scholarly journals Recent Applications of Ion Mobility Spectrometry in Diagnosis of Vaginal Infections

2012 ◽  
Vol 2012 ◽  
pp. 1-6 ◽  
Author(s):  
Zeev Karpas ◽  
Gabi Cohen ◽  
Elias Atweh ◽  
Geoff Barnard ◽  
Moshe Golan
Keyword(s):  
Ion Mobility Spectrometry ◽  
Ion Mobility ◽  
Vaginal Discharge ◽  
Reproductive Age ◽  
Sample Introduction ◽  
Vaginal Infections ◽  
Female Population ◽  
High Proton Affinity ◽  
High Proton ◽  
Recent Developments

Vaginal infections (vaginosis) globally affect more than 15% of the female population of reproductive age. However, diagnosis of vaginosis and differentiating between the three common types: bacterial vaginosis (BV), vulvovaginal candidiasis (VVC), and trichomoniasis are challenging. Elevated levels of the biogenic amines, trimethylamine (TMA), putrescine, and cadaverine have been found in vaginal discharge fluid of women with vaginosis. Ion mobility spectrometry (IMS) is particularly suitable for measurement of amines even in complex biological matrices due to their high proton affinity and has been shown to be suitable for the diagnosis of vaginal infections. Recent developments that have increased the accuracy of the technique for diagnosis of BV and simplified sample introduction are described here.

scholarly journals Bacterial vaginosis, the leading cause of genital discharge among women presenting with vaginal infection in Dar es Salaam, Tanzania

2021 ◽  
Vol 21 (2) ◽  
pp. 531-537
Author(s):  
Mtebe V Majigo ◽  
Paschal Kashindye ◽  
Zachariah Mtulo ◽  
Agricola Joachim
Keyword(s):  
Bacterial Vaginosis ◽  
Low Income ◽  
Vaginal Discharge ◽  
Dar Es Salaam ◽  
Referral Hospital ◽  
Reproductive Age ◽  
Low Income Countries ◽  
Vaginal Infection ◽  
Vaginal Infections ◽  
Regional Referral Hospital

Background: Pathological vaginal discharge is a common complaint of women in reproductive age worldwide caused by various agents. The prevalence and etiologic agents vary depending on the population studied. Management of vaginal discharge in low-income countries, typically depend on the syndromic approach, which limits understanding the specific causative agents. We determined the proportion of bacterial vaginosis, candidiasis, and trichomoniasis among women with vaginal discharge at a regional referral hospital in Dar es Salaam, Tanzania. Methods: We conducted a cross-sectional study between June and August of 2017 among nonpregnant women at Amana Regional Referral Hospital. Experienced staff performed physical examination to establish a clinical diagnosis, and collection of the high vaginal swab for microscopic examination. Descriptive statistics were performed to assess the characteristics of study participants and the proportion of vaginal infections. Results: A total of 196 samples were collected, of all, 128 (65.3%) had either bacterial vaginosis, candidiasis, or trichomo- niasis. Bacterial vaginosis was the leading infection at 33.2%, followed by candidiasis (19.4%) and trichomoniasis (13.3%). Laboratory confirmed vaginal infection were generally found more in age below 25, unmarried, and those employed or petty business. Conclusion: The proportion of bacterial vaginosis in women with vaginal discharge was relatively higher than others, and the presence of vaginal infection relate to socio-demographic characteristics. Further advanced studies are needed to understand the potential role of aetiologic agents in causing vaginal infections. Keywords: Bacterial vaginosis; vaginal discharge; genital infection.

Novel application for ion mobility spectrometry: diagnosing vaginal infections through measurement of biogenic amines

2002 ◽  
Vol 474 (1-2) ◽  
pp. 115-123 ◽  
Author(s):  
Zeev Karpas ◽  
Walter Chaim ◽  
Rachel Gdalevsky ◽  
Boris Tilman ◽  
Avi Lorber
Keyword(s):  
Biogenic Amines ◽  
Ion Mobility Spectrometry ◽  
Ion Mobility ◽  
Vaginal Infections

scholarly journals BACTERIAL VAGINOSIS;

