Glycosylation Changes on Serum Glycoproteins in Ovarian Cancer May Contribute to Disease Pathogenesis

Ovarian cancer is the most lethal of all gynaecological cancers among women. Serum CA125 is the only biomarker that is used routinely and there is a need for further complementary biomarkers both in terms of sensitivity and specificity.N-glycosylation changes in ovarian cancer serum glycoproteins include a decrease in galactosylation of IgG and an increase in sialyl Lewis X (SLex) on haptoglobinβ-chain,α1-acid glycoprotein andα1-antichymotrypsin. These changes are also present in chronic inflammation but not in malignant melanoma, where there are low levels of inflammatory processes. Acute phase proteins carrying increased amounts of SLexhave an increased half-life. Sialylation of acute phase proteins also decreases apoptosis favouring survival of cancer cells. Cancer cells produce inflammatory cytokines which influence glycosylation processing in liver parenchymal cells. Altered glycosylation of the acute phase protein transferrin plays an important role in iron homeostasis. Glycosylated transferrin and its glycans have anti-apoptotic properties and many transferrin receptors in carcinoma could play a role in development of anaemia. Decreased galactosylation and sialylation of IgG increases the cytotoxicity of natural killer cells and complement activation via mannose-binding lectin (MBL). Altered glycosylation of acute phase proteins and IgG suggests that cancer regulates certain pathways favouring cancer cells survival.

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A Robust and Versatile Automated Glycoanalytical Technology for Serum Antibodies and Acute Phase Proteins: Ovarian Cancer Case Study

Molecular & Cellular Proteomics ◽

10.1074/mcp.ra119.001531 ◽

2019 ◽

Vol 18 (11) ◽

pp. 2191-2206 ◽

Cited By ~ 2

Author(s):

Róisín O'Flaherty ◽

Mohankumar Muniyappa ◽

Ian Walsh ◽

Henning Stöckmann ◽

Mark Hilliard ◽

...

Keyword(s):

Ovarian Cancer ◽

Acute Phase ◽

Acute Phase Proteins ◽

Cancer Case ◽

Serum Antibodies ◽

Ovarian Cancer Case

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Inhibition of the activity of pro-inflammatory secretory phospholipase A2by acute phase proteins

Mediators of Inflammation ◽

10.1155/s0962935196000270 ◽

1996 ◽

Vol 5 (3) ◽

pp. 196-201 ◽

Cited By ~ 1

Author(s):

W. Pruzanski ◽

E. Stefanski ◽

P. Vadas

Keyword(s):

Acute Phase ◽

Inhibitory Activity ◽

Acute Phase Proteins ◽

Acute Phase Reaction ◽

Phase Reaction ◽

Protective Mechanisms ◽

Spla2 Activity ◽

Pancreatic Phospholipase ◽

Inflammatory Processes ◽

Bacteriostatic Property

Pro-Inflammatory non-pancreatic phospholipase A2(sPLA2) is markedly over-expressed in acute systemic and chronic local inflammatory processes. Since in acute phase reaction sPLA2is often over-expressed simultaneously with acute phase proteins (APP), it is important to determine whether APP interacts with sPLA2. We tested ten APPs for interaction with sPLA2using as a substrate multilamellar Hposomes composed either of PC:Lyso PC or PE:Lyso PE. Using PC:Lyso PC substrate, CRP, lactoferrin and SAP were found to inhibit sPLA2activity with an IC50of 25 μg/ml, 7.5 μg/ml and 50 μg/ml, respectively, corresponding to 0.21 μM, 0.1 μM and 0.21 μM respectively. Using PE:Lyso PE substrate only SAP was inhibitory, with an IC50of 10 μg/ml (0.04 μM). Phosphorylcholine abolished the inhibitory activity of CRP but not of SAP or lactoferrin. Addition of phosphorylethanolamine or of excess calcium had no effect on the inhibitory activity of APP. Limulin, lysozyme, transferrin, β2-microglobulin, α2-macroglobulin, human and bovine albumins had no effect on sPLA2 activity. Therefore neither the structure of pentraxins, or ironbinding, bacteriostatic property or amyloidogenic property preclude whether APP modulates sPLA2activity. Inhibition of pro-inflammatory sPLA2by APP may be one of the protective mechanisms of the acute phase reaction.

