scholarly journals Expression of Lewisa, Sialyl Lewisa, Lewisx, Sialyl Lewisx, Antigens as Prognostic Factors in Patients with Colorectal Cancer

2000 ◽  
Vol 14 (9) ◽  
pp. 753-760 ◽  
Author(s):  
Tohru Nakagoe ◽  
Kiyoyasu Fukushima ◽  
Atsushi Nanashima ◽  
Terumitsu Sawai ◽  
Takashi Tsuji ◽  
...  
Keyword(s):  
Prognostic Factors ◽  
Colorectal Carcinoma ◽  
Survival Time ◽  
Prognostic Value ◽  
Tumour Progression ◽  
Colorectal Carcinomas ◽  
Altered Expression ◽  
Sialyl Lewis ◽  
Positive Expression ◽  
Cox's Regression

BACKGROUND: Altered expression of blood group-related carbohydrate antigens such as sialyl Lewis (Le)xantigen in tumours is associated with tumour progression behaviour and subsequent prognosis. However, the prognostic value of the expression of Le-related antigens in colorectal tumours remains unclear.PURPOSE: To clarify the prognostic value of Lea, sialyl Lea, Lexand sialyl Lexexpression in colorectal carcinomas as prognostic factors after surgery.PATIENTS AND METHODS: Colorectal carcinoma samples from 101 patients with primary colorectal carcinoma who underwent surgical resection were subject to immunohistochemical analyses for Lea, sialyl Lea, Lexand sialyl Lexexpression with the respective monoclonal antibodies.RESULTS: Lea, sialyl Lea, Lexand sialyl Lexwere expressed in 69 (68.3%), 73 (72.3%), 66 (65.4%) and 76 (75.3%) carcinomas, respectively. The patients with sialyl Lex-expressing tumours had more advanced cancer than those with nonsialyl Lex-expressing tumours (P=0.0029). The survival time after surgery of patients with Lex- or sialyl Lex-expressing tumours was significantly shorter than the survival time of those with non-Lex- or nonsialyl Lex-expressing tumours, respectively (P=0.023 and P=0.0001, respectively). Cox’s regression analysis revealed that Lexand sialyl Lexexpression, separate from stage and histological type, were prognostic variables for patient survival (hazard ratio [HR] for sialyl Lex-positive expression to sialyl Lex-negative expression 2.90; HR for Lex-positive expression to Lex-negative expression 12.76 in stage I/IV, 0.63 in stage II and 1.69 in stage III).CONCLUSIONS: Lexexpression and sialyl Lexexpression in colorectal carcinomas are each associated with poor prognosis. These variables should be considered in the design of future trials.

Comparison of ADAM19 and CUEDC2 expression in EHCC and their clinicopathological significance

2020 ◽  
Vol 14 (16) ◽  
pp. 1573-1584
Author(s):  
Shu Xu ◽  
Shengfu Huang ◽  
Daiqiang Li ◽  
Qiong Zou ◽  
Yuan Yuan ◽  
...  
Keyword(s):  
Overall Survival ◽  
Prognostic Factors ◽  
Survival Time ◽  
Biliary Tract ◽  
Extrahepatic Cholangiocarcinoma ◽  
Positive Correlation ◽  
Overall Survival Time ◽  
Positive Expression ◽  
Dismal Prognosis ◽  
Clinicopathological Significance

Background: To evaluate the expression and clinicopathological significance of a disintegrin and metalloproteinases 19 (ADAM19) CUE domain containing protein 2 (CUEDC2) in extrahepatic cholangiocarcinoma (EHCC). Materials & methods: Immunostaining of ADAM19 and CUEDC2 was performed by EnVision immunohistochemistry in benign and malignant biliary tract tissues. Result: The expression of ADAM19 and CUEDC2 were significantly higher in EHCC (p < 0.05). ADAM19 expression was positive correlated with CUEDC2 expression in EHCC (p < 0.05). The overall survival time of those with positive expression of ADAM19 and CUEDC2 was lower (p < 0.001). Both positive expression of ADAM19 and CUEDC2 were independent prognostic factors in EHCC. Conclusion: ADAM19 and CUEDC2 have a positive correlation to the pathogenesis and dismal prognosis in EHCC.