2017 ◽  
Vol 24 (02) ◽  
pp. 252-257
Author(s):  
Faiqa Saleem ◽  
Munazza Malik ◽  
Muhammad Sohaib Shahid ◽  
Muhammad Tayyab
Keyword(s):  
Bacterial Vaginosis ◽  
Vaginal Discharge ◽  
Reproductive Age ◽  
P Value ◽  
Vaginal Infections ◽  
Vaginal Gel ◽  
Vaginal Cream ◽  
Significant Difference ◽  
Group A ◽  
Group B

In women of reproductive age bacterial vaginosis is a most common polymicrobialdisease and is the leading reason for vaginal discharge in this age group. Additionally itis further linked with sizeable disease burden of community problem in terms of infectiouscomplications. Clindamycin vaginal cream and metronidazole vaginal gel are effective in themanagement of vaginal infections caused by multi bacteria. Objectives: To compare thetherapeutic efficacy of Metronidazole vaginal gel and clindamycin vaginal cream as modality oftreatment for bacterial vaginosis. Study Design: Randomized control trial. Setting: Departmentof Obstetrics and Gynaecology, Unit-3 Jinnah Hospital Lahore. Period: Six months from 02-05-2011 to 01-11-2011. Material & Methods: A total of 300 patients were included in this study.They were divided into two groups. Group A received metronidazole vaginal gel (5 g dailyfor 7 days) while group B administered with clindamycin vaginal cream (5g daily for 7 days).Results: Mean age of the patient was observed 34.3+3.5 and 32.9+ 2.1 years in group –A andB respectively. Vaginal discharge was absent in 104 patients (69.3%) from group A and 127(84.7%) from group-B. Absence of clue cells on microscopy revealed in 112 patients (74.7%)from group A and 137 patients (91.3%) from group B. Absence of amine odour found in 116patients (77.3%) of group A and 134 patients (89.3%) of group B. Significant difference wasfound between two groups with p value of 0.006 in respect of efficacy. Conclusion: Clindamycinvaginal cream is more effective in comparison to Metronidazole vaginal gel for the treatment ofbacterial vaginosis.

Recent Developments in Ion Mobility Spectrometry

2011 ◽  
Vol 46 (6) ◽  
pp. 472-521 ◽  
Author(s):  
Helko Borsdorf ◽  
Thomas Mayer ◽  
Mashaalah Zarejousheghani ◽  
Gary A. Eiceman
Keyword(s):  
Ion Mobility Spectrometry ◽  
Ion Mobility ◽  
Recent Developments

scholarly journals Colposcopic and microbiological correlation in patients with vaginal infections: a prospective study

2021 ◽  
Vol 9 (4) ◽  
pp. 1174
Author(s):  
Reena Sharma ◽  
Manjit Kaur Mohi ◽  
Khush Preet Kaur ◽  
Geeta Walia
Keyword(s):  
Vaginal Discharge ◽  
Significant Proportion ◽  
Vaginal Flora ◽  
Reproductive Age ◽  
Microbiological Analysis ◽  
Age Group ◽  
Vaginal Infections ◽  
Symptoms And Signs ◽  
Abnormal Vaginal Flora ◽  
Reproductive Age Group

Background: Objective of the study was to evaluate the association of abnormal vaginal flora with pathological colposcopic and microbiological findings.Methods: The present study was conducted in outpatient’s department of Obstetrics and Gynaecology, Government Medical College, Patiala. We studied 300 cases with symptoms and signs of vaginal infections in reproductive age group (15-49 years). After taking detailed history and examination, samples of vaginal discharge were collected for microbiological analysis and patients were subjected to colposcopy. Recorded data was analysed to find out any association of abnormal vaginal flora with pathological colposcopic and microbiological analysis.Results: The prevalence rate of vaginal infections was 31% among patients who reported to us with symptoms and signs of vaginal infections. The most common micro-organism detected was B. vaginosis (BV) in 14%, C. albicans (C) in 12% and T. vaginalis (T) in 3.33%. The normal colposcopic findings were observed in 81.33% and abnormal colposcopic findings were noted in 18.67% of the patients which were vaginal hyperaemia in 9%, chronic cervicitis in 5.33%, small iodine negative area in 2.33%, keratosis in 1.33% and cervical polyp in 0.67% of the patients.Conclusions: We concluded that vaginal discharge is a common gynaecological complaint and vaginal infections are an important cause of the vaginal discharge thus leading to significant proportion of female morbidity in reproductive age group. So, vaginitis and vaginal discharge highlights the importance of microbiological investigations to find out the causative organisms and the specific findings due to particular micro-organism can be correlated colposcopically and specific treatment can be provided.

