scholarly journals Spectrophotometric Determination of Some Non-Sedating Antihistamines Using Erythrosine B

2013 ◽  
Vol 2013 ◽  
pp. 1-9 ◽  
Author(s):  
Michael E. El-Kommos ◽  
Samia M. El-Gizawy ◽  
Noha N. Atia ◽  
Noha M. Hosny
Keyword(s):  
Solvent Extraction ◽  
Complex Formation ◽  
Spectrophotometric Method ◽  
Dosage Forms ◽  
Ion Pair ◽  
Pharmaceutical Dosage Forms ◽  
Erythrosine B ◽  
Ich Guidelines ◽  
Sensitive Spectrophotometric Method

A simple and sensitive spectrophotometric method has been developed for the determination of cetirizine (I), ebastine (II), fexofenadine (III), ketotifen (IV), and loratadine (V) based on ion-pair complex formation with erythrosine B. The pink color of the produced complex was measured at 550 nm without solvent extraction. Appropriate conditions were established by studying the color reaction between erythrosine B and the studied drugs to obtain the maximum sensitivity. Beer-Lambert's law is obeyed in the concentration ranges 1–7, 1–8, and 1–6 g/mL for (I, IV), (II, III), and (V), respectively. The method was validated according to ICH guidelines. The suggested method is applicable for the determination of the five investigated drugs in bulk and pharmaceutical dosage forms with excellent recoveries.

SIMULTANEOUS ESTIMATION AND VALIDATION OF RAMIPRIL AND TELMISARTAN BY UV SPECTROPHOTOMETRY

INDIAN DRUGS ◽  
2014 ◽  
Vol 51 (09) ◽  
pp. 31-35
Author(s):  
R Rambabu ◽  
◽  
S Vidyadhara ◽  
J Subbarao
Keyword(s):  
Spectrophotometric Method ◽  
Correlation Coefficients ◽  
Simultaneous Equation ◽  
Dosage Forms ◽  
Simultaneous Estimation ◽  
Uv Spectrophotometry ◽  
Intermediate Precision ◽  
Pharmaceutical Dosage Forms ◽  
Sensitive Spectrophotometric Method

A simple and sensitive spectrophotometric method for the determination of ramipril and telmisartan in pharmaceutical dosage forms has been developed. The absorption maxima were found at 220nm for ramipril and 297nm for telmisartan using 0.1N NaOH as solvent. Beer’s law was obeyed for both the drugs in the concentration range of 2-10μg/ml with correlation coefficients 0.999 for both ramipril and telmisartan. The limits of detection for ramipril and telmisartan were found to be 0.142 and 0.405μg/mL respectively and the limits of quantitation were 0.43 and 1.22μg/mL. Accuracy of the method was verified by performing recovery studies using simultaneous equation method and found to be 98.33 to 99.54%w/w for ramipril and 99.36 to 99.82 %w/w for telmisartan. %RSD of repeatability and intermediate precision studies were found to be <2 for both the drugs. Ruggedness of the method was checked by changing analyst worked and instrument used. In both the cases, the %RSD was found to be less than 2.

scholarly journals Spectrophotometric analysis of Azithromycin and its pharmaceutical dosage forms: Comparison between Spectrophotometry and HPLC

2015 ◽  
Vol 12 (2) ◽  
pp. 171-179 ◽  
Author(s):  
Nahid Sharmin ◽  
Nazia Sultana Shanta ◽  
Sitesh C Bachar
Keyword(s):  
Statistical Analysis ◽  
Spectrophotometric Method ◽  
Dosage Form ◽  
Dosage Forms ◽  
Spectrophotometric Analysis ◽  
Pharmaceutical Dosage Form ◽  
Pharmaceutical Dosage Forms ◽  
Ich Guidelines ◽  
Good Accordance

