scholarly journals Positive Contrast Imaging of SPIO Nanoparticles

2012 ◽  
Vol 2012 ◽  
pp. 1-9 ◽  
Author(s):  
Chenghong Lin ◽  
Shuhui Cai ◽  
Jianghua Feng
Keyword(s):  
Negative Contrast ◽  
Superparamagnetic Iron Oxide ◽  
Positive Contrast ◽  
Disease Diagnosis ◽  
Cell Labeling ◽  
Contrast Imaging ◽  
Advantages And Disadvantages ◽  
Tissue Interface ◽  
Dark Signal ◽  
Ideal Method

It is advantageous to achieve positive contrast images instead of negative contrast images in superparamagnetic iron-oxide (SPIO) nanoparticles-based MR imaging in order to distinguish the signal surrounding SPIO nanoparticles from the dark signal due to local field inhomogeneity and the artifacts due to tissue interface and background noise, eliminate the inherent defects in the traditional MRI such as partial-volume effects and large void volume for reliable visualization, and increase contrast-to-noise ratio. Many methods generating positive signal with SPIO nanoparticles have been developed in the last decade. This paper provides an overview of current visualization methods and states their advantages and disadvantages. In practice, these techniques have been widely applied to cell labeling and disease diagnosis and monitoring. However, there is still a need for an ideal method to achieve both accuracy and sensitivity.

scholarly journals Comparison of Superparamagnetic and Ultrasmall Superparamagnetic Iron Oxide Cell Labeling for Tracking Green Fluorescent Protein Gene Marker with Negative and Positive Contrast Magnetic Resonance Imaging

2009 ◽  
Vol 8 (3) ◽  
pp. 7290.2009.00008 ◽  
Author(s):  
Zhuoli Zhang ◽  
Rohan Dharmakumar ◽  
Nicole Mascheri ◽  
Zhaoyang Fan ◽  
Shengyong Wu ◽  
...  
Keyword(s):  
Iron Oxide ◽  
Cell Function ◽  
Fluorescent Protein ◽  
Negative Contrast ◽  
Superparamagnetic Iron Oxide ◽  
Positive Contrast ◽  
Gfp Expression ◽  
Ultrasmall Superparamagnetic Iron Oxide ◽  
Contrast Magnetic Resonance ◽  
Green Fluorescent

The objectives of this study were to investigate the feasibility of imaging green fluorescent protein (GFP)-expressing cells labeled with iron oxide nanoparticles with the fast low-angle positive contrast steady-state free precession (FLAPS) method and to compare them with the traditional negative contrast technique. The GFP-R3230Ac cell line (GFP cell) was incubated for 24 hours using 20 μg Fe/mL concentration of superparamagnetic iron oxide (SPIO) and ultrasmall superparamagnetic iron oxide (USPIO) nanoparticles. Cell samples were prepared for iron content analysis and cell function evaluation. The labeled cells were imaged using positive contrast with FLAPS imaging, and FLAPS images were compared with negative contrast T2*-weighted images. The results demonstrated that SPIO and USPIO labeling of GFP cells had no effect on cell function or GFP expression. Labeled cells were successfully imaged with both positive and negative contrast magnetic resonance imaging (MRI). The labeled cells were observed as a narrow band of signal enhancement surrounding signal voids in FLAPS images and were visible as signal voids in T2*-weighted images. Positive contrast and negative contrast imaging were both valuable for visualizing labeled GFP cells. MRI of labeled cells with GFP expression holds potential promise for monitoring the temporal and spatial migration of gene markers and cells, thereby enhancing the understanding of cell- and gene-based therapeutic strategies.

