scholarly journals Laboratory Diagnosis ofChlamydia PneumoniaeInfections

1995 ◽  
Vol 6 (4) ◽  
pp. 198-203 ◽  
Author(s):  
Rosanna W Peeling
Keyword(s):  
Chlamydia Pneumoniae ◽  
Fluorescent Antibody ◽  
Respiratory Illness ◽  
Laboratory Diagnosis ◽  
Turnaround Time ◽  
Diagnostic Methods ◽  
Detection Methods ◽  
Direct Fluorescent Antibody ◽  
Polymerase Chain ◽  
Rapid Laboratory

Chlamydia pneumoniaeis an important cause of respiratory illness. There is a need for accurate and rapid laboratory diagnostic methods that will lead to improved patient care, appropriate use of antimicrobial therapy and a better understanding of the epidemiology of this emerging pathogen. Culture is highly specific but is technically demanding, expensive, has a long turnaround time and its sensitivity is highly dependent on transport conditions. Antigen detection tests such as enzyme immunoassay and direct fluorescent antibody assay, and molecular detection methods such as the polymerase chain reaction assay, may provide a rapid diagnosis without the requirement for stringent transport conditions. The results of these tests should be interpreted with caution until more thorough evaluation is available. Serology remains the method of choice. The limitations of different serological methods for the laboratory diagnosis ofC pneumoniaeare discussed.

scholarly journals A Comparative Analysis of Polymerase Chain Reaction and Direct Fluorescent Antibody Test for Diagnosis of Genital Herpes

2017 ◽  
Vol 9 (01) ◽  
pp. 053-056 ◽  
Author(s):  
Vrushali Patwardhan ◽  
Preena Bhalla ◽  
Deepti Rawat ◽  
Vijay Kumar Garg ◽  
Kabir Sardana ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Genital Herpes ◽  
Fluorescent Antibody ◽  
Antibody Test ◽  
Conventional Polymerase Chain Reaction ◽  
Genital Ulcer ◽  
Chain Reaction ◽  
Direct Fluorescent Antibody ◽  
Polymerase Chain ◽  
Simplex Virus

ABSTRACT Objective: To compare laboratory tests that can simultaneously detect and type herpes simplex virus (HSV) directly from the genital ulcer specimens in clinically suspected cases of genital herpes. Materials and Methods: A study was conducted over 10 months and 44 adult male and female patients clinically suspected with genital herpes were recruited. Genital ulcer swab specimens were subjected to glycoprotein-G gene-based conventional polymerase chain reaction (PCR) and commercially available direct fluorescent antibody (DFA) test and the results were compared. Results: PCR for HSV was positive in 82% (36/44) cases. DFA was positive in 68.2% (30/44) cases. There was 100% agreement between HSV types detected by DFA and PCR. The strength of agreement between the results was better in primary genital herpes than recurrent cases. Conclusion: PCR was found to be better in the detection of HSV in recurrent genital herpes patients. It is a better modality, especially when genital herpes clinically presents with ulcerative or crusted lesions, and is also a cheaper alternative as compared to DFA.

scholarly journals Comparative assessment of seller’s staining test (SST) and direct fluorescent antibody test for rapid and accurate laboratory diagnosis of rabies

2016 ◽  
Vol 16 (1) ◽  
pp. 123 ◽  
Author(s):  
Ishaya S. Tekki ◽  
Zhakum N. Ponfa ◽  
Chika I. Nwosuh ◽  
Peterside R. Kumbish ◽  
Clement L. Jonah ◽  
...  
Keyword(s):  
Fluorescent Antibody ◽  
Antibody Test ◽  
Laboratory Diagnosis ◽  
Comparative Assessment ◽  
Direct Fluorescent Antibody

scholarly journals Evaluation of a Direct Fluorescent Antibody Assay for Detection of Chlamydia pneumoniae

1996 ◽  
Vol 70 (3) ◽  
pp. 224-231 ◽  
Author(s):  
Naoyuki MIYASHITA ◽  
Akira MATSUMOTO ◽  
Rinzo SOEJIMA ◽  
Yoshifumi KUBOTA ◽  
Toshio KISHIMOTO ◽  
...  
Keyword(s):  
Chlamydia Pneumoniae ◽  
Fluorescent Antibody ◽  
Antibody Assay ◽  
Direct Fluorescent Antibody

scholarly journals Nucleic Acid Testing of SARS-CoV-2

2021 ◽  
Vol 22 (11) ◽  
pp. 6150
Author(s):  
Hee-Min Yoo ◽  
Il-Hwan Kim ◽  
Seil Kim
Keyword(s):  
Polymerase Chain Reaction ◽  
Nucleic Acid ◽  
Reverse Transcription ◽  
Diagnostic Methods ◽  
Detection Methods ◽  
Molecular Diagnostic ◽  
Nucleic Acid Testing ◽  
Rt Pcr ◽  
Chain Reaction ◽  
Polymerase Chain