A simple, reliable, precise and sensitive UV-spectrophotometric method was developed and validated for the estimation of azithromycin in pharmaceutical dosage form and compared with official USP 2010 method. The proposed method utilizes the oxidation of azithromycin with potassium permanganate to liberate formaldehyde. This formaldehyde reacts with acetone-ammonium reagent and produces yellow colored chromogen 3,5-diacetyl-2,6-dihydrolutidine. The colored solution exhibited a maximum absorption at 412 nm which can be detected with UVspectrophotometer. The method was found linear over the concentration range 80% to 120% of the working concentration (R2=0.999). The intra- and inter-day RSD (n = 6) was ? 2.0%. The developed method was validated according to ICH guidelines and values of accuracy, precision and other statistical analysis were found to be in good accordance with the prescribed values. The proposed method was successfully applied for determination of azithromycin and the results have been compared with HPLC and thus enabling the utility of this new method for routine analysis azithromycin in pharmaceutical dosage forms DOI: http://dx.doi.org/10.3329/dujps.v12i2.21981 Dhaka Univ. J. Pharm. Sci. 12(2): 171-179, 2013 (December)

scholarly journals Isoabsorptive Point Method for Simultaneous Determination of Paracetamol and Orphenadrine Citrate in Their Combined Pharmaceutical Dosage Forms

2020 ◽  
pp. 1-4
Author(s):  
Mahmoud Mohammed Mohammed Sebaay ◽  
Amr A. Mattar ◽  
Mahmoud Mohammed Mohammed Sebaay
Keyword(s):  
Simultaneous Determination ◽  
Spectrophotometric Method ◽  
Dosage Forms ◽  
Pharmaceutical Formulation ◽  
Point Method ◽  
Pharmaceutical Dosage Forms ◽  
Statistical Comparison ◽  
Advantages And Disadvantages ◽  
Ich Guidelines

A simple, specific, accurate and precise spectrophotometric method was settled for simultaneous determination of paracetamol and orphenadrine citrate in their pure form and in their pharmaceutical formulation. Isoabsorptive point technique has been used in simultaneous determination of both drugs without prior separation. Isoabsorptive point method parameters were validated according to ICH guidelines in which accuracy, precision, repeatability and robustness were found in accepted limits. Advantages and disadvantages of Isoabsorptive point were discussed and statistical comparison between the proposed method and the reference one was also performed.

scholarly journals Generic Nonextractive Spectrophotometric Method for Determination of 4-Quino-Naphthollone Antibiotics by Formation of Ion-Pair Complexes with -Naphthol

2006 ◽  
Vol 89 (2) ◽  
pp. 334-340 ◽  
Author(s):  
Ibrahim A Darwish ◽  
Ibrahim H Refaat ◽  
Hassan F Askal ◽  
Mostafa A Marzouq
Keyword(s):  
Spectrophotometric Method ◽  
Dosage Forms ◽  
Ion Pair ◽  
Water Soluble ◽  
Experimental Conditions ◽  
Pharmaceutical Dosage Forms ◽  
Sulfuric Acid Medium ◽  
Quinolone Antibiotics ◽  
Relative Standard

Abstract This paper describes the development of a generic spontaneous nonextractive spectrophotometric method for determination of 13 pharmaceutically important 4-quinolone antibiotics. The method was based on the formation of yellow-colored water-soluble ion-pair complexes between 2% (w/v) -naphthol reagent and each of the studied drugs in sulfuric acid medium at room temperature. The formed ion-pair chromogens have maximum absorption peaks in the range of 365391 nm. The concentrations of the reagents and the experimental conditions affecting the reaction were optimized. Under the optimum conditions, linear relationships with good linear coefficients (0.99870.9995) were found between the absorbance and concentration of the investigated drugs in the range of 10350 g/mL. The assay limits of detection and quantitation were 19.9 and 3.432.9 g/mL, respectively. The precision of the method was satisfactory; the values of relative standard deviations did not exceed 2%. The proposed method was successfully applied to the analysis of the investigated drugs in pure and pharmaceutical dosage forms with good accuracy and precision; the percentages of label claim ranged from 97.8102.8 0.351.60%. The results obtained by the proposed spectrophotometric method were comparable with those obtained by the official or reported methods. The proposed method is superior to all the previously reported ion-pair formation-based methods in terms of simplicity because it did not involve extraction procedures for the ion-pair complex. Therefore, this method might be recommended for routine use in quality control laboratories for analysis of the investigated 4-quinolone antibiotics in their pure forms, as well as in pharmaceutical dosage forms.