Long-Term Prognostic Value of Hematological and “Old” Biological Parameters in CLL. Reflexions on 156 Patients with a Median Follow-Up Time More Than 6.5 Years.

Blood ◽  
2006 ◽  
Vol 108 (11) ◽  
pp. 4951-4951
Author(s):  
Réda Garidi ◽  
Ioana Vaida ◽  
Jean-Claude Capiod ◽  
Salhia Sid Idris ◽  
Bernard Desablens
Keyword(s):  
Prognostic Factors ◽  
Survival Time ◽  
Median Survival Time ◽  
Prognostic Value ◽  
Cox Model ◽  
Hematological Parameters ◽  
Biological Parameters ◽  
Prognostic System ◽  
System P

Abstract Waiting for a standardization of “modern” biological prognostic factors in CLL, such as mutational status, ZAP-protein, CD38… we review with a long median delay of survey, the prognostic value of classical hematological data and “old” biological parameters such as seric LDH, β2-microglobuline, sCD23 and sCD25. At time of diagnosis our 156 pts were aged from 39 to 80 yrs (median=66 yrs) and sex-ratio shows as usually a male predominance (M/F=1.40). According to Binet’s system we note 131 stages A (95 stages A0), 14 stages B and 11 stages C. With a median follow-up time more than 6.5 yrs, median survival time is 9.5 yrs and median “corrected” (i.e., without not CLL-related deaths including those due to a solid tumor because this complication is independent of initial prognostic factors of CLL) survival time is 10.2 yrs. When looking at “corrected” survival, best cut-off values for “old” biological factors are 1.25 N for LDH, 3 mg for β2-m, 33 U (=10 N) for sCD23 and 2282.5 U (=1.25 N) for sCD25. Cox model with these 4 pejorative factors (PF) retains sCD23 (p=0.00004), β2-m (p=0.00002) and LDH (p=0.001). When adding these 3 PF, we can build a very strong prognostic system: median survival time of the 117 pts with 0 or 1 PF is more than 11 yrs versus 4 yrs for the 39 pts with 2 PF or more (p<10−6). Among hematological parameters, Cox model retains 3 of them: platelets level at 2 cut-off values 150 and 100 G (p=0.000005 and 0.02); 3 or more lymphoid area according to Binet’s classification (p=0.0002) and hemoglobin level at cut-off value 120 g (p=0.03). DLT is also a strong prognostic factor (p= 0.002) but this value was missing for 47 pts. However Cox model done with DLT retains 3 factors: DLT (p=0.0003); hemoglobin at cut-off value 100 g (p=0.0003) and platelets at cut-off value 150 G (p=0.001). Cox model including “old” biological PF and classical hematological parameters without DLT, retains sCD23 (p=0.0003), platelet at cut-off value 150 G (p=0.0005) and 3 or more lymphoid areas (p = 0.003). Finally we test 3 clinical classifications (Rai, Binet and our local classification based on clinical examination, hemoglobin and platelet levels, and medullary examination) and 2 biological classifications (LDH+β2-m system and this new PF system). Cox model retains the sCD23+LDH+β2-m system (p=0.00009) and our local system (p=0.003) and when looking at global survival our prognostic system seems more performing than PF system: p=0.0007 and 0.001. Addition of LDT does not modify our results but this parameter is kept in Cox model too. We conclude on the necessity to clearly determine the best cut-off values for all prognostic parameters, classical and “old” and “new” biological ones. We also claim the usefulness to compare all prognostic factors of CLL together because “old” clinical system and DLT are perhaps more useful for clinical practice when patients are out of a prospective therapeutic trial.

scholarly journals Histopathological Evaluation and Analysis of Immunohistochemical Expression of Bcl-2 Oncoprotein in Colorectal Carcinoma