Beyond DNA-targeting in Cancer Chemotherapy. Emerging Frontiers- A Review

2020 ◽  
Vol 20 ◽  
Author(s):  
S.N Mbugua ◽  
L.W Njenga ◽  
R.A Odhiambo ◽  
S.O Wandiga ◽  
M.O Onani
Keyword(s):  
Superparamagnetic Iron Oxide ◽  
Active Centre ◽  
Dna Targeting ◽  
Advantages And Disadvantages ◽  
Cancer Pathogenesis ◽  
Ongoing Work ◽  
Target Dna ◽  
Anti Cancer ◽  
New Research ◽  
Innovative Drugs

: Modern anticancer drugs target DNA specifically on rapid division of malignant cells. One downside of this approach is that they also target other rapidly dividing healthy cells such as those involved in hair growth leading to serious toxic side effects and hair loss. Therefore, it would be better to develop novel agents that address cellular signalling mechanisms unique to cancerous cells, and new research is now focussing on such approaches. Although the classical chemotherapy area involving DNA as the set target continues to produce important findings, nevertheless, a distinctly discernible emerging trend is the divergence from the cisplatin operation model that uses the metal as the primary active centre of the drug. Many successful anti-cancer drugs present are associated with elevated toxicity levels. Cancers also develop immunity against most therapies and the area of cancer research can therefore be seen as an area with a high unaddressed need. Hence, ongoing work into cancer pathogenesis is important to create accurate preclinical tests which can contribute to the development of innovative drugs to manage and treat cancer. Some of the emergent frontiers utilizing different approaches include nanoparticles delivery, use of quantum dots, metal complexes, tumour ablation, magnetic hypothermia and hyperthermia by use of Superparamagnetic Iron oxide Nanostructures, pathomics and radiomics, laser surgery and exosomes. This review summarizes these new approaches in good detail giving critical views with necessary comparisons. It also delves into what they carry for the future including their advantages and disadvantages.

scholarly journals Mesenchymal Stem Cells Do Not Lose Direct Labels Including Iron Oxide Nanoparticles and DFO-89Zr Chelates through Secretion of Extracellular Vesicles

Membranes ◽  
2021 ◽  
Vol 11 (7) ◽  
pp. 484
Author(s):  
Yue Gao ◽  
Anna Jablonska ◽  
Chengyan Chu ◽  
Piotr Walczak ◽  
Miroslaw Janowski
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Iron Oxide ◽  
Iron Oxide Nanoparticles ◽  
Extracellular Vesicles ◽  
Superparamagnetic Iron Oxide ◽  
Cell Labeling ◽  
Oxide Nanoparticles ◽  
Stem Cell Delivery ◽  
Cellular Labeling

Rapidly ageing populations are beset by tissue wear and damage. Stem cell-based regenerative medicine is considered a solution. Years of research point to two important aspects: (1) the use of cellular imaging to achieve sufficient precision of therapeutic intervention, and the fact that (2) many therapeutic actions are executed through extracellular vesicles (EV), released by stem cells. Therefore, there is an urgent need to interrogate cellular labels in the context of EV release. We studied clinically applicable cellular labels: superparamagnetic iron oxide nanoparticles (SPION), and radionuclide detectable by two main imaging modalities: MRI and PET. We have demonstrated effective stem cell labeling using both labels. Then, we obtained EVs from cell cultures and tested for the presence of cellular labels. We did not find either magnetic or radioactive labels in EVs. Therefore, we report that stem cells do not lose labels in released EVs, which indicates the reliability of stem cell magnetic and radioactive labeling, and that there is no interference of labels with EV content. In conclusion, we observed that direct cellular labeling seems to be an attractive approach to monitoring stem cell delivery, and that, importantly, labels neither locate in EVs nor affect their basic properties.