scholarly journals Pyrocatechol Violet in Pharmaceutical Analysis Part II. A Spectrophotometric Method for the Determination of Paracetamol in Pure and in Pharmaceutical Dosage Forms

2001 ◽  
Vol 69 (2) ◽  
pp. 179-188 ◽  
Author(s):  
Alaa Amin
Keyword(s):  
Spectrophotometric Method ◽  
Molar Absorptivity ◽  
Dosage Forms ◽  
Ion Pair ◽  
Official Method ◽  
Pyrocatechol Violet ◽  
Pharmaceutical Dosage Forms ◽  
Selective Determination ◽  
Significant Difference

A spectrophotometric method for the selective determination of paracetamol based on its reaction with pyrochatechol violet under basic conditions to form an ion-pair complex is described. The absorption maximum of the coloured ion-pair formed is observed at 652 nm and the molar absorptivity is 4.54 x10-3l mol-1 cm-1. Beer's law is obeyed over the concentration range 0.5-34.0 μg ml-1, while that obtained using Ringbom method is in the range 3.5 -32.0 μg ml-1. There is no interference from common additives, excipients and commercial drugs present in their formulations suggesting a highly selective procedure compared with others. Statistical analysis of the obtained results showed that there is, no significant difference and absence of any systematic error in the method compared with the official one. The method is simple, rapid and convenient and was applied successfully to the determination of paracetamol in pure and in its dosage forms compared with the official method.

scholarly journals New Spectrophotometric Method for the Determination of Bosentan - An Anti-Hypertensive Agent in Pharmaceutical Dosage Forms

2012 ◽  
Vol 9 (2) ◽  
pp. 700-704 ◽  
Author(s):  
A. Narendra ◽  
D. Deepika ◽  
M. Mathrusri Annapurna
Keyword(s):  
Spectrophotometric Method ◽  
Sulfonic Acid ◽  
Pharmaceutical Formulations ◽  
Solvent Mixture ◽  
Molar Absorptivity ◽  
Dosage Forms ◽  
Ultraviolet Region ◽  
Pharmaceutical Dosage Forms ◽  
Ich Guidelines

A new, simple and sensitive spectrophotometric method in ultraviolet region has been developed for the determination of Bosentan in bulk and in pharmaceutical formulations. Bosentan exhibits absorption maxima at 273 nm with apparent molar absorptivity of 1.3293×104L/mol.cm in methanol and octane 1-sulfonic acid solvent mixture. Beer's law was found to be obeyed in the concentration range of 0.1-100 μg/mL. The method was validated as per the ICH guidelines.

Spectrophotometric Determination of Alendronate Sodium by using Sodium-1,2-Naphthoquinone-4-Sulphonate

2012 ◽  
Vol 4 (4) ◽  
pp. 1563-1568
Author(s):  
Sagar Suman Panda ◽  
Ravi Kumar B V V ◽  
D Patanaik
Keyword(s):  
Spectrophotometric Method ◽  
Absorption Maximum ◽  
Dosage Form ◽  
Dosage Forms ◽  
Alendronate Sodium ◽  
Colored Complex ◽  
Pharmaceutical Dosage Forms ◽  
Recovery Study ◽  
Assay Conditions

A simple, precise and accurate spectrophotometric method was developed for analysis of the osteoporesis drug alendronate sodium (ALS). The method is based on reaction of the drug with sodium-1,2-naphthoquinone-4-sulphonate (NQS) in presence of alkali to form a brown colored complex giving absorption maximum at 525 nm. The drug obeyed Beer’s law in the range of 5-70 µg/ml with a correlation coefficient of 0.999. The LOD and LOQ values are 1.7 µg/ml and 5.0 µg/ml, respectively. The average recoveries for recovery study were found to be in the range of 99.37%-100.46%. The R.S.D. values for intraday and inter-day precision were found to be 0.48 and 0.62, respectively. The optimized assay conditions were applied successfully for determination of ALS in pharmaceutical dosage forms. No interference was observed from the excipients present in the dosage form. The method is statistically validated as per the ICH requirements.  

