scholarly journals Loranthus pulverulentus: A Potent Source of Natural Antioxidants and Alternative Medicine

2013 ◽  
Vol 2013 ◽  
pp. 1-7 ◽  
Author(s):  
Muhammad Asam Raza ◽  
Rukhsana Kausar ◽  
Faraz Ali Rana ◽  
Muhammad Danish ◽  
Durre Shahwar ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Natural Antioxidants ◽  
Stem Bark ◽  
Antioxidant Potential ◽  
Total Phenolic ◽  
Strong Correlations ◽  
Phenolic Contents ◽  
Total Phenolic Contents ◽  
Total Antioxidant

This study was designed to evaluate the antioxidant potential ofLoranthus pulverulentus. Stem bark, leaves, and seeds ofLoranthus pulverulentuswere extracted in methanol:water (90 : 10) and partitioned with n-hexane, chloroform, ethyl acetate, and n-butanol successively using partition chromatography. Total phenolic contents and antioxidant potential were checked using standard protocols. Total phenolic contents of all extracts were determined, using Folin–Ciocalteu reagent, and ranged between 151 ± 2.1 and 396 ± 1.6 for stem bark, 137 ± 0.9 and 430 ± 2.2 for, and 39 ± 0.6 and 231 ± 1.7 for seeds. The antioxidant potential of extracts was evaluated; namely, DPPH, FRAP, and total antioxidant models. The ethyl acetate extract of stem-bark, leaves, and seeds showed the highest activity in DPPH (94.5 ± 2.1%, 96.30 ± 0.9%, and 92.30 ± 1.1%, IC5015.9 ± 0.5 μg, 14.5 ± 0.8, and 102.7 ± 1.3, resp.), FRAP (7.7 ± 0.6, 7.5 ± 0.7 and 6.6 ± 0.7, resp.), and total antioxidant (0.95 ± 0.09, 1.19 ± 0.09, and 0.686 ± 0.08, resp.). Strong correlations were observed between total phenols versus total antioxidant activity, DPPH, and FRAP withR2values ranging from 0.8185 to 0.9951 (stem-bark), 0.6728 to 0.8648 (leaves), and 0.8658 to 0.9910 (seed) which indicated that phenolic contents are the major constituents responsible for antioxidant activity.

scholarly journals Effect of Electrospinning Process on Total Antioxidant Activity of Electrospun Nanofibers Containing Grape Seed Extract

2019 ◽  
Vol 17 (1) ◽  
pp. 912-918 ◽  
Author(s):  
Rabia Faki ◽  
Oguz Gursoy ◽  
Yusuf Yilmaz
Keyword(s):  
Antioxidant Activity ◽  
Grape Seed Extract ◽  
Grape Seed ◽  
Electrospinning Process ◽  
Seed Extract ◽  
Total Phenolic ◽  
Total Antioxidant Activity ◽  
Phenolic Contents ◽  
Total Phenolic Contents ◽  
Total Antioxidant

AbstractElectrospinning is a common technique used for the production of nanofibers, and it is based on the fact that the electrically charged liquid polymer is positioned in a continuous fiber form on a grounded surface. Grape seed is rich in phenolic compounds and can be used as a dietary supplement or as a natural antioxidant source in diet. In this study, grape seed extract of Burdur Dimrit variety (Vitis vinifera L.) was electrospun with gelatin, polyvinyl alcohol (PVA) and PVA/β-cyclodextrin polymers to produce nanofibers with antioxidant activity. The aim of this study was to determine the effect of the electrospinning process on the total antioxidant activity and total phenolic contents of electrospun polymers with grape seed extracts. Total antioxidant activity of samples (by ABTS and DPPH assays) and total phenolic contents (Folin–Ciocalteu method) were determined before and after the electrospinning process of polymers with grape seed extract. Electrospinning with gelatin polymer decreased the antioxidant activity (ABTS assay) of nanofibers containing grape seed extract by 65% and their total phenolic contents by 7%. However, electrospinning treatment with PVA and PVA/β-cyclodextrin had no effect on the total antioxidant activity (ABTS and DPPH) and total phenolic substance contents of grape seed extract nanofibers.