2019 ◽  
Vol 14 (4) ◽  
pp. 317-321
Author(s):  
Shilpa Tukaram Patil ◽  
Clement Wilfred Devadass ◽  
Prasanna Shetty Badila
Keyword(s):  
Prognostic Factors ◽  
Colorectal Carcinoma ◽  
Tumor Grade ◽  
Colorectal Carcinomas ◽  
Statistical Association ◽  
Immunohistochemical Expression ◽  
Female Ratio ◽  
Male Female Ratio ◽  
Apoptotic Marker ◽  
Advanced Stages

Introduction: Colorectal cancer is the third most prevalent malignancy with high mortality rate, necessitating markers that predict survival and guide the treatment. Previous studies have examined the immunohistochemical expression of Bcl-2, an apoptotic marker, in colorectal carcinoma, but results have been contradictory. Objectives: To evaluate the histopathological features of colorectal carcinoma, analyze the immunohistochemical expression of Bcl-2, and to find out statistical association of Bcl-2 expression with certain prognostic factors histopathologic type, grade and stage. Material and Methods: This prospective study was conducted on the colectomy specimens of colorectal carcinoma, over a period of two years. The tumor morphology and Bcl-2 status were evaluated by immunohistochemistry in each case. Results: The study included 58 cases, with mean patient age of 47.07 years and male: female ratio of 1.89:1. Bcl-2 positivity was seen in 32.7% of cases. Weak, moderate, and strong expression of Bcl-2 was seen in 12.1%, 12.1%, and 8.5% of cases respectively. Even though early stages of colorectal carcinoma showed greater frequency of Bcl-2 expression than advanced stages (36.3% versus 28%), this association was not statistically significant. Conclusion: Lack of statistically significant correlation between Bcl-2 immunohistochemical expression and prognostic parameters like tumor grade and stage, suggests that Bcl-2 immunoexpression may not be a significant prognostic marker in colorectal carcinoma. Key words: Colorectal carcinomas; Immunohistochemistry; Prognostic factors.

Re-Evaluation of Prognostic Value of Seric LDH and β2-Microglobulin Levels among 248 CLL with a Median Follow-Up Time More Than 6 Years.

Blood ◽  
2006 ◽  
Vol 108 (11) ◽  
pp. 4441-4441
Author(s):  
Bernard Desablens ◽  
Réda Garidi ◽  
Bérengère Gruson ◽  
Iona Vaida ◽  
Marie Brevet
Keyword(s):  
Prognostic Factors ◽  
Survival Time ◽  
Median Survival ◽  
Prognostic Value ◽  
Cox Model ◽  
P Value ◽  
Survival Times ◽  
Prognostic System ◽  
Md Anderson

Abstract Except in MD Anderson Center, seric β2-m is not recognized as a strong prognostic factor in CLL and seric LDH too, despite this factor is more and more important among lymphoma with a continuous prognostic value. So we studied 248 CLL aged less than 75 yrs, without elevated creatininemia or evident hemolysis. There were 201 pts with stage A, 29 B and 18 C and sex-ratio M/F was 1.48. Median survival time is 10 yrs and median “corrected” (i.e., without not CLL-related deaths including those due to a solid tumor because this complication is independent of initial prognostic factors of CLL) survival time is 10.9 yrs. LDH values range from 0.55 to 3.54 N, and mean and median values are 1.05±0.40 and 0.96 N. LDH level is only correlated with blood lymphocytosis and hemoglobin levels studied as continuous values (p=0.015 and 0.002). When looking at “corrected” survival, all cut-off values from 0.75 N, N, 1.25 N… to 2.5 N are statistically significant but the best one is 1.25 N (p=6 10−6). β2-m values range from 0.81 to 16.5 mg per l, and mean and median values are 2.67±1.75 and 2.15 mg. When studied as continuous values, β2-m is linked to age, number of lymphoid areas according to Binet’s system, and hemoglobin and platelets levels (p=0.0009, <10−4, <10−4 and 0.003) but age was ruled out among patients less than 70 yrs. For “corrected” survival, all cut-off values except 1.25 mg were significant and for example p value at 4 mg level as usually used by MD Anderson Center, is 5 10−5. However we find that the best cut-off value is 3 mg (p<10−6) and this value is statistically independent from LDH one in Cox model. We tried to combine these 2 cut-off values. Product of observed LDH/1.25 N × observed β2-m/3 mg were pertinent to isolate pts with a very poor prognosis (median “corrected” survival time about 6 yrs) but size of pejorative subgroups were small (≈25% of all pts) and other subgroups were not statistically significant. When adding ratios, results were similar. So we find that the best system is to count the number of pejoratives factors, i.e., LDH > 1.25 N and β2-m > 3 mg. Global median survival times of the 3 defined groups were respectively > 10.8 yrs (160 pts), 6.5 yrs (68 pts) and 4.2 yrs (20 pts) with a p value < 10−6. This system is also efficient among stage A pts (p=10−5). When we compare this prognostic system with Binet’s and Rai’s prognostic systems, Cox model rules out Binet’s one and keeps Rai’s system and ours (p=0.01 and 0.0005). Among patients with stage A, Cox model keeps only this LDH and β2-m system. We conclude on the strong prognostic values of seric LDH and β2-m initial levels in CLL and we claim that these 2 simple biological parameters have to been compared with “modern” biological prognostic factors of CLL.