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FUNCTIONAL ROLE OF ACUTE PHASE PROTEINS IN CANCER CELLS

Biochemical Society Transactions ◽

10.1042/bst024537sb ◽

1996 ◽

Vol 24 (4) ◽

pp. 537S-537S

Author(s):

Soo-Young Lee ◽

Joo-Weon Lim ◽

Jeong-Hee Kang

Keyword(s):

Cancer Cells ◽

Acute Phase ◽

Functional Role ◽

Acute Phase Proteins

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Diagnosis value of some acute-phase proteins in purulent-inflammatory diseases of uterine appendages

Bulletin of Siberian Medicine ◽

10.20538/1682-0363-2006-3-112-116 ◽

2006 ◽

Vol 5 (3) ◽

pp. 112-116

Author(s):

S. V. Shramko ◽

S. V. Arkhipova ◽

L. G. Bazhenova ◽

R. M. Zorina

Keyword(s):

Acute Phase ◽

Acute Phase Proteins ◽

Inflammatory Diseases ◽

Treatment Options ◽

Organic Destruction ◽

Monospecific Antiserum ◽

Inflammatory Processes ◽

Diagnostic Criterion ◽

The Given ◽

Selection Of

We investigated concentrations of acute-phase proteins a-2-macroglobulin (MG) and lactoferrin (LF) in blood serum of 78 women with various types of uterine appendages inflammatory processes. Coefficient MG/LF was used as an additional diagnostic criterum of purulent-necrotic destruction of organs and tissues and allowed us to choose proper treatment options. MG values were assessed by method of rocket immune electroforesis using monospecific antiserum to the given protein, LF level was assessed by enzyme linked immunoassay based method (ELISA). Standard was performed when coefficient MG/LF was greater than 1, and if value of coefficient MG/LF was less than 1, we performed surgical treatment. Using coefficient MG/LF as a diagnostic criterion of existence of organic destruction in uterine appendages allowed us to optimize the selection of treatment program.

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Changes in serum acute phase proteins in ovarian cancer patients receiving cis-diamminedichloroplatinum (CDDP) infusion therapy

Clinical Biochemistry ◽

10.1016/s0009-9120(84)90465-x ◽

1984 ◽

Vol 17 (1) ◽

pp. 39-41 ◽

Cited By ~ 7

Author(s):

R.G. Fish ◽

T.S. Gill ◽

M. Adams ◽

F. Faqeera ◽

I. Kerby

Keyword(s):

Ovarian Cancer ◽

Cancer Patients ◽

Acute Phase ◽

Acute Phase Proteins ◽

Infusion Therapy

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Cytokine elevations in acute coronary syndrome and ovarian cancer: A mechanism for the up-regulation of the acute phase proteins in these different disease etiologies

Clinical Biochemistry ◽

10.1016/j.clinbiochem.2008.01.019 ◽

2008 ◽

Vol 41 (7-8) ◽

pp. 607-610 ◽

Cited By ~ 13

Author(s):

Peter A. Kavsak ◽

Agnes Lee ◽

Hal Hirte ◽

Ed Young ◽

Jack Gauldie

Keyword(s):

Ovarian Cancer ◽

Acute Coronary Syndrome ◽

Acute Phase ◽

Acute Phase Proteins ◽

Coronary Syndrome

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THE EFFECTS OF STRESS ON ACUTE PHASE PROTEINS OF CATTLE

Scientific Messenger of LNU of Veterinary Medicine and Biotechnology ◽

10.15421/nvlvet7202 ◽

2016 ◽

Vol 18 (4(72)) ◽

pp. 107-113

Author(s):

S.M. Sevgisunar ◽

S. Şahinduran

Keyword(s):

Acute Phase ◽

Serum Amyloid A ◽

Acute Phase Proteins ◽

Clinical Signs ◽

Stress Factors ◽

Stress Effect ◽

Hormonal Changes ◽

Acid Glycoprotein ◽

Amyloid A ◽

Inflammatory Processes

Stress occurs with the pressure of external and internal (infectious or non–infectious) causes which forces change in animals. This stress not only emerge with behavioral or clinical signs, but also will result with immune response formed by body. Understanding and evaluating this subclinic response is also possible with the help of Acute Phase Proteins (APP–AFP) which are released by tissues and organs when exposed to external and internal changes as well as known hormonal changes. Cattle are fed because of their economical importance and the inflammatory processes have influence on their yield levels. Because the stress is laying under all these health problems, also it is getting attention how stress effect the animals. Stress can be divided into main four groups: physical (mechanical), inflammatory (infectious), behavioral and other stress factors. The main APPs which all groups mostly using in their studies are serum amyloid A (SAA) haptoglobin (Hp), ceruloplasmin (Cp), fibrinogen (Fb), lipopolisakkarit binding protein (LBP) ve α1–Acid Glycoprotein (AGP).

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