Monodispersed polymer encapsulated superparamagnetic iron oxide nanoparticles for cell labeling

Polymer ◽  
2016 ◽  
Vol 106 ◽  
pp. 238-248 ◽  
Author(s):  
Duc Nguyen ◽  
Binh T.T. Pham ◽  
Vien Huynh ◽  
Byung J. Kim ◽  
Nguyen T.H. Pham ◽  
...  
Keyword(s):  
Iron Oxide ◽  
Iron Oxide Nanoparticles ◽  
Superparamagnetic Iron Oxide ◽  
Superparamagnetic Iron Oxide Nanoparticles ◽  
Cell Labeling ◽  
Oxide Nanoparticles

Enhancing the Mobile User Experience Through Colored Contrasts

2019 ◽  
pp. 863-876
Author(s):  
Jean-Éric Pelet ◽  
Basma Taieb
Keyword(s):  
Behavioral Intentions ◽  
Negative Contrast ◽  
Positive Contrast ◽  
Mobile User ◽  
Structural Equations ◽  
Color Contrast ◽  
Affective States ◽  
Positive Effects ◽  
Green Background ◽  
Managerial Implications

This chapter examines the effects of the mobile-phone website colored contrasts and the affective states of the consumer (emotions and moods) and trust, respectively, on intention to revisit, buy on, and recommend the mobile website. For this purpose, a factorial plan 2x2 was developed, and a mobile website, with two different alternatives, was designed especially for the experiment: positive contrast (yellow text on green background) and negative contrast (green text on yellow background). The research was conducted on French consumers. Three-hundred twelve valid responses were collected through online and personal survey questionnaires. Data was analysed using the method of structural equations. The results show the significant effects of mobile website's color contrast on behavioral intentions. Perceived dominance and trust towards the website have positive effects on behavioral intentions, whereas mood has non-significant effects on behavioral intentions. Managerial implications are discussed.

Enhancing the Mobile User Experience Through Colored Contrasts

2018 ◽  
pp. 6070-6082
Author(s):  
Jean-Éric Pelet ◽  
Basma Taieb
Keyword(s):  
Behavioral Intentions ◽  
Negative Contrast ◽  
Positive Contrast ◽  
Mobile User ◽  
Structural Equations ◽  
Color Contrast ◽  
Affective States ◽  
Positive Effects ◽  
Green Background ◽  
Managerial Implications

This study examines the effects of the mobile-phone website colored contrasts and the affective states of the consumer (emotions and moods) and trust respectively on intention to revisit, buy on and recommend the mobile website. For this purpose, a factorial plan 2x2 was developed and a mobile website, with two different alternatives, was designed especially for the experiment: positive contrast (yellow text on green background) and negative contrast (green text on yellow background). The research was conducted on French consumers. 312 valid responses were collected through online and personal survey questionnaires. Data was analysed using the method of structural equations. The results show the significant effects of mobile website's color contrast on behavioral intentions. Perceived dominance and trust towards the website have positive effects on behavioral intentions, whereas mood has non-significant effects on behavioral intentions. Managerial implications are discussed.

Manganese–gold nanoparticles as an MRI positive contrast agent in mesenchymal stem cell labeling

2012 ◽  
Vol 14 (4) ◽  
Author(s):  
Simona E. Hunyadi Murph ◽  
Stephanie Jacobs ◽  
Jimei Liu ◽  
Tom C.-C. Hu ◽  
Matthew Siegfired ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Contrast Agent ◽  
Positive Contrast ◽  
Cell Labeling

Stem cell tracking using effective self-assembled peptide-modified superparamagnetic nanoparticles

Nanoscale ◽  
2018 ◽  
Vol 10 (34) ◽  
pp. 15967-15979 ◽  
Author(s):  
Lei Gu ◽  
Xue Li ◽  
Jing Jiang ◽  
Gang Guo ◽  
Haoxing Wu ◽  
...  
Keyword(s):  
Stem Cell ◽  
Cell Tracking ◽  
Superparamagnetic Iron Oxide ◽  
Mri Contrast Agents ◽  
Superparamagnetic Nanoparticles ◽  
Cell Labeling ◽  
Oxide Nanoparticles ◽  
Resonance Imaging ◽  
Mri Contrast ◽  
Magnetic Resonance Imaging Mri

Peptide modified superparamagnetic iron oxide nanoparticles (SPIONs) have been developed as excellent magnetic resonance imaging (MRI) contrast agents for stem cell labeling and tracking due to their biocompatibility.

Sign in / Sign up

Export Citation Format

Share Document