scholarly journals Radiomics and Dosiomics for Predicting Local Control after Carbon-Ion Radiotherapy in Skull-Base Chordoma

Cancers ◽  
2021 ◽  
Vol 13 (2) ◽  
pp. 339
Author(s):  
Giulia Buizza ◽  
Chiara Paganelli ◽  
Emma D’Ippolito ◽  
Giulia Fontana ◽  
Silvia Molinelli ◽  
...  
Keyword(s):  
Prognostic Factors ◽  
Skull Base ◽  
Local Control ◽  
Tumour Progression ◽  
Risk Groups ◽  
Carbon Ion Radiotherapy ◽  
Survival Models ◽  
Carbon Ion ◽  
Combined Models ◽  
Skull Base Chordoma

Skull-base chordoma (SBC) can be treated with carbon ion radiotherapy (CIRT) to improve local control (LC). The study aimed to explore the role of multi-parametric radiomic, dosiomic and clinical features as prognostic factors for LC in SBC patients undergoing CIRT. Before CIRT, 57 patients underwent MR and CT imaging, from which tumour contours and dose maps were obtained. MRI and CT-based radiomic, and dosiomic features were selected and fed to two survival models, singularly or by combining them with clinical factors. Adverse LC was given by in-field recurrence or tumour progression. The dataset was split in development and test sets and the models’ performance evaluated using the concordance index (C-index). Patients were then assigned a low- or high-risk score. Survival curves were estimated, and risk groups compared through log-rank tests (after Bonferroni correction α = 0.0083). The best performing models were built on features describing tumour shape and dosiomic heterogeneity (median/interquartile range validation C-index: 0.80/024 and 0.79/0.26), followed by combined (0.73/0.30 and 0.75/0.27) and CT-based models (0.77/0.24 and 0.64/0.28). Dosiomic and combined models could consistently stratify patients in two significantly different groups. Dosiomic and multi-parametric radiomic features showed to be promising prognostic factors for LC in SBC treated with CIRT.

Prognostic Factors of Pulmonary Metastasectomy for Colorectal Carcinomas

2009 ◽  
Vol 33 (3) ◽  
pp. 505-511 ◽  
Author(s):  
Fengshi Chen ◽  
Nobuharu Hanaoka ◽  
Kiyoshi Sato ◽  
Takuji Fujinaga ◽  
Makoto Sonobe ◽  
...  
Keyword(s):  
Prognostic Factors ◽  
Pulmonary Metastasectomy ◽  
Colorectal Carcinomas

Relationship between MUC5AC and altered expression of MLH1 protein in mucinous and non-mucinous colorectal carcinomas

2004 ◽  
Vol 200 (5) ◽  
pp. 371-377 ◽  
Author(s):  
Lorena Losi ◽  
Alessandra Scarselli ◽  
Piero Benatti ◽  
Maurizio Ponz de Leon ◽  
Luca Roncucci ◽  
...  
Keyword(s):  
Colorectal Carcinomas ◽  
Altered Expression ◽  
Mlh1 Protein

scholarly journals A Retrospective Study of the Clinical Phenotype and Predictors of Survival in non-Caucasian Hispanic Patients with Amyotrophic Lateral Sclerosis.