scholarly journals Development and validation of a new spectrofluorimetric method for the determination of some beta-blockers through fluorescence quenching of eosin Y. Application to content uniformity test

2016 ◽  
Vol 14 (1) ◽  
pp. 258-266 ◽  
Author(s):  
Sayed M Derayea ◽  
Mahmoud A Omar ◽  
Mohamed Aboel-Kasem Abdel-Lateef ◽  
Ahmed I. Hassan
Keyword(s):  
Fluorescence Quenching ◽  
Beta Blockers ◽  
Dosage Forms ◽  
Ion Pair ◽  
Content Uniformity ◽  
Eosin Y ◽  
Pharmaceutical Dosage Forms ◽  
Quenching Effect ◽  
Spectrofluorimetric Method

AbstractA simple, rapid, sensitive and economic spectrofluorimetric method has been developed and validated for determination of some β-adrenergic blocking agents namely; betaxolol hydrochloride (BTX), carvedilol (CAR), labetalol hydrochloride (LBT), nebivolol hydrochloride (NEB) and propranolol hydrochloride (PRO). The method is based on the quenching effect of the cited drugs on the fluorescence intensity of eosin Y at pH 3.4 (acetate buffer). The fluorescence quenching is due to the formation of an ion-pair complex and was measured without extraction at 545 nm (λex. 301.5 nm). The factors affecting the formation of the ion-pair complex were carefully studied and optimized. Under the optimal conditions, the linear ranges for the relationship between the fluorescence quenching value and the concentration of the investigated drugs were 100-2500, 150-2500 and 50-2250 ng mL-1 for (BTX, CAR), (LBT, NEB) and (PRO) respectively. The method was validated according to ICH guidelines and was applied for determination of the cited drugs in pharmaceutical dosage forms with excellent recoveries. In addition, content uniformity testing of some commercial dosage forms was checked by the proposed method.

scholarly journals DEVELOPMENT AND VALIDATION OF ULTRAVIOLET-SPECTROPHOTOMETRIC METHOD FOR DETERMINATION OF FEBUXOSTAT FOR CONDUCTING IN-VITRO QUALITY CONTROL TESTS IN BULK AND PHARMACEUTICAL DOSAGE FORMS

2018 ◽  
Vol 11 (9) ◽  
pp. 115
Author(s):  
Jaspreet Kaur ◽  
Daljit Kaur ◽  
Sukhmeet Singh
Keyword(s):  
Spectrophotometric Method ◽  
Limit Of Detection ◽  
Pharmaceutical Formulations ◽  
Dosage Forms ◽  
Pharmaceutical Dosage Forms ◽  
Relative Standard ◽  
Limit Of Quantitation ◽  
Development And Validation

Objective: A simple, accurate, and selective ultraviolet-spectrophotometric method has been developed for the estimation of febuxostat in the bulk and pharmaceutical dosage forms.Method: The method was developed and validated according to International Conference on Harmonization (ICH Q2 R1) guidelines. The developed method was validated statistically with respect to linearity, range, precision, accuracy, ruggedness, limit of detection (LOD), limit of quantitation (LOQ), and recovery. Specificity of the method was demonstrated by applying different stressed conditions to drug samples such as acid hydrolysis, alkaline hydrolysis, oxidative, photolytic, and thermal degradation.Results: The study was conducted using phosphate buffer pH 6.8 and λmax was found to be 312 nm. Standard plot having a concentration range of 1–10 μg/ml showed a good linear relationship with R2=0.999. The LOD and LOQ were found to be 0.118 μg/ml and 0.595 μg/ml, respectively. Recovery and percentage relative standard deviations were found to be 100.157±0.332% and <2%, respectively.Conclusion: Proposed method was successfully applicable to the pharmaceutical formulations containing febuxostat. Thus, the developed method is found to be simple, sensitive, accurate, precise, reproducible, and economical for the determination of febuxostat in pharmaceutical dosage forms.

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