scholarly journals Antioxidant activity of fungi isolated from soil of different areas of Punjab,India

2009 ◽  
Vol 1 (2) ◽  
pp. 123-128 ◽  
Author(s):  
Priyanka Chandra ◽  
Daljit Singh Arora
Keyword(s):  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Higher Plants ◽  
Reducing Power ◽  
Natural Antioxidants ◽  
Antioxidant Potential ◽  
Total Phenolic ◽  
Phenolic Contents ◽  
Reducing Power Assay ◽  
Set Up

The study was carried outto investigate the antioxidant activity of fungi isolated from soil of different areas of Punjab, India and compare their efficiency with a known antioxidant, ascorbic acid. The antioxidant potential of fungal extracts was quantified by DPPH and reducing power assay. Total phenolic contents were estimated using Folins-Ciocalteau (FC) reagent. Out of 113 fungal isolates selected, 51 were having antioxidant potential and these were further assayed quantitatively. All of these showed good activity against DPPH radical while 32 of these isolates demonstrated reducing potential also. In addition, some were equally good as ascorbic acid. The present study demonstrated potential of soil fungi to have antioxidant activity similar to plants and mushrooms. High phenolic content of fungi further highlight their significance as new sources of natural antioxidants. These fungi may provide easier set up for production and purification of natural antioxidants as compared to higher plants.

SKRINING FITOKIMIA DAN UJI AKTIVITAS ANTIOKSIDAN DAN ANTITUMOR 20 TUMBUHAN OBAT ASAL KABUPATEN MANOKWARI

2008 ◽  
Vol 7 (1) ◽  
Author(s):  
Bimo Budi Santoso
Keyword(s):  
Antioxidant Activity ◽  
Medicinal Plants ◽  
Antitumor Activity ◽  
Traditional Method ◽  
Extraction Efficiency ◽  
Phenolic Content ◽  
Antioxidant Activities ◽  
Total Phenolic ◽  
Phenolic Contents ◽  
Total Phenolic Contents

<strong><em>Flavanoid, steroid, and terpenoid contents of 20 Manokwari medicinal plants were analyzed.  The antioxidant activity, total phenolic contents and antitumor activity of 20 Manokwari medicinal plants were also evaluated. The result shows that 83% positif to flavanoid test, 59% positif to terpenoid test and only 25% positif steroid. Antioxidant activity and total phenolic contents evaluated using Ferric Thiocianate (FTC) and Folin-Ciocalteu methods respectively. Antioxidant activity and total phenolic contents of medicinal plants were extracted by the traditional method, boiling in water and also in 80% methanol. Twenty plants evaluated in both exstracts have significantly varies of antioxidant activities and phenolic contents,  A significant and linier correlation coefficient between the antioxidant activity and the total phenolic content was found in both aqueous (R<sup>2</sup>= 0,77) and methanol (R<sup>2</sup> = 0,85). Antitumor activity was tested using cell maurine P-388 and only 2 of medicinal plants are active to inhibit cell maurine P-388. Comparing extraction efficiency of the two methods, the methanol extracted phenolic compounds more efficiently, and antioxidant activity of the extract was higher.</em></strong>

scholarly journals POLYPHENOL CONTENT AND ANTIOXIDANT POTENTIAL OF Allophylus edulis (A. St.-Hil. et al.) Hieron. ex Niederl. AND Cupania vernalis Cambess. (SAPINDACEAE)

2021 ◽  
Vol 45 ◽  
Author(s):  
Andréa Michel Sobottka ◽  
Elisandra Tessaro ◽  
Suelen Maier da Silva ◽  
Marina Pedron ◽  
Lara Tortini Seffrin
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Skin Diseases ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Ethyl Acetate Fraction ◽  
Antioxidant Potential ◽  
Total Phenolic ◽  
Butanol Fraction ◽  
Degraded Ecosystems