2019 ◽  
Author(s):  
Claudia Marisol Sánchez-Martínez ◽  
José Alberto Choreño-Parra ◽  
Lilia Nuñez-Orozco ◽  
Noel Isaías Placencia-Álvarez ◽  
Laura Marcela Alvis-Cataño ◽  
...  
Keyword(s):  
Overall Survival ◽  
Amyotrophic Lateral Sclerosis ◽  
Prognostic Factors ◽  
Retrospective Study ◽  
Survival Time ◽  
Clinical Characteristics ◽  
Clinical Phenotype ◽  
Age At Onset ◽  
Als Patients ◽  
Lateral Sclerosis

Abstract Background. Little is known about the clinical phenotype of amyotrophic lateral sclerosis (ALS) in non-Caucasian populations. Here, we aimed to describe the clinical characteristics, prognostic factors and survival of Mexican patients with ALS. Methods. We conducted a retrospective study by reviewing the medical records of patients with ALS that attended and were regularly followed at a third level hospital in Mexico City from 2000 to 2015. We calculated absolute and relative frequencies of the clinical characteristics from all the participants. We also estimated correlation coefficients between clinical features and overall survival. Additionally, survival rates were compared for all participants grouped according to different clinical features using the Kaplan-Meier method and the log-rank test. Results. We enrolled 45 ALS patients, 53.33% had spinal-onset ALS and 46.66% presented bulbar ALS. The male/female ratio was 0.8. The mean age at onset of symptoms was 58.11 years. Mean survival time from onset was 64.73 ± 34.83 months. Cumulative survival rate after 5 years of disease onset was 44.44%. Age at onset and age at diagnosis inversely correlated with overall survival time. Also, we found that bulbar-onset, short diagnostic delay, percutaneous endoscopic gastrostomy, mechanical ventilation, and lower total cholesterol serum levels were associated with short survival. Conclusions. The clinical characteristics of Mexican ALS patients differ from the disease phenotype observed in Caucasians. Nonetheless, the predictive value of certain well-recognized prognostic factors remains consistent in our population. The current study provides relevant information for a better understanding of prognostic factors in ALS patients from Mexico and other Latin American countries.

scholarly journals Bioinformatic Analysis: The Role of LINC00634 in Colorectal Carcinoma

2021 ◽  
Author(s):  
Fang Liu ◽  
Fengyihuan Fu ◽  
Yuqiang Nie
Keyword(s):  
Esophageal Cancer ◽  
Regression Analysis ◽  
Prognostic Factors ◽  
Colorectal Carcinoma ◽  
Roc Curve ◽  
Cox Regression ◽  
Survival Outcomes ◽  
Immune Infiltration ◽  
The Relationship

