Natural antisense transcripts: sound or silence?

2005 ◽  
Vol 23 (2) ◽  
pp. 125-131 ◽  
Author(s):  
Andreas Werner ◽  
Ariane Berdal

Antisense RNA was a rather uncommon term in a physiology environment until short interfering RNAs emerged as the tool of choice to knock down the expression of specific genes. As a consequence, the concept of RNA having regulatory potential became widely accepted. Yet, there is more to come. Computational studies suggest that between 15 and 25% of mammalian genes overlap, giving rise to pairs of sense and antisense RNAs. The resulting transcripts potentially interfere with each other’s processing, thus representing examples of RNA-mediated gene regulation by endogenous, naturally occurring antisense transcripts. Concerns that the large-scale antisense transcription may represent transcriptional noise rather than a gene regulatory mechanism are strongly opposed by recent reports. A relatively small, well-defined group of antisense or noncoding transcripts is linked to monoallelic gene expression as observed in genomic imprinting, X chromosome inactivation, and clonal expression of B and T leukocytes. For the remaining, much larger group of bidirectionally transcribed genes, however, the physiological consequences of antisense transcription as well as the cellular mechanism(s) involved remain largely speculative.

Genetics ◽  
2002 ◽  
Vol 160 (3) ◽  
pp. 1051-1065
Author(s):  
Claudia B Zraly ◽  
Yun Feng ◽  
Andrew K Dingwall

Abstract We identified and characterized the Drosophila gene ear (ENL/AF9-related), which is closely related to mammalian genes that have been implicated in the onset of acute lymphoblastic and myelogenous leukemias when their products are fused as chimeras with those of human HRX, a homolog of Drosophila trithorax. The ear gene product is present in all early embryonic cells, but becomes restricted to specific tissues in late embryogenesis. We mapped the ear gene to cytological region 88E11-13, near easter, and showed that it is deleted by Df(3R)ea5022rx1, a small, cytologically invisible deletion. Annotation of the completed Drosophila genome sequence suggests that this region might contain as many as 26 genes, most of which, including ear, are not represented by mutant alleles. We carried out a large-scale noncom-plementation screen using Df(3R)ea5022rx1 and chemical (EMS) mutagenesis from which we identified sevenc novel multi-allele recessive lethal complementation groups in this region. An overlapping deficiency, Df(3R)Po4, allowed us to map several of these groups to either the proximal or the distal regions of Df(3R)ea5022rx1. One of these complementation groups likely corresponds to the ear gene as judged by map location, terminal phenotype, and reduction of EAR protein levels.


Fitoterapia ◽  
2021 ◽  
pp. 104909
Author(s):  
Yuan Xiong ◽  
Guang-Hao Zhu ◽  
Hao-Nan Wang ◽  
Qing Hu ◽  
Li-Li Chen ◽  
...  

Immunobiology ◽  
2019 ◽  
Vol 224 (4) ◽  
pp. 560-564 ◽  
Author(s):  
Mona Patoughi ◽  
Soudeh Ghafouri-Fard ◽  
Shahram Arsang-Jang ◽  
Mohammad Taheri