ABSTRACT Allophylus edulis and Cupania vernalis (Sapindaceae) are Brazilian native trees used as medicinal plants for the treatment of respiratory, digestive, circulatory, and skin diseases. Ubiquitously distributed in the Brazilian territory, these species are indicated for mixed plantations aimed at the recovery of degraded ecosystems. In this study, the total phenolic content (TPC) and total flavonoid content (TFC), and the antioxidant activity of extracts and fractions obtained from A. edulis and C. vernalis leaves were assessed. The TPC and TFC was determined spectrophotometrically. Antioxidant activity was evaluated through radical scavenging activity of 2,2-diphenyl-1-picrylhydrazyl (DPPH). The extracts were obtained by two methods: maceration (method 1) and Soxhlet (method 2). Solvents of increasing polarity (hexane, dichloromethane, ethyl acetate, and n-butanol) were used to obtained the fractions. The results showed that the ethyl acetate fraction from A. edulis, obtained from the maceration method, had the highest TPC (442.0 ± 18.2 mg GAE g-1) and TFC (58.1 ± 0.4 mg RUE g-1), and antioxidant activity (EC50 = 43.6 ± 2.6 µg mL-1). By C. vernalis, superior results were obtained with the n-butanol fraction (TPC = 126.1 ± 5.8 mg GAE g-1, TFC = 37.7 ± 0.6 mg RUE g-1). The highest antioxidant potential was found in the crude hydroalcoholic extract (EC50 = 816.1 ± 50.9 µg mL-1) and butanol fraction (1,156.4 ± 3.8 µg mL-1). The results of this study show that the fractions obtained by maceration and liquid-liquid partition with more polar solvents (ethyl acetate and n-butanol) are the richest in TPC and TFC, and presented the greater antioxidant activity. Comparing the two plants, A. edulis showed the best results, with a high content of TPC, TFC, and antioxidant potential, and therefore may be used to treat diseases related to oxidative stress.

scholarly journals COMPARATIVE STUDY ON THE ANTIOXIDANT ACTIVITIES OF METHANOLIC AND AQUEOUS EXTRACTS OF TERMINALIA BELLERICA

2019 ◽  
pp. 103-110
Author(s):  
RASNA GUPTA ◽  
RAM LAKHAN SINGH ◽  
ANKIT GUPTA
Keyword(s):  
Antioxidant Activities ◽  
Natural Antioxidants ◽  
Total Phenolic ◽  
Aqueous Extracts ◽  
Fruit Pulp ◽  
Terminalia Bellerica ◽  
Phenolic Contents ◽  
Total Phenolic Contents ◽  
Methanolic Extracts ◽  
Better Than

Objective: The present study was undertaken to investigate the antioxidative activity of fruit pulp, seed and bark of T. bellerica. Methods: Methanol (70%) and water were used for the extraction, and the respective extracts were analyzed for total phenolic contents along with the antioxidant activities through different assays. Results: Amongst the tested extracts, methanolic extracts were found to be better than aqueous with the highest total phenolic contents (TPC). Methanolic fruit pulp showed lower IC50 for free radical (118.7 µg/ml), superoxide anion radical (77.65 µg/ml) and hydroxyl radical (73.76 µg/ml) scavenging activities. Methanolic fruit pulp also showed lower IC50 for lipid peroxidation (115.6 µg/ml) and ferric thiocyanate (184.98 µg/ml) assay than aqueous extracts in comparison to standard quercetin. Further, correlation between TPC and antioxidant studies revealed that phenolics are mainly responsible for antioxidant activity of T. bellerica. Conclusion: The current study suggests that the methanolic extract of T. bellerica could be a potential source of natural antioxidants for food and pharmaceutical companies.

Quantitative Analysis of Total Phenolic Contents and Antioxidant Activity of Fresh Field Natural Rubber Latex

2015 ◽  
Vol 354 (1) ◽  
pp. 265-272 ◽  
Author(s):  
Suwimon Siriwong ◽  
Adisai Rungvichaniwat ◽  
Pairote Klinpituksa ◽  
Khalid Hamid Musa ◽  
Aminah Abdullah
Keyword(s):  
Antioxidant Activity ◽  
Quantitative Analysis ◽  
Natural Rubber ◽  
Natural Rubber Latex ◽  
Total Phenolic ◽  
Rubber Latex ◽  
Phenolic Contents ◽  
Total Phenolic Contents
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