scholarly journals MICROBIOLOGICAL PROFILE OF VAGINOSIS AMONG WOMEN OF THE REPRODUCTIVE AGE GROUP, WHO ATTENDED AT DMC, LAHERIASARAI, BIHAR

2020 ◽  
pp. 82-85
Author(s):  
Neha Jha ◽  
R. S. Prasad ◽  
P. N. Jha ◽  
Debarshi Jana
Keyword(s):  
Bacterial Vaginosis ◽  
Vaginal Discharge ◽  
Cross Sectional Study ◽  
Reproductive Age ◽  
Yeast Cells ◽  
Age Group ◽  
Vaginal Infections ◽  
Cross Sectional ◽  
Abnormal Vaginal Discharge ◽  
Reproductive Age Group

Background: An abnormal vaginal discharge is a common complaint in women and it can be due to vaginal infections like bacterial vaginosis, candidiasis and trichomoniasis. Aim: A descriptive cross sectional study was done at Microbiology Department, DMC, Laheriasarai, Bihar to find out the common pathogens which caused vaginosis in patients of the reproductive age group, who attended the Obstetrics and Gynaecology Department at the Darbhanga Medical College and Hospital, Laheriasarai, Bihar and referred to Microbiology Department of DMC, from 1st April to 30th September, 2020. Materials and Methods: All the 140 vaginal discharge samples were subjected to gram staining to view the morphological nature of the bacteria which caused bacterial vaginosis and gram positive, budding yeast cells, wet film for the motility of Trichomonas and Chlamydial antigen detection by ELISA. Results: The overall positivity was 44%, which included bacterial vaginosis (36.4%). The positivity of candidiasis was 4.2%, that of trichomoniasis was 2.1% and that of chlamydiasis was 1.4%. Conclusion: Bacterial vaginosis was found to be the commonest cause of the abnormal vaginal discharge in women of the reproductive age group.

scholarly journals Gram stain and Giemsa stain as a rapid diagnostic tool for diagnosis of vaginal infections” in Western Nepal

2017 ◽  
Vol 5 (1) ◽  
pp. 5
Author(s):  
Kishor Bajracharya ◽  
Narinder Kaur ◽  
Sushama Suresh Pant
Keyword(s):  
Bacterial Vaginosis ◽  
Vaginal Discharge ◽  
Specific Treatment ◽  
Vaginal Swab ◽  
Giemsa Stain ◽  
Reproductive Age ◽  
Vaginal Infections ◽  
Medical College ◽  
Child Bearing ◽  
Litmus Paper

Abstract: Introduction: Infective vaginitis accounts for about 90% of all the cases of vaginal discharge in the women of reproductive age group. This includes triad of bacterial vaginosis, candidiasis and trichomoniasis. Method: The study was carried out in Lumbini Medical College and Teaching Hospital, Palpa, Nepal for a time period of 4 months. Total 125 high vaginal swabs from posterior fornix of vagina were collected from November 2014 to February 2015 in the department of Obstetrics and Gynecology, in the patients complaining of vaginal discharge. PH of the vaginal discharge was also determined by using litmus paper. Gram’s and Giemsa stain of vaginal swab smear were done to diagnose Bacterial vaginosis, Candidiasis and Trichomoniasis. Age, parity and pregnancy status of the patients were noted. Result: Out of 125 patient, bacterial vaginosis was found in 16% and candidiasis in 37%.Candidiasis and bacterial vaginosis were observed in child bearing sexually active women. Surprisingly bacterial vaginosis was not observed in pregnant women. Trichomoniasis infection was present in one case (0.8%) while severe invasive Candidiasis was observed in one woman. Conclusion: A definitive diagnosis can easily be made by Gram’s and Giemsa stain and microscopic examination of high vaginal swab. This may help in specific treatment and  prevent associated morbidity and  recurrence. Key words: Bacterial vaginosis, candidiasis, high vaginal swab, Gram’s stain, Giemsa stain.      

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