosphate content. Positive but nonsignificant correlations were noted between the extent of twitch potentiation and phosphate content of individual P-light chains as well as the percentage of type II muscle fibres in vastus lateralis muscle. No significant relationships were determined for myosin light chain kinase activity and either P-light chain phosphorylation or type II fibre percentage. These data suggest that, unlike other mammalian fast muscles, P-light chain phosphorylation of mixed human muscles is not strongly associated with altered contractile performance.

The Effect of Aerobic Exercise Training on the Distribution of Succinate Dehydrogenase Activity Throughout Muscle Fibres

1998 ◽  
Vol 23 (1) ◽  
pp. 74-86 ◽  
Author(s):  
Philip D. Chilibeck ◽  
Gordon J. Bell ◽  
Teresa Socha ◽  
Tom Martin
Keyword(s):  
Endurance Training ◽  
Succinate Dehydrogenase ◽  
Vastus Lateralis ◽  
Aerobic Exercise Training ◽  
Control Group ◽  
Muscle Fibres ◽  
Vastus Lateralis Muscle ◽  
Type I ◽  
Lateralis Muscle ◽  
Subsarcolemmal Mitochondria

We evaluated the effect of endurance training (cycling 3 times per week for 12 weeks) on succinate dehydrogenase (SDH) activity in the subsarcolemmal (SS) and intermyofibrillar (IMF) regions of vastus lateralis muscle fibres in 7 individuals (4 females and 3 males). SDH activity of the SS region increased 9.4% and 12.8% in type I and II fibres, respectively (p < .05). SDH activity of the IMF region increased 4.7% and 6.7% in type I and II fibres, respectively (p < .05). This was less than the increase in the SS region (p < .O5). No significant changes were observed in a control group (4 females and 3 males). These data suggest that mitochondria in the SS and IMF regions of human vastus lateralis muscle fibres are sensitive to endurance training. The greater response in the SS region suggests that the metabolic requirements of SS mitochondria were stressed to a greater extent than IMF mitochondria with endurance training. Key words: subsarcolemmal mitochondria, intermyofibrillar mitochondria

FIBER TYPE COMPOSITION OF THE VASTUS LATERALIS MUSCLE OF YOUNG MEN AND WOMEN

1999 ◽  
Vol 31 (Supplement) ◽  
pp. S328
Author(s):  
M. T. Crill ◽  
R. S. Staron ◽  
F. C. Hagerman ◽  
R. S. Hikida ◽  
D. Hostler ◽  
...  
Keyword(s):  
Fiber Type ◽  
Vastus Lateralis ◽  
Young Men ◽  
Vastus Lateralis Muscle ◽  
Men And Women ◽  
Fiber Type Composition ◽  
Type Composition ◽  
Lateralis Muscle

EFFECT OF ACTIVITIES OF DAILY LIVING ON FIBER TYPE ATROPHY OF THE VASTUS LATERALIS MUSCLE IN PATIENTS WITH JOINT DISORDERS

1998 ◽  
Vol 77 (2) ◽  
pp. 122-127 ◽  
Author(s):  
Shoichi Tanaka ◽  
Kenji Hachisuka ◽  
Soichiro Nara ◽  
Hajime Ogata ◽  
Yasuyuki Kobayashi ◽  
...  
Keyword(s):  
Activities Of Daily Living ◽  
Daily Living ◽  
Fiber Type ◽  
Vastus Lateralis ◽  
Vastus Lateralis Muscle ◽  
Lateralis Muscle

Influence of complete spinal cord injury on skeletal muscle within 6 mo of injury

1999 ◽  
Vol 86 (1) ◽  
pp. 350-358 ◽  
Author(s):  
Michael J. Castro ◽  
David F. Apple ◽  
Robert S. Staron ◽  
Gerson E. R. Campos ◽  
Gary A. Dudley
Keyword(s):  
Skeletal Muscle ◽  
Fiber Type ◽  
Vastus Lateralis ◽  
Vastus Lateralis Muscle ◽  
Type I ◽  
Cord Injury ◽  
Lateralis Muscle ◽  
Type I Fibers ◽  
Femoris Muscle ◽  
Over Time

This study examined the influence of spinal cord injury (SCI) on affected skeletal muscle. The right vastus lateralis muscle was biopsied in 12 patients as soon as they were clinically stable (average 6 wk after SCI), and 11 and 24 wk after injury. Samples were also taken from nine able-bodied controls at two time points 18 wk apart. Surface electrical stimulation (ES) was applied to the left quadriceps femoris muscle to assess fatigue at these same time intervals. Biopsies were analyzed for fiber type percent and cross-sectional area (CSA), fiber type-specific succinic dehydrogenase (SDH) and α-glycerophosphate dehydrogenase (GPDH) activities, and myosin heavy chain percent. Controls showed no change in any variable over time. Patients showed 27–56% atrophy ( P = 0.000) of type I, IIa, and IIax+IIx fibers from 6 to 24 wk after injury, resulting in fiber CSA approximately one-third that of controls. Their fiber type specific SDH and GPDH activities increased ( P ≤ 0.001) from 32 to 90% over the 18 wk, thereby approaching or surpassing control values. The relative CSA of type I fibers and percentage of myosin heavy chain type I did not change. There was apparent conversion among type II fiber subtypes; type IIa decreased and type IIax+IIx increased ( P ≤ 0.012). Force loss during ES did not change over time for either group but was greater ( P = 0.000) for SCI patients than for controls overall (27 vs. 9%). The results indicate that vastus lateralis muscle shows marked fiber atrophy, no change in the proportion of type I fibers, and a relative independence of metabolic enzyme levels from activation during the first 24 wk after clinically complete SCI. Over this time, quadriceps femoris muscle showed moderately greater force loss during ES in patients than in controls. It is suggested that the predominant response of mixed human skeletal muscle within 6 mo of SCI is loss of contractile protein. Therapeutic interventions could take advantage of this to increase muscle mass.

scholarly journals Enhancement of microvessel tortuosity in the vastus lateralis muscle of old men in response to endurance training

2004 ◽  
Vol 554 (2) ◽  
pp. 559-569 ◽  
Author(s):  
N. Charifi ◽  
F. Kadi ◽  
L. Féasson ◽  
F. Costes ◽  
A. Geyssant ◽  
...  
Keyword(s):  
Endurance Training ◽  
Vastus Lateralis ◽  
Vastus Lateralis Muscle ◽  
Lateralis Muscle ◽  
Old Men
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