The coronavirus disease 2019 (COVID-19) has caused a large global outbreak. It is accordingly important to develop accurate and rapid diagnostic methods. The polymerase chain reaction (PCR)-based method including reverse transcription-polymerase chain reaction (RT-PCR) is the most widely used assay for the detection of SARS-CoV-2 RNA. Along with the RT-PCR method, digital PCR has emerged as a powerful tool to quantify nucleic acid of the virus with high accuracy and sensitivity. Non-PCR based techniques such as reverse transcription loop-mediated isothermal amplification (RT-LAMP) and reverse transcription recombinase polymerase amplification (RT-RPA) are considered to be rapid and simple nucleic acid detection methods and were reviewed in this paper. Non-conventional molecular diagnostic methods including next-generation sequencing (NGS), CRISPR-based assays and nanotechnology are improving the accuracy and sensitivity of COVID-19 diagnosis. In this review, we also focus on standardization of SARS-CoV-2 nucleic acid testing and the activity of the National Metrology Institutes (NMIs) and highlight resources such as reference materials (RM) that provide the values of specified properties. Finally, we summarize the useful resources for convenient COVID-19 molecular diagnostics.

scholarly journals Bovine rabies incidence in the state of Minas Gerais/Brazil, between 2002 and 2006

2012 ◽  
Vol 79 (2) ◽  
pp. 287-291
Author(s):  
R.S. Ferreira ◽  
R.M.A. de Almeida ◽  
D.A. Nogueira ◽  
N.M.S. Oliveira ◽  
J.E. Fiorini
Keyword(s):  
Nervous Tissue ◽  
Fluorescent Antibody ◽  
Antibody Test ◽  
Minas Gerais ◽  
The State ◽  
Diagnostic Methods ◽  
Inoculation Test ◽  
Tissue Samples ◽  
Direct Fluorescent Antibody ◽  
Vampire Bats

Rabies is an infectious contagious disease of mammals present in all continents except Oceania. It is caused by a Lyssavirus and characterized by acute fatal encephalitis. It is considered endemic in Brazil and transmission to herbivores occurs mainly via vampire bats. The purpose of this study was to evaluate the incidence of bovine rabies in the state of Minas Gerais, as well as the diagnostic methods, applying the time series to analyze trend and seasonality in order to adjust a model to disease in cattle. Between 2002 and 2006, nervous tissue samples were obtained from deceased bovines presenting neurological symptoms and clinically suspected of rabies. The direct fluorescent antibody test (FAT) detected 40.38% (1,293/3,202) positive samples. From all FAT negative tissue samples, 2.67% (51/1,909) tested positive in a mouse inoculation test (MIT). No seasonality was observed and positive cases had a decrease of 0.14 per month.

scholarly journals Hepatitis D: challenges in the estimation of true prevalence and laboratory diagnosis

Gut Pathogens ◽  
2021 ◽  
Vol 13 (1) ◽  
Author(s):  
Lin-Yuan Chen ◽  
Xiao-Yu Pang ◽  
Hemant Goyal ◽  
Rui-Xia Yang ◽  
Hua-Guo Xu
Keyword(s):  
Rna Virus ◽  
Pegylated Interferon ◽  
Laboratory Diagnosis ◽  
International Standards ◽  
Diagnostic Methods ◽  
Detection Methods ◽  
Hepatitis D ◽  
High Risk Sexual Behavior ◽  
Advantages And Disadvantages ◽  
True Prevalence

AbstractHepatitis delta virus (HDV) is a defective single negative chain RNA virus, as its envelope protein synthesis is dependent on hepatitis B virus (HBV). Studies have consistently shown that coinfection of HBV and HDV is the most serious form of viral hepatitis, with accelerated progression to liver cirrhosis and hepatocellular carcinoma. About 74 million of HBV surface antigen (HBsAg) positive patients worldwide are also co-infected with HDV. Besides, patients with intravenous drug use and high-risk sexual behavior are at higher risk of HDV infection. Therapeutic schedules for HDV are limited, and relapse of HDV has been observed after treatment with pegylated interferon alpha. To reduce the transmission of HDV, all people infected with HBV should be screened for HDV. At present, several serological and molecular detection methods are widely used in the diagnosis of HDV. However, due to the lack of international standards diagnostic results from different laboratories are often not comparable. Therefore, the true prevalence of HDV is still unclear. In this manuscript, we have analyzed various factors influencing the estimation of HDV prevalence. We have also discussed about the advantages and disadvantages of currently available HDV laboratory diagnostic methods, in order to provide some ideas for improving the detection of HDV.

Sign in / Sign up

Export Citation Format

Share Document