Abstract Background: LINC00634 is highly expressed in esophageal cancer, and its depletion can suppress the viability and induce the apoptosis of esophageal cancer cells. However, there is a lack of studies that examine the relationship between LINC00634 expression and the clinicopathological features, survival outcomes, prognostic factors and tumor immune cell infiltration of colorectal carcinoma (CRC) patients.Objective: We aim at investigating the role of LINC00634 in colorectal carcinoma.Methods: We obtained data from the TCGA (The Cancer Genome Atlas) public database, GTEx (Genotype-Tissue Expression) database and clinical samples. Wilcoxon rank-sum test, Kruskal-Wallis test and logistic regression analysis were employed to assess the relationship between LINC00634 expression and the clinicopathological characteristics of CRC patients. Receiver operating characteristic (ROC) curve was constructed to evaluate the ability of LINC00634 for distinguishing between CRC patients and normal subjects based on the area under the curve (AUC) score. Univariate and multivariate analyses were conducted to evaluate the association between prognostic factors and survival outcomes. Kaplan-Meier curves and Cox regression analysis were employed to determine the contribution of LINC00634 expression to the prognosis of colorectal carcinoma patients. Immune infiltration analysis and Gene Set Enrichment Analysis (GSEA) were conducted to identify the significantly involved functions of LINC00634. Finally, a nomogram was constructed for internal verification based on the Cox regression data.Results: The expression of LINC00634 was upregulated in CRC patients, and markedly associated with N stage, residual tumor, pathological stage, and overall survival (OS) event. ROC curve showed that LINC00634 had strong diagnostic and prognostic abilities (AUC=0.74). The high expression of LINC00634 could predict poor disease specific survival (DSS; P=0.008) and poor overroll survival (OS;P<0.01). The expression of LINC00634 was independently associated with OS in CRC patients (P=0.019). GSEA and immune infiltration analysis demonstrated that LINC00634 expression was involved in gene transcription, epigenetic regulation and the functions of certain types of immune infiltrating cells. The c-index of the nomogram was 0.772 (95％CI: 0.744-0.799).Conclusions: Our study reveals that LINC00634 can serve as a potential prognostic biomarker for CRC patients.

The Clinical and Prognostic Significance of Protein Arginine Deiminases 2 and 4 in Colorectal Cancer

Pathobiology ◽  
2021 ◽  
pp. 1-11
Author(s):  
Mohamed Gijon ◽  
Rachael L. Metheringham ◽  
Michael S. Toss ◽  
Samantha J. Paston ◽  
Lindy G. Durrant
Keyword(s):  
Colorectal Cancer ◽  
Overall Survival ◽  
Multivariate Analysis ◽  
Prognostic Factors ◽  
Survival Time ◽  
Patient Survival ◽  
Prognostic Significance ◽  
High Expression ◽  
Related Protein ◽  
Post Translational Modification

<b><i>Introduction:</i></b> Protein arginine deiminases (PADIs) are a family of enzymes that catalyse the post-translational modification of proteins. Association between PADI expression and clinicopathology, protein expression, and outcome was determined. <b><i>Methods:</i></b> PADI2 and PADI4 expression was assessed immunohistochemically in a cohort of colorectal cancer (CRC) patients. <b><i>Results:</i></b> CRC tissues expressed variable levels of PADI2 which was mainly localised in the cytoplasm and correlated with patient survival (<i>p</i> = 0.005); high expression increased survival time from 43.5 to 67.6 months. Expression of cytoplasmic PADI2 correlated with the expression of nuclear β catenin, PADI4, and alpha-enolase. In contrast, expression of nuclear PADI2 correlated with a decrease in survival (<i>p</i> = 0.010), with high expression decreasing survival from 76.4 to 42.9 months. CRC tissues expressed variable levels of PADI4 in both the nucleus and cytoplasm. Expression of cytoplasmic PADI4 correlated with survival (<i>p</i> = 0.001) with high expression increasing survival time from 48.1 to 71.8 months. Expression of cytoplasmic PADI4 correlated with expression of nuclear β catenin, alpha-enolase (<i>p</i> ≤ 0.0001, <i>p</i> = 0.002), and the apoptotic related protein, Bcl-2. Expression of nuclear PADI4 also correlated with survival (<i>p</i> = 0.011), with high expression of nuclear PADI4 increasing survival time from 55.4 to 74 months. Expression of nuclear PADI4 correlated with p53, alpha-enolase, and Bcl-2. Multivariate analysis showed that TNM stage, cytoplasmic PADI2, and PADI4 remained independent prognostic factors in CRC. Both PADI2 and PADI4 are good prognostic factors in CRC. <b><i>Conclusion:</i></b> High expression of cytoplasmic PADI2, PADI4, and nuclear PADI4 were associated with an increase in overall survival.

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