1998 ◽  
Vol 41 (4) ◽  
pp. 1161-1172 ◽  
Author(s):  
JOEL F. HARRINGTON

Gender relations in German history: power, agency, and experience from the sixteenth to the twentieth century. Edited by Lynn Abrams and Elizabeth Harvey. London: UCL, 1996. Pp. x+262. ISBN 1-85728-485-2. £12.95.Adultery and divorce in Calvin's Geneva. By Robert M. Kingdon. Cambridge, Mass., and London: Harvard UP, 1995. Pp. ix+214. ISBN 0-674-00520-1 (hb). £18.50.Housecraft and statecraft: domestic service in Renaissance Venice, 1400–1600. By Dennis Romano. Baltimore: Johns Hopkins University Press, 1996. Pp. xxvi+333. ISBN 0-8018-5288-9. £37.00.The European nobility, 1400–1800. By Jonathan Dewald. New approaches to European history, ix. Cambridge: Cambridge University Press, 1996. Pp. xvii+209. ISBN 0-521-42528-x (pb). £12.95.Garden and grove: the Italian Renaissance garden in the English imagination, 1600–1750. By John Dixon Hunt. Philadelphia: University of Pennsylvania, 1996. Pp. xix+268. ISBN 0-8122-1604-0 (pb). £23.50.Like an ancient woodsman or a guide through the Amazonian jungle, the ideal historian possesses at least two kinds of expertise: enough familiarity with the general terrain to plan successful expeditions and enough experience in the field to make inevitable adjustments to ‘the big picture’ when underway. Of course in the real world (of both geography and history) the tasks of exploration and cartography are often bifurcated, without necessarily disastrous results. The historian who is equally skilled at both close-up description and large-scale theorizing is consequently celebrated as a rare and valued anomaly. Meanwhile, for most of us stumbling scouts, the world beyond our familiar trails remains largely one of learned lore, with connections to our own limited forays often vague at best. Unless, of course, we are fortunate enough to come across something which provides an almost magical link between the narrow and the wide, the micro and the macro.


2016 ◽  
Vol 12 (2) ◽  
pp. 588-597 ◽  
Author(s):  
Jun Wu ◽  
Xiaodong Zhao ◽  
Zongli Lin ◽  
Zhifeng Shao

Transcriptional regulation is a basis of many crucial molecular processes and an accurate inference of the gene regulatory network is a helpful and essential task to understand cell functions and gain insights into biological processes of interest in systems biology.


2010 ◽  
Vol 54 (10) ◽  
pp. 4049-4058 ◽  
Author(s):  
Brandon Findlay ◽  
George G. Zhanel ◽  
Frank Schweizer

ABSTRACT Naturally occurring cationic antimicrobial peptides (AMPs) and their mimics form a diverse class of antibacterial agents currently validated in preclinical and clinical settings for the treatment of infections caused by antimicrobial-resistant bacteria. Numerous studies with linear, cyclic, and diastereomeric AMPs have strongly supported the hypothesis that their physicochemical properties, rather than any specific amino acid sequence, are responsible for their microbiological activities. It is generally believed that the amphiphilic topology is essential for insertion into and disruption of the cytoplasmic membrane. In particular, the ability to rapidly kill bacteria and the relative difficulty with which bacteria develop resistance make AMPs and their mimics attractive targets for drug development. However, the therapeutic use of naturally occurring AMPs is hampered by the high manufacturing costs, poor pharmacokinetic properties, and low bacteriological efficacy in animal models. In order to overcome these problems, a variety of novel and structurally diverse cationic amphiphiles that mimic the amphiphilic topology of AMPs have recently appeared. Many of these compounds exhibit superior pharmacokinetic properties and reduced in vitro toxicity while retaining potent antibacterial activity against resistant and nonresistant bacteria. In summary, cationic amphiphiles promise to provide a new and rich source of diverse antibacterial lead structures in the years to come.


2021 ◽  
pp. 1-8
Author(s):  
Pillkyu HWANG ◽  
Yae-Ahn PARK

On 23 July 2018, when the villagers gathered around the porch to wrap up the day with a good chat, one of the five auxiliary dams of the Xe-Pian Xe-Namnoy hydropower dam in Attapeu province, the southeastern state of Laos, collapsed. Four days before the collapse, reports of cracks and subsidence started to come through. It should have been enough to prompt evacuation warning issuance by the Xe-Pian Xe-Namnoy Power Co. Ltd (PNPC), a consortium of South Korean companies SK Engineering and Construction (SK E&C) and Korea Western Power Company (KOWEPO), Thailand-based RATCH Group, and Lao Holding State Enterprise (LHSE). PNPC has a Concession Agreement with the Laos government ‘to plan, design, finance, construct, own, operate and maintain’ the Xe-Pian Xe-Namnoy hydropower dam. The warning was issued, but